Entity

Time filter

Source Type


Tang K.-Q.,Huazhong Agricultural University | Li S.-J.,Beijing Amber Embryo Technology Co. | Yang W.-C.,Huazhong Agricultural University | Yu J.-N.,Huazhong Agricultural University | And 3 more authors.
Molecular Biology Reports | Year: 2011

To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, the PCR-RFLP method was applied to detect an A>G transition determining an MspI polymorphism at position 192 in the exon I of the bovine inhibin alpha (INHA) gene and evaluate its associations with superovulatory response in 118 Chinese Holstein cows treated for superovulation. Association analysis showed that cows with the GG genotype resulted in a significant increase in the number of ova (TNO) than AG and AA genotypes in the first (P = 0.023), second (P = 0.004) and third (P = 0.002) superovulation treatments and produced more transferable embryos (NTE) than that of AG and AA genotypes in the third (P = 0.045) superovulation treatment. Moreover, individuals with GG genotype produced more transferable embryos than AA (P < 0.05) genotype in the second superovulation treatment and all cows without superovulation response were mutations with genotypes of AA and AG. These results indicate that INHA gene can be used as a predictor for superovulation in Chinese Holstein cows, and imply that cows with AA genotype should be excluded for superovulation practices. © 2010 Springer Science+Business Media B.V. Source


Yang W.-C.,Huazhong Agricultural University | Li S.-J.,Beijing Amber Embryo Technology Co. | Xie Y.-H.,Hubei well bred stock farm | Tang K.-Q.,Huazhong Agricultural University | And 3 more authors.
Livestock Science | Year: 2011

The present study attempts to explore a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, variation in the bovine type I gonadotropin releasing hormone receptor (GnRHRI) gene was investigated using PCR-single-strand conformational (PCR-SSCP) and DNA sequencing. Two single nucleotide polymorphisms (SNPs) of G286A and T340C were found in exon I of the bovine GnRHRI gene and evaluated its associations with superovulatory response in 104 Chinese Holstein cows. In polymorphic locus 286, all cows without superovulation response were mutations with genotype of GG. Individuals with GA genotype had a significant increase in total number of ova (TNO) (P< 0.01), and produced more transferable embryos (NTE) than those with GG genotype (P< 0.05). These results indicate that GnRHRI gene is a potential marker for superovulation response and can be used to predict the superovulatory response in Chinese Holstein cows. © 2010 Elsevier B.V. Source


Tang K.-Q.,Huazhong Agricultural University | Li S.-J.,Beijing Amber Embryo Technology Co. | Yang W.-C.,Huazhong Agricultural University | Yu J.-N.,Huazhong Agricultural University | And 3 more authors.
Molecular Biology Reports | Year: 2010

To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, the PCR-RFLP method was applied to detect an A>G transition determining an MspI polymorphism at position 192 in the exon I of the bovine inhibin alpha (INHA) gene and evaluate its associations with superovulatory response in 118 Chinese Holstein cows treated for superovulation. Association analysis showed that cows with the GG genotype resulted in a significant increase in the number of ova (TNO) than AG and AA genotypes in the first (P = 0.023), second (P = 0.004) and third (P = 0.002) superovulation treatments and produced more transferable embryos (NTE) than that of AG and AA genotypes in the third (P = 0.045) superovulation treatment. Moreover, individuals with GG genotype produced more transferable embryos than AA (P < 0.05) genotype in the second superovulation treatment and all cows without superovulation response were mutations with genotypes of AA and AG. These results indicate that INHA gene can be used as a predictor for superovulation in Chinese Holstein cows, and imply that cows with AA genotype should be excluded for superovulation practices. © 2010 Springer Science+Business Media B.V. Source


Tang K.Q.,Huazhong Agricultural University | Yang W.C.,Huazhong Agricultural University | Yang W.C.,Northwest University, China | Li S.J.,Beijing Amber Embryo Technology Co. | Yang L.-G.,Huazhong Agricultural University
Genetics and Molecular Research | Year: 2013

Growth differentiation factor 9 (GDF9) belongs to the transforming growth factor β superfamily and plays a critical role in ovarian follicular development and ovulation rate. We examined the bovine GDF9 gene polymorphism and analyzed its association with superovulation performance. Based on the sequence of the bovine GDF9 gene, six pairs of primers were designed to detect single nucleotide polymorphisms of two exons and intron 1 of GDF9 using polymerase chain reaction-single-strand conformation polymorphism. Only the products amplified by primer 3-1 displayed polymorphisms. Sequencing revealed two mutations of A485T and A625T in intron 1 of the GDF9 gene in 171 Chinese Holstein cows treated for superovulation. Association analysis showed that these two single nucleotide polymorphisms of A485T and A625T had significant effects on the number of transferable embryos (P < 0.05), and the A625T polymorphism was significantly associated with the total number of ova (P < 0.05). In addition, a significant additive effect on the number of transferable embryos was detected in polymorphisms of A485T (P < 0.05). This study is the first to identify two polymorphisms in bovine GDF9 and describe their correlation with superovulation traits in Chinese Holstein cows. © FUNPEC-RP. Source


Yang W.-C.,Huazhong Agricultural University | Li S.-J.,Beijing Amber Embryo Technology Co. | Tang K.-Q.,Huazhong Agricultural University | Hua G.-H.,Huazhong Agricultural University | And 4 more authors.
Animal Reproduction Science | Year: 2010

To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, variation in the bovine follicle stimulating hormone receptor (FSHR) gene was investigated using PCR-single-strand conformational (PCR-SSCP) and DNA sequencing. One single nucleotide polymorphism (SNP) of G-278A located in the 5′ upstream region of bovine FSHR gene was found in 118 Chinese Holstein cows treated for superovulation. Two SNPs of G-278A (GU253337) and A-320T (rs43676359) were analyzed. In polymorphic locus -278, all cows without superovulation response were mutations with genotypes of CD and DD. Cows with CC genotype had a significant increase in the total number of ova (TNO) (P < 0.01), and produced more transferable embryos (NTE) than those of the CD and DD genotypes (P < 0.01). At this locus, the additive effects seemed to be highly significant (P < 0.01) and allele C was associated with an increase in the TNO and NTE. These results indicated that the FSHR is a potential marker for superovulation response and can be used as a predictor for superovulation in Chinese Holstein cows. © 2010 Elsevier B.V. All rights reserved. Source

Discover hidden collaborations