Sen M.,BCKV |
Legume Research | Year: 2017
The present study was undertaken to provide information on the nature and magnitude of genetic diversity among 30 mung bean genotypes for yield traits by using Mahalanobis’s D2 statistics. Thirty genotypes could be grouped in 6 clusters, cluster VI showed maximum intra-cluster distance while the highest inter-cluster distance was observed between cluster III and VI. Cluster II recorded highest means for seeds per pod, 100 seed weight, seed yield per plant and shelling %. The percent contribution towards genetic diversity was highest for shelling percentage (17.70) followed by seed yield per plant (16.55) and number of clusters per plant (14.71). From the divergence analysis, it may be concluded that the genotypes belonging to different clusters separated by high estimated statistical distance may be used in the hybridization programme for developing high yielding mung bean varieties. Five genotypes viz., PDM-11, TARM-2, TM-98-50, PDM 54 and Basanti could be identified as most useful in the future breeding programme. © 2017, Agricultural Research Communication Centre. All rights reserved.
Majumder C.,BCKV |
Haldar P.,BCKV |
Indian Journal of Ecology | Year: 2016
Due to delayed monsoon, farmers often fail to transplant rice seedlings at optimum age and cannot achieve the targeted yield. Afield experiment was carried out during kharif season of 2013 and 2014 with two types of nurseries viz. improved and recommended practice. The sprouted healthy seeds @ 10 g rn-2.e. 10 kg ha-1 and 50 g m-2 i.e. 50 kg ha-1 were spread over beds of improved and recommended nursery, respectively. Vigorous seedlings produced in the improved nurseries were more efficient to suppress the weed population. Lowest weed population was found with improved seedlings (IS) of 60 days. All the yield attributing characters and yield (5.281 ha-1) were found highest with IS of 30 days, but no significant difference were found between recommended seedlings (RS) of 30 days and IS of 60 days. No significant yield difference was noticed between 100% RDF and 125% RDF.
Banerjee A.,BCKV |
Banerjee A.,ICAR Research Complex for NEH Region |
Roy S.,BCKV |
Virus Genes | Year: 2012
Rice tungro bacilliform virus (RTBV) is a plant pararetrovirus. The large intergenic region (LIGR) of RTBV having a single transcriptional promoter produces more than genome length pregenomic RNA (pgRNA) which directs synthesis of circular double-stranded viral DNA and serves as a polycistronic mRNA. By computer-aided analysis of LIGR, the 11 RTBV isolates sequenced so far were compared with respect to structural organization of promoter and pgRNA 5′-leader. The results revealed only 74.90% identity at LIGR between 'Southeast Asian' (SEA) and 'South Asian' (SA) isolates of RTBV indicating considerable variation between two groups which was also reflected during analysis of promoter and leader sequence. The predicted promoter region of SA isolates exhibited major variations in terms of transcription start site and consensus sequences of cis motifs expecting further exploitation of promoter region of SA isolates. The reduced length of leader sequence along with less numbers and different arrangements of small open reading frames (sORFs) in case of SA isolates might have some alterations in the control of expression of ORF II and III between the two groups. In spite of these variations, the leader sequence of both SEA and SA type isolates showed formation of stable secondary or stem-loop structure having identical features for efficient translation. The conservation of sORF1 at seven nucleotides upstream of stable stem-loop, CU-rich sequence following the sORF1 stop codon and AU-rich shunt landing sequence immediately downstream of the secondary structure suggested conservation of ribosomal shunt mechanism in all RTBV isolates irrespective of their geographical distribution. © Springer Science+Business Media, LLC 2011.
Roy Barman A.,Regional Research Station Coastal Saline Zone |
Kamei A.,BCKV |
Archives of Phytopathology and Plant Protection | Year: 2016
Black rot of cabbage caused by Xanthomonas campestris pv. campestris is one of the most important diseases of crucifers worldwide. Expression of defence-related enzymes in cabbage in response to X. campestris pv. campestris was investigated in the current experiment. Among the defence-related enzymes (phynylalanine ammonia lyase, peroxidase, polyphenol oxidase, superoxide dismutase [SOD] and chitinase) and quantity of phenolic compounds studied in the present investigation, phenylalanine ammonia lyase (PAL), the key enzyme in the phenylpropanoid pathway was the first enzyme suppressed at three days after inoculation in X. campestris pv. campestris-cabbage system. Correlation analysis indicated that PAL and phenolic compounds are the two most important compounds determining the susceptibility of cabbage to X. campestris pv. campestris. Induction of peroxidase isoform-1 (Rf value: 0.059) and SOD isoform-1 (Rf value: 0.179) three days after pathogen inoculation implicated the role of these isozymes in susceptible cabbage – X. campestris pv. campestris interaction. This study demonstrates the susceptibility of cabbage to X. campestris pv. campestris is a result of declination of PAL and phenolic contents at biochemical level as a manifestation of increase in bacterial population at the cellular level within the host tissues. © 2016 Taylor & Francis
Das S.K.,Vivekananda Institute of Hill Agriculture |
Bulletin of Environmental Contamination and Toxicology | Year: 2012
Laboratory study was conducted with four different pesticides, viz. a fungicide (captan), two insecticides (malathion and cypermethrin) and a herbicide (alachlor) applied to a sandy loam soil at recommended dose to estimate their effect on microbial biomass carbon (MBC) and carbon mineralization (C min) in soil. The MBC content of the soil increased with time up to 30 days in cypermethrin as well as malathion treated soil. Then, it decreased and reached close to the initial level by 90th day. In alachlor treated soil, MBC increased through first 15 days, and then decreased to the initial level. In captan treated soil, the MBC showed a decreasing trend up to 45 days and then increased up to 90 days. Application of alachlor to the sandy loam soil showed an increasing trend while captan, malathion and cypermethrin treated soil decreased C min for the first 30 days and then increased afterwards. © 2012 Springer Science+Business Media, LLC.
African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines | Year: 2014
A field survey was conducted in the year, 2011 - 12 in Imphal valley of Manipur, on the use of herbs as ingredient sources for the preparation of traditional natural herbal shampoo referred to as 'Chinghi', by Meitei community. Methodological field survey and personal interview of local people aged between 30-70 years of both sexes using standard questionnaires were carried out to collect information on the plants use in the herbal shampoo preparation. The survey revealed the therapeutic application of 35 plant species representing 28 genera and 18 families available in the Imphal valley. Tree species contributed immensely, yielding 38%, while herbs 32%, shrubs 27%, and climbing shrubs 3%, respectively being the record of the total number of plants used as ingredient in herbal shampoo preparation. These natural shampoos are used for a wide range of common hair care like anti-ageing of the hair, blackness, shininess and smoothness of the hair. It is prepared from young leaves and tender stalk of shoot of trees or shrubs, or whole plant of the herbs and fresh fruits boiled with local sticky rice water locally called 'Chinghi'. Fermented lime peel is also used as a herbal shampoo. The study shows details of their scientific, common, and local names, including their family, parts used, habit of the plants, and the benefit to the hair health as a whole.
Das S.K.,Indian Agricultural Research Institute |
Mukherjee I.,Indian Agricultural Research Institute |
Bulletin of Environmental Contamination and Toxicology | Year: 2012
A field experiment was undertaken at Indian Agricultural Research Institute, New Delhi during kharif (rainy season) in the year 2010 to evaluate the residue persistence of flubendiamide in/on okra fruits following foliar application of Belt 39.35% SC formulation at 24 (standard dose) and 48 (double dose) g a.i. ha -1. After HPLC analysis study revealed that residues of flubendiamide in/on okra persisted till 5th and 7th day after the last spray at standard and double dose, respectively. The residues of flubendiamide were reported as parent compound, and des-iodo flubendiamide, a metabolite (photo product) of flubendiamide, was not detected in/on okra at any time during the study period. The initial deposits of 0.28 and 0.53 μg g -1 in/on okra fruits reached below determination level of 0.01 μg g -1 on the 7th and 10th day at standard and double dose, respectively. The half life of flubendiamide in/on okra fruits ranged from 4.7 to 5.1 days at standard and double dose, respectively. Soil sample collected from the treated field on the 15th day after the last spray revealed residues of flubendiamide or its metabolite below determination level (0.01 μg g -1) at single and double dose. © 2011 Springer Science+Business Media, LLC.
Journal of the Science of Food and Agriculture | Year: 2015
BACKGROUND: Rice is a potentially important route of human exposure to arsenic, especially in populations with rice-based diets. However, arsenic toxicity varies greatly with species. The initial purpose of the present study was to evaluate arsenic speciation in rice. RESULTS: It appeared very clear from the present study that inorganic arsenic shared maximum arsenic load in rice straw while in grains it is considerably low. As species recovered from rice grain and straw are principally As(III) and As(V) with a small amount of dimethylarsenic acid (DMA) and almost non-detectable monomethylarsonic acid (MMA) and arsenobetain (AsB). Discussion of the health risk of As in rice has largely been based on its inorganic arsenic content because these species have generally been considered to be more toxic than MMA and DMA and can be directly compared to As in drinking water, assuming equal bioavailability of inorganic As in the rice matrix and in water. The maximum dietary risk of exposure to inorganic arsenic through transplanted boro paddy in the present experiment was calculated to be almost 1706% of the provisional tolerable weekly intake for an adult of 60kg body weight. CONCLUSION: As species recovered from boro rice grain and straw are principally As(III) and As(V) with a small amount of DMA and almost non-detectable MMA and AsB. Reductions in total As load through organic amendments in boro rice grain and straw samples were manifested predominately through reduced accumulations of inorganic As species [As(III) and As(V)], between which As(V) accounted for the larger share. © 2014 Society of Chemical Industry.
Banerjee A.,BCKV |
Banerjee A.,ICAR Research Complex for NEH Region |
Roy S.,ICAR Research Complex for NEH Region |
Virus Genes | Year: 2011
A new isolate of Rice tungro bacilliform virus (RTBV) was collected from Chinsura, West Bengal, India. The full genome was sequenced and deposited to GenBank designating the new one as Chinsura isolate. The four open reading frames (ORFs) of the newisolate were comparedwith those of previously reported 'South-east Asian' (SEA) and 'South Asian' (SA) isolates emphasizing the ORF3, which is the largest and functionally most important gene of RTBV. In the ORFs, Chinsura isolate shared 90.0-100.0% identity at amino acid level with SA isolates, but only 58.76-88.63% identity with SEA isolates for the same. Similarly, the amino acid identity of ORFs between SEA and SA isolates ranged from 58.77 to 88.64, whereas within each group the corresponding valuewas[96.0%. The phylogenetic analysis based on nucleotide and amino acid sequences of each ORF made two broad clusters of SEA- and SA-types including Chinsura isolatewithin SAcluster.Moreover, the relative positions and length of functional domains corresponding to movement protein (MP), coat protein (CP), aspartate protease (PR) and reverse transcriptase/ribonuclease H (RT/RNase H) of ORF3 of Chinsura isolate were completely identical with SA isolates. The clustering pattern indicated strong influence of geographical habitat on genomic evolution. Comparison of ORF3 among all the isolates revealedmajor variations at nonfunctional regions in between the functional domains and at the hypervariable 30-terminal end of ORF3, while PR appeared to have evolved differentially in SA isolates expecting further characterization. © Springer Science+Business Media, LLC 2011.
Ghosh D.,Barrackpore Rastraguru Surendranath College |
Indian Chemical Engineer | Year: 2016
Carotenoids are the most common pigments in nature possessing high nutraceutical value. The objective of the present work was to obtain a good yield of carotenoids from pumpkin tissues, using cellulase and pectinase enzymes. Various parameters such as concentration of enzymes and time of incubation were optimised, to improve the yield of carotenoid from pumpkins. Enzyme-aided extraction of carotenoid from whole pumpkins under optimised conditions resulted in an increase in the carotenoid yield by 33.3% (w/w) in cellulase-treated sample and 28.6% (w/w) in case of pectinase-treated sample. Extraction from pumpkin peel under optimised conditions showed a remarkable increase in the yield of carotenoid by 77.7% (w/w) and 71.4% (w/w), for cellulase- and pectinase-treated samples, respectively. © 2015 Indian Institute of Chemical Engineers.