Li X.,Chinese Academy of Agricultural Sciences |
Li X.,Beijing Challenge Bio technology Ltd Company |
Zhao J.,Chinese Academy of Agricultural Sciences |
Shi P.,Chinese Academy of Agricultural Sciences |
And 4 more authors.
Applied Biochemistry and Biotechnology | Year: 2013
A novel β-glucosidase gene, bgl1G5, was cloned from Phialophora sp. G5 and successfully expressed in Pichia pastoris. Sequence analysis indicated that the gene consists of a 1,431-bp open reading frame encoding a protein of 476 amino acids. The deduced amino acid sequence of bgl1G5 showed a high identity of 85 % with a characterized β-glucosidase from Humicola grisea of glycoside hydrolase family 1. Compared with other fungal counterparts, Bgl1G5 showed similar optimal activity at pH 6.0 and 50°C and was stable at pH 5.0-9.0. Moreover, Bgl1G5 exhibited good thermostability at 50°C (6 h half-life) and higher specific activity (54.9 U mg-1). The Km and Vmax values towards p-nitrophenyl β-D-glucopyranoside (pNPG) were 0.33 mM and 103.1 μmolmin-1mg-1, respectively. The substrate specificity assay showed that Bgl1G5 was highly active against pNPG, weak on p-nitrophenyl β-Dcellobioside (pNPC) and p-nitrophenyl-β-D-galactopyranoside (ONPG), and had no activity on cellobiose. This result indicated Bgl1G5 was a typical aryl β-glucosidase. © Springer Science+Business Media New York 2013.
Tang H.O.,Chinese Academy of Agricultural Sciences |
Gao X.H.,Chinese Academy of Agricultural Sciences |
Ji F.,Chinese Academy of Agricultural Sciences |
Tong S.,Beijing Challenge Bio technology Ltd Company |
Li X.J.,Beijing Challenge Bio technology Ltd Company
Journal of Applied Poultry Research | Year: 2012
An experiment was conducted to assess the effects of a novel thermostable phytase in male broiler chicks (Ross 308) fed available P (AP)-deficient diets on growth performance and bone mineralization. The treatments consisted of 8 experimental diets: 1 positive control diet containing an adequate level of AP, 1 negative control diet deficient in AP, and 6 diets with the same level of AP as in the negative control but supplemented with different levels of phytase (250, 500, 750, 1,000, 1,250, and 1,500 phytase units/kg of diet). The addition of phytase significantly improved (P < 0.05) BW gain, feed intake, FCR, toe ash, tibia ash, and tibia P of broilers compared with those fed the negative control diet. No significant differences (P > 0.05) were found in FCR and bone mineralization among the broilers fed different levels of phytase and those fed the positive control diet. In conclusion, normal growth performance and bone mineralization were maintained in broilers fed AP-deficient diets supplemented with thermostable phytase. ©2012 Poultry Science Association, Inc.
Tang H.,Chinese Academy of Agricultural Sciences |
Tang H.,Beijing Challenge Bio technology Ltd Company |
Yao B.,Chinese Academy of Agricultural Sciences |
Gao X.,Chinese Academy of Agricultural Sciences |
And 3 more authors.
South African Journal of Animal Sciences | Year: 2016
The experiment was conducted to investigate the effects of diets supplemented with glucose oxidase (GOD) on growth performance, serum parameters and faecal microflora of piglets. One hundred and twelve piglets (35 days old) were randomly assigned to two groups (four replicates per group, half male and half female, and 14 piglets per replicate) and fed a diet with or without 100 U GOD per kg, for 35 days. Feeding GOD caused a higher average daily weight gain and feed intake, and lower feed conversion ratio (FCR) of piglets. No significant difference was observed in the reference values of serum biochemical parameters between the groups fed with or without GOD. The contents of triiodothyronine, thyroxine and growth hormone of piglets fed GOD were higher than those of the control. Moreover, GOD supplementation suppressed the concentration of faecal Salmonella. The results showed that supplementation of GOD to diets promoted growth performance, increased the contents of growth and development-related hormones, and improved the faecal microflora of growing piglets.