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Yang M.M.,Northwest Agriculture and Forestry University | Yang M.M.,Chinese Academy of Forestry | Zhang Y.A.,Chinese Academy of Forestry | Wang Q.H.,Chinese Academy of Forestry | And 6 more authors.
Biocontrol Science and Technology | Year: 2012

Dendrolimus kikuchii Matsumura nucleopolyhedrovirus (DekiNPV) has been considered as a biological control agent against D. kikuchii. We examined the infectivity and replication of DekiNPV in 11 other lepidopteran species, including Dendrolimus houi (Lajonquiere), Dendrolimus punctatus (Walker), D. punctatus wenshanensis (Tsai et Liu), Dendrolimus spectabilis (Butler), Hyphantria cunea (Drury), Lymantria dispar (L.), Ectropis grisescens (Warren), Helicoverpa armigera (Hübner), Spodoptera exigua (Hübner), Plutella xylostella (L.) and Spodoptera litura (Fabricius) larvae to define the host range of DekiNPV and identify suitable alternate hosts. Our study showed that DekiNPV is highly specific to D. kikuchii, but triggers covert infections in H. cunea, D. houi, D. punctatus, D. punctatus wenshanensis and D. spectabilis larvae into overt symptoms, indicating that DekiNPV is suitable as an ideal biocontrol agent for D. kikuchii, D. houi, D. punctatus, D. punctatus wenshanensis, D. spectabilis and H. cunea. © 2012 Copyright Taylor and Francis Group, LLC. Source


Yang M.M.,Northwest Agriculture and Forestry University | Yang M.M.,Chinese Academy of Forestry | Zhang Y.A.,Chinese Academy of Forestry | Wang Q.H.,Chinese Academy of Forestry | And 6 more authors.
Biocontrol Science and Technology | Year: 2012

A polymerase chain reaction (PCR) assay was developed with a pair of primers, designed based on the unique open reading frame 146 of Dendrolimus kikuchii nucleopolyhedrovirus (DekiNPV). DekiNPV DNA served as a template to amplify 426 bp and 655 bp segments. Results from the sequenced PCR products were consistent with the size of selected primer segments. Two primers were applied to assay DekiNPV DNA and detect intact DekiNPV occlusion bodies (OBs), showing a minimum detection of 0.8 fg/mL DNA and 5 OBs/mL, respectively, indicating the existence of DekiNPV in the infected insects. The assay is characterised with high sensitivity, remarkable specificity and early assay, demonstrating its potential for applications in the studies of DekiNPV host range, substitution of DekiNPV hosts, dynamic DekiNPV changes in D. kikuchii population and routine molecular diagnostic procedures. © 2012 Copyright Taylor and Francis Group, LLC. Source

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