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Liu Y.,Beijing Normal University | Liu Y.,Capital Medical University | Tian M.,Capital Medical University | Wang X.,Capital Medical University | And 14 more authors.
PLoS ONE | Year: 2014

pConclusions/Significance: Our study provides a detailed characterization of the genotypic diversity of M. tuberculosis in Beijing. Combining spoligotyping and VNTR typing to study the genotyping of M. tuberculosis gave superior results than when these techniques were used separately. Our results indicated that Beijing family strains were still the most prevalent M. tuberculosis in Beijing. Moreover, VNTR typing analyzing of M. tuberculosis strains in Beijing was successfully accomplished using 12-locus (BJ) VNTR. This method used for strains genotyping from the Beijing metropolitan area was comparable. This study will not only provide TB researchers with valuable information for related studies, but also provides guidance for the prevention and control of TB in Beijing. Source


Che N.,Capital Medical University | Zhang Z.,Beijing Changping Center for Tuberculosis Control and Prevention | Zhang X.,Capital Medical University | Ding Z.,Capital Medical University | Li C.,Capital Medical University
Clinica Chimica Acta | Year: 2013

Background: Tuberculosis (TB) is a serious world-wide health problem, causing millions of deaths every year. Metabolomics is a relatively new approach to identify disease specific biomarkers. However, there is little information available on metabolite biomarkers in TB. In this study, we used gas chromatography/time-of-flight mass spectrometry (GC/TOF-MS) to identify serum metabolite biomarkers associated with the active state of TB. Methods: Potential biomarkers were selected by comparing serum metabolites in 10 healthy donors with 10. TB patients, and in 6. TB patients before and after treatment. Selected biomarkers were then validated using a larger population of samples from 120 healthy donors and 120. TB patients derived from different clinical backgrounds. Results: The 5-oxoproline level was consistently low in patients with active TB. Further validation in larger population of clinical samples showed that 5-oxoproline was associated with pathological damage of the lung but not with age, sex, or bacterial burden in TB patients. Conclusions: Serum 5-oxoproline may be a useful biomarker for active TB and pathological damage of the lung. © 2013 Elsevier B.V. Source


Che N.,Beijing Tuberculosis and Thoracic Tumor Research Institute | Li S.,Beijing Tuberculosis and Thoracic Tumor Research Institute | Li S.,Jilin Agricultural University | Gao T.,Beijing Changping Center for Tuberculosis Control and Prevention | And 13 more authors.
Clinica Chimica Acta | Year: 2010

Background: Human immunity-related GTPase M (IRGM) is found to play an important role in defense against intracellular pathogen Mycobacterium tuberculosis in vitro by regulating autophagy. To verify whether single nucleotide polymorphisms (SNPs) in the promoter region of IRGM gene are associated with tuberculosis (TB) 1.7 kb IRGM promoter region was sequenced and SNP analysis was conducted in TB patients and healthy controls. Methods: A simple and rapid procedure for extracting DNA from clotted-blood was developed in this study. A 1.7 kb IRGM promoter region was amplified and sequenced for nucleotide polymorphism search. Then, 3 SNPs were selected and analyzed in 216. TB patients and 275 healthy subjects by ligase detection reaction technique. Results: DNA extracted by our method was of high quality and suitable for PCR, sequencing, and genotyping. We identified 29 polymorphisms in the 1.7 kb IRGM promoter region, including 11 novel polymorphisms not yet reported. Large population analysis showed that frequencies of -1208A allele (P=0.031), -1208AA genotype (P=0.042), and -1208A/-1161C/-947C (P=0.035) and -1208G/-1161C/-947C (P=0.030) haplotypes in cases were significantly different from those in controls. Conclusions: In 1.7 kb IRGM promoter region, only -1208A/G polymorphism is associated with susceptibility to TB. © 2010 Elsevier B.V. Source


Ma Y.,Beijing Tuberculosis and Thoracic Tumor Research Institute | Ma Y.,Capital Medical University | Ma Y.,Clinical Center on Tuberculosis | Liu Y.H.,Beijing Tuberculosis and Thoracic Tumor Research Institute | And 7 more authors.
Biomedical and Environmental Sciences | Year: 2016

Interleukin 8 (IL8) is an important chemokine that elicits host immune response against tuberculosis (TB). However, whether there is an association between IL8 gene polymorphism and TB susceptibility in the Chinese population is unknown. IL8 gene was amplified and sequenced to search for nucleotide polymorphisms among the Chinese population. Four single nucleotide polymorphisms (SNPs) were identified, selected, and analyzed in a cohort of 438 patients with TB and 536 healthy controls. Allelic, genotypic, and haplotypic analysis demonstrated that the distribution of the four IL8 SNPs between patients with TB and healthy controls was not significantly different (P>0.05). The four IL8 SNPs detected in this study were not associated with TB susceptibility in the Chinese population. Secretion of IL8 by peripheral blood cells was greatly stimulated upon exposure to Mycobacterium tuberculosis whole cell extract, but such enhanced secretion was not associated with the IL8 rs4073 alleles. © 2016 The Editorial Board of Biomedical and Environmental Sciences. Source

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