Li X.-F.,Basic Medical College |
Hu S.-S.,Medical and Biological Research Center |
Gao J.-J.,Medical and Biological Research Center |
Zhu X.-T.,Basic Medical College
Modern Food Science and Technology | Year: 2013
The sulforhodamine B (SRB) assay was employed to evaluate the anti-tumor effect of petroleum ether extracts from Fomitopsis officinalis (Vill.:Fr.) Bond (PEFO) on SMCC-7721, SGC-7901, and Hep-2 cell lines in vitro. The results showed PEFO inhibited the growth of three kinds of cells in dose-dependent manner with the IC50 values on SMCC-7721, SGC-7901, and Hep-2 cells being of 58.74, 74.13 and 83.18 μg/mL, respectively. The SMCC-7721 cells exposed to PEFO obviously changed cell morphology and cellular ultrastructure. The SMCC-7721 cell presented wrinkled nuclei, poor cell adherent growth and cell lysis, while mitochondria swell and a large number of vacuoles in cytoplasm were observed by transmission electron microscope. The flow cytometry results revealed that SMCC-7721cell decreased in S-phase and increased in G0/G1-phase, which arrested in G0/G1 phase. The study demonstrated that PEFO could inhibit cell proliferation in vitro, and the anti-tumor activity of PEPO might be achieved by inhibition of tumor cellular aerobic metabolism and intervention cell cycle.
PubMed | Peking University and Basic Medical College
Type: Journal Article | Journal: The Journal of biological chemistry | Year: 2016
Hyperhomocysteinemia (HHcy) is a condition characterized by an abnormally high level of homocysteine, an inflammatory factor. This condition has been suggested to promote insulin resistance. To date, the underlying molecular mechanism remains largely unknown, and identifying novel therapeutic targets for HHcy-induced insulin resistance is of high priority. It is well known that intermedin (IMD), a calcitonin family peptide, exerts potent anti-inflammatory effects. In this study, the effects of IMD on HHcy-induced insulin resistance were investigated. Glucose tolerance and insulin tolerance tests were performed on mice treated with IMD by minipump implantation (318 ng/kg/h for 4 weeks) or adipocyte-specific IMD overexpression mice (Adipo-IMD transgenic mice). The expression of genes and proteins related to M1/M2 macrophages and endoplasmic reticulum stress (ERS) was evaluated in adipose tissues or cells. The expression of IMD was identified to be lower in the plasma and adipose tissues of HHcy mice. In both IMD treatment by minipump implantation and Adipo-IMD transgenic mice, IMD reversed HHcy-induced insulin resistance, as revealed by glucose tolerance and insulin tolerance tests. Further mechanistic study revealed that IMD reversed the Hcy-elevated ratio of M1/M2 macrophages by inhibiting AMP-activated protein kinase activity. Adipo-IMD transgenic mice displayed reduced ERS and lower inflammation in adipose tissues with HHcy. Soluble factors from Hcy-treated macrophages induced adipocyte ERS, which was reversed by IMD treatment. These findings revealed that IMD treatment restores the M1/M2 balance, inhibits chronic inflammation in adipose tissues, and improves systemic insulin sensitivity of HHcy mice.
Bing Z.,Zhengzhou University |
Linlin L.,Henan Province Peoples Hospital |
Jianguo Y.,Basic Medical College |
Shenshen R.,Zhengzhou University |
And 2 more authors.
Molecular Biology Reports | Year: 2012
The occurrence of PFD is closely related with elasticity, toughness, and functional changes of the connective tissue of the pelvic support tissue. This study aims to evaluate the effect of mechanical stretch on the differentiation of BMSCs with a co-culture with pelvic ligament fibroblasts. BMSCs was isolated and identified from 7 day SPF SD rats. Rat pelvic ligament fibroblasts were obtained from rat pelvic ligament. The fourth passage of fibroblasts was subjected to 10% deformation with 1 Hz, 12 h periodic one-way mechanical stretch stimulation, and the cells were then co-cultured with BMSCs. The longer co-culture and co-culture with mechanical stretch (i.e. 6 and 12 days) induced more expression of elastin, LOX, and Fibulin-5, compared to the groups without stimulation. Compared to co-culture group each, Co-culture with mechanical stretch stimulation group induced significant expression of elastin, LOX, and Fibulin-5, both in 3, 6 and 12 days co-culture groups (P<0.05). However, there were no significant differences among 3, 6, and 12 days control groups. These results suggest that in an indirect co-culture system, pelvic ligament fibroblasts with mechanical stretch stimulation can promote BMSCs differentiation, reflecting in the increased expression of elastin, LOX, and Fibulin-5. © Springer Science+Business Media B.V. 2011.
Hong F.-F.,Nanchang University |
Guo F.-X.,Nanchang University |
Zhou Y.,Basic Medical College |
Min Q.-H.,First Affiliated Hospital of Obstetrics and Gynecology |
And 7 more authors.
Journal of Ethnopharmacology | Year: 2015
Ethnopharmacological relevance The pathogenesis of thromboangiitis obliterans (TAO) has not been fully elucidated until now. Shenfu injection (SFI), a traditional Chinese formula has been widely used clinically for the treatment of cardiovascular diseases for more than two decade. Our previous results first suggested that SFI can cause a significant therapeutic effect on experimental TAO model rats. This experiment was designed to further investigate the protective effect of SFI on VEC damaged by hydrogen peroxide (H2O2) oxidative stress in vitro. Meterials and methods The cell viability was evaluated by the MTT assay, the activities of SOD and GSH-PX and the content of MDA in the supernatants of the cultured ECV304 cells were evaluated by a colorimetry method, cell apoptosis was detected by flow cytometry and an AO/EB double staining method. The protein expressions of Bcl2, Bax and caspase-3 were examined by Western blotting. Results When compared with control group, lower survival rate of ECV304 cells was observed in H2O2 group (p<0.01); 20 μl/ml, 30 μl/ml and 40 μl/ml SFI increased the survival rate of ECV304 cells under H2O2 oxidative stress (p<0.05 and p<0.01). The activities of SOD and GSH-PX were higher and MDA level was lower in H2O2 group than those in control group. These effects of H2O2 on SOD, GSH-PX activities and MDA content were reversed by SFI in concentration-dependent way (p<0.05 and p<0.01). Flow cytometry and AO-EB double staining discovered that SFI pretreatment inhibited the ECV304 cells apoptosis. The protein expression of caspase3 in 30 μl/ml and 40 μl/ml SFI groups significantly decreased whereas Bcl2 protein expressions in 20 μl/ml, 30 μl/ml and 40 μl/ml SFI groups were higher than H2O2 group, with Bax protein expression much lower than H2O2 group (p<0.05 and p<0.01). Conclusions Our findings suggest that SFI could prevent the ECV304 cells against H2O2 oxidative-stress by enhancing antioxidant enzyme activities, reducing the membrane lipid peroxidation, as well as upregulating antiapoptotic and downregulating apoptosis protein expressions. © 2015 Elsevier Ireland Ltd. All rights reserved.
Zhang X.,Nanchang University |
Lu H.,Basic Medical College |
Wang Y.,Nanchang University |
Liu C.,Jiangxi University of Traditional Chinese Medicine |
And 4 more authors.
International Journal of Molecular Medicine | Year: 2015
Taurine (Tau), the most abundant free amino acid in humans has numerous potential health benefits through its antioxidant and anti-inflammatory properties. However, limited studies have assessed its effect on tumors and the antitumor mechanism remains unknown. The present study investigated the cellular and molecular changes induced by Tau, leading to the induction of apoptosis in human breast cancer cell lines MCF-7 and MDA-MB-231. MCF-7 is p53 profi cient (p53+/+) and MDA-MB-231 is a p53 null mutant (p53-/-). Cell proliferation and viability were assessed by MTT. Flow cytometry and hoechst33342 fluorescent staining were employed to detect apoptosis. Spectrophotometry was used to detect caspase-3 activity. Reverse transcription-polymerase chain reaction and western blot analysis were used to detect the levels of mRNA and proteins of p53-upregulated modulator of apoptosis (PUMA), Bax and Bcl-2. Finally, the affect of Tau on the growth of MDA-MB-231-cell-nude mice xenografts was examined. In the study, Tau inhibited growth and induced apoptosis of the two cell lines in a concentration- and time-dependent manner. Notably, the inhibitory effect of Tau on p53-/- cancer cells was clearly significant compared to the p53+/+ cancer cells. Further studies showed that Tau promoted apoptosis in human breast cancer cells and inhibited the growth of tumor in nude mice by inducing the expression of PUMA, which further up- and downregulated the expression of Bax and Bcl-2 protein, giving rise to increased activation of caspase-3. Collectively, these results indicate that Tau is a potent candidate for the chemotherapy of breast cancer through increasing the PUMA expression independent of p53 status.
Wan H.-F.,Nanchang University |
Yu L.-H.,Nanchang University |
Wu J.-L.,Nanchang University |
Tu S.,Basic Medical College |
And 4 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2013
Aim: To investigate the effects of diallyl trisulfide (DT) on apoptosis of cisplatin (DDP)-resistant human epithelial ovarian cancer SKOV-3 cells (SKOV-3/DDP), and the role of p53 upregulated modulator of apoptosis (PUMA). Methods: SKOV-3/DDP cells were randomly divided into control, DT, DPP and DPP+DT groups, which were treated with DT or combined DT and DDP. All cells were incubated for 48 h. and apoptosis rates were assessed by flow cytometry. mRNA and protein expression of PUMA, Bax and Bcl-2 was determined by RT-PCR and Western blot assays, respectively. Results: Compared with control group, the apoptosis rates of SKOV-3/DDP cells in DT groups were obviously increased, with dose-dependence (P < 0.05), the mRNA and protein expressions of PUMA, Bax also being up-regulated (P < 0.05), while those of Bcl-2 were down-regulated (P < 0.05). Compared with DT groups, the apoptosis rate in the DDP+DT group was significantly increased (P < 0.05). After knockdown of PUMA with specific siRNA, the apoptosis rate of SKOV-3/DDP cells was obviously decreased (P < 0.05). Conclusion: DT can promote the apoptosis of SKOV-3/DDP cells with PUMA playing a critical role.
Li N.,Basic Medical College |
Zhang B.,The First Affiliated Hospital
World Chinese Journal of Digestology | Year: 2015
AIM: To observe the clinical effects of Chinese medicine rectal instillation therapy combined with Western medicine in treating cholestatic infancy hepatitis syndrome and its effect on blood biochemical parameters. METHODS: Eighty-three patients diagnosed with cholestatic infancy hepatitis syndrome were randomized into either a treatment group (n = 42) or a control group (n = 41). The control group was treated with Western medicine alone, and the treatment group was given traditional Chinese medicine rectal instillation therapy combined with Western medicine. The treatment lasted 6 weeks. After treatment, serum total bilirubin (TBIL), direct bilirubin (DBIL), alkaline phosphatase (ALP), alanine aminotransferase (ALT), total bile acid (TBA), gamma-glutamyl transpeptidase (GGT), CMVIgM negative conversion rate, and liver size were compared between the two groups. RESULTS: The effective rate and CMV-IgM negative conversion rate were significantly higher in the treatment group than in the control group (P < 0.05). After 6 wk of treatment, the levels of ALT, TBA, ALP, TBIL, DBIL and GGT in both groups improved significantly (P < 0.05), and were dramatically lower in the treatment group than in the control group (P < 0.05). The liver size in the treatment group was significantly decreased compared with the control group (P < 0.05). The time to jaundice disappearance was significantly shorter in the treatment group than in the control group (P < 0.05). CONCLUSION: Chinese medicine rectal instillation therapy combined with Western medicine can reduce the levels of ALT, TBA, ALP, TBIL, DBIL and GGT, and improve the anti-CMV-IgM negative conversion rate in patients with cholestatic infant hepatitis syndrome. © 2015 Baishideng Publishing Group Inc. All rights reserved.
Huang L.-L.,Anhui Medical University |
Huang L.-L.,CAS Hefei Institutes of Physical Science |
Pan C.,Lishui Peoples Hospital |
Wang L.,Anhui Medical University |
And 6 more authors.
Journal of Nutritional Biochemistry | Year: 2015
Cardiovascular remodeling, as a hallmark of hypertension-induced pathophysiology, causes substantial cardiovascular morbidity and mortality. There is increasing evidence that has demonstrated a broad spectrum of pharmacological and therapeutic benefits of grape seed proanthocyanidins (GSP) against oxidative stress and cardiovascular diseases. In this study, 180- to 200-g SD rats treated with DOCA (120 mg/week sc with 1% NaCl and 0.2% KCl in drinking water) and GSP (150, 240, 384 mg/kg) or amlodipine (ALM) (5 mg/kg) for 4 weeks were recruited. The protective effects of GSP on blood pressure and cardiovascular remodeling in rats with DOCA-salt-induced hypertension were investigated. Our results indicated that DOCA-salt could induce hypertension, cardiovascular remodeling and dysfunction, oxidative stress and the release of endothelin-1 (ET-1) and could increase JNK1/2 and p38MAPK phosphorylation. GSP or ALM treatments significantly improved hypertension, cardiovascular remodeling and dysfunction and oxidative stress, restrained the release of ET-1 and down-regulated the JNK1/2 and p38MAPK phosphorylation. These findings demonstrate that GSP has protective effects against increase of blood pressure induced by DOCA-salt hypertension and cardiovascular remodeling by inhibiting the reactive oxygen species/mitogen-activated protein kinase pathway via restraining the release of ET-1. © 2015 Elsevier Inc.
Zhang Z.,Tianjin Medical University |
Zhang Z.,Basic Medical College |
Huang B.,Tianjin Medical University |
Gao F.,CAS Institute of Zoology |
And 2 more authors.
Current Stem Cell Research and Therapy | Year: 2015
It has been demonstrated that mouse and human somatic cells can be reprogrammed into an embryonic stem cell-like state by introducing combinations of the transcription factors. The generation of such induced pluripotent stem cells (iPSCs) has enabled the derivation of disease-specific pluripotent cells which opens up new avenues of disease modeling and provides valuable experimental platforms. Moreover, technologies for creating humanized animal models by human iPSCs will be available as well, which will increase the utility of humanized mice for research. Emerging evidences suggest, however, that immunogenicity of iPSCs seems to be a vital and controversial issue surrounding potential of iPSCs. Recent studies on induced multipotent progenitor cells (iMPCs) extend the applications of iPSC technology and provide promising candidates for disease modeling. In this review, we introduce a wide range of applications of iPSCs in disease modeling and discuss the immune response on the use of iPSCs as well as a promising alternative for future directions of disease modeling. © 2015, Bentham Science Publishers.
Su W.,Basic Medical College |
Liu X.,China Medical University at Heping
Journal of Molecular Endocrinology | Year: 2013
In mammalian testes, the blood-testis barrier (BTB), created by specialized junctions between Sertoli cells near the basement membrane of the seminiferous epithelium, provides an indispensable immune-privileged microenvironment for spermatid development. However, the BTB must experience restructuring during the epithelial cycle to facilitate the transit of preleptotene spermatocytes upon the testosterone-induced new TJ fibrils forming behind these cells, which is intimately related to the extensive dynamics of junction protein complexes between Sertoli cells. As key regulators of protein traffic, Rab GTPases participate in delivery of proteins between distinct cellular sites and cross talk with proteins that constitute tight junction and adherens junction. Using primarily cultured Sertoli cells in vitro with an established tight junction permeability barrier that mimics the BTB in vivo, RAB13 was shown to decrease during the testosterone-induced TJ integrity enhancement, accompanied with an increment in protein kinase A (PKA) activity. Furthermore, knockdown of Rab13 was found to resemble the effect of testosterone on Sertoli cell TJ permeability by reinforcing filamentous actin and occludin distribution at the cell-cell interface and promoting the direct interaction between ZO-1 and occludin. Interestingly, the effects of testosterone and Rab13 knockdown on Sertoli cell epithelium were revealed to be antagonized by PKA activity inhibition. In summary, RAB13 serves as a regulatory component in the assembly and restructuring of the TJ fibrils between adjacent Sertoli cells. © 2013 Society for Endocrinology Printed in Great Britain.