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Saskatoon, Canada

Kavanagh V.B.,University of Alberta | Hills M.J.,Grant MacEwan University | Goyal A.,Agriculture and Agri Food Canada | Goyal A.,Bayer Crop Science Inc. | And 4 more authors.
Transgenic Research | Year: 2013

Triticale is being considered as a bioindustrial crop in Canada using genetic modification. Because related spring wheat (Triticum aestivum) and durum wheat (T. durum) may exhibit synchronous flowering and grow in proximity, determination of interspecific gene flow when triticale is the pollen donor is necessary to evaluate potential risk. Pollen-mediated gene flow risk assessments generally rely on phenotypic markers to detect hybridization but DNA markers could be powerful and less ambiguous in quantifying rare interspecific gene flow. Six cultivars representing four species [spring wheat, durum wheat, triticale and rye (Secale cereale)] were screened with 235 spring wheat and 27 rye SSR markers to evaluate transferability and polymorphism. Fifty-five polymorphic markers were used in conjunction with morphological characterization to quantify interspecific gene flow from a blue aleurone (BA) triticale line to two spring wheat cultivars (AC Barrie and AC Crystal) and one durum wheat cultivar (AC Avonlea). Approximately 1.9 Million seeds from small plot experiments were visually screened in comparison with known hybrid seed. In total 2031 putative hybrids were identified and 448 germinated. Morphological analysis of putative hybrid plants identified five hybrids while molecular analysis identified 11 hybrids and two were common to both. Combined, 14 hybrids were confirmed: 10 spring wheat × triticale (0.0008 % of harvested seed): seven AC Barrie × BA triticale (0.001 %) and three AC Crystal × BA triticale (0.0005 %); and four durum wheat × triticale (0.0006 %). The occurrence of rare hybrids does not present a substantial risk to the development of GM triticale. © 2013 Springer Science+Business Media Dordrecht.

Samanta A.,Bidhan Chandra Krishi Viswavidyalaya | Dhote V.W.,Bidhan Chandra Krishi Viswavidyalaya | Patra S.,ICAR Research Complex for NEH Region | Biswas M.,Bayer Crop Science Inc.
Research on Crops | Year: 2013

Field experiment was conducted at Horticultural Research Farm, Mondouri, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal during 2007-08 and 2008-09 to evaluate the effectiveness of different spray treatments viz., T 1 : Spraying of imidacloprid (0.005%) at panicle emergence, T 2 : T1+Spraying of endosulfan (0.07%) at 21 days after spraying of imidacloprid (0.005%) spray and T3 : T 2+Spraying of endosulfan (0.07%) at 15 days after first spraying of endosulfan (0.07%) along with untreated check against mango hopper. All insecticidal treatments were found to be significantly superior in reducing the hopper population as well as increase in fruit yield over untreated check. Among these treatments, the triple spray (T3) was found to be most effective, which recorded lowest hopper population (5.48 hoppers/panicle) as well as highest fruit yield both at marble stage (197.62 fruits/100 panicles) and mature stage (96.4 kg/plant) and avoidable fruit loss (82.9%) followed by T. (double spray) and T (single spray), respectively.

Tessenderlo Kerley Inc., Bayercropscience Inc., Rhone Poulenc Ag Company and Union Carbide Corporation | Date: 1958-12-30


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