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Karnataka, India

Arundhathi S.,Sree Siddhartha Medical College | Sunitha S.,Basaweshwara Medical College
International Journal of Pharma and Bio Sciences

Diagnosis by direct immunofluorescence (DIF) requires skin biopsies with preserved immunoreactants for which biopsies are snap-frozen in liquid nitrogen or placed in Michel's fixative that facilitates transport. Retrospective study was conducted on 30 perilesional skin biopsies of immune- vesciculobullous lesions in Michel's medium to analyse its efficiency as transport medium. DIF was performed and samples were grouped according to the number of days they had been maintained in Michel's medium. DIF was positive in 28 cases, accounting for 93.33%. DIF was positive in 100%, 92.31% and 83.33% of biopsies even after 5, 10 and 15 days of preservation in Michel's medium, respectively. As the number of days in transport medium increased, the intensity of immunofluorescence decreased. There was no statistical significance (>0.05) between the intensity of immunofluorescence and the number of days the biopsies were in transport medium. Michel's medium is an efficient transport medium, when used within 15 days, in countries where DIF facilities are not available. Source

Venkatesh V.N.,Basaweshwara Medical College | Prashanth H.V.,Sri Siddhartha Medical College | Bhat K.G.,Maratha Mandal Dental College | Subha D.S.,Basaweshwara Medical College | And 2 more authors.
Journal of Clinical and Diagnostic Research

Background: Rotavirus infects almost all children by the age of fve. More than 150,000 annual deaths due to rota virus, occurs in India. Aims: This study aimed to determine the incidence of Rotavirus infection in children and compare enzyme - linked immunosorbent assay (ELISA) and a modifed polyacrylamide gel elctrophoretic (PAGE) analysis in detection of rota virus in stool samples. Materials and Methods: In this prospective study, a total of 200 stool samples were examined for the presence of Rota virus by ELISA and by a modifed PAGE analysis of viral genome. Stool culture was done for common enteric pathogens. Results: Maximum incidence of rotavirus infection was seen in age group of 6m-24 m(32.3%). Excellent correlation of ELISA and PAGE results was found in194 of 200 (97%) specimens. A total of 51 (25.5%) of them were found to be positive for rotavirus by either methods. The proportion of ELISA +ve PAGE -ve samples 1/200 was lower than the proportion of ELISA-ve PAGE +ve samples (5/200). All 51 rotavirus positive cases did not show infection with bacterial pathogens. Conclusion: The modifed PAGE technique for the detection of viral RNA was found to be rapid, simple, reliable and less expensive technique. Source

Sreenivasa B.N.,Basaweshwara Medical College | Kumar G.V.,Sri Siddhartha Medical College | Manjunatha B.N.,Basaweshwara Medical College
Journal of Nepal Paediatric Society

Introduction: The world health organization estimates that anaemia largely caused by iron deficiency, affecting between 500 million and two billion people worldwide. Considering the age prevalence of iron deficiency anaemia and febrile convulsion which are the same, the role of iron in the metabolism of neurotransmitter and some enzymes, the function of hemoglobin in conveying oxygen to the brain and since fever can exacerbate symptoms that result from anaemia, a relationship between iron deficiency anaemia and febrile convulsions is probable. Some studies have suggested iron deficiency as a predisposing factor for febrile seizures. We designed this case control study to evaluate the relationship between iron deficiency anaemia and febrile convulsions. The objective of this study was to study the role of iron deficiency as a risk factor for febrile seizures. Materials and Methods: Hundred cases and 100 controls were included in the study. Cases were children of age group six months to six years presenting with febrile seizures. Controls were children of same age group presenting with febrile illness but without any seizures. After informed consent, detailed history was taken and clinical examination done in both cases and controls and blood investigations were done to diagnose iron deficiency anemia. Two groups were matched for age and sex .In all children hemoglobin (Hb) level, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), red cell distribution width (RDW) and plasma ferritin (PF) were determined and the data collected were analyzed statistically. Results: The mean PF was significantly lower in cases compared to controls (p=0.000) and RDW was significantly higher in cases compared to controls (p=0.00) .The mean Hb%, MCV, MCH are lower in cases compared to controls but it was statistically not significant. Concluson: Iron deficiency is a significant risk factor for febrile seizures in children of age group six months to six years. Early detection and timely correction of iron deficiency may be helpful for prevention of febrile seizures in children. © 2015, Nepal Paediatric Society (NEPAS). All rights reserved. Source

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