Baoshan Sugarcane Research Institute

Longyang District, China

Baoshan Sugarcane Research Institute

Longyang District, China
SEARCH FILTERS
Time filter
Source Type

Li W.-F.,Yunnan Academy of Agricultural Sciences | Wang X.-Y.,Yunnan Academy of Agricultural Sciences | Huang Y.-K.,Yunnan Academy of Agricultural Sciences | Shen K.,Yunnan Academy of Agricultural Sciences | And 6 more authors.
Canadian Journal of Plant Pathology | Year: 2013

The introduction of the sugarcane white leaf (SCWL) phytoplasma into Yunnan Province, China was confirmed for the first time by nested PCR. Sequencing and analysis of 17 nested PCR products of the 16S rDNA of the phytoplasma from random samples showed that these sequences were identical. A BLAST search indicated that the sequences were highly homologous (99.05-100% similarity) to the corresponding genome region in GenBank. Field surveys showed that disease incidence among affected cultivars was 13-52% and up to 100% in seriously infected fields. The sugarcane cultivar 'Yuetang 86-368' was highly susceptible. SCWL is a destructive disease on sugarcane that requires preventive quarantine management measures to prevent its further spread. © 2013 The Canadian Phytopathological Society.


Wang X.-Y.,Yunnan Academy of Agricultural Sciences | Li W.-F.,Yunnan Academy of Agricultural Sciences | Huang Y.-K.,Yunnan Academy of Agricultural Sciences | Zhang R.-Y.,Yunnan Academy of Agricultural Sciences | And 6 more authors.
Tropical Plant Pathology | Year: 2014

Sugarcane white leaf (SCWL) is an important disease caused by a phytoplasma. In Baoshan, Yunnan, China, SCWL was firstly observed in 2012, and has extended its area of occurrence to 600 hm2. Up to 52% of the plants may become diseased in a field and even complete loss of cane yield may result in the heavily infected fields, posing a serious threat to Yunnan sugar industry. To ascertain the causal agent of suspected SCWL disease in Yunnan, nested PCR using two sets of phytoplasma primer pairs (MOLX/MLOY and P1/ P2) was used to successfully amplify a genomic region of the 16S ribosomal DNA (16S rDNA) from 36 suspected SCWL samples. On the basis of sequencing, phylogenetic analysis and nucleotide alignments of 17 nested PCR products randomly selected from positive samples, identical fragments of 210bp in length were obtained that could be clustered into the 6Sr group XI (Rice Yellow Dwarf group) and shared 100% identity with the 16S-23S intergenic spacer region (ISR) of a member of this group, the SCWL phytoplasma (GenBank: HQ917068), and 99.52% with Sugarcane grassy shoot phytoplasma, of the same group. These results indicate that the SCWL disease in Baoshan is caused by a phytoplasma of the 6Sr group XI.

Loading Baoshan Sugarcane Research Institute collaborators
Loading Baoshan Sugarcane Research Institute collaborators