Bati Akdeniz Agricultural Research Institute

Antalya, Turkey

Bati Akdeniz Agricultural Research Institute

Antalya, Turkey
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Sogut M.A.,Suleyman Demirel University of Turkey | Devran Z.,Bati Akdeniz Agricultural Research Institute
Nematropica | Year: 2011

Root lesion nematodes are important migratory endoparasitic nematodes attacking temperate fruits in the West Mediterranean region of Turkey. A rapid and accurate method to identify Pratylenchus to the species level is necessary to develop management strategies. Seventy-eight populations of the root lesion nematode were collected from the temperate fruit production region in Turkey, including fruit orchards in Isparta and Antalya provinces. Species-specific primers and rDNA primers were used to identify Pratylenchus spp. Distribution ratios of the sampled root lesion nematode populations were 50%, 45%, 2.5% and 2.5% for P. thornei, P. neglectus, P. penetrans, and P. crenatus, respectively. The present study indicated that P. thornei and P. neglectus were widespread on temperate fruits in the West Mediterranean region of Turkey.

Devran Z.,Bati Akdeniz Agricultural Research Institute | Sogut M.A.,Suleyman Demirel University of Turkey | Mutlu N.,Akdeniz University
Phytopathologia Mediterranea | Year: 2010

Rootstocks have been effective against many soil-borne pathogens in protected tomato production. Rootstocks with heat-stable root-knot nematode resistance may prolong the production season since the root-knot nematode resistance gene Mi-1.2 irreversibly breaks down at soil temperatures above 28°C. The objective of this study was to investigate the effect of soil temperature on root-knot nematode resistance conferred by two genes of tomato, using some commercial tomato cultivars, rootstocks, and PI lines. The response of these genes against Meloidogyne incognita race 2 was studied in two commonly used rootstock cv. Beaufort and Vigomax, in tomato cultivars Astona RN F1 and Simita F1, and in Solanum lycopersicum L. accessions PI126443 and PI270435, known to possess heat-stable nematode resistance, at 24°C and 32°C under controlled conditions. Each plant was inoculated with 1000 M. incognita race 2 second-stage juveniles (J2s) and its response was evaluated 8 weeks post inoculation. The presence of the Mi-1.2 gene was determined with molecular markers. Astona RN F1, Vigomax, Beaufort, PI126443 and PI 270435 which carried the Mi-1.2 gene were resistant to Meloidogyne incognita race 2 at 24°C. The egg masses and J2s were significantly fewer in these lines than in the susceptible Simita F1 at 24°C, and there were no significant differences among resistant plants. In contrast, there were significant differences in the galling index among heat-stable sources and plants containing the Mi-1.2 gene. Simita F1, Astona RN F1 and the rootstocks had a susceptible reaction to M. incognita race 2 at 32°C, but PI 126443 and PI 270435 were resistant. However, at this temperature there were significant differences in the number of juveniles in the soil, the egg mass and the galling index between the heat-stable and the heat-unstable plants.

Devran Z.,Bati Akdeniz Agricultural Research Institute | Sogut M.A.,Suleyman Demirel University of Turkey
Phytoparasitica | Year: 2010

Root-knot nematodes (Meloidogyne spp.; RKN) are one of the most important pathogens of vegetables in Turkey. Assessing the existing virulent RKN populations is of importance for pathogen mapping in the west Mediterranean region of Turkey. Therefore, 95 populations of RKN were collected from different protected vegetable-growing locations in the region. Pure cultures were obtained and identified by means of species-specific primers. Virulence of the populations against the Mi-1 gene conferring resistance to Meloidogyne incognita, M. javanica and M. arenaria was determined according to their egg masses and gall rating on resistant and susceptible tomato varieties. Results showed that seven populations of M. incognita and six populations of M. javanica were able to overcome the resistance controlled by the Mi-1 gene. The frequency of virulent populations of M. incognita and M. javanica collected from different protected-grown vegetables was 11.7% and 21.4%, respectively. To our knowledge, this is the first report of populations of RKN virulent to the Mi-1 gene in Turkey. © 2010 Springer Science+Business Media B.V.

Devran Z.,Bati Akdeniz Agricultural Research Institute | Baysal O.,Muǧla University
Biologia | Year: 2012

Southern root knot nematode Meloidogyne incognita is the most widespread-species, causing serious yield losses in protected vegetables fields in the West Mediterranean region of Turkey. The knowledge of genetic variation within M. incognita is required for disease management and improvement of resistant varieties by breeding programs. In the present study, the isolates were classified into different groups based on sequence-related amplified polymorphism (SRAP) fingerprints. To our knowledge, this is the first study carried out on the characterization of M. incognita isolates using SRAP. The schematic diagram by tested primers to differentiate of M. incognita isolates was formed in discrimination of nematodes as an effective molecular tool since it is cost effective and easiness. Data presents a genetic variation on root-knot nematode species. These selected SRAP markers can be used to follow genetic structure and differentiation on M. incognita isolates in a certain region. © 2012 © Versita Warsaw and Springer-Verlag Wien.

Karaca M.,Akdeniz University | Ince A.G.,Akdeniz University | Aydin A.,Akdeniz University | Ay S.T.,Bati Akdeniz Agricultural Research Institute
Journal of the Science of Food and Agriculture | Year: 2013

Background: The Lamiaceae family contains many high-valued medicinal, aromatic and ornamental plant species. Several members of the genera in this family are under heavy pressure of collection for commercial use. DNA markers such as microsatellites could be used to identify commercially important genotypes and to select high-yielding ones for development of new varieties. Results: A total of 12 432 expressed sequence tags (ESTs) from Salvia fruticosa, S. miltiorrhiza, S. sclarea and Stenogyne rugosa were analyzed. A total of 6216 ESTs were found to be unique according the redundancy test used. Results of this study indicated that the use of redundant ESTs in comparison to non-redundant ESTs was advantageous in terms of higher cross-genera transferability of the markers. A total of 75 EST-microsatellite primer pairs were tested using two different polymerase chain reaction amplification profiles and 52 were found to be cross-genera transferable. Cross-genera transferability of the e-microsatellite primer pairs varied from one species to 12 species tested. It was noted that cross-genera transferability of e-microsatellite primer pairs decreased as the evolutionary distance between the sources and target species increased. Conclusion: This study indicated that EST resources from Salvia spp. and Stenogyne rugosa could be successfully used to identify cross-genera transferable e-microsatellite markers for uncharacterized genomes of the genera in the Lamiaceae family. These e-microsatellite markers could allow one to perform comparative analyses of population structure and genomic studies, and facilitate comparative linkage mapping in the genera studied. E-microsatellite primer pairs reported in this manuscript are equivalent to a total of 135 e-microsatellite primer pairs since many e-microsatellite primer pairs show cross-genera transferability. © 2012 Society of Chemical Industry.

Yilmaz S.,Bati Akdeniz Agricultural Research Institute | Celik I.,Bati Akdeniz Agricultural Research Institute | Zengin S.,Bati Akdeniz Agricultural Research Institute
International Journal of Plant Production | Year: 2011

Combining effects of soil solarization and grafting on soilborne pathogens, plant height and yield in cucumber were compared in a greenhouse of Bati Akdeniz Agricultural Research Institute located in Antalya in 2008 fall season. The experiment was set in split plot design containing; 1, 2, 4 and 5 months soil solarization (MSS), and non-solarized control plots (NSC) on which grafted Maximus F1+ Bergama F1 and non-grafted Bergama F1 were grown. Almost all of plants grown in nonsolarized plots were infected with root-knot nematodes with severe root damages; however, only a few plants were affected by this nematode in 4 and 5 months solarized plots with very slight root galls. No resistance to root-knot nematodes was observed in both grafted and non-grafted plants. Almost half of the plants grown on non-solarized control plots were infected with Fusarium oxysporum f. sp. cucumerinum with relatively slight disease symptoms. In conclusion, combining solarization with grafting significantly promoted early flowering time, plant vigor, early and total yields and reduced nematode and fusarium wilt damages in this study.

Asri F.O.,Bati Akdeniz Agricultural Research Institute | Sonmez S.,Akdeniz University
Journal of Food, Agriculture and Environment | Year: 2010

Potassium and iron are the main elements that are of great importance in soilless medium in terms of tomato yield and fruit quality. For this reason, the effects of these elements on yield and fruit quality parameters were investigated. Three different combinations of potassium ( 150,300 and 450 mg l-1) and iron (1,2 and 3 mg l-1') ratios were evaluated. The experiment was conducted according to completely randomized factorial design with 4 replicates. The interaction between potassium and iron applications was significant in yield, total soluble solids, fruit firmness and a*-value. Moreover, titratable acidity, total soluble solids content and pH of fruit juice showed increase by ascending K applications. There were no effects of K and Fe applications on ascorbic acid, fruit diameter, L* and b* values. These results indicated interactive effects between potassium and iron applications on fruit quality and yield are more complex structure. Therefore, detailed investigations are required in different growing medium, ecological condition and on tomato variety.

Kabas O.,Bati Akdeniz Agricultural Research Institute
International Journal of Food Properties | Year: 2010

The grade of injures can be determined or measured by bruise volume using a sectioning and imaging analysis technique and different methods for calculating the size of pear bruises. The calculation methods were not consistent, with large calculation errors at small bruise sizes. The calculation of bruise volume was improved when the surface shape of the bruise was an ellipse instead of a circle. In this study, various methods are recommended for the calculation of the bruise volume depending on the range of the bruise sizes being investigated. Copyright © 2010 Taylor & Francis Group, LLC.

Aydinsakir K.,Bati Akdeniz Agricultural Research Institute | Erdal S.,Bati Akdeniz Agricultural Research Institute | Buyuktas D.,Akdeniz University | Bastug R.,Akdeniz University | Toker R.,Bati Akdeniz Agricultural Research Institute
Agricultural Water Management | Year: 2013

The objective of the present study was to determine the mechanisms of tolerance of two corn (Zea mays L.) genotypes (Şafak and Ant-İ90) to water stress under five different irrigation treatments designated as full irrigation (I100) with no water stress and slight (I75), mild (I50), severe water stress (I25) and no irrigation (I0) treatments. The results showed that different irrigation levels applied has statistically significant effect on yield components such as anthesis-silking interval, plant height, ear diameter, ear length, kernel number and 1000 grain weight, except for ear number. In both of the genotypes, water deficit stress significantly (P≤0.01) increased glucose, fructose, and sucrose contents while it decreased protein content. The maximum grain yield was obtained from Şafak genotype under full irrigation, slight, mild, severe and full water deficit stress as much as 9.35tha-1, 8.34tha-1, 7.89tha-1, 5.56tha-1 and 3.63tha-1, respectively. The highest water use was observed in I100 treatment as 738.1mm for Şafak genotype, while the lowest was found in I0 treatment as 260.0mm for Ant-İ90 genotype. The highest water use efficiency was found in I50 as 15.7kgha-1mm-1 for Şafak genotype, and the lowest one was found in I0 as 5.5kgha-1mm-1 for Ant-İ90. It was concluded that Şafak was more tolerant genotype to water stress than that of Ant-İ90. © 2013 Elsevier B.V.

Kurbetli I.,Bati Akdeniz Agricultural Research Institute
Phytoparasitica | Year: 2014

Sweet cherry is a major commercial crop in Turkey, the most important producer of the fruit worldwide. Sweet cherry decline was observed in an orchard in Ankara province of Turkey. Affected young trees exhibited reduced tree vigor, yellowing and wilting of leaves, and dieback symptoms resulted in tree death. A Phytophthora sp. was consistently isolated from necroses that appeared on taproots and crowns. The pathogen was identified as Phytophthora cryptogea based on several morphological features and DNA sequence of the internal transcribed spacer (ITS) region. P. cryptogea was pathogenic on excised shoots and 1-year-old cherry rootstocks. This is the first report of P. cryptogea causing disease of sweet cherry in Turkey. © 2014 Springer Science+Business Media Dordrecht.

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