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Shi P.,Chinese Academy of Agricultural Sciences | Du Y.,Chinese Academy of Agricultural Sciences | Yang H.,Chinese Academy of Agricultural Sciences | Huang H.,Chinese Academy of Agricultural Sciences | And 3 more authors.
BioMed Research International | Year: 2015

An endo-1,4-β-xylanase-encoding gene, xyn11B, was cloned from the thermophilic fungus Humicola insolens Y1. The gene encodes a multimodular xylanase that consists of a typical hydrophobic signal sequence, a catalytic domain of glycoside hydrolase (GH) family 11, a glycine-rich linker, and a family 1 carbohydrate binding module (CBM1). Deduced Xyn11B shares the highest identity of 74% with a putative xylanase from Podospora anserina S mat+. Recombinant Xyn11B was successfully expressed in Pichia pastoris and purified to electrophoretic homogeneity. Xyn11B had a high specific activity of 382.0 U mg-1 towards beechwood xylan and showed optimal activity at pH 6.0 and 50°C. Distinct from most reported acidic fungal xylanases, Xyn11B was alkaline-tolerant, retaining 30.7% of the maximal activity at pH 9.0. The K m and V max values for beechwood xylan were 2.2 mg mL-1 and 462.8 μmol min-1 mg-1, respectively. The enzyme exhibited a wider substrate specificity and produced a mixture of xylooligosaccharides. All these favorable enzymatic properties make Xyn11B attractive for potential applications in various industries. © 2015 Pengjun Shi et al. Source


Xiao X.-F.,Tongji University | Xiao X.-F.,Bei Fang University of Nationalities | Xiao X.-F.,Shanghai Institute of Technology | Xu J.-Y.,Shanghai Institute of Technology | Xiang W.-D.,Tongji University
Wuji Cailiao Xuebao/Journal of Inorganic Materials | Year: 2013

The dual-readout calorimeter is a new designed equipment to detect high-energy particles. It can collect the Cherenkov light and scintillation light at the same time, thus scientists can understand the particles comprehensively in the field of high-energy physics. Three types of dual-readout calorimeters have been designed so far: (1) Quartz fibers as Cherenkov emitter and plastic fibers as scintillator; (2) Undoped and doped scintillation crystals act as Cherenkov and scintillator respectively; (3) Scintillation crystal act as Cherenkov as well as scintillator. The last attracted much attention because it can avoid sampling fluctuations and enhance energy resolution of calorimeters. The progress on the scintillation crystals, including lead tungstate (PbWO4), bismuth germinate (Bi4Ge3O12), bismuth silicate (Bi4Si3O12) and lutetium aluminum garnet (Lu3Al5O12), were presented for the application of dual-readout calorimeter. Pr:PWO crystal and Bi4Si3O12 crystal have potential application in dual-readout calorimeter. It's easy to separate the Cherenkov and scintillation light for Bi4Si3O12 crystal because the short-wavelength cut-off of Bi4Si3O12 crystal is small in comparison with Bi4Ge3O12 crystal. Bi4Si3O12 crystal shows more advantages and its properties can be further optimized by doping rare earth ions. Source


Du Y.,Chinese Academy of Agricultural Sciences | Du Y.,Bei Fang University of Nationalities | Shi P.,Chinese Academy of Agricultural Sciences | Huang H.,Chinese Academy of Agricultural Sciences | And 4 more authors.
Bioresource Technology | Year: 2013

Three xylanase genes (xynA,. xynB,. xynC) of glycosyl hydrolase family 10 were identified in. Humicola insolens Y1. The deduced protein sequences showed the highest identity of ≤83% to known fungal xylanases and of ≤38% with each other. Recombinant XynA-C produced in. Pichia pastoris showed optimal activities at pH 6.0-7.0 and at high temperature (70-80. °C), and exhibited good stability over a broad pH range and temperatures at 60. °C. The gene. xynC produced by. H. insolens Y1 (named XynW) was similar in enzyme properties with XynC expressed by. Pichia. XynA exhibited better alkaline adaptation and thermostability, and had higher catalytic efficiency and wider substrate specificity. Under simulated mashing conditions, addition of XynA-C showed better performance on filtration acceleration (37.4%) and viscosity reduction (13.5%) than Ultraflo from Novozyme. Thus the three xylanases represent good candidates for application in the brewing industry. © 2012 Elsevier Ltd. Source

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