Beijing Academy Of Agri And Forestry Science

Beijing, China

Beijing Academy Of Agri And Forestry Science

Beijing, China
SEARCH FILTERS
Time filter
Source Type

Wang D.,Beijing Academy Of Agri And Forestry Science | Lei Y.,Beijing Academy Of Agri And Forestry Science | Lei Y.,Shihezi University | Ma Y.,Beijing Academy Of Agri And Forestry Science | And 2 more authors.
Journal of Functional Foods | Year: 2014

Ten anthocyanins were identified from purple corn (. Zea mays L.), Jingzi variety, by high-performance liquid chromatography tandem mass spectrometry in order to assess their anti-avian leukosis virus subgroup A (ALV-A) induced cell apoptosis in vitro. The inhibitory activity and mechanism were evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Trans AM™ and western blotting. Total anthocyanins from purple corn (TAPC) exhibited a dose-dependent effect on decreasing apoptosis of chicken embryo fibroblasts, DF-1 cell, infected with ALV-A in the range of 4-12μg/mL. Meanwhile, TAPC decreased transfer levels of nuclear transcription factor kappa B (NF-κB) p50/p65 from cytoplasm to nucleus, and downregulated the expression of NF-κB p50/p65 which promoted DF-1 cell cytopathic effects evaluated by western blotting. TAPC inhibited ALV-A induced apoptosis in DF-1 cells via inactivation of NF-κB p50/p65. © 2014 Elsevier Ltd.

Loading Beijing Academy Of Agri And Forestry Science collaborators
Loading Beijing Academy Of Agri And Forestry Science collaborators