Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology

Beijing, China

Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology

Beijing, China
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Gao J.-L.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Sun P.,Chinese Academy of Forestry | Mao X.-J.,Chinese Academy of Agricultural Sciences | Du Y.-L.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2017

A novel Gram-staining-negative, aerobic, non-motile by gliding and rod-shaped strain, designated 22T, was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, PR China. The highest levels of 16S rRNA gene sequence similarity were found with respect to Pedobacter suwonensis 15-52T (97.5%), Pedobacter terrae DS-57T (97.1%) and Pedobacter alluvionis NWER-II11T (97.0%). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain 22T is a member of the genus Pedobacter. The isolate Exhibited relatively low levels of DNA–DNA relatedness with respect to P. suwonensis DSM 18130T (21.3±2.0%), P. alluvionis DSM 19624T (38.1±1.8%) and P. terrae DSM 17933T (17.1±1.4%). The DNA G+C content was 41.2±0.5 mol%. The major isoprenoid quinone was menaquinone-7 (MK-7). The major component in the polyamine pattern was sym-homospermidine. The major polar lipids consisted of phosphatidylethanolamine, three unidentified aminolipids and one unidentified lipid. The major fatty acids were identified as iso-C15:0 and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The results of the physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 22T from the related species with high 16S rRNA gene sequence similarity, P. suwonensis DSM 18130T, P. alluvionis DSM 19624T and P. terrae DSM 17933T. Strain 22T represents a novel species within the genus Pedobacter, for which the name Pedobacter zeae sp. nov. is proposed, with the type strain 22T (=CGMCC 1.15287T=DSM 100774T). © 2017 IUMS.


Gao J.-L.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Sun P.,Chinese Academy of Forestry | Wang X.-M.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Lv F.-Y.,Chinese Academy of Agricultural Sciences | Sun J.-G.,Chinese Academy of Agricultural Sciences
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2017

A novel Gram-stain positive, aerobic, non-motile, non-spore-forming and rod-shaped strain designated 1204T was isolated from surface-sterilised stem tissue of maize planted in Fangshan District of Beijing, People’s Republic of China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belongs to the genus Microbacterium. High levels of 16S rRNA gene sequence similarity were found between strain 1204T and Microbacterium enclense NIO-1002T (98.8%) and Microbacterium proteolyticum RZ36T (98.4%) respectively. However, the DNA–DNA hybridization values between strain 1204T and its closely related species M. proteolyticum DSM 27100T and M. enclense DSM 25125T were 53.9 ± 1.6 and 20.9 ± 1.5% respectively. The DNA G+C content of strain 1204T was determined to be 68.0 mol%. The major fatty acids were found to consist of anteiso-C15:0 (37.6%), iso-C16:0 (28.6%) and anteiso-C17:0 (16.6%). The predominant menaquinone was MK-11 and the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid. The results of physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 1204T from the closely related species in the genus Microbacterium. Thus, it was concluded that strain 1204T represents a novel species within the genus Microbacterium, for which the name Microbacterium zeae sp. nov. is proposed, with the type strain 1204T (= CGMCC 1.15289 = DSM 100750). © 2017 Springer International Publishing Switzerland


Roman-Ponce B.,National Polytechnic Institute of Mexico | Zhang Y.J.,China Agricultural University | Vasquez-Murrieta M.S.,National Polytechnic Institute of Mexico | Sui X.H.,China Agricultural University | And 6 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2016

Two Gram-negative, aerobic, non-motile, rod-shaped bacterial strains, FH13Tand FH23, representing a novel group of Rhizobium isolated from root nodules of Phaseolus vulgaris in Mexico, were studied by a polyphasic analysis. Phylogeny of 16S rRNA gene sequences revealed them to be members of the genus Rhizobium related most closely to ‘Rhizobium anhuiense’ CCBAU 23252 (99.7% similarity), Rhizobium leguminosarum USDA 2370T(98.6%), and Rhizobium sophorae CCBAU 03386T and others (≤98.3%). In sequence analyses of the housekeeping genes recA, glnII and atpD, both strains formed a subclade distinct from all defined species of the genus Rhizobium at sequence similarities of 82.3-94.0%, demonstrating that they represented a novel genomic species in the genus Rhizobium. Mean levels of DNA-DNA relatedness between the reference strain FH13T and the type strains of related species varied between 13.0±2.0 and 52.1±1.2%. The DNA G+C content of strain FH13T was 63.5 mol% (Tm). The major cellular fatty acids were 16: 0, 17: 0 anteiso, 18: 0, summed feature 2 (12: 0 aldehyde/unknown 10.928) and summed feature 8 (18: ω7c). The fatty acid 17: ω5c was unique for this strain. Some phenotypic features, such as failure to utilize adonitol, L-arabinose, D-fructose and D-fucose, and ability to utilize D-galacturonic acid and itaconic acid as carbon source, could also be used to distinguish strain FH13T from the type strains of related species. Based upon these results, a novel species, Rhizobium acidisoli sp. nov., is proposed, with FH13T(=CCBAU 101094T=HAMBI 3626T=LMG 28672T) as the type strain. © 2015 IUMS.


PubMed | Chinese Academy of Sciences, Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, National Polytechnic Institute of Mexico, Northwest Agriculture and Forestry University and China Agricultural University
Type: Journal Article | Journal: International journal of systematic and evolutionary microbiology | Year: 2016

Two Gram-negative, aerobic, non-motile, rod-shaped bacterial strains, FH13T and FH23, representing a novel group of Rhizobium isolated from root nodules of Phaseolus vulgaris in Mexico, were studied by a polyphasic analysis. Phylogeny of 16S rRNA gene sequences revealed them to be members of the genus Rhizobium related most closely to Rhizobium anhuiense CCBAU 23252 (99.7% similarity), Rhizobium leguminosarum USDA 2370T (98.6%), and Rhizobium sophorae CCBAU 03386T and others (98.3%). In sequence analyses of the housekeeping genes recA, glnII and atpD, both strains formed a subclade distinct from all defined species of the genus Rhizobium at sequence similarities of 82.3-94.0%, demonstrating that they represented a novel genomic species in the genus Rhizobium. Mean levels of DNA-DNA relatedness between the reference strain FH13T and the type strains of related species varied between 13.02.0 and 52.11.2%. The DNA G+C content of strain FH13T was 63.5mol% (Tm). The major cellular fatty acids were 16:0, 17:0 anteiso, 18:0, summed feature 2 (12:0 aldehyde/unknown 10.928) and summed feature 8 (18:17c). The fatty acid 17:15c was unique for this strain. Some phenotypic features, such as failure to utilize adonitol, l-arabinose, d-fructose and d-fucose, and ability to utilize d-galacturonic acid and itaconic acid as carbon source, could also be used to distinguish strain FH13T from the type strains of related species. Based upon these results, a novel species, Rhizobium acidisoli sp. nov., is proposed, with FH13T (=CCBAU 101094T=HAMBI 3626T=LMG 28672T) as the type strain.


PubMed | Chinese Academy of Forestry, Chinese Academy of Agricultural Sciences and REPUBLIC RESOURCES
Type: | Journal: International journal of systematic and evolutionary microbiology | Year: 2016

A Gram-staining-positive, moderately halophilic, aerobic, endospore-forming, rod-shaped bacterial strain, designated WD4L-1T was isolated from surface-sterilized stem tissue of a poplar tree planted in the Wudalianchi National Geopark of Heilongjiang province, Peoples Republic of China. This new isolate grew in the presence of 0~15 % (w/v) NaCl, at pH 6.0 ~9.0 and 15~ 50 C; optimum growth was observed with 7~ 8 % (w/v) NaCl, at pH 7.0 and 30 C. The 16S rRNA gene sequence analysis indicated that the strain WD4L-1T belonged to the genus Lentibacillus, and was most closely related to the type strain SL-MJ1T of Lentibacillus garicola with the sequence similarity of 96.1%. The DNA G+C content of strain WD4L-1T was determined to be 36.9 mol %. The respiratory quinone was identified as menaquinone-7 (MK-7) and the major lipids were diphosphatidylglycerol and phosphatidylglycerol and one unidentified phospholipid. The major fatty acid of strain WD4L-1T were anteiso-C15: 0, iso-C15: 0 and anteiso-C17:0. The results of the physiological and biochemical tests and the minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain WD4L-1T from its closely related species Lentibacillus garicola JCM 30131T. Strain WD4L-1T represents a novel species within the genus Lentibacillus, for which the name Lentibacillus populi sp. nov. is proposed, with the type strain WD4L-1T ( = CGMCC 1.15454 T = DSM 101738 T ).


Gao J.-L.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Lv F.-Y.,Chinese Academy of Agricultural Sciences | Wang X.-M.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Yuan M.,Chinese Academy of Agricultural Sciences | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A novel Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain 522T, was isolated from surface-sterilized root tissue of maize planted in Fangshan District of Beijing, China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belonged to the genus Flavobacterium and showed less than 93.9% similarity to the type strains of all recognized species of the genus Flavobacterium. The predominant respiratory quinone was menaquinone-6 and the polar lipid profile was composed of the major lipids phosphatidylethanolamine, phosphatidylserine and two unidentified amino lipids. The major fatty acids were C15:0, iso-C15:0, iso-C15:1 G and iso-C16:0.The G+C content of the DNA was 37.7 mol%. The results of physiological and biochemical tests and the differences in fatty acid profiles allowed the clear phenotypic differentiation of strain 522T from closely related species of the genus Flavobacterium. Strain 522T therefore represents a novel species within the genus Flavobacterium, for which the name Flavobacterium endophyticum sp. nov. is proposed. The type strain is 522T (=ACCC 19708T=DSM 29537T). © 2015 IUMS.


Gao J.-L.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Lv F.-Y.,Chinese Academy of Agricultural Sciences | Wang X.-M.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | Qiu T.-L.,Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology | And 6 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2015

A novel Gram-positive, aerobic, motile, endospore-forming, rod-shaped bacterium, designated 373T was isolated from surface-sterilised root tissue of a maize planted in Fangshan District of Beijing, Peopole’s Republic of China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belongs to the genus Paenibacillus. The highest 16S rRNA gene sequence similarity was found between strain 373T and Paenibacillus hunanensis (98.1 %), meanwhile the 16S rRNA gene sequence similarity between strain 373T and the type strains of other recognised members of the genus Paenibacillus were all below 95.6 %. However, the DNA–DNA hybridization values between strain 373T and the type strain P. hunanensis DSM 22170T was 30.2 %. The DNA G+C content of strain 373T was determined to be 46.0 mol%. The predominant respiratory quinone was identified as menaquinone-7 and the polar lipid profile was found to be composed of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were found to consist of anteiso-C15: 0 (59.6 %), anteiso-C17: 0 (12.8 %) and C16: 0 (6.7 %). The results of physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 373T from the closely related species in this genus Paenibacillus. Strain 373T is concluded to represent a novel species within the genus Paenibacillus, for which the name Paenibacillus wenxiniae sp. nov. is proposed, with the type strain 373T (= CGMCC 1.15007 T = DSM100576). © 2015, Springer International Publishing Switzerland.


PubMed | Beijing Academy of Agriculture and Forestry Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Chinese Academy of Agricultural Sciences and Chinese Academy of Forestry
Type: Journal Article | Journal: International journal of systematic and evolutionary microbiology | Year: 2016

A novel Gram-stain-negative, aerobic and rod-shaped bacterial strain, designated 65T, was isolated from surface-sterilized root tissue of maize, collected from Fangshan District of Beijing, Peoples Republic of China, and was subjected to a taxonomic study by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 65T belonged to the genus Dyadobacter and had highest 16S rRNA gene sequence similarity to Dyadobacter jiangsuensis CGMCC 1.12969T (99.1 %), Dyadobacter beijingensis CGMCC 1.6375T (98.8%), Dyadobacter fermentans DSM 18053T (98.6%) and Dyadobacter soli KCTC 22481T (98.6%). However, the new isolate exhibited relatively low levels of DNA-DNA relatedness with respect to D. jiangsuensis CGMCC 1.12969T (18.21.3%), D. beijingensis CGMCC 1.6375T (14.22.0%), D. fermentans DSM 18053T (14.12.0%) and D. soli KCTC 22481T (13.80.6%). The predominant respiratory quinone was menaquinone-7 (MK-7) and the major cellular fatty acids were summed feature 3 (C16:17c and/or iso-C15:0 2-OH), iso-C15:0, iso-C17:0 3-OH, C16:15c, iso-C15:0 3-OH, C16:0 3-OH and C16:0. The polar lipid profile of strain 65T revealed the presence of phosphatidylethanolamine, four aminolipids and two unidentified phospholipids. The DNA G+C content was 46.6 mol%. The results of physiological and biochemical tests and the differences in the fatty acid profiles allowed the clear phenotypic differentiation of strain 65T from closely related species of the genus Dyadobacter. Strain 65T thus represents a novel species within the genus Dyadobacter, for which the name Dyadobacterendophyticus sp. nov. is proposed. The type strain is 65T (=CGMCC 1.15288T=DSM 100786T).

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