Bashkortostan State Medical University

Ufa, Russia

Bashkortostan State Medical University

Ufa, Russia
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Korytina G.F.,Institute of Biochemistry and Genetics | Akhmadishina L.Z.,Institute of Biochemistry and Genetics | Kochetova O.V.,Institute of Biochemistry and Genetics | Aznabaeva Y.G.,Bashkortostan State Medical University | And 2 more authors.
Molecular Biology | Year: 2017

Chronic obstructive pulmonary disease (COPD) is a complex chronic inflammatory disease of the respiratory system that affects primarily distal respiratory pathways and lung parenchyma. Smoking tobacco is a major risk factor for COPD. The relationship of HTR4 (rs3995090), HTR2A (rs6313), GRIK5 (rs8099939), GRIN2B (rs2268132), and CHRNB4 (rs1948) gene polymorphisms and COPD, as well as the contribution of these polymorphisms to the variations in quantitative characteristics that describe respiratory function, smoking behavior, and nicotine dependence was assessed in an ethnically homogeneous Tatar population. The polymorphisms of HTR2A (rs6313) (P = 0.026, OR = 1.42 for the CC genotype) and GRIN2B (rs2268132) (P = 0.0001, OR = 2.39 for the TT genotype) were significantly associated with increased risk of COPD. The AA genotype of GRIK5 (rs8099939) had a protective effect (P = 0.02, OR = 0.61). Importantly, the HTR2A (rs6313), GRIN2B (rs2268132), and GRIK5 (rs8099939) polymorphisms were only associated with COPD in smokers. Smoking index (pack-years) was significantly higher in carriers of the GRIK5 genotype AC (rs8099939) (P = 0.0027). The TT genotype of GRIN2B (rs2268132) was associated with COPD in subjects with high nicotine dependence according to the Fagerström test (P = 0.002, OR = 2.98). The TT genotype of HTR2A (rs6313) was associated with a reduced risk of the disease in the group with moderate nicotine dependence (P = 0.02, OR = 0.22). The CC genotype of HTR2A (rs6313) and the TT genotype of GRIN2B (rs2268132) were associated with higher levels of nicotine dependence according to the Fagerström test (P = 0.0011 and P = 0.037). Our results may provide insight into potential molecular mechanisms that involve the glutamate (GRIK5, GRIN2B) and serotonin (HTR2A) receptor genes in the pathogenesis of COPD. © 2017, Pleiades Publishing, Inc.


Gantsev S.K.,Bashkortostan State Medical University | Umezawa K.,Aichi Medical University | Islamgulov D.V.,Russian Academy of Sciences | Khusnutdinova E.K.,Russian Academy of Sciences | And 3 more authors.
Biomedicine and Pharmacotherapy | Year: 2013

The expression profiling analysis of inflammatory chemokines and their receptors in newly formed lymph nodes in breast cancer was carried out. The analysis revealed the increase in expression of the genes CCL16, XCR1, CYFIP2, TNFSF14 and the reduction in expression of chemokine ligands CXCL5 and CXCL12 in tertiary lymphoid organs. The obtained results allow us to suggest that the process of induction of lymph nodes neogenesis is identical (in its key mechanisms) to the process of lymphoid tissue neogenesis in autoimmune diseases and in some infections, but may have different triggers. © 2013 Elsevier Masson SAS.


Gantsev S.K.,Bashkortostan State Medical University | Ishmuratova R.S.,Bashkortostan State Medical University | Tatunov M.A.,Chelyabinsk Regional Hospital | Kzyrgalin S.R.,Bashkortostan State Medical University
Biomedicine and Pharmacotherapy | Year: 2013

The paper presents the research results of lymphoangionodulary anastomosis perfusion properties in breast cancer. We have carried out the detailed study of 100 axillary lymph nodes affected with metastatic breast cancer and removed them using the method of sonolipodestruction ex vivo. The results obtained in the course of the study provide a basis for a new look at the problems of lymphodynamics in metastatic damage of a lymph system. © 2013 Elsevier Masson SAS.


Idrisov B.,Bashkortostan State Medical University | Sun P.,University of Southern California | Akhmadeeva L.,Bashkortostan State Medical University | Arpawong T.E.,University of Southern California | And 2 more authors.
Addictive Behaviors | Year: 2013

This study evaluates the performance of the Project EX tobacco use cessation program in Russian summer recreational camps. An eight-session clinic-based tobacco use cessation program for adolescents was tested during the summer of 2011 in an experimental pilot trial that involved different youth that rotated through camps. Conditions were nested within camps. Two rotations of unique subject groups of smokers (program and standard care control) through each of five camps provided the means of controlling for campsite by condition. Assignment of condition by rotation was random (by a flip of a coin), achieving reasonable baseline comparability (total n. =. 164 smokers at baseline, 76 program group, 88 standard care control group). Evaluation involved an immediate pretest and posttest and a six-month telephone follow-up. At immediate posttest, Project EX was moderately well-received, significantly reduced future smoking expectation (46% reduction in EX program condition versus 8% in control, p. <0001), decreased intention to not quit smoking (-5.2% in EX versus +. 1.4% in control, p. <.05), and increased motivation to quit smoking (0.72 versus -0.04, p. <.0001). At the six-month follow-up, program subjects reported a higher intent-to-treat quit rate during the last 30. days (7.5% versus 0.1%, p. <05). For the subjects who remained monthly smokers at the six-month follow-up, Project EX reduced subjects' level of nicotine dependence (-0.53 versus +0.15, p<001). The results were quite promising for this program, which included motivation enhancement, coping skill, and alternative medicine material. However, further research on teen tobacco use cessation programming in Russia with larger sample sizes, involving other locations of the country, and with stronger research designs is needed. We evaluated a Project EX tobacco use cessation program in Russian summer camps. The experimental pilot trial among teens included 3 data collection points (n=164). At immediate posttest, Project EX increased motivation to quit smoking. At 6-months, higher quit rates were found in the intervention vs. control group. At 6-months, reduced nicotine dependence was found in intervention vs. control. © 2013 Elsevier Ltd.


Korytina G.F.,Institute of Biochemistry and Genetics | Akhmadishina L.Z.,Institute of Biochemistry and Genetics | Viktorova E.V.,University of Gottingen | Tselousova O.S.,Institute of Biochemistry and Genetics | And 3 more authors.
Journal of Human Genetics | Year: 2013

We investigated the association of matrix metalloproteinases, the disintegrin and metalloprotease 33 and the tissue and serum inhibitors of proteinase gene polymorphisms with severe chronic respiratory diseases in Tatar children. We analyzed the case-control data sample from a total of 592 Tatar individuals, consisting of 119 children with chronic bronchitis, 138 with recurrent pneumonia and 335 control children residing in Ufa (Russia). The percentage of heterozygous genotype for the MMP9 (2660A>G) was higher among healthy children (52.54% vs 36.13% in chronic bronchitis patients, P adj =0.0033, P cor =0.033, odds ratio (OR)=0.51; and 36.96% in recurrent pneumonia group, P adj =0.0034, P cor =0.034, OR=0.53). The MMP12 (-82A>G) locus was associated with chronic bronchitis in the additive model (P adj =0.0091, P cor =0.09, OR=0.45, β=-0.798). The relationship between the 6A6A genotype of MMP3 (-1171 5A>6A) (P adj =0.0013, P cor =0.013, OR=3.91) and the 6A-A haplotype of MMP3 (-1171 5A>6A) and MMP12 (-82A>G) and recurrent pneumonia were unraveled (P adj =0.001, P cor =0.01, OR=2.07). This haplotype was also associated with a higher risk of chronic bronchitis (P adj =0.0012, P cor =0.012, OR=2.15). The TIMP3 (-1296T>C) was associated with recurrent pneumonia in the dominant model (P adj =0.0031, P cor =0.031, OR=1.91). The MMP9, MMP3 and TIMP3 (tissue inhibitors of matrix metalloproteinases) polymorphisms and MMP3 and MMP12 haplotypes may play a substantial role in susceptibility to severe airway and lung injury in children with chronic bronchitis and recurrent pneumonia. © 2013 The Japan Society of Human Genetics All rights reserved.


Nijevitch A.A.,Bashkortostan State Medical University | Idrisov B.,Brandeis University | Akhmadeeva E.N.,Bashkortostan State Medical University | Graham D.Y.,Baylor College of Medicine
Current Pharmaceutical Design | Year: 2014

Helicobacter pylori is a gram-negative, microaerophilic spiral bacillus that is associated with life-threatening diseases such as gastric cancer, gastric MALT lymphoma, and peptic ulcer disease. The definition of an effective therapy is one that achieves at least a 90% eradication rate on a per-protocol basis with the first attempt. Eradication rates of H. pylori have declined to unacceptable levels worldwide, mostly due to antibiotic resistance and standard triple therapy gradually has lost its efficacy in most counties. However, bismuth quadruple therapy, when prescribed properly, has maintained its effectiveness. Alternative first-line regimens such as sequential and concomitant therapy were developed to substitute for standard triple therapy and were highly effective in the countries where they were developed, but proved susceptible to failure in regions with high rates of antibiotic resistance. Antibiotic resistance rates in Russia are high, however there is lack of data regarding comparative efficacy of first-line eradication options. The authors of this review extrapolate the knowledge of H. pylori first-line eradication options in Russia based on data from other countries, as well as from domestic studies. The available data support use of 14-day regimens with concomitant therapy, bismuth quadruple therapy, or furazolidone quadruple therapy for empiric use in adults. In addition, 14-day levofloxacin-containing therapies could be used if resistance is relatively low or lacking as triple therapy or possibly as a 5-day concomitant levofloxacin therapy. © 2014 Bentham Science Publishers.


Kataev V.A.,Bashkortostan State Medical University | Meshcheryakova S.A.,Bashkortostan State Medical University | Lazarev V.V.,Ufa State Aviation Technical University | Kuznetsov V.V.,Ufa State Petroleum Technological University
Russian Journal of Organic Chemistry | Year: 2013

Reactions of 6-methylpyrimidine-2,4(1H,3H)-dione or 5-hydroxy-6-methyl- pyrimidine-2,4(1H,3H)-dione with 2-chloromethylthiirane afforded the corresponding substituted 1-(thietan-3-yl)pyrimidine-2,4(1H,3H)-diones. The calculations in the framework of approximations PBE/3z, B3LYP/6-31G++(d,p) and MP2/6-31G++(d,p) showed that the alkylation occurred at the atom N1 of the pyrimidine ring. © 2013 Pleiades Publishing, Ltd.


PubMed | Russian Academy of Sciences and Bashkortostan State Medical University
Type: Journal Article | Journal: Biochemical genetics | Year: 2016

Chronic obstructive pulmonary disease (COPD) is a complex chronic inflammatory disease of the respiratory system affecting primarily distal respiratory pathways and lung parenchyma. This work was designed as a case-control study aimed at investigating the association of COPD with polymorphisms in inflammatory and immune response genes (JAK1, JAK3, STAT1, STAT3, NFKB1, IL17A, ADIPOQ, ADIPOR1, etc.) in Tatar population from Russia. Ten SNPs (rs310216, rs3212780, rs12693591, rs2293152, rs28362491, rs4711998, rs1974226, rs1501299, rs266729, and rs12733285) were genotyped by the real-time polymerase chain reaction (TaqMan assays) in a case-control study (425 COPD patients and 457 in the control group, from Ufa, Russia). Logistic regression was used to detect the association of SNPs in different models. Linear regression analyses were performed to estimate the relationship between SNPs and lung function parameters and pack-years. In Tatar population, significant associations of JAK1 (rs310216) (P=0.0002, OR 1.70 in additive model), JAK3 (rs3212780) (P=0.001, OR 1.61 in dominant model), and IL17A (rs1974226) (P=0.0037, OR 2.31 in recessive model) with COPD were revealed. The disease risk was higher in carriers of insertion allele of NFKB1 (rs28362491) (P=0.045, OR 1.22). We found a significant gene-by-environment interaction of smoking status and IL17A (rs1974226) (P interact=0.016), JAK3 (rs3212780) (P interact=0.031), ADIPOQ (rs266729) (P interact=0.013), and ADIPOR1 (rs12733285) (P interact=0.018). The relationship between the rs4711998, rs1974226, rs310216, rs3212780, rs28362491, and smoking pack-years was found (P=0.045, P=0.004, P=0.0005, P=0.021, and P=0.042). A significant genotype-dependent variation of forced vital capacity was observed for NFKB1 (rs28362491) (P=0.017), ADIPOR1 (rs12733285) (P=0.043), and STAT1 (rs12693591) (P=0.048). The genotypes of STAT1 (rs12693591) (P=0.013) and JAK1 (rs310216) (P=0.048) were associated with forced expiratory volume in 1s.


PubMed | Bashkortostan State Medical University
Type: Journal Article | Journal: Current pharmaceutical design | Year: 2014

Helicobacter pylori is a gram-negative, microaerophilic spiral bacillus that is associated with life-threatening diseases such as gastric cancer, gastric MALT lymphoma, and peptic ulcer disease. The definition of an effective therapy is one that achieves at least a 90% eradication rate on a per-protocol basis with the first attempt. Eradication rates of H. pylori have declined to unacceptable levels worldwide, mostly due to antibiotic resistance and standard triple therapy gradually has lost its efficacy in most counties. However, bismuth quadruple therapy, when prescribed properly, has maintained its effectiveness. Alternative first-line regimens such as sequential and concomitant therapy were developed to substitute for standard triple therapy and were highly effective in the countries where they were developed, but proved susceptible to failure in regions with high rates of antibiotic resistance. Antibiotic resistance rates in Russia are high, however there is lack of data regarding comparative efficacy of first-line eradication options. The authors of this review extrapolate the knowledge of H. pylori first-line eradication options in Russia based on data from other countries, as well as from domestic studies. The available data support use of 14-day regimens with concomitant therapy, bismuth quadruple therapy, or furazolidone quadruple therapy for empiric use in adults. In addition, 14-day levofloxacin-containing therapies could be used if resistance is relatively low or lacking as triple therapy or possibly as a 5-day concomitant levofloxacin therapy.


PubMed | Bashkortostan State Medical University
Type: Journal Article | Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Year: 2013

The expression profiling analysis of inflammatory chemokines and their receptors in newly formed lymph nodes in breast cancer was carried out. The analysis revealed the increase in expression of the genes CCL16, XCR1, CYFIP2, TNFSF14 and the reduction in expression of chemokine ligands CXCL5 and CXCL12 in tertiary lymphoid organs. The obtained results allow us to suggest that the process of induction of lymph nodes neogenesis is identical (in its key mechanisms) to the process of lymphoid tissue neogenesis in autoimmune diseases and in some infections, but may have different triggers.

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