Chittagong, Bangladesh
Chittagong, Bangladesh

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Fakruddin M.,Bangladesh Institute of Technology | Mannan K.S.B.,Center For Food and Waterborne Diseases | Mazumdar R.M.,BCSIR Laboratories Chittagong | Afroz H.,Primeasia University
BMC Complementary and Alternative Medicine | Year: 2012

Background: There is wide spread interest in drugs derived from plants as green medicine is believed to be safe and dependable, compared with costly synthetic drugs that have adverse effects.Methods: We have attempted to evaluate the antioxidant, In vitro thrombolytic, antibacterial, antifungal and cytotoxic effects of Clausena heptaphylla (Rutaceae) stem bark extract ethanol extract.Results: Ethanolic stem bark extract of Clausena heptaphylla (CHET) contains flavonoids, alkaloids, saponins and steroids but it lacks tannins, anthraquinones and resins. Phenol content of the extract was 13.42 mg/g and flavonoid content was 68.9 mg/g. CHET exhibited significant DPPH free radical scavenging activity with IC50 value of 3.11 μg/ml. Reducing power of CHET was also moderately stronger. In the cytotoxicity assay, LC50 and Chi-square value of the ethanolic extract against brine shrimp nauplii were 144.1461 μg/ml and 0.8533 demonstrating potent cytotoxic effect of the extract. In vitro thrombolytic activity of CHET is significant with 45.38% clot lysis capability compared to that of Streptokinase (65.78%). In antibacterial screening, moderate zone of inhibition (6.5-9.0 mm in diameter) was observed against gram-positive Bacillus subtilis ATCC 11774, Bacillus cereus ATCC 10876, Staphylococcus aureus ATCC 25923, Bacillus polymyxa ATCC 842 and Bacillus megaterium ATCC 13578 and less promising zone of inhibition (3.0-4.5 mm in diameter) against gram-negative Salmonella typhi ATCC 65154, Shigella flexneri ATCC 12022, Proteus vulgaris ATCC 13315 and Escherichia coli ATCC 25922. Shigella sonnei ATCC 8992 did not show any sensitivity. The MIC values against these bacteria were ranged from 2,000 to 3,500 μg/ml. The extract showed significant zone of inhibition against Rhizopus oryzae DSM 2200, Aspergillus niger DSM 737 and Aspergillus ochraceus DSM 824 in antifungal assay.Conclusions: Further advanced research is necessary to isolate and characterize the chemical components responsible for the therapeutic properties of the plant. © 2012 Fakruddin et al.; licensee BioMed Central Ltd.

Bhuiyan M.N.I.,BCSIR Laboratories Chittagong | Begum J.,BCSIR Laboratories Chittagong | Nandi N.C.,BCSIR Laboratories Chittagong
Journal of Medicinal Plants Research | Year: 2010

Hyptis brevipes Poit. leaf and inflorescence essential oils obtained by hydrodistillation, were analyzed by gas chromatography mass spectroscopy (GC-MS). Fifty seven components were identified in the leaf oil. The major components were germacrene D (13.54%), caryophyllene (12.31%), phthalamide doxime (9.47%) and caryophyllene oxide (8.57%). Thirty seven components were identified in inflorescence oil with the main components being in caryophyllene oxide (45.09%), 1,5,5,8-tetramethyl-12-Oxabicyclo [9.1.0] dodeca-3,7-diene (4.95%), caryophyllene (4.79%) and α-bourbonene (4.20%). The compositions of both oils varied qualitatively and quantitatively. © 2010 Academic Journals.

Fakruddin M.,Bangladesh Institute of Technology | Mannan K.S.B.,Center for Food and Waterborne Diseases | Chowdhury A.,Bangladesh Institute of Technology | Mazumdar R.M.,BCSIR Laboratories Chittagong | And 3 more authors.
Journal of Pharmacy and Bioallied Sciences | Year: 2013

Nucleic acid amplification is a valuable molecular tool not only in basic research but also in application oriented fields, such as clinical medicine development, infectious diseases diagnosis, gene cloning and industrial quality control. A comperehensive review of the literature on the principles, applications, challenges and prospects of different alternative methods of polymerase chain reaction (PCR) was performed. PCR was the first nucleic acid amplification method. With the advancement of research, a no of alternative nucleic acid amplification methods has been developed such as loop mediated isothermal amplification, nucleic acid sequence based amplification, strand displacement amplification, multiple displacement amplification. Most of the alternative methods are isothermal obviating the need for thermal cyclers. Though principles of most of the alternate methods are relatively complex than that of PCR, they offer better applicability and sensitivity in cases where PCR has limitations. Most of the alternate methods still have to prove themselves through extensive validation studies and are not available in commercial form; they pose the potentiality to be used as replacements of PCR. Continuous research is going on in different parts of the world to make these methods viable technically and economically.

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