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Li Y.,Peking University | Li X.,Shenyang University | Zhao H.,PLA Fourth Military Medical University | Feng R.,Peking University | And 6 more authors.
Stem Cells and Development | Year: 2013

The technology to reprogram human somatic cells back to pluripotency allows the production of patient-specific induced pluripotent stem cells (iPSCs) and holds a great promise for regenerative medicine. Choosing the most suitable cell type for induction and reducing the risk of viral transgene activation, especially oncogene activation, are important for iPSC research. To date, human dermal fibroblasts (HDFs) are the most frequent cell source used for iPSC generation, but they have several limitations. An invasive skin biopsy must be performed to obtain HDFs, and HDFs must be cultured for a prolonged period before they can be used for experiments. Thus, in an effort to develop a suitable source for iPSC studies to avoid the limitations mentioned above, we have here identified stromal cells derived from menstrual blood (MenSCs) as suitable candidates. In the present study, we found that MenSCs can be reprogrammed to pluripotent status by doxycycline-inducible lentiviral transduction of OCT4, SOX2, and KLF4. Additionally, we found that MenSCs have a significantly higher reprogramming efficiency than HDFs. The combination of OCT4 and SOX2 is sufficient to reprogram MenSCs into iPSCs without the use of c-MYC or KLF4. The resulting MenSC-iPSCs showed the same characteristics as human embryonic stem cells with regard to morphology, pluripotent markers, gene expression, and the epigenetic status of pluripotent-cell-specific genes. These cells were able to differentiate into various cell types of all 3 germ layers both in vitro and in vivo. Therefore, MenSCs may be a preferred candidate for generation of iPSCs. © Copyright 2013, Mary Ann Liebert, Inc. 2013.


Dong W.,CAS Institute of Botany | Cheng T.,CAS Institute of Botany | Cheng T.,University of Chinese Academy of Sciences | Li C.,CAS Institute of Botany | And 6 more authors.
Molecular Ecology Resources | Year: 2014

The ideal DNA barcode for plants remains to be discovered, and the candidate barcode rbcL has been met with considerable skepticism since its proposal. In fact, the variability within this gene has never been fully explored across all plant groups from algae to flowering plants, and its performance as a barcode has not been adequately tested. By analysing all of the rbcL sequences currently available in GenBank, we attempted to determine how well a region of rbcL performs as a barcode in species discrimination. We found that the rbcLb region was more variable than the frequently used rbcLa region. Both universal and plant group-specific primers were designed to amplify rbcLb, and the performance of rbcLa and rbcLb was tested in several ways. Using blast, both regions successfully identified all families and nearly all genera; however, the successful species identification rates varied significantly among plant groups, ranging from 24.58% to 85.50% for rbcLa and from 36.67% to 90.89% for rbcLb. Successful species discrimination ranged from 5.19% to 96.33% for rbcLa and from 22.09% to 98.43% for rbcLb in species-rich families, and from 0 to 88.73% for rbcLa and from 2.04% to 100% for rbcLb in species-rich genera. Both regions performed better for lower plants than for higher plants, although rbcLb performed significantly better than rbcLa overall, particularly for angiosperms. Considering the applicability across plants, easy and unambiguous alignment, high primer universality, high sequence quality and high species discrimination power for lower plants, we suggest rbcLb as a universal plant barcode. © 2013 John Wiley & Sons Ltd.


Li M.-H.,Peking Union Medical College | Li M.-H.,Baotou Medical College | Peng Y.,Peking Union Medical College | Xiao P.-G.,Peking Union Medical College
Journal of Systematics and Evolution | Year: 2010

Salvia L. (family Lamiaceae), a large genus of over 1000 species, is widely distributed throughout tropical and temperate regions of the world. Eight-four species are native to China. However, there are many taxonomic uncertainties at the sub-generic level. Diterpenoids are a class of secondary metabolites with a large variety of structures that have been used as chemotaxonomic markers at infra-and suprageneric levels. For the sake of further chemotaxonomic understanding of Salvia, the present study investigated the distribution of tanshinones, a group of biologically active diterpenes known to be present in some Chinese members of the genus. Using high-pressure liquid chromatography with a diode array detector, tanshinones in various Salvia species were determined and the distribution supported the circumscription of sect. Drymosphare in the original sense of Bentham. Therefore, the distribution of tanshinones provides a valuable chemotaxonomic tool for determining infrageneric differences within Salvia. © 2010 Institute of Botany, Chinese Academy of Sciences.


Li F.,Shenyang University | Li F.,Baotou Medical College | Xuan J.,Shenyang University | Xing J.,Shenyang University | And 3 more authors.
Forensic Science International: Genetics | Year: 2014

Several commercial multiplex PCR kits for the amplification of short tandem repeat (STR) loci have been extensively applied in forensic genetics. Consequently, large numbers of samples have been genotyped, and the number of discordant genotypes observed has also increased. We observed allele dropout with two novel alleles at the STR loci TH01 and D13S317 during paternity testing using the AmpFℓSTR® Identifiler® PCR Amplification Kit. The lost alleles reappeared when alternative PCR primer pairs were used. A sequence analysis revealed a G-to-A substitution 82 bases downstream of the last TCAT motif of the repeat region at the TH01 locus (GenBank accession: D00269) and a G-to-T substitution 90 bases upstream of the first TATC motif of the repeat region at the D13S317 locus (GenBank accession: G09017). The frequencies of these two point mutations were subsequently investigated in the Chinese population using sequence-specific primer PCR (SSP-PCR), but neither of these mutations was detected in any of the samples tested. In addition, the DNA samples in which the mutations were identified were amplified to type the point mutations by SSP-PCR to determine the corresponding STR alleles at the two loci. Subsequently, the amplified PCR products with different point mutations and STR repeat numbers were directly sequenced because this strategy overcomes the appearance overlapping peaks generated by different STR alleles and accurately characterizes genotypes. Thus, our findings not only provide useful information for DNA databases and forensic identification but also establish an effective strategy for typing STR alleles with primer binding site mutations. © 2013 Elsevier B.V.


Ma B.,Nanjing Agricultural University | Ma B.,Baotou Medical College | Gao L.,Nanjing Agricultural University | Zhang H.,Henan University of Science and Technology | And 2 more authors.
Plant Cell Reports | Year: 2012

The effects of aluminum (Al) on root elongation, lipid peroxidation, hydrogen peroxide (H2O2) accumulation, antioxidant levels, antioxidant enzymatic activity, and lignin content in the roots of the Al-tolerant rice variety azucena and the Al-sensitive variety IR64 were investigated. Treatment with Al induced a greater decrease in root elongation and a greater increase in H2O2 and lipid peroxidation as determined by the total thiobarbituric acid-reactive substance (TBARS) level in IR64 than in azucena. Azucena had significantly higher levels of superoxide dismutase, ascorbate peroxidase, glutathione reductase, and glutathione peroxidase GSH POD activity compared with IR64. The concentrations of reduced glutathione (GSH) and ascorbic acid, and the GSH/GSSG ratio (reduced vs. oxidized glutathione) were also higher in azucena than in IR64 in the presence of Al. The addition of 1 mg/L GSH improved root elongation in both varieties and decreased H2O2 production under Al stress. By contrast, treatment with buthionine sulfoximine, a specific inhibitor of GSH synthesis, decreased root elongation in azucena and stimulated H2O2 production in both varieties. Moreover, Al treatment significantly increased the cytoplasmic activity of peroxidase (POD) as well as the levels of POD bound ionically and covalently to cell walls in the Al-sensitive variety. The lignin content was also increased. Treatment with exogenous H2O2 also increased the lignin content and decreased root elongation in IR64. These results suggest that Al induces lignification in the roots of Al-sensitive rice varieties, probably through an increase in H2O2 accumulation. © 2011 Springer-Verlag.


Yan Y.,Baotou Medical College
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases | Year: 2010

Water extract of Pulsatilla chinensis (PWE) (0.625 mg/ml) was added to the medium for the culture of Trichomonas vaginalis. After 2 h and 4 h treatment, the content and component of soluble proteins in the parasites were analyzed by SDS-PAGE. A total of 28 protein bands was demonstrated. After treated with PWE for 2 h, the content of 5 protein bands increased and that of 4 bands decreased significantly. Four hours later, a new protein band appeared in the electrophoretogram, and the content of 5 bands increased and that of other 5 bands decreased.


Yufu W.,Baotou Medical College
RISTI - Revista Iberica de Sistemas e Tecnologias de Informacao | Year: 2015

Objective: In order to improve the performance of the wrestling athletes in our country. Method: Establishing a research method of the wrestling winning laws based on logical reasoning and data statistics. Process: The article introduced the development of wrestling and related concepts, established the fuzzy logic reasoning model, and analyzed the technical and tactical characteristics of the classical style wrestling. Result &Analysis: This paper studies the various skills in wrestling, carries on statistics and analysis of a large number of wrestling sports records data, and finds that the establishment of the research method can get the general rule of wrestling. Result: the research method based on logical reasoning and data statistics is effective and feasible.


Yang R.-F.,Baotou Medical College | Du H.-Y.,Inn Mongolia Peoples Hospital
International Eye Science | Year: 2016

Retinal vein occlusion is the second most common retinal vascular disease after diabetic retinopathy. The etiology and pathogenesis is still unclear. Macular edema and retinal ischemia is the main cause of vision loss. The article illustrates the advancement of retinal vein occlusion treated with medicine, laser and surgical treatment and mentions the prospects in the future. Copyright 2016 by the IJO Press.


Wang X.,Baotou Medical College | Bai L.,Baotou Medical College | Ma Y.,Baotou Medical College | Ren Y.,Baotou Medical College | Cong M.,Baotou Medical College
Cancer Research and Clinic | Year: 2015

C-myb is a member of the myb transcription factor family and involved in cell proliferation and differentiation. It activates specific target genes to regulate specific program through certain channel, and it takes signal transduction to regulate function and expression of protein. In this paper, c-myb, by binding to Wnt signaling pathway, makes Lgr5 start to promote cell proliferation and differentiation, and it can serve as a new therapeutic target to provide the basis of early diagnosis and treatment for colorectal cancer.


Wang Y.,Baotou Medical College | Wang Z.,Baotou Medical College | Zhang Y.,Baotou Medical College | Bai L.,Baotou Medical College | And 4 more authors.
Annals of Clinical Microbiology and Antimicrobials | Year: 2014

Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification, which is commonly used to diagnose infectious diseases. The use of PCR for pathogens detection, genotyping and quantification has some advantages, such as high sensitivity, high specificity, reproducibility and technical ease. Brucellosis is a common zoonosis caused by Brucella spp., which still remains as a major health problem in many developing countries around the world. The direct culture and immunohistochemistry can be used for detecting infection with Brucella spp. However, PCR has the potential to address limitations of these methods. PCR are now one of the most useful assays for the diagnosis in human brucellosis. The aim of this review was to summarize the main PCR techniques and their applications for diagnosis and follow-up of patients with brucellosis. Moreover, advantages or limitation of the different PCR methods as well as the evaluation of PCR results for treatment and follow-up of human brucellosis were also discussed. © 2014 Wang et al.; licensee Biomed Central Ltd.

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