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Jiang Y.,Chinese National Institute for Communicable Disease Control and Prevention | Liu H.C.,Chinese National Institute for Communicable Disease Control and Prevention | Zheng H.J.,Chinese National Human Genome Center at Shanghai | Tang B.,Chinese National Human Genome Center at Shanghai | And 10 more authors.
Biomedical and Environmental Sciences

Objective: To evaluate four candidate variable number tandem repeat (VNTR) loci for genotyping Mycobacterium tuberculosis complex strains. Methods: Genomic sequences for two M. tuberculosis strains (CCDC5079 and CCDC5180) were generated, and using published sequence data, four candidate VNTR loci were identified. The VNTRs were used to genotype 225 Chinese clinical M. tuberculosis complex strains. The discriminatory power of the VNTRs was evaluated using BioNumerics 5.0 software. Results: The Hunter-Gaston Index (HGI) for BJ1, BJ2, BJ3, and BJ4 loci was 0.634, 0.917, 0.697, and 0.910, respectively. Combining all four loci gave an HGI value of 0.995, thus confirming that the genotyping had good discriminatory power. The HGI values for BJ1, BJ2, BJ3, and BJ4, obtained from Beijing family strain genotyping, were 0.447, 0.878, 0.315, and 0.850, respectively. Combining all four loci produced an HGI value of 0.988 for genotyping the Beijing family strains. We observed unique patterns for M. bovis and M. africanum strains from the four loci. Conclusion: We have shown that the four VNTR loci can be successfully used for genotyping M. tuberculosis complex strains. Notably, these new loci may provide additional information about Chinese M. tuberculosis isolates than that currently afforded by established VNTR loci typing. © 2012 The Editorial Board of Biomedical and Environmental Sciences. Source

Zhang D.,Chinese PLA General Hospital | Wang S.,Chinese PLA General Hospital | Zhu L.,Chinese PLA General Hospital | Tian Y.,Chinese PLA General Hospital | And 4 more authors.
BioMed Research International

We cocultured calycosin with human hepatocellular carcinoma cell line (BEL-7402) to investigate the effect on cell proliferation. Calycosin can markedly block the cell growth in G1 phase (P<0.01) on the IC 50 concentration. There were seventeen genes involved in cell-cycle regulation showing differentially expressed in treated cells detected by gene chip. Eight genes were upregulated and nine genes were downregulated. Downregulated TFDP-1, CDKN2D, and SPK2 and upregulated CDC2 and CCNB1 might affect cell cycle of tumor cells. Furthermore, we checked the transcription pattern using 2D gel method to find different expression of proteins in human hepatocellular carcinoma cells after exposure to calycosin. Fourteen proteins were identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Twelve proteins expression were increased such as transgelin 2, pyridoxine 5′-phosphate, stress-induced-phosphoprotein 1, peroxiredoxin 1, endoplasmic reticulum protein 29, and phosphoglycerate mutase 1. Only thioredoxin peroxidase and high-mobility group box1 proteins' expression decreased. Both genes and proteins changes might be relate to the mechanism of antitumor effect under treatment of calycosin. In conclusion, calycosin has a potential effect to inhibit the BEL-7402 cell growth by inhibiting some oncogene expression and increasing anticancer genes expression, what is more, by blocking cell cycle. © 2013 Dongqing Zhang et al. Source

Liu H.,Chinese National Institute for Communicable Disease Control and Prevention | Liu H.,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases | Jiang Y.,Chinese National Institute for Communicable Disease Control and Prevention | Jiang Y.,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases | And 14 more authors.

The mycobacterial antigen PstS1 is a highly immunogenic and immunostimulatory component of the mycobacterial cell membrane and a good candidate for the diagnosis and vaccination against tuberculosis. Here we selected 180 clinical isolates of Mycobacterium tuberculosis complex (MTBC) in China and 11 different Bacille Calmette Guerin (BCG) strains, amplified the gene of the PstS1 antigen and compared the sequences with those of four other Mycobacterium bovis and BCG strains from the NCBI genome website. Some of the mutations, especially 2 frameshift mutations, occurred in the PstS1antigen, which may have resulted in the protein function alteration and ongoing immune evasion. A unique single nucleotide polymorphism of the M. bovis and BCG strains was found in this antigen and may be useful for differentiating M. bovis and BCG strains from M. tuberculosis strains. © 2013 Published by Elsevier Ltd. Source

Zhu Y.,Peking University | Wei J.,Beijing Center for Diseases Control and Prevention | Huang L.,Dongcheng District Center for Diseases Control and Prevention | Wang S.,Yanqing County Center for Diseases Control and Prevention | And 2 more authors.
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences

OBJECTIVE: To compare the differences of children's health in different area, and to confirm if the prevalence of respiratory diseases and symptoms among children are closely associated with the air pollution.METHODS: A cross-sectional study was conducted in an urban area A and a suburban area B with different levels of air pollution in Beijing. Using a cluster sampling method, we recruited 4 564 children from 3 primary schools in urban A and 4 primary schools in suburban B. Respiratory symptoms were investigated using an international standardized questionnaire including characteristics of children, living conditions, respiratory diseases and symptoms and situation of parents. The concentrations of air pollutants for recent five years were obtained from Reports on the Quality of the Beijing Environment. SPSS 16.0 was used to analyze data.RESULTS: The prevalence of cough, persistent cough, phlegm, persistent phlegm, wheeze and asthma in A area were higher than those in B area [(62.2% vs. 59.9%), (6.3% vs. 3.1%), (42.4% vs. 37.4%),(3.6% vs. 2.4%),(13.3% vs. 9.9%)and(9.5% vs. 5.4%)]. Except for cough, cough with cold, cough without cold, the prevalence of respiratory diseases and symptoms in A area were significantly higher than those in B area (P<0.05). Logistic regression analysis showed the prevalence of persistent cough, phlegm without cold, asthma in A area were significantly higher than those in B area (P<0.05).CONCLUSION: Respiratory diseases and symptoms among school-age children were closely associated with the level of air pollution. Source

Song B.,Beijing Center for Diseases Control and Prevention | Song B.,Agricultural University of Hebei | Ding X.,Beijing Center for Diseases Control and Prevention | Ding X.,Capital Medical University | And 3 more authors.
Chinese Journal of Chromatography (Se Pu)

Benzalkonium chloride (BAC) is a mixture of alkyl substituted benzyl dimethylammonium chloride homologs (C12-BAC, C14-BAC and C16-BAC). Chlorhexidine acetate is a widely used effective component in compound chemical disinfectants. A method for the simultaneous determination of chlorhexidine acetate and benzalkonium chloride in compound chemical disinfectants by capillary electrophoresis (CE) was established. The CE analysis was carried out using an uncoated capillary with 50μm i.d. and 37 cm total length. The running buffer was 150mmol/L NaH2PO4-62.5mmol/L H3PO4 (pH 2.5) containing 40% (v/v) acetonitrile. The sample medium was 50 mmol/L acetic acid-acetonitrile (1.1, v/v). The detection wavelength was 214nm. The factors such as the buffer concentration and pH, the content of acetonitrile, which influenced the separation and accurate assay of compound chemical disinfectants were investigated in detail. The intra-day and inter-day precisions of the method were below 3.0% and 3.7%, respectively. The limits of detection (LOD, signal to noise ratio (S/N) = 3) for chlorhexidine acetate, C12-BAC, C14-BAC and C16-BAC were 0.3, 0.5, 0.5 and 0.5 mg/L, respectively. The limits of quantification (LOQ, S/N =10) were 1.0, 1.5, 1.5, and 1.5 mg/L, respectively. The corrected peak area and the mass concentration of the four components mentioned above showed good linear relationships within the ranges of 1.0 - 400mg/L, 1.5 - 200mg/L, 1.5 -200mg/L and 1.5 -200mg/L, with linear correlation coefficients (Υ) of 0.999 5,0.999 8, 0.999 7 and 0.999 8, respectively. The established method was used for the determination of the four disinfectants in the compound chemical disinfectants. The results were in good agreement with those obtained by the high performance liquid chromatographic method. Source

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