Basic Research Laboratory

Salvador, Brazil

Basic Research Laboratory

Salvador, Brazil
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Leite M.B.N.L.,Federal University of Bahia | Da Cruz A.L.,Federal University of Bahia | Rodrigues L.E.A.,Basic Research Laboratory | Yamashita S.R.,Basic Research Laboratory | Nascimento I.A.,Federal University of Bahia
Bulletin of Environmental Contamination and Toxicology | Year: 2015

This study estimated end compared the potential toxic effects of the water-soluble fractions (WSF) of biodiesel (B100), diesel and the commercial biodiesel (B5) on Oreochromis niloticus. After a 24 h-exposition to WSF-0 % (control) and WSF-serial concentrations of 4.6 %, 10 %, 22 %, 46 % and 100 %, samples of gill and liver of the exposed fishes were fixed in Bouin's solution, processed, stained using hematoxylin/eosin and analyzed by light-microscopy. WSF-hydrocarbons and methanol contents, analyzed by gas chromatography, were checked against the occurrence of abnormal histopathological alterations. These were not found in the control and WSF-4.6 % exposed fishes, while exposures to or above 10 %-WSF resulted in histopathological alterations whose severity increased in a dose-dependent manner, being higher in fishes exposed to WSF-diesel, or WSF-B5 when compared to biodiesel. These results, which were corroborated by the chemical analyses, highlighted the histological technique as an appropriate diagnostic tool that can be used for the preservation of water bodies' quality. © 2015 Springer Science+Business Media New York.


PubMed | Basic Research Laboratory and Federal University of Bahia
Type: Journal Article | Journal: Bulletin of environmental contamination and toxicology | Year: 2015

This study estimated end compared the potential toxic effects of the water-soluble fractions (WSF) of biodiesel (B100), diesel and the commercial biodiesel (B5) on Oreochromis niloticus. After a 24 h-exposition to WSF-0% (control) and WSF-serial concentrations of 4.6%, 10%, 22%, 46% and 100%, samples of gill and liver of the exposed fishes were fixed in Bouins solution, processed, stained using hematoxylin/eosin and analyzed by light-microscopy. WSF-hydrocarbons and methanol contents, analyzed by gas chromatography, were checked against the occurrence of abnormal histopathological alterations. These were not found in the control and WSF-4.6% exposed fishes, while exposures to or above 10%-WSF resulted in histopathological alterations whose severity increased in a dose-dependent manner, being higher in fishes exposed to WSF-diesel, or WSF-B5 when compared to biodiesel. These results, which were corroborated by the chemical analyses, highlighted the histological technique as an appropriate diagnostic tool that can be used for the preservation of water bodies quality.


Seo D.-W.,U.S. National Cancer Institute | Seo D.-W.,Kangwon National University | Saxinger W.C.,Basic Research Laboratory | Guedez L.,U.S. National Cancer Institute | And 3 more authors.
Peptides | Year: 2011

Tissue inhibitor of metalloproteinases-2 (TIMP-2) inhibits angiogenesis by several mechanisms involving either MMP inhibition or direct endothelial cell binding. The primary aim of this study was to identify the TIMP-2 region involved in binding to the previously identified receptor integrin α3β1, and to determine whether synthetic peptides derived from this region retained angio-inhibitory and tumor suppressor activity. We demonstrated that the N-terminal domain of TIMP-2 (N-TIMP-2) binds to α3β1 and inhibits vascular endothelial growth factor-stimulated endothelial cell growth in vitro, suggesting that both the α3β1-binding domain and the growth suppressor activity of TIMP-2 localize to the N-terminal domain. Using a peptide array approach we identify a 24 amino acid region of TIMP-2 primary sequence, consisting of residues Ile43-Ala66, which shows α3β1- binding activity. Subsequently we demonstrate that synthetic peptides from this region compete for TIMP-2 binding to α3β1 and suppress endothelial growth in vitro. We define a minimal peptide sequence (peptide 8-9) that possesses both angio-inhibitory and, using a murine xenograft model of Kaposi's sarcoma, anti-tumorigenic activity in vivo. Thus, both the α3β1- binding and the angio-inhibitory activities co-localize to a solvent exposed, flexible region in the TIMP-2 primary sequence that is unique in amino acid sequence compared with other members of the TIMP family. Furthermore, comparison of the TIMP-2 and TIMP-1 protein 3-D structures in this region also identified unique structural differences. Our findings demonstrate that the integrin binding, tumor growth suppressor and in vivo angio-inhibitory activities of TIMP-2 are intimately associated within a unique sequence/structural loop (B-C loop). © 2011 Elsevier Inc.


Mikovits J.A.,University of Nevada, Reno | Lombardi V.C.,University of Nevada, Reno | Ruscetti F.W.,Basic Research Laboratory
Therapy | Year: 2010

Despite the fact that xenotropic murine leukemia virus-related virus (XMRV) research is in its infancy, considerable attention has been focused on this recently discovered human retrovirus because of the surprising association of XMRV with two very different diseases: prostate cancer and myalgic encephalomyelitis/chronic fatigue syndrome. Like other retroviral infections, XMRV integrates into host-cell DNA and thus endures for a lifetime. Little is known about host-cell interactions, reservoirs of infection or the lifecycle of XMRV. Information on murine xenotropic viruses, as well as current research on cellular tropism and cis-acting glucocorticoid response elements, provides intriguing clues for viral persistence, mechanisms of pathogenesis and opportunities for XMRV as a diagnostic biomarker and therapeutic target in myalgic encephalomyelitis/chronic fatigue syndrome. © 2010 Future Medicine Ltd.


Limou S.,Basic Research Laboratory | Nelson G.W.,Core Informatics | Lecordier L.,Free University of Colombia | An P.,Basic Research Laboratory | And 8 more authors.
Kidney International | Year: 2015

A third of African Americans with sporadic focal segmental glomerulosclerosis (FSGS) or HIV-associated nephropathy (HIVAN) do not carry APOL1 renal risk genotypes. This raises the possibility that other APOL1 variants may contribute to kidney disease. To address this question, we sequenced all APOL1 exons in 1437 Americans of African and European descent, including 464 patients with biopsy-proven FSGS/HIVAN. Testing for association with 33 common and rare variants with FSGS/HIVAN revealed no association independent of strong recessive G1 and G2 effects. Seeking additional variants that might have been under selection by pathogens and could represent candidates for kidney disease risk, we also sequenced an additional 1112 individuals representing 53 global populations. Except for G1 and G2, none of the 7 common codon-altering variants showed evidence of selection or could restore lysis against trypanosomes causing human African trypanosomiasis. Thus, only APOL1 G1 and G2 confer renal risk, and other common and rare APOL1 missense variants, including the archaic G3 haplotype, do not contribute to sporadic FSGS and HIVAN in the US population. Hence, in most potential clinical or screening applications, our study suggests that sequencing APOL1 exons is unlikely to bring additional information compared to genotyping only APOL1 G1 and G2 risk alleles.


PubMed | Basic Research Laboratory, Core Informatics, Free University of Colombia, University of Puerto Rico at Mayaguez and 2 more.
Type: Journal Article | Journal: Kidney international | Year: 2015

A third of African Americans with sporadic focal segmental glomerulosclerosis (FSGS) or HIV-associated nephropathy (HIVAN) do not carry APOL1 renal risk genotypes. This raises the possibility that other APOL1 variants may contribute to kidney disease. To address this question, we sequenced all APOL1 exons in 1437 Americans of African and European descent, including 464 patients with biopsy-proven FSGS/HIVAN. Testing for association with 33 common and rare variants with FSGS/HIVAN revealed no association independent of strong recessive G1 and G2 effects. Seeking additional variants that might have been under selection by pathogens and could represent candidates for kidney disease risk, we also sequenced an additional 1112 individuals representing 53 global populations. Except for G1 and G2, none of the 7 common codon-altering variants showed evidence of selection or could restore lysis against trypanosomes causing human African trypanosomiasis. Thus, only APOL1 G1 and G2 confer renal risk, and other common and rare APOL1 missense variants, including the archaic G3 haplotype, do not contribute to sporadic FSGS and HIVAN in the US population. Hence, in most potential clinical or screening applications, our study suggests that sequencing APOL1 exons is unlikely to bring additional information compared to genotyping only APOL1 G1 and G2 risk alleles.


Chae B.G.,Basic Research Laboratory | Kim H.T.,Basic Research Laboratory
Physica B: Condensed Matter | Year: 2010

V1 - x Wx O2 thin films with various W concentrations were successfully deposited on sapphire by the sol-gel method to investigate the effects of W doping on the transition properties. All the films are grown to be (0 4 0)-preferred orientation and have well-formed grains. The transition temperature (Tc) is significantly reduced with increasing W concentration and the rate of Tc reduction reaches to 13.8 K per 1 at% W. The resistivity in the insulator state clearly decreases with doping amount due to the enhancement of the charge carriers. The doped-films have well-formed impurity level with an activation energy of 0.08 eV from the bottom of the conduction band. The excited charge carriers to the conduction band on the verge of the transition should play a role in the insulator-to-metal transition for the V1 - x Wx O2 film. The generated free carriers screen the Coulomb repulsion of the electrons and lead to the metallic state. © 2009 Elsevier B.V. All rights reserved.

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