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Cheng L.,Tianjin Huanhu Hospital | Ma L.,Tianjin Huanhu Hospital | Ren H.,Tianjin Huanhu Hospital | Zhao H.,Jixian Peoples Hospital | And 3 more authors.
Molecular Medicine Reports | Year: 2014

Gamma knife surgery (GKS) is used for the treatment of various brain diseases. However, the mechanisms underlying brain injury following irradiation remain to be elucidated. Given that vascular endothelial growth factor (VEGF) is closely associated with pathological angiogenesis and the permeability of the blood brain barrier (BBB), the present study was designed to analyze temporal alterations in VEGF expression in the cerebral cortex and the effect of VEGF on cerebral edema in rats following GKS. Adult male Wistar rats were subjected to GKS at maximum doses of 60 Gy. Animals were sacrificed between 4 and 24 weeks after GKS. Immunohistochemistry, enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction (RT PCR) were employed for detecting VEGF expression. The vessel density was measured by CD31+ cell count and vascular structures were examined using electron microscopy. Brain water content and BBB permeability were measured in the present study. VEGF expression in the irradiated cortex progressively increased until 16 weeks after GKS when the maximal expression was reached, and then gradually decreased to the control level 24 weeks after GKS. These findings were confirmed by RT PCR. A mild decrease in vessel density was observed 4 weeks after GKS, followed by an increase in vessel density between 8 and 20 weeks later. Furthermore, previous studies also demonstrated vascular damage, opening of the BBB and an increase in brain water content occurring simultaneously. To the best of our knowledge, these data demonstrated for the first time dynamic changes in VEGF expression following GKS and also suggest the importance of VEGF expression in pathological angiogenesis and edema formation following GKS.


PubMed | Jixian Peoples Hospital, Fourth Hospital of Baotou, Tianjin Medical University, Tianjin Huanhu Hospital and First Hospital of Qinhuangdao
Type: Journal Article | Journal: Molecular medicine reports | Year: 2014

Gamma knife surgery (GKS) is used for the treatment of various brain diseases. However, the mechanisms underlying brain injury following irradiation remain to be elucidated. Given that vascular endothelial growth factor (VEGF) is closely associated with pathological angiogenesis and the permeability of the blood brain barrier (BBB), the present study was designed to analyze temporal alterations in VEGF expression in the cerebral cortex and the effect of VEGF on cerebral edema in rats following GKS. Adult male Wistar rats were subjected to GKS at maximum doses of 60Gy. Animals were sacrificed between 4 and 24weeks after GKS. Immunohistochemistry, enzymelinked immunosorbent assay and reverse transcriptionpolymerase chain reaction (RTPCR) were employed for detecting VEGF expression. The vessel density was measured by CD31+ cell count and vascular structures were examined using electron microscopy. Brain water content and BBB permeability were measured in the present study. VEGF expression in the irradiated cortex progressively increased until 16weeks after GKS when the maximal expression was reached, and then gradually decreased to the control level 24weeks after GKS. These findings were confirmed by RTPCR. A mild decrease in vessel density was observed 4weeks after GKS, followed by an increase in vessel density between 8 and 20weeks later. Furthermore, previous studies also demonstrated vascular damage, opening of the BBB and an increase in brain water content occurring simultaneously. To the best of our knowledge, these data demonstrated for the first time dynamic changes in VEGF expression following GKS and also suggest the importance of VEGF expression in pathological angiogenesis and edema formation following GKS.

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