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Fan H.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | Liu X.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | Hong H.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | Shen S.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | And 4 more authors.
Journal of Food Protection

Postmortem quality changes of black carp (Mylopharyngodon piceus) fillets stored at 20, 4, and 08C (in ice) were determined in terms of pH value, K value, total volatile basic nitrogen, free amino acids, biogenic amines, drip loss, electrical conductivity (EC), sensory score, and microbial growth. The results showed that black carp fillets could maintain a good quality for 2, 9, and 12 days when stored at 20, 4, and 08C, respectively. Pseudomonads, Aeromonas, and Enterobacteriaceae were the main spoilage bacteria in black carp. Tryptamine, 2-phenylethylamine, putrescine, cadaverine, and tyramine increased significantly (P , 0.05) during storage at the three temperatures, but not spermidine and spermine, among which tyramine and putrescine were the main biogenic amines in black carp fillets. A significantly higher concentration of histamine (132.05 mg/kg on the third day) was detected in the samples stored at 208C (P , 0.01) than at 4 and 08C (0.62 to 3.28 mg/kg) throughout storage, indicating storage of samples at 208C favored the formation of histamine. The accumulations of tyramine, cadaverine, and histamine were highly correlated with the productions of tyrosine, lysine, and histidine, respectively. Correlations between EC and sensory, physical, chemical, and microbial parameters at the three storage temperatures showed that EC could be used as a better quality indicator to assess the overall quality of fish stored at 4 and 08C (low temperature) than at 208C. © Copyright International Association for Food Protection. Source

Zhang N.,China Agricultural University | Chen H.,Beijing Technology and Business University | Sun B.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | Mao X.,China Agricultural University | And 2 more authors.
International Journal of Molecular Sciences

To compare the volatile compounds of Chinese black truffle and white truffle from Yunnan province, this study presents the application of a direct solvent extraction/solvent-assisted flavor evaporation (DSE-SAFE) coupled with a comprehensive two-dimensional gas chromatography (GC × GC) high resolution time-of-flight mass spectrometry (HR-TOF/MS) and an electronic nose. Both of the analytical methods could distinguish the aroma profile of the two samples. In terms of the overall profile of truffle samples in this research, more kinds of acids were detected via the method of DSE-SAFE. Besides, compounds identified in black truffle (BT), but not in white truffle (WT), or vice versa, and those detected in both samples at different levels were considered to play an important role in differentiating the two samples. According to the analysis of electronic nose, the two samples could be separated, as well. © 2016 by the authors; licensee MDPI, Basel, Switzerland. Source

Liu F.,Huazhong Agricultural University | Liu F.,China Agricultural University | Liao X.,China Agricultural University | Liao X.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | Wang Y.,China Agricultural University
Food and Bioprocess Technology

The effects of high-pressure processing (HPP 300 MPa/15 min, 400 MPa/5 min, 500 MPa/2.5 min, and 600 MPa/1 min) and high-temperature/short-time processing (HTST 110 °C/8.6 s), with or without blanching, on mango pulp were comparatively evaluated in terms of the antioxidant compounds, antioxidant capacity, sugars, and color. Blanching treatment significantly increased the total phenol content and the antioxidant capacity of mango pulp, but did not change the levels of L-ascorbic acid, carotenoids, sugars, and visual color (total color difference, △E < 2.00). Both HPP and HTST treatments significantly increased the total phenol content and antioxidant capacity of un-blanched mango pulp, but no significant changes occurred in the blanched mango pulp. HPP did not affect the levels of L-ascorbic acid, carotenoids, and sugars in mango pulp regardless of blanching. However, HTST significantly decreased the fructose and glucose levels, as well as induced the isomerization of β-carotene, with the increase in 13-cis-β-carotene accompanied by the decrease in all-trans-β-carotene. Moreover, HPP-treated mango pulp consistently showed lower △E values than those HTST-treated samples, regardless of blanching. © 2016 Springer Science+Business Media New York Source

Ge S.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | Zhang M.,Beijing Technology and Business University | Zhang M.,China Agricultural University | Hou C.,China Agricultural University | And 6 more authors.
Journal of Chinese Institute of Food Science and Technology

Quinoline compounds had become common environmental pollutants, various kind of quinolone compounds were genotoxic and have the potential to induce cancer. In this study, a HPLC method was used to determine the degradation of 4HAQO, a powerful carcinogenicquinoline compound, induced by the secretion of L. Salivarius Ren and helped in degenotoxic activity. By means of molecular weight cutting off and enzyme treatment, we indicated that, it was the H2O2 in the secretion of L. Salivarius Ren that played the essential role during 4HAQO degradation. © 2016, Chinese Institute of Food Science and Technology. All right reserved. Source

Chen L.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | Chen L.,China Agricultural University | Xu P.,CAS Research Center for Eco Environmental Sciences | Diao J.,China Agricultural University | And 5 more authors.
Journal of Hazardous Materials

Beta-cypermethrin (BCYP), a synthetic pyrethriod (PYR) pesticide which is a mixture of the alpha- and theta- cypermethrin, have been reported various toxicological profiles to non-target organisms. But little is known about assimilation, accumulation and toxic effects of BCYP in reptiles. The present study firstly elucidated absorption, tissue distribution, excretion of BCYP in Eremias argus Treated group were administered orally with BCYP 20 mg/kg body weight (bw) dissolved in corn oil. Neurotoxicity was observed at 24 h after gavage, and the poisoning symptom ameliorated at 72 h. The changes of BCYP concentration depended on degradation time and tissues. Lizards had a strong capacity to eliminate BCYP with different tissue distribution. The tissues concentration of BCYP from high to low were intestine, stomach, heart, kidney, blood, lung, liver and brain. Bimodal phenomena were observed in lung, liver and kidney. These results may be due to the activities of enzymes, circadian rhythm, and enterohepatic circulation in lizards. Based on the results of organ coefficient and histopathology analysis in liver, the liver was confirmed as the main target organ. © 2015 Elsevier B.V. Source

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