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Roman-Ponce B.,National Polytechnic Institute of Mexico | Zhang Y.J.,China Agricultural University | Vasquez-Murrieta M.S.,National Polytechnic Institute of Mexico | Sui X.H.,China Agricultural University | And 6 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2016

Two Gram-negative, aerobic, non-motile, rod-shaped bacterial strains, FH13Tand FH23, representing a novel group of Rhizobium isolated from root nodules of Phaseolus vulgaris in Mexico, were studied by a polyphasic analysis. Phylogeny of 16S rRNA gene sequences revealed them to be members of the genus Rhizobium related most closely to ‘Rhizobium anhuiense’ CCBAU 23252 (99.7% similarity), Rhizobium leguminosarum USDA 2370T(98.6%), and Rhizobium sophorae CCBAU 03386T and others (≤98.3%). In sequence analyses of the housekeeping genes recA, glnII and atpD, both strains formed a subclade distinct from all defined species of the genus Rhizobium at sequence similarities of 82.3-94.0%, demonstrating that they represented a novel genomic species in the genus Rhizobium. Mean levels of DNA-DNA relatedness between the reference strain FH13T and the type strains of related species varied between 13.0±2.0 and 52.1±1.2%. The DNA G+C content of strain FH13T was 63.5 mol% (Tm). The major cellular fatty acids were 16: 0, 17: 0 anteiso, 18: 0, summed feature 2 (12: 0 aldehyde/unknown 10.928) and summed feature 8 (18: ω7c). The fatty acid 17: ω5c was unique for this strain. Some phenotypic features, such as failure to utilize adonitol, L-arabinose, D-fructose and D-fucose, and ability to utilize D-galacturonic acid and itaconic acid as carbon source, could also be used to distinguish strain FH13T from the type strains of related species. Based upon these results, a novel species, Rhizobium acidisoli sp. nov., is proposed, with FH13T(=CCBAU 101094T=HAMBI 3626T=LMG 28672T) as the type strain. © 2015 IUMS. Source


Gao J.-L.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Lv F.-Y.,Chinese Academy of Agricultural Sciences | Wang X.-M.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Yuan M.,Chinese Academy of Agricultural Sciences | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A novel Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain 522T, was isolated from surface-sterilized root tissue of maize planted in Fangshan District of Beijing, China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belonged to the genus Flavobacterium and showed less than 93.9% similarity to the type strains of all recognized species of the genus Flavobacterium. The predominant respiratory quinone was menaquinone-6 and the polar lipid profile was composed of the major lipids phosphatidylethanolamine, phosphatidylserine and two unidentified amino lipids. The major fatty acids were C15:0, iso-C15:0, iso-C15:1 G and iso-C16:0.The G+C content of the DNA was 37.7 mol%. The results of physiological and biochemical tests and the differences in fatty acid profiles allowed the clear phenotypic differentiation of strain 522T from closely related species of the genus Flavobacterium. Strain 522T therefore represents a novel species within the genus Flavobacterium, for which the name Flavobacterium endophyticum sp. nov. is proposed. The type strain is 522T (=ACCC 19708T=DSM 29537T). © 2015 IUMS. Source


Gao J.-L.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Lv F.-Y.,Chinese Academy of Agricultural Sciences | Wang X.-M.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Qiu T.-L.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | And 6 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2015

A novel Gram-positive, aerobic, motile, endospore-forming, rod-shaped bacterium, designated 373T was isolated from surface-sterilised root tissue of a maize planted in Fangshan District of Beijing, Peopole’s Republic of China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belongs to the genus Paenibacillus. The highest 16S rRNA gene sequence similarity was found between strain 373T and Paenibacillus hunanensis (98.1 %), meanwhile the 16S rRNA gene sequence similarity between strain 373T and the type strains of other recognised members of the genus Paenibacillus were all below 95.6 %. However, the DNA–DNA hybridization values between strain 373T and the type strain P. hunanensis DSM 22170T was 30.2 %. The DNA G+C content of strain 373T was determined to be 46.0 mol%. The predominant respiratory quinone was identified as menaquinone-7 and the polar lipid profile was found to be composed of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were found to consist of anteiso-C15: 0 (59.6 %), anteiso-C17: 0 (12.8 %) and C16: 0 (6.7 %). The results of physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 373T from the closely related species in this genus Paenibacillus. Strain 373T is concluded to represent a novel species within the genus Paenibacillus, for which the name Paenibacillus wenxiniae sp. nov. is proposed, with the type strain 373T (= CGMCC 1.15007 T = DSM100576). © 2015, Springer International Publishing Switzerland. Source

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