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Beijing, China

Zhang Y.,Beijing Botanical Gardens | Lee Y.-I.,CSIC - National Museum of Natural Sciences | Deng L.,Beijing Botanical Gardens | Zhao S.,Beijing Botanical Gardens
Scientia Horticulturae | Year: 2013

Cypripedium macranthos is a lady's slipper orchid with high horticultural value. We document a reproducible protocol for asymbiotic seed germination based on a defined time frame. The optimum germination percentage was obtained from immature seeds collected at 6 weeks after pollination (WAP). At this time, the embryo reached the early globular stage. As seeds matured, the germination percentage declined sharply. Histological observation revealed that two distinct layers of seed coats enveloped the embryos tightly at maturity. The histochemical staining by Nile red and TBO indicated the presence of hydrophobic substance and phenolic compounds (e.g. lignin and cuticular material) in the seed coats. These features reflected the strong hydrophobic nature of seed coat that may cause the seed dormancy. Among the surveyed media, the modified Harvais medium was the optimum for germination. Among the surveyed organic supplements, potato homogenate (50gl-1) or banana homogenate (25 or 50gl-1) was suitable for the growth of seedlings. © 2013 Elsevier B.V. Source

Zhang Y.,Beijing Botanical Gardens | Zhang Y.P.,Beijing Botanical Gardens | Zhang Y.P.,Sichuan Agricultural University | Wang M.M.,Beijing Botanical Gardens | Zhao S.W.,Beijing Botanical Gardens
Acta Horticulturae | Year: 2013

The genus of Cypripedium, belonging to the subfamily of Cypripedioideae in Orchidaceae, had been recognized as a conservative hot spot and a -flagship- group in the world for its rich diversities, high ornamental value and endangered condition. The species of Cypripedium macranthos Sw. is the most beautiful species among its family in northern China. Therefore, in order to understand the real survival situation and conserve ex situ and in situ of this species, investigations of germplasm resources were implemented in 8 provinces in north and northeast China. Totally, 24 natural populations of C. macranthos were found at altitudes ranging from 2205 m in the most south part to 143 m in the most north part of the investigated areas. There were also C. guttatum Sw., C. calceolus L. and C. shanxience S.F. Chen growing in the habitat, with their varieties and natural hybrids forming a rich variation group. Most populations had been greatly over-collected, and most individuals of Cypripedium scattered in sub-alp meadow or under birch canopy with good light condition. The soil is fertile and slightly acidic with rich humus. In conclusion, wild Cypripedium resource growing in nature habitat had been over exploited and should be protected urgently. Source

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