Barij Essence Pharmaceutical Company Research Center

Kāshān, Iran

Barij Essence Pharmaceutical Company Research Center

Kāshān, Iran

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Haghi G.,Phytochemistry Group | Haghi G.,Barij Essence Pharmaceutical Company Research Center | Hatami A.,Phytochemistry Group | Hatami A.,Barij Essence Pharmaceutical Company Research Center | Mehran M.,Barij Essence Pharmaceutical Company Research Center
Journal of Apicultural Science | Year: 2013

A reverse-phase high-performance liquid chromatographic (RP -HPLC) method was developed and validated for the analysis of honeybee venom samples and drug products containing honeybee venom. The validation parameters were linearity, sensitivity, precision, and recovery. Melittin is the main component of honeybee venom was extracted with pure water, and then evaluated by RP -HPLC with a photodiode array (PDA) detector. Separation of the samples was achieved on a Europa Protein C18 column with linear gradient elution of acetonitrile and 0.4% phosphoric acid at 25°C. There was a flow rate of 1 mL/min. Detection was set at 220 nm. Limits of detection (LOD) and quantification (LO Q) for melittin were 1.1 and 3.2 μg/mL, respectively. The amount of melittin in honeybee venom samples ranged from 21.9 to 66.4 %.


Haghi G.,Phytochemistry Group | Haghi G.,Barij Essence Pharmaceutical Company Research Center | Hatami A.,Phytochemistry Group | Hatami A.,Barij Essence Pharmaceutical Company Research Center | Mehran M.,Barij Essence Pharmaceutical Company Research Center
Food Chemistry | Year: 2013

Analytical methods including ultra-performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC) with photodiode array (PDA) detector were developed for the analysis of caffeoylquinic acid derivatives in seeds, leaves and roots of Arctium lappa L. Separation was performed on C 18 column utilising 5% (v/v) acetic acid in water and acetonitrile at 330 nm. Both methodologies were validated in terms of linearity, precision, and recovery. The results showed that the major advantages of UPLC, over HPLC were the fast analysis, narrow peaks, high sensitivity, and reduction of solvent consumption. Subsequently the methods were applied for the identification and quantification of chlorogenic acid (5-CQA) and 1,5-dicaffeoylquinic acid (1,5-DCQA) as main compounds in samples. The total phenolic content of samples ranged from 3.93 to 14.13 g of 5-CQA equivalent/100 g dry weight (DW). There was a significant variability from 89 to 571 mg/100 g for 5-CQA and 48 to 486 mg/100 g for 1,5-DCQA in dry material. © 2012 Elsevier Ltd. All rights reserved.


Haghi G.,Phytochemistry Group | Haghi G.,Barij Essence Pharmaceutical Company Research Center | Arshi R.,Barij Essence Pharmaceutical Company Research Center | Ghazian F.,Barij Essence Pharmaceutical Company Research Center | And 2 more authors.
Journal of Essential Oil-Bearing Plants | Year: 2012

Cichorium intybus L. commonly known as Chicory or Kasni has a long history of medicinal herbal use and is especially of great value for its tonic effect upon the liver, cardio and digestive tract. In this study, the composition of volatile oil from overground parts of C. intybus has been surveyed. Two samples of C. intybus aerial parts were collected at flowering stage in center of Iran (Kashan, Isfahan province). Volatile compounds were obtained by a two-step extraction of hydrodistillation and liquid-liquid extraction. Distillate was extracted with pentane. The organic layer was separated, dried over anhydrous sodium sulphate and concentrated to 0.5 ml under reduced pressure. The concentrated extract had a strong odor and yellow color. The separation and identification of the oil components were carried out using a combination of GC-FID and GC-MS. The analysis of the oil revealed the presence of twenty components identified in the aerial parts of this herb. The main components were carvacrol (50.1%), thymol (13.3%), cinnamic aldehyde (12.4%), camphor (4.4%), carvone (4.1%), linalool (3.9%) and α-terpineol (2.1%). © 2012, Har Krishan Bhalla & Sons.

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