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Baotou, China

Li K.,Chongqing Medical University | Li X.,Inner Mongolia University | Wu Z.,Chongqing Medical University | Zheng L.,Baotou Cancer Hospital | And 4 more authors.
Molecular Medicine Reports | Year: 2016

X-linked inhibitor of apoptosis (XIAP)-associated factor 1 (XAF-1), a tumor suppressor, is downregulated in most human malignant tumors. However, the tumor suppressive role of XAF-1 in hepatocellular carcinoma (HCC) and its therapeutic value require further elucidation. The present study examined the expression of XAF-1 at the mRNA and protein level in the HCC and paired peritumor tissue specimens, as well as in HCC cell lines and a normal liver cell line. A recombinant adenovirus which co-expressed XAF-1 and TNF-α was then constructed, and its effects on the proliferation and colony formation ability of the MHCC97H HCC cell line were assessed using apoptosis induction, flow cytometry, trypan blue staining assay and a clonogenic assay. The results demonstrated that the expression of XAF-1 was significantly reduced in HCC tissues compared with that in their matched peritumor specimens, and a significant correlation with the tumor size, stage and tumor -nodes -metastasis stage was identified. The reduced levels of XAF-1 were further confirmed the HCC cell lines MHCC97L, HepG2 and MHCC97H compared with those in the L02 normal liver cell line. The recombinant adenovirus Ad-XAF-1andTNF-α, which co-expressed XAF-1 and TNF-α, was shown to efficiently express the two proteins at the mRNA and protein level. Furthermore, infection with Ad-XAF-1andTNF-α synergistically induced apoptosis, reduced the proliferation and colony formation ability of MHCC97L cells to a significantly greater extent than overexpression of XAF-1 or TNF-α individually. To the best of our knowledge, the present study was the first to construct an adenovirus which co-expressed XAF-1 and TNF-α in the same open reading frame and expressed them proportionally. As Ad-XAF-1andTNF-α inhibited HCC cells with enhanced efficiency, it may be applicable for the treatment of HCC. Source


Li X.,Baotou Cancer Hospital | Zhao X.-X.,Baotou Cancer Hospital | Xu F.,Baotou Cancer Hospital
Journal of Practical Oncology | Year: 2011

Objective: To evaluate the safety and efficacy of transurethral plasma cutting operation (TURis-Bt) in treatment of bladder carcinoma. Methods: The clinical data of 101 cases of bladder carcinoma invading muscular layer were retrospectively analyzed. Fifty-four patients underwent transurethral plasma cutting operation, among whom 50 were followed-up for 24-60 months. Forty-seven patients were treated with partial bladder resection and followedup for 1-60 months. Both groups received bladder instiUation of pirarubicin (THP). Results: The recurrence rate of TURis-Bt group was 29.6% (16/54), while that of partial bladder resection group was 29.8% (14/47). There was no significant difference between two groups. Conclusion: TURis-Bt can be used to treat bladder carcinoma invading muscular layer, especially for high-risk patients or patients who are unwilling to accept open surgery. Source


Wang H.-M.,Baotou Cancer Hospital | Zhou S.,Baotou Cancer Hospital | Wang W.,Baotou Cancer Hospital
World Chinese Journal of Digestology | Year: 2014

Aim: To analyze the incidence trends and characteristics of colorectal cancer in Inner Mongolia urban and rural areas.Methods: According to colorectal cancer incidence database derived from Inner Mongolia tumor registries from 2009 to 2013, there were a total population of 24706321 (including both urban and rural areas). ICD-9 and ICD-0-3 were used in the registries. Incidence trends were analyzed using the percentage change and the annual percent change (APC).Results: From 2009 to 2013, colorectal cancer incidence was 29.18/105 in Inner Mongolia, with 33.42/105 and 14.55/105 observed in urban and rural areas, respectively. The adjusted incidence rate in the Chinese standards was 22.98/105. From 2009 to 2013, colorectal cancer incidence increased by 18.93% (18.91% in urban areas and 10.84% in rural areas).Conclusion: Colorectal cancer incidence rate in rural areas in China is lower than that in urban areas. An increasing incidence rate was observed in rural areas in contrast to the decreasing trend in urban areas. © 2014 Baishideng Publishing Group Inc. All rights reserved. Source


Li K.,Chongqing Medical University | Guo X.,Baotou Cancer Hospital | Wang Z.,Chongqing Medical University | Li X.,Baotou Cancer Hospital | And 4 more authors.
OncoTargets and Therapy | Year: 2016

Increased aldehyde dehydrogenase 1 (ALDH1) activity has been determined to be present in the stem cells of several kinds of cancers including gastric cancer (GC). Nevertheless, which ones of ALDH1’s isoenzymes are leading to ALDH1 activity remains elusive. In this study, we examined the prognostic value and hazard ratio (HR) of individual ALDH1 isoenzymes in patients with GC using “The Kaplan-Meier plotter” database. mRNA high expression level of ALDH1A1 was not found to be significantly correlated with the overall survival (OS) of all patients with GC followed for 20 years, HR =0.86 (95% confidence interval [CI]: 0.7-1.05), P=0.13. mRNA high expression level of ALDH1A2 was also not significantly correlated with OS for all patients with GC, HR =1.13 (95% CI: 0.91-1.41), P=0.25. mRNA high expression level of ALDH1A3 was found to be significantly correlated with worsened OS in either intestinal-type patients, HR =2.24 (95% CI: 1.44-3.49), P=0.00026, or diffuse-type patients, HR =1.91 (95% CI: 1.02-3.59), P=0.04. Interestingly, mRNA high expression level of ALDH1B1 was found to be significantly correlated with better OS for all patients with GC, HR =0.66 (95% CI: 0.53-0.81), P=7.8e-05, and mRNA high expression level of ALDH1L1 was found to be significantly correlated with worsened OS for all patients with GC, HR =1.23 (95% CI: 1-1.51), P=0.048. Furthermore, our results also indicate that ALDH1A3 and ALDH1L1 are potential major contributors to the ALDH1 activity in GC, since mRNA high expression levels of ALDH1A3 and ALDH1L1 were found to be significantly correlated with worsened OS for all patients with GC. Based on our study, ALDH1A3 and ALDH1L1 are potential prognostic markers and therapeutic targets for patients with GC. © 2016 Li et al. Source


Zhang Q.,Baotou Cancer Hospital | Shi B.,Baotou Cancer Hospital | Liu Z.,Baotou Cancer Hospital | Zhang M.,Baotou Cancer Hospital | Zhang W.,Beijing Institute of Technology
OncoTargets and Therapy | Year: 2013

Background: This study used CT (computed tomography) and magnetic resonance imaging (MRI) to identify correlations between perfusion parameters for squamous cell lung carcinoma and tumor angiogenesis in a rabbit model of VX2 lung cancer. Methods: VX2 tumors were implanted in the lungs of 35 New Zealand White rabbits. CT and MRI perfusion scanning were performed on days 14, 17, 21, 25, and 28 after tumor implantation. CT perfusion parameters were perfusion, peak enhanced increment, transit time peak, and blood volume, and MRI perfusion parameters were wash in rate, wash out rate, maximum enhancement rate, and transit time peak. CT and MRI perfusion parameters were obtained at the tumor rim, in the tumor tissue, and in the muscle tissue surrounding the tumor. Results: On CT perfusion imaging, t values for perfusion, peak enhanced increment, and blood volume (tumor rim versus muscle) were 16.31, 11.79, and 5.21, respectively (P, 0.01); t values for perfusion, peak enhanced increment, and blood volume (tumor versus muscle) were 9.87, 4.09, and 5.35, respectively (P, 0.01); and t values for transit time peak were 1.52 (tumor rim versus muscle) and 1.29 (tumor versus muscle), respectively (P. 0.05). On MRI perfusion imaging, t values for wash in rate, wash out rate, and maximum enhancement rate (tumor rim versus muscle) were 18.14, 8.79, and 6.02, respectively (P, 0.01); t values for muscle wash in rate, wash out rate, and maximum enhancement rate (tumor versus muscle) were 9.45, 8.23, and 4.21, respectively (P, 0.01); and t values for transit time peak were 1.21 (tumor rim versus muscle) and 1.05 (tumor versus muscle), respectively (P. 0.05). Conclusion: A combination of CT and MRI perfusion imaging demonstrated hemodynamic changes in a rabbit model of VX2 lung cancer, and provides a theoretical foundation for treatment of human squamous cell lung carcinoma. © 2013 Zhang et al, publisher and licensee Dove Medical Press Ltd. Source

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