Lees D.C.,French National Institute for Agricultural Research |
Lees D.C.,Natural History Museum in London |
Rougerie R.,Barcoding |
Christof Z.-L.,Zeller Lukashort |
Kristensen N.P.,Copenhagen University
Zoologica Scripta | Year: 2010
Lees, D. C., Rougerie, R., Zeller-Lukashort, C. & Kristensen, N. P. (2010). DNA mini-barcodes in taxonomic assignment: a morphologically unique new homoneurous moth clade from the Indian Himalayas described in Micropterix (Lepidoptera, Micropterigidae). -Zoologica Scripta, 39, 642-661.The first micropterigid moths recorded from the Himalayas, Micropterix cornuella sp. n. and Micropterix longicornuella sp. n. (collected, respectively, in 1935 in the Arunachel Pradesh Province and in 1874 in Darjeeling, both Northeastern India) constitute a new clade, which is unique within the family because of striking specializations of the female postabdomen: tergum VIII ventral plate forming a continuous sclerotized ring, segment IX bearing a pair of strongly sclerotized lateroventral plates, each with a prominent horn-like posterior process. Fore wing vein R unforked, all Rs veins preapical; hind wing devoid of a discrete vein R. The combination of the two first-mentioned vein characters suggests close affinity to the large Palearctic genus Micropterix (to some species of which the members of the new clade bear strong superficial resemblance). Whilst absence of the hind wing R is unknown in that genus, this specialization is not incompatible with the new clade being subordinate within it. A 136-bp fragment of Cytochrome oxidase I successfully amplified from both of the 75-year-old specimens strongly supports this generic assignment. Translated to amino acids, this DNA fragment is highly diagnostic of this genus, being identical to that of most (16 of the 26) Micropterix species studied comparatively here, 1-4 codons different from nine other species (including Micropterix wockei that in phylogenetic analyses we infer to be sister to other examined species), whilst 7-15 codons different to other amphiesmenopteran genera examined here. A dating analysis also suggests that the large clade excluding M. wockei to which M. cornuella belongs appeared <31 million years ago. These findings encourage discovery of a significant radiation of Micropterix in the Himalayan region. Our analysis has more general implications for testing the assignment of DNA mini-barcodes to a taxon, in cases such as museum specimens where the full DNA barcode cannot be recovered. © 2010 The Authors. Zoologica Scripta © 2010 The Norwegian Academy of Science and Letters. Source
Barcoding | Date: 2011-12-20
A system for identifying medication in the form of pills, capsules or tablets, and communicating medicine dosage and intake instructions to a user, Utilizing Radio Frequency Identification Devices (RFID) and optical recognition technology. The RFID is performed by labeling a medicine container with a tag containing a unique identifier, associating the unique identifier with an audio file comprising instructions related to medicine usage, and delivering the audio file to an electromagnetic wave-enabled device. A wireless device, such as a mobile telephone or PDA, via a service, plays an audio and/or vibrational file associated with the unique identifier when the RFID tag is read by the device. The mobile device has a camera therein and is operable to capture an image of the pill, capsule or tablet and, via execution of optical recognition software, identify the pill, tablet or capsule, and verify the identity thereof.
Barcoding | Date: 2010-05-24
An RFID-based data collection, correlation and transmission system and method carried out thereby is provided. The system, which comprises one or more RFID-readers, a radio frequency identification (RFID) recognition and control component. a storage device interface, a portable and/or internal data storage device in communication with the storage device interface, one or more antennas, and a configuration and command component, is operable to collect data of interest from detected RFID tags, and detect and identify system participants and data related thereto. In addition, the system is operable to correlate potential data of interest, such as product advertising information, to the detected system participants, and transmit the data of interest to the system participants via numerous methods of communication. Thus, the system provides a means of highly targeted information distribution, as well as providing user reports valuable in future planning.
News Article | August 9, 2011
You’ve heard of the CSI effect, right? It’s this wacky “syndrome” whereby we’ve watched so much CSI Miami and Law and Order that we can’t fully put our weight behind a verdict without some solid DNA evidence. I guess it’s easy to forget that we had an entire legal system sans DNA for quite a while. In any case, we’ve apparently got an itch to be a bunch of white-coated forensic scientists, which is why we’re so lucky that this crazy, and also beautiful, machine exists in the world. It’s called OpenPCR, and it’ll make science-style DIYers drool. PCR stands for polymerase chain reaction, and it’s a crucial tool for just about any type of modern molecular biology. The way it works is by amplifying a specific region of a super teency-weency strain of DNA, and after that I kind of got lost in the biological jargon, but it’s all explained here. With OpenPCR, you can do two different types of tests: DNA Sequencing and DNA Barcoding. Sequencing is where you use the PCR machine to check out some of your own genome, while Barcoding is checking out what kind of species a certain bit of DNA belongs to. If you have yet to be convinced, just check out how these two girls used DNA Barcoding to uncover a New York City scandal (hint: 2 out of 4 Sushi restaurants and 6 out of 10 grocery stores were selling mislabeled fish.) For $599, you’ll get all the parts to the machine, instructions to set it up, and 16 PCR samples — the way by which you target certain regions of the DNA. Features include a heated lid that eliminates condensation, 2-degree per second ramp time (Centigrade), and compatibility with Mac and PC.
Richard B.,CNRS Biodiversity Studies Laboratory |
Decaens T.,CNRS Biodiversity Studies Laboratory |
Rougerie R.,Barcoding |
James S.W.,University of Kansas |
And 2 more authors.
Molecular Ecology Resources | Year: 2010
Species identification of earthworms is usually achieved by careful observation of morphological features, often sexual characters only present in adult specimens. Consequently, juveniles or cocoons are often impossible to identify, creating a possible bias in studies that aim to document species richness and abundance. DNA barcoding, the use of a short standardized DNA fragment for species identification, is a promising approach for species discrimination. When a reference library is available, DNA-based identification is possible for all life stages. In this study, we show that DNA barcoding is an unrivaled tool for high volume identification of juvenile earthworms. To illustrate this advance, we generated DNA barcodes for specimens of Lumbricus collected from three temperate grasslands in western France. The analysis of genetic distances between individuals shows that juvenile sequences unequivocally match DNA barcode clusters of previously identified adult specimens, demonstrating the potential of DNA barcoding to provide exhaustive specimen identification for soil ecological research. © 2009 Blackwell Publishing Ltd. Source