Baotou Medical College
Baotou Medical College
Li F.,Liaoning Medical University |
Li F.,Baotou Medical College |
Tian X.,Liaoning Medical University |
Zhou Y.,Liaoning Medical University |
And 4 more authors.
Journal of Neuroscience Research | Year: 2012
The neurotoxins paraquat (PQ) and dopamine (DA or 6-OHDA) cause apoptosis of dopaminergic neurons in the substantia nigra pars compacta (SNpc), reproducing an important pathological feature of Parkinson's disease (PD). Secretogranin III (SCG3), a member of the multifunctional granin family, plays a key role in neurotransmitter storage and transport and in secretory granule biogenesis, which involves the uptake of exogenous toxins and endogenous "toxins" in neuroendocrine cells. However, the molecular mechanisms of neurotoxin-induced apoptosis in dopaminergic neurons and the role of SCG3-associated signaling pathways in neuroendocrine regulation are unclear. To address this, we used PQ- and DA-induced apoptosis in SH-SY5Y human dopaminergic cells as an in vitro model to investigate the association between SCG3 expression level and apoptosis. SCG3 was highly expressed in SH-SY5Y cells, and SCG3 mRNA and protein levels were dramatically decreased after PQ treatment. Apoptosis induced by PQ is associated with caspase activation and decreased SCG3 expression, and restoration of SCG3 expression is observed after treatment with caspase inhibitors. Overexpressed SCG3 in nonneuronal cells and endogenous SCG3 in SH-SY5Y cells are cleaved into specific fragments by recombinant caspase-3 and -7, but the fragments were not detected in PQ-treated SH-SY5Y cells. Therefore, SCG3 may be involved in apoptosis signal transduction as a caspase substrate, leading to loss of its original biological functions. In addition, SCG3 may be a pivotal component of the neuroendocrine pathway and play an important role in neuronal communication and neurotransmitter release, possibly representing a new potential target in the course of PD pathogenesis. © 2012 Wiley Periodicals, Inc.
Yue M.,Baotou Medical College |
Wu H.G.,Zhengzhou University of Light Industry
International Journal of Grid and Distributed Computing | Year: 2016
Mobile Internet is a mainstream access and communication technology, due to access to Internet anytime and anywhere, the business will varied, and bring mass data, but the data processing has different characteristics, the delay and energy consumption are also different. Therefore, it is necessary to apply to different data mining methods in the cloud platform, so as to adapt to different business applications, this paper proposes an improved Apriori algorithm, theory and simulation can prove that the method is effective. © 2016 SERSC.
Wu B.,Zhejiang University |
Wu B.,Leibniz Institute of Marine Science |
Wu X.,Zhejiang University |
Sun M.,Leibniz Institute of Marine Science |
Li M.,Baotou Medical College
Marine Drugs | Year: 2013
Two new sesquiterpenes, 1β,5α,6α,14-tetraacetoxy-9α- benzoyloxy-7β H-eudesman-2β,11-diol (1) and 4α,5α- diacetoxy-9α-benzoyloxy-7βH-eudesman-1β,2β,11, 14-tetraol (2), were produced as stress metabolites in the cultured mycelia of Pestalotiopsis sp. Z233 isolated from the algae Sargassum horneri in response to abiotic stress elicitation by CuCl2. Their structures were established by spectroscopic means. New compounds 1 and 2 showed tyrosinase inhibitory activities with IC50 value of 14.8 μM and 22.3 μM. © 2013 by the authors.
Li F.,Inner Mongolia University |
Li F.,Baotou Medical College |
Ge B.,Baogang Hospital of Inner Mongolia |
Damirin A.,Inner Mongolia University
International Journal of Clinical and Experimental Pathology | Year: 2017
Background: Paraquat (PQ) is a powerful pathologic pesticide that contribute to the neurotoxicity, however, the pathogenic mechanism between them was unclear. The aims of this study were to explore the underlying mechanism of PQ-induced toxicity and then make potential contribute to such neuronal diseases therapy. Methods: Human cell line SH-SY5Y was pretreated with a set concentrations of PQ to detect the cell apoptosis and the expression of related genes and proteins. Next, pcDNA 3.1-p58ipk or si-p58ipk was transfected the PQ-induced cells to detect the cytotoxicity. Results: PQ significantly increased the cell apoptosis as well as the expression of p58ipk and CHOP, but decreased the expression of pAKT. p58ipk suppression resulted in an increase of cell apoptosis and CHOP expression, but the expression of pAKT was significantly decreased in PQ-induced SH-SY5Y cells. However, overexpressed p58ipk led to an opposite result. Conclusion: The results indicated that the expression of p58ipk was related to the toxicity level of PQ-induced cells and the mechanism between them was that p58ipk regulated the toxicity might through regulating the endoplasmic reticulum stress (ER-stress) and then regulating cell apoptosis. Further studies take emphasize on the effect of ER-stress on neuron system and explore ER-stress-related therapy are important on the treatment of neurodegenerative disease.
Li H.-T.,Nanjing Southeast University |
Li H.-T.,Baotou Medical College |
Wu X.-D.,Nanjing Southeast University |
Davey A.K.,University of South Australia |
Wang J.,University of South Australia
Phytotherapy Research | Year: 2011
The aim of the study was to investigate the effects of baicalin on blood glucose, insulin and cytokine levels. Rat diabetes was induced by intraperitoneal (i.p.) injection of nicotinamide and streptozotocin. Diabetic rats were dosed with i.p. baicalin or oral metformin daily for 8 days. Blood glucose, insulin and hepatic glycogen were determined using conventional methods. The activity of hepatic hexokinase was determined using a coupled assay with glucose-6-phosphate dehydrogenase. Serum levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and adiponectin were measured by enzyme-linked immunosorbent assay. Administration of baicalin at 50 or 100 mg/kg significantly decreased plasma glucose levels in a dose dependent manner. The serum insulin level was not increased by baicalin treatment. Administration of baicalin at a high dose (100 mg/kg) resulted in a significant increase of liver glycogen content and a reduction of serum TNF-α. The activity of hepatic hexokinase was significantly increased after dosing baicalin at 25, 50 or 10 mg/kg. Administration of baicalin (50 or 10 mg/kg) or metformin (10 mg/kg) significantly alleviated the morphological injury to the pancreas caused by STZ. The possible mechanisms contributing to the hypoglycemic effect include increasing the hepatic glycogen content and glycolysis, and reducing the serum levels of TNF-α. © 2010 John Wiley & Sons, Ltd.
Li F.,Shenyang University |
Li F.,Baotou Medical College |
Xuan J.,Shenyang University |
Xing J.,Shenyang University |
And 3 more authors.
Forensic Science International: Genetics | Year: 2014
Several commercial multiplex PCR kits for the amplification of short tandem repeat (STR) loci have been extensively applied in forensic genetics. Consequently, large numbers of samples have been genotyped, and the number of discordant genotypes observed has also increased. We observed allele dropout with two novel alleles at the STR loci TH01 and D13S317 during paternity testing using the AmpFℓSTR® Identifiler® PCR Amplification Kit. The lost alleles reappeared when alternative PCR primer pairs were used. A sequence analysis revealed a G-to-A substitution 82 bases downstream of the last TCAT motif of the repeat region at the TH01 locus (GenBank accession: D00269) and a G-to-T substitution 90 bases upstream of the first TATC motif of the repeat region at the D13S317 locus (GenBank accession: G09017). The frequencies of these two point mutations were subsequently investigated in the Chinese population using sequence-specific primer PCR (SSP-PCR), but neither of these mutations was detected in any of the samples tested. In addition, the DNA samples in which the mutations were identified were amplified to type the point mutations by SSP-PCR to determine the corresponding STR alleles at the two loci. Subsequently, the amplified PCR products with different point mutations and STR repeat numbers were directly sequenced because this strategy overcomes the appearance overlapping peaks generated by different STR alleles and accurately characterizes genotypes. Thus, our findings not only provide useful information for DNA databases and forensic identification but also establish an effective strategy for typing STR alleles with primer binding site mutations. © 2013 Elsevier B.V.
Ma B.,Nanjing Agricultural University |
Ma B.,Baotou Medical College |
Gao L.,Nanjing Agricultural University |
Zhang H.,Henan University of Science and Technology |
And 2 more authors.
Plant Cell Reports | Year: 2012
The effects of aluminum (Al) on root elongation, lipid peroxidation, hydrogen peroxide (H2O2) accumulation, antioxidant levels, antioxidant enzymatic activity, and lignin content in the roots of the Al-tolerant rice variety azucena and the Al-sensitive variety IR64 were investigated. Treatment with Al induced a greater decrease in root elongation and a greater increase in H2O2 and lipid peroxidation as determined by the total thiobarbituric acid-reactive substance (TBARS) level in IR64 than in azucena. Azucena had significantly higher levels of superoxide dismutase, ascorbate peroxidase, glutathione reductase, and glutathione peroxidase GSH POD activity compared with IR64. The concentrations of reduced glutathione (GSH) and ascorbic acid, and the GSH/GSSG ratio (reduced vs. oxidized glutathione) were also higher in azucena than in IR64 in the presence of Al. The addition of 1 mg/L GSH improved root elongation in both varieties and decreased H2O2 production under Al stress. By contrast, treatment with buthionine sulfoximine, a specific inhibitor of GSH synthesis, decreased root elongation in azucena and stimulated H2O2 production in both varieties. Moreover, Al treatment significantly increased the cytoplasmic activity of peroxidase (POD) as well as the levels of POD bound ionically and covalently to cell walls in the Al-sensitive variety. The lignin content was also increased. Treatment with exogenous H2O2 also increased the lignin content and decreased root elongation in IR64. These results suggest that Al induces lignification in the roots of Al-sensitive rice varieties, probably through an increase in H2O2 accumulation. © 2011 Springer-Verlag.
Yufu W.,Baotou Medical College
RISTI - Revista Iberica de Sistemas e Tecnologias de Informacao | Year: 2015
Objective: In order to improve the performance of the wrestling athletes in our country. Method: Establishing a research method of the wrestling winning laws based on logical reasoning and data statistics. Process: The article introduced the development of wrestling and related concepts, established the fuzzy logic reasoning model, and analyzed the technical and tactical characteristics of the classical style wrestling. Result &Analysis: This paper studies the various skills in wrestling, carries on statistics and analysis of a large number of wrestling sports records data, and finds that the establishment of the research method can get the general rule of wrestling. Result: the research method based on logical reasoning and data statistics is effective and feasible.
Yang R.-F.,Baotou Medical College |
Du H.-Y.,Inn Mongolia Peoples Hospital
International Eye Science | Year: 2016
Retinal vein occlusion is the second most common retinal vascular disease after diabetic retinopathy. The etiology and pathogenesis is still unclear. Macular edema and retinal ischemia is the main cause of vision loss. The article illustrates the advancement of retinal vein occlusion treated with medicine, laser and surgical treatment and mentions the prospects in the future. Copyright 2016 by the IJO Press.
Wang X.,Baotou Medical College |
Bai L.,Baotou Medical College |
Ma Y.,Baotou Medical College |
Ren Y.,Baotou Medical College |
Cong M.,Baotou Medical College
Cancer Research and Clinic | Year: 2015
C-myb is a member of the myb transcription factor family and involved in cell proliferation and differentiation. It activates specific target genes to regulate specific program through certain channel, and it takes signal transduction to regulate function and expression of protein. In this paper, c-myb, by binding to Wnt signaling pathway, makes Lgr5 start to promote cell proliferation and differentiation, and it can serve as a new therapeutic target to provide the basis of early diagnosis and treatment for colorectal cancer.