Chouinard N.T.,BC Institute of Technology |
Fix C.,St. Pauls Hospital |
Swan H.,Charles Sturt University
Journal of Diagnostic Medical Sonography | Year: 2010
A study was undertaken to evaluate the use of 3D multiplanar reconstructed (MPR) images to reduce the scan time for endovaginal studies, thereby potentially reducing sonographer strain and possibly improving patient workflow. The scope of the study was limited to evaluation of the endometrium. A group of 42 patients with dysfunctional uterine bleeding was selected for the study. After initial transabdominal examination on each patient, an endovaginal study of the endometrium was performed using 3D data collection; this was followed by a complete conventional 2D endovaginal scan. The scan times and endometrial measurements for 3D and 2D methods were compared. Staff members were interviewed for feedback on the experience and for future protocol recommendations.
Brown P.N.,BC Institute of Technology |
Yu R.,BC Institute of Technology |
Kuan C.H.,BC Institute of Technology |
Finley J.,BC Institute of Technology |
And 2 more authors.
Journal of AOAC International | Year: 2014
A single-laboratory validation (SLV) was conducted on an HPLC method for the detection and quantification of aloin A and aloin B in Aloe vera raw materials and finished products. An extraction procedure using sonication with an acidified solvent was used for solid test materials while liquid test materials only required dilution, if necessary, prior to filtration and analysis. Separation was achieved using a fused core C18 column in 18 min under isocratic elution conditions allowing for a single analyte (aloin A) calibration curve to quantify both aloins. Adequate chromatographic resolution (Rs >1) was achieved for aloin A and aloin B. The calibration curves for aloin A exhibited coefficients of determination (r2) of >99.9% over the linear range of 0.3-50 μg/mL. The LOD values were 0.092 and 0.087 μg/mL, and LOQ 0.23 and 0.21 μg/mL for aloin A and aloin B, respectively. Repeatability studies were performed on nine test materials on each of 3 separate days, with five of the test materials determined to be above the LOQ having repeatability RSD (RSDr) values ranging from 0.61 to 6.30%. Method accuracy was determined through a spike recovery study on both liquid and solid matrixes at three different levels: low, medium, and high. For both aloins, the recovery in the liquid matrix ranged from 92.7 to 106.3% with an RSDr of 0.15 to 4.30%, while for the solid matrix, the recovery ranged from 84.4 to 108.9% with an RSDr of 0.23 to 3.84%. Based on the results of the SLV study, it is recommended that this method be evaluated for reproducibility through a collaborative study.
Mudge E.M.,BC Institute of Technology |
Jones A.M.P.,University of Guelph |
Brown P.N.,BC Institute of Technology |
Brown P.N.,University of Guelph
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2015
Pyrrolizidine alkaloids (PAs) are a class of naturally occurring compounds produced by many flowering plants around the World. Their presence as contaminants in food systems has become a significant concern in recent years. For example, PAs are often found as contaminants in honey through pollen transfer. A validated method was developed for the quantification of four pyrrolizidine alkaloids and one pyrrolizidine alkaloid N-oxide in plants and honey grown and produced in British Columbia. The method was optimised for extraction efficiency from the plant materials and then subjected to a single-laboratory validation to assess repeatability, accuracy, selectivity, LOD, LOQ and method linearity. The PA content in plants ranged from1.0 to 307.8 µg/g with repeatability precision between 3.8 and 20.8% RSD. HorRat values were within acceptable limits and ranged from 0.62 to 1.63 for plant material and 0.56–1.82 for honey samples. Method accuracy was determined through spike studies with recoveries ranging from 84.6 to 108.2% from the raw material negative control and from 82.1–106.0 % for the pyrrolizidine alkaloids in corn syrup. Based on the findings in this single-laboratory validation, this method is suitable for the quantitation of lycopsamine, senecionine, senecionine N-oxide, heliosupine and echimidine in common comfrey (Symphytum officinale), tansy ragwort (Senecio jacobaea), blueweed (Echium vulgare) and hound’s tongue (Cynoglossum officinale) and for PA quantitation in honey and found that PA contaminants were present at low levels in BC honey. © 2015 Taylor & Francis.