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PubMed | Beijing Animal Husbandry Station and China Agricultural University
Type: | Journal: Scientific reports | Year: 2016

The egg-laying rates of hens approximately 470 days of age exhibited a positive correlation to blood melatonin levels. The hens with an egg-laying rate <30%, 30~90% and 90% had blood melatonin levels of 5.82.6, 74.032.9 and 445.9115.3ng/ml, respectively. When 10mg of melatonin was implanted into the hens at 300, 360, 470 and 550 days of age, the egg-laying rates increased 4.630.46%, 8.381.45%, 4.930.85% and 7.930.91%, respectively, compared to that of the controls. Melatonin implantation in hens at 300-470 days of age was observed to enhance egg production and reduce the rate of appearance of sharpei eggs. Melatonin (10mg) implanted in hens 360 days of age did not influence the blood levels of progesterone (P4) or the gene expression levels of ovarian follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), oestradiol receptor alpha (ER), superoxide dismutase 2 (SOD2) or melatonin receptor 1 (MT1). In contrast, melatonin significantly elevated the serum oestradiol-17 (E2) content, down-regulated the gene expression of gonadotropin-inhibitory hormone receptor (GnIHR), and enhanced the expression of melatonin receptor 2 (MT2). This result indicates that the improved egg-laying rate by melatonin was the result of increased serum oestradiol and decreased ovarian GnIHR. These alterations may be mediated by MT2 activation.

Jinlong L.,Beijing Jiaotong University | Shaoqing T.,Beijing Animal Husbandry Station | Zhiyuan Z.,Beijing Jiaotong University | Haichao W.,Beijing Jiaotong University | Qing X.,Beijing Jiaotong University
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2014

The DNA methylated states in muscle tissues from Beijing You chicken were analyzed using methylation sensitive amplified polymorphism (MSAP) combined with fluorescence labeling and capillary electrophoresis technology. We optimized the MSAP experimental condition including the genomic DNA concentration, dilution of pre-amplification prod-uct, the internal standard content and the concentrations of selective primers, Mg2+ and dNTPs. Repeated experiments showed that this method can automatically detect the global DNA methylation states in Beijing You chicken, and can be extended to other animals or plants with complex genomes and rich methylation polymorphism.

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