Beijing Amber Embryo Technology Co.

Beijing, China

Beijing Amber Embryo Technology Co.

Beijing, China

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Tang K.-Q.,CAS Shenyang Institute of Metal Research | Tang K.-Q.,Huazhong Agricultural University | Yang W.-C.,CAS Shenyang Institute of Metal Research | Yang W.-C.,Huazhong Agricultural University | And 6 more authors.
Molecular Biology Reports | Year: 2013

Progesterone receptor (PGR) and estrogen receptor alpha (ESRα), which mediate the biological effects of the steroid hormones progesterone and estrogen, play a central role in the establishment and maintenance of pregnancy. The objectives of this study were to detect bovine PGR and ESRα genes polymorphisms and analyze their relationships with the pregnancy rates after embryo transfer and the hormone concentrations at the day of embryo transfer. One reported SNP of PGR G59752C and a novel SNP of ESRα G75935C were analyzed in 132 recipients of Luxi cattle. For the PGR gene, recipients with g.59752 GG and g.59752 GC genotypes had obviously higher pregnancy rates than g.59752 CC genotype. For the ESRα gene, recipients with g.75935 GC and g.75935 CC genotypes had obviously higher pregnancy rates than g.75935 GG genotype. Furthermore, the same tendency was observed for these two genes that the same genotype groups with high pregnancy rates had high progesterone concentration and low estrogen concentration at the day of embryo transfer. These results showed for the first time that PGR G59752C and ESRα G75935C polymorphisms had obvious effects on the pregnancy rates after embryo transfer, and indicated that PGR G59752C and ESRα G75935C polymorphisms could be potential markers for recipient selection of embryo transfer. © 2012 Springer Science+Business Media Dordrecht.


Yang W.-C.,Northwest University, China | Yang L.-G.,Huazhong Agricultural University | Riaz H.,Huazhong Agricultural University | Tang K.-Q.,Huazhong Agricultural University | And 2 more authors.
Animal Reproduction Science | Year: 2013

The insulin-like growth factor 1 receptor (IGF1R) is a membrane glycoprotein mediating most biological actions of IGF1 and IGF2, and has an important effect on ovulation, pre-implantation embryo development and pregnancy rate. The objectives of this study were to detect IGF1R gene polymorphisms of cattle and analyze the relationship with superovulation performance and pregnancy rates after embryo transfer (ET), as well as the hormone concentrations at the day of ET. One reported SNP of IGF1R G404T and a novel SNP of IGF1R G399A were analyzed in 170 Chinese Holstein donor cows and 118 Luxi recipients cattle. Statistical analysis revealed that the G404T mutation was associated (p= 0.019) with increased ovulation rate and females with this mutation had enhanced performance in producing transferable embryos. For the polymorphic locus G399A, recipients with g.399 GG and g.399 GA genotypes had greater pregnancy rates after ET than that of g.399 AA genotype. Furthermore, the same tendency was observed that the genotype groups with greater pregnancy rates had greater progesterone and lesser estrogen concentrations, but these did not reach statistical significance. Results of the present study showed, for the first time, that the polymorphism in IGF1R is associated with superovulation traits, and indicated that the IGFIR gene can be used as a potential marker for donor selection. © 2013 Elsevier B.V.


Yang W.C.,Northwest University, China | Yang W.C.,Huazhong Agricultural University | Li S.J.,Beijing Amber Embryo Technology Co. | Chen L.,Beijing Amber Embryo Technology Co. | Yang L.G.,Huazhong Agricultural University
Genetics and Molecular Research | Year: 2014

Inhibin is a major regulator of secretion of follicle-stimulating hormone, which is involved in follicular development and regulation of steroidogenesis in females. The objectives of this study were to detect polymorphisms of the bovine inhibin beta-A subunit (INHβA) gene and to evaluate its associations with superovulatory responses in 171 Chinese Holstein cows treated for superovulation. Polymerase chain reaction-restricted fragment length polymorphism revealed a C>T transition determining the StyI polymorphism at position 7639 in intron I of the bovine INHβA gene, and three genotypes (CC, CT, and TT) were detected. The frequencies of the three genotypes showed a tendency for CT > TT > CC, and this polymorphism was in Hardy-Weinberg equilibrium. Statistical analysis revealed no significant differences of least square means for superovulation traits among the three genotypes (P > 0.05). These results demonstrate, for the first time, that the detected loci of the INHβA gene have no significant effects on superovulation performance in Chinese Holstein cows. © FUNPEC-RP.


Tang K.Q.,Huazhong Agricultural University | Yang W.C.,Huazhong Agricultural University | Yang W.C.,Northwest University, China | Li S.J.,Beijing Amber Embryo Technology Co. | Yang L.-G.,Huazhong Agricultural University
Genetics and Molecular Research | Year: 2013

Growth differentiation factor 9 (GDF9) belongs to the transforming growth factor β superfamily and plays a critical role in ovarian follicular development and ovulation rate. We examined the bovine GDF9 gene polymorphism and analyzed its association with superovulation performance. Based on the sequence of the bovine GDF9 gene, six pairs of primers were designed to detect single nucleotide polymorphisms of two exons and intron 1 of GDF9 using polymerase chain reaction-single-strand conformation polymorphism. Only the products amplified by primer 3-1 displayed polymorphisms. Sequencing revealed two mutations of A485T and A625T in intron 1 of the GDF9 gene in 171 Chinese Holstein cows treated for superovulation. Association analysis showed that these two single nucleotide polymorphisms of A485T and A625T had significant effects on the number of transferable embryos (P < 0.05), and the A625T polymorphism was significantly associated with the total number of ova (P < 0.05). In addition, a significant additive effect on the number of transferable embryos was detected in polymorphisms of A485T (P < 0.05). This study is the first to identify two polymorphisms in bovine GDF9 and describe their correlation with superovulation traits in Chinese Holstein cows. © FUNPEC-RP.


Tang K.-Q.,Huazhong Agricultural University | Li S.-J.,Beijing Amber Embryo Technology Co. | Yang W.-C.,Huazhong Agricultural University | Yu J.-N.,Huazhong Agricultural University | And 3 more authors.
Molecular Biology Reports | Year: 2011

To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, the PCR-RFLP method was applied to detect an A>G transition determining an MspI polymorphism at position 192 in the exon I of the bovine inhibin alpha (INHA) gene and evaluate its associations with superovulatory response in 118 Chinese Holstein cows treated for superovulation. Association analysis showed that cows with the GG genotype resulted in a significant increase in the number of ova (TNO) than AG and AA genotypes in the first (P = 0.023), second (P = 0.004) and third (P = 0.002) superovulation treatments and produced more transferable embryos (NTE) than that of AG and AA genotypes in the third (P = 0.045) superovulation treatment. Moreover, individuals with GG genotype produced more transferable embryos than AA (P < 0.05) genotype in the second superovulation treatment and all cows without superovulation response were mutations with genotypes of AA and AG. These results indicate that INHA gene can be used as a predictor for superovulation in Chinese Holstein cows, and imply that cows with AA genotype should be excluded for superovulation practices. © 2010 Springer Science+Business Media B.V.


Yang W.-C.,Huazhong Agricultural University | Li S.-J.,Beijing Amber Embryo Technology Co. | Tang K.-Q.,Huazhong Agricultural University | Hua G.-H.,Huazhong Agricultural University | And 4 more authors.
Animal Reproduction Science | Year: 2010

To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, variation in the bovine follicle stimulating hormone receptor (FSHR) gene was investigated using PCR-single-strand conformational (PCR-SSCP) and DNA sequencing. One single nucleotide polymorphism (SNP) of G-278A located in the 5′ upstream region of bovine FSHR gene was found in 118 Chinese Holstein cows treated for superovulation. Two SNPs of G-278A (GU253337) and A-320T (rs43676359) were analyzed. In polymorphic locus -278, all cows without superovulation response were mutations with genotypes of CD and DD. Cows with CC genotype had a significant increase in the total number of ova (TNO) (P < 0.01), and produced more transferable embryos (NTE) than those of the CD and DD genotypes (P < 0.01). At this locus, the additive effects seemed to be highly significant (P < 0.01) and allele C was associated with an increase in the TNO and NTE. These results indicated that the FSHR is a potential marker for superovulation response and can be used as a predictor for superovulation in Chinese Holstein cows. © 2010 Elsevier B.V. All rights reserved.


Yang W.-C.,Huazhong Agricultural University | Li S.-J.,Beijing Amber Embryo Technology Co. | Xie Y.-H.,Hubei well bred stock farm | Tang K.-Q.,Huazhong Agricultural University | And 3 more authors.
Livestock Science | Year: 2011

The present study attempts to explore a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, variation in the bovine type I gonadotropin releasing hormone receptor (GnRHRI) gene was investigated using PCR-single-strand conformational (PCR-SSCP) and DNA sequencing. Two single nucleotide polymorphisms (SNPs) of G286A and T340C were found in exon I of the bovine GnRHRI gene and evaluated its associations with superovulatory response in 104 Chinese Holstein cows. In polymorphic locus 286, all cows without superovulation response were mutations with genotype of GG. Individuals with GA genotype had a significant increase in total number of ova (TNO) (P< 0.01), and produced more transferable embryos (NTE) than those with GG genotype (P< 0.05). These results indicate that GnRHRI gene is a potential marker for superovulation response and can be used to predict the superovulatory response in Chinese Holstein cows. © 2010 Elsevier B.V.


Tang K.-Q.,Huazhong Agricultural University | Li S.-J.,Beijing Amber Embryo Technology Co. | Yang W.-C.,Huazhong Agricultural University | Yu J.-N.,Huazhong Agricultural University | And 3 more authors.
Molecular Biology Reports | Year: 2010

To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, the PCR-RFLP method was applied to detect an A>G transition determining an MspI polymorphism at position 192 in the exon I of the bovine inhibin alpha (INHA) gene and evaluate its associations with superovulatory response in 118 Chinese Holstein cows treated for superovulation. Association analysis showed that cows with the GG genotype resulted in a significant increase in the number of ova (TNO) than AG and AA genotypes in the first (P = 0.023), second (P = 0.004) and third (P = 0.002) superovulation treatments and produced more transferable embryos (NTE) than that of AG and AA genotypes in the third (P = 0.045) superovulation treatment. Moreover, individuals with GG genotype produced more transferable embryos than AA (P < 0.05) genotype in the second superovulation treatment and all cows without superovulation response were mutations with genotypes of AA and AG. These results indicate that INHA gene can be used as a predictor for superovulation in Chinese Holstein cows, and imply that cows with AA genotype should be excluded for superovulation practices. © 2010 Springer Science+Business Media B.V.


Yang W.C.,Huazhong Agricultural University | Tang K.Q.,Huazhong Agricultural University | Li S.J.,Beijing Amber Embryo Technology Co. | Yang L.G.,Huazhong Agricultural University
Reproduction in Domestic Animals | Year: 2011

The objective of this study was to identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism. The PCR-RFLP method was applied to detect two reported single nucleotide polymorphisms (SNPs) of G59752C and T81637C (rs41614030) located in introns 3 and 4 of the bovine progesterone receptor (PGR) gene in 171 Chinese Holstein cows treated for superovulation and evaluate its associations with superovulation traits. In polymorphic locus 81637, all cows without superovulation response were g.81637TC and g.81637TT genotypes. Association analysis showed that these two SNPs had significant effects on the total number of ova (TNO) (p<0.05), and the T81637C polymorphism was significantly associated with the number of transferable embryos (p<0.05). In addition, significant additive effects (p<0.05) on TNO were detected in the polymorphisms of G59752C and T81637C. These results showed for the first time that the G59752C and T81637C polymorphisms in PGR gene were associated with superovulation traits and indicated that PGR gene can be used as a predictor for superovulation in Chinese Holstein cows. © 2011 Blackwell Verlag GmbH.


PubMed | Beijing Amber Embryo Technology Co., Northwest Agriculture and Forestry University and Huazhong Agricultural University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2014

Inhibin is a major regulator of secretion of follicle-stimulating hormone, which is involved in follicular development and regulation of steroidogenesis in females. The objectives of this study were to detect polymorphisms of the bovine inhibin beta-A subunit (INHA) gene and to evaluate its associations with superovulatory responses in 171 Chinese Holstein cows treated for superovulation. Polymerase chain reaction-restricted fragment length polymorphism revealed a C>T transition determining the StyI polymorphism at position 7639 in intron I of the bovine INHA gene, and three genotypes (CC, CT, and TT) were detected. The frequencies of the three genotypes showed a tendency for CT > TT > CC, and this polymorphism was in Hardy-Weinberg equilibrium. Statistical analysis revealed no significant differences of least square means for superovulation traits among the three genotypes (P > 0.05). These results demonstrate, for the first time, that the detected loci of the INHA gene have no significant effects on superovulation performance in Chinese Holstein cows.

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