Basic and Supporting Discipline Unit

Hisār, India

Basic and Supporting Discipline Unit

Hisār, India
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Nayan V.,National Dairy Research Institute | Nayan V.,Central Institute for Research on Buffaloes ICAR | Bhardwaj A.,National Dairy Research Institute | Bhardwaj A.,Basic and Supporting Discipline Unit | Singh D.,National Dairy Research Institute
Agricultural Research | Year: 2013

The insulin-like growth factor binding proteins and pregnancy-associated plasma protein-A (PAPP-A) within the future dominant follicle is a critical part of selection of one follicle. This study was aimed to find out the expression and hormonal regulation of PAPP-A mRNAs in the Indian water buffalo ovary. The results showed stage-specific expression of PAPP-A in the whole follicles and postovulatory structures, corpus luteum and corpus albicans. Expression of PAPP-A mRNA was found to be up regulated in corpus luteum. The 341 nucleotide partial cDNA sequence of buffalo PAPP-A was determined. Sequence analyses have revealed that bubaline PAPP-A belongs to Zinc-metzincin superfamily (zinc-dependent metalloprotease) and pappalysin-like subfamily. It also confirmed the stimulatory effect of follicle-stimulating hormone on PAPP-A mRNA expression. The PAPP-A sequences showed more than 90 % homology with reported sequences of cattle, sheep, pig, and human. The study was an attempt in understanding local regulatory mechanism of follicular selection and development in buffalo ovary. © 2013 NAAS (National Academy of Agricultural Sciences).


Bhardwaj A.,Basic and Supporting Discipline Unit | Nayan V.,Central Institute for Research on Buffaloes | Parvati,Basic and Supporting Discipline Unit | Mamta,Basic and Supporting Discipline Unit | Gupta A.K.,Basic and Supporting Discipline Unit
Asian Journal of Animal and Veterinary Advances | Year: 2012

Inhibin is a non-steroidal glycoprotein hormone of gonadal origin with major action as negative feedback control of the production of Follicle Stimulating Hormone (FSH) by anterior pituitary gland which in turn modulates male and female reproductive functions. Its physiological role has led to the development of inhibin based immunogens for fertility enhancement in farm animals. It is envisaged that a reduction of endogenous inhibin secretion would increase FSH concentrations and thus offers a potential for increasing the number of ovulatory follicles in the ovary. Immunization against inhibin has been reported to be a useful method for inducing multiple ovulations in farm animals. Inhibins play important roles in the regulation of fertility based on their dual inhibitory action on the process of folliculogenesis in the ovary and FSH secretion by the pituitary. Inhibins are also recognized as paracrine ovarian and testicular regulators and have multiple paracrine effects in the utero-placental unit, representing a promising marker for male and female infertility, gynecological and gestational diseases.


Gupta A.K.,National Research Center on Equines | Gupta A.K.,Basic and Supporting Discipline Unit | Pal Y.,National Research Center on Equines | Pal Y.,Basic and Supporting Discipline Unit | Kumar S.,National Research Center on Equines
Indian Journal of Animal Sciences | Year: 2016

An equine chorionic gonadotrophin (eCG) based sandwich ELISA (sELISA) was developed for confirmation of early conception in pregnant mares around 30 days of gestation and fetus viability thereafter. This serum based quantitative assay is quite sensitive, specific, precise and efficient. Serum eCG content higher than 10 IU/ml during early gestation was used for confirmation of conception while eCG content more than 40 IU/ml was taken as cut off for fetus viability between 40-60 days of gestation. Assay was performed with more than 4,000 field serum samples and compared with equivalent commercial pregnancy diagnostic kit with its better sensitivity. Assay showed little cross reactivity with serum of non pregnant mares, jennies, and other animal species. Confirmation of fetus viability sELISA helped in re-servicing the mares which are observed to empty after conception failure or fetal loss.


Bhardwaj A.,Basic and Supporting Discipline Unit | Nayan V.,Central Institute for Research on Buffaloes CIRB | Yadav P.,National Dairy Research Institute | De S.,National Dairy Research Institute | And 2 more authors.
Animal Biotechnology | Year: 2012

Inhibin is a non-steroidal glycoprotein hormone of gonadal origin with major action as negative feedback control of the production of FSH by the anterior pituitary gland. The physiological role of inhibin has led to the development of inhibin immunogens for fertility enhancement in farm animals. It is envisaged that a reduction of endogenous inhibin secretion would increase FSH concentrations and thus offers a potential for increasing the number of ovulatory follicles in the ovary. The present work was carried out to produce recombinant bovine (Indian Sahiwal Cattle; Bos indicus) alpha inhibin (bINH-α) in E. coli by optimizing its expression and purification in biologically active form and to study its immunological characterization. A bacterial protein expression vector system based on the phage T5 promoter was used. The bINH-α encoding gene was successfully cloned and expressed in E. coli and the purified recombinant bINH-α was characterized. Recombinant bINH-α (25μgmL-1) immunized guinea pigs had a significant increase in litter size compared to the control group. These results indicate a role for recombinant bINH-α as a fecundity vaccine to enhance the ovulation rate and litter size in animals. © 2012 Copyright Taylor and Francis Group, LLC.


PubMed | Basic and Supporting Discipline Unit
Type: Journal Article | Journal: Animal biotechnology | Year: 2012

Inhibin is a non-steroidal glycoprotein hormone of gonadal origin with major action as negative feedback control of the production of FSH by the anterior pituitary gland. The physiological role of inhibin has led to the development of inhibin immunogens for fertility enhancement in farm animals. It is envisaged that a reduction of endogenous inhibin secretion would increase FSH concentrations and thus offers a potential for increasing the number of ovulatory follicles in the ovary. The present work was carried out to produce recombinant bovine (Indian Sahiwal Cattle; Bos indicus) alpha inhibin (bINH-) in E. coli by optimizing its expression and purification in biologically active form and to study its immunological characterization. A bacterial protein expression vector system based on the phage T(5) promoter was used. The bINH- encoding gene was successfully cloned and expressed in E. coli and the purified recombinant bINH- was characterized. Recombinant bINH- (25gmL(-1)) immunized guinea pigs had a significant increase in litter size compared to the control group. These results indicate a role for recombinant bINH- as a fecundity vaccine to enhance the ovulation rate and litter size in animals.

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