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Yang J.-H.,Baoji Municipal Central Hospital
Journal of Interventional Radiology (China) | Year: 2013

Objective: To discuss the hemostasis effect of rapid manual compression performed immediately after the catheterization through femoral artery, and to analyze its complications. Methods: Based on the author's experience obtained from dozens of years' practice in interventional field, the author summarized an effective hemostasis technique, which was performed through rapid manual compression on the puncturing point. The mean compression time with hand was only about two minutes. This manipulation procedure for hemostasis had been carried out in 324 patients immediately after the catheterization through femoral artery was finished. One day after the catheterization, observation of the skin around the puncture point and palpation of the puncturing site were conducted. Results: Rapid manual compression hemostasis was successfully carried out in all 324 patients, of whom subcutaneous ecchymosis around the puncture site was seen in only four. The effective hemostasis rate was 98.8% (320/324). In another six patients mild skin injury due to compression around the puncture site was observed, which, without any medication, recovered spontaneously in one week. Conclusion: For the hemostasis of femoral puncturing point after catheterization, manual compression method employed immediately after the interventional procedure is finished is a time - saving, labour - saving, safe, economical, repeatable, environment - protecting and practical hemostatic technique. Source

Che H.,PLA Fourth Military Medical University | Che H.,Xian Jiaotong University | Song J.,Baoji Municipal Central Hospital | Guo S.,Xian Jiaotong University | And 2 more authors.
Oncology Reports | Year: 2013

Angiogenesis is crucial for the development and metastasis of human brain glioma. Based on our previous successful construction of a lentivirus-mediated alphastatin (an endogenous angiogenesis inhibitor) gene transfer system and our findings that alphastatin exhibited potent inhibitory effects on the migration and differentiation of human umbilical vein endothelial cell lines (HUVECs) induced by vascular endothelial growth factor (VEGF) or basic fibroblast growth factor (bFGF) in vitro, here, we investigated the effect of using lentiviral vectors to overexpress alphastatin in human glioma cells to show whether sustained long-term expression of alphastatin diminishes tumor growth in a xenograft glioma model. We found that the transduced glioma cells sustainedly secreted alphastatin, which did not affect the proliferative ability of the glioma cells. Furthermore, tumor xenografts treated with the recombinant lentivirus were significantly smaller compared to the control xenografts and vascularity within the treated tumors was evidently decreased. Our data suggest that stable expression of alphastatin inhibits human glioma growth by inhibiting angiogenesis, with a probable mechanism of suppressing the turnover of VE-cadherin membrane molecules. Source

Zhao Y.-N.,Baoji Municipal Central Hospital | Guo X.,University of Sichuan | Ma Z.-G.,University of Sichuan | Gu L.,University of Sichuan | And 2 more authors.
Medical Oncology | Year: 2011

Although glucocorticoids (GCs) have been used to treat acute lymphoblast leukemia (ALL) for decades, the mechanisms of GC sensitivity and resistance in ALL cells are poorly understood. This study investigated the role and mechanisms of pro-apoptotic protein BIM in apoptosis of GC-sensitive and- resistant ALL cells. The dramatic apoptosis was observed in GC-sensitive CEM-C7 cells after incubated with DEX for 48 h, while not in GC-resistant CEM-C1 cells. The significant up-regulation of BIM in CEM-C7 cells induced by DEX was also observed, but no up-regulation of BIM was detected in DEX-induced CEM-C1 cells. When treated with DEX plus RU486, a glucocorticoid receptor blocker, the apoptosis and BIM expression of CEM-C7 cells were canceled. P38MAPK-blocking pharmacon SB203580 also significantly inhibited the up-regulation of BIM in CEM-C7 cells. These suggested that the absence of BIM up-regulation is one of the important mechanisms of GC resistance, GC-GR conjugation is indispensible in both GC-induced apoptosis and up-regulation of BIM, and p38 MAPK signal pathway is also involved in this process. © 2010 Springer Science+Business Media, LLC. Source

Xiong H.-Y.,PLA Fourth Military Medical University | Liu Y.,PLA Fourth Military Medical University | Shu D.-C.,Baoji Municipal Central Hospital | Zhang S.-L.,Shaanxi General Hospital of CAPF | And 5 more authors.
ASAIO Journal | Year: 2016

The effects of sevoflurane inhalation during cardiopulmonary bypass (CPB) on postoperative courses and serum cardiac troponin I (cTnI) concentrations in pediatric patients undergoing cardiac surgery have not been extensively investigated. In this single-center, prospective, randomized trial, an anesthetic regimen containing 2% sevoflurane used throughout the CPB process was compared with a total intravenous anesthesia (TIVA) regimen. One hundred and three patients undergoing congenital heart defect repair with CPB were included in this prospective randomized controlled study. They were randomized into two groups: The sevoflurane group, who received 2% sevoflurane during CPB via an oxygenator, and the control group, who received only an oxygen-air mixture. The pre-and intra-operative parameters were comparable between the two groups. There was a slight but significant increase of arterial diastolic pressure in the sevoflurane group immediately after CPB compared with control patients (46.9 ± 9.3 mm Hg vs. 43.6 ± 8.9 mm Hg; p = 0.033). There was no death in either group. The postoperative ventilation time (in mean [95% confidence interval]) was shorter in the sevoflurane group than that in the control group (26.1 [19.2, 33.0] h vs. 37.7 [24.4, 50.9] h; p = 0.014). The postoperative ICU time, hospital days, and serial serum cTnI concentrations were not significantly different between the two groups. Inhalation of 2% sevoflurane during CPB is beneficial to the recovery of pediatric patients undergoing cardiac surgery but has no significant effect on postoperative cTnI release. © Copyright 2015 by the American Society for Artificial Internal Organs. Source

Wu D.D.,Baoji Municipal Central Hospital | Xiao Y.F.,Xian Jiaotong University | Geng Y.,Baoji Municipal Central Hospital | Hou J.,Baoji Municipal Central Hospital
Cancer Genetics and Cytogenetics | Year: 2010

We sought to investigate the efficacy of arsenic trioxide (As2O3) against a human gastric cell line implanted in nude mice in vivo, as well as the mechanism involved. The solid tumor model was created in nude mice with the gastric cancer cell line SGC-7901. The animals were randomly divided into three groups. As2O3 was injected into animals in two arsenic-treated groups (2.5 mg/kg and 5 mg/kg), and the same volume of saline solution was injected into the control group. The inhibitory effect was observed in every group. Apoptotic cells and apoptotic bodies were observed by transmission electron microscope; the fraction of apoptotic cells was detected by TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling) under laser confocal technology. The expression of Fas and FasL was detected by immunohistochemical staining. In nude mice, after treatment with 5 mg/kg and 2.5 mg/kg As2O3, approximately 50% and 30% tumor growth inhibition were observed, respectively (P < 0.05 for both treatment groups). Increase in apoptotic cells and apoptotic bodies appeared in As2O3-treated tumors compared with the control group. The fluorescence intensity levels of apoptotic cells in tumor were significantly higher in the arsenic-treated groups (P < 0.05 for both treatment groups). The fluorescence intensity level of apoptotic cells in the 5-mg/kg group was higher than that in the 2.5-mg/kg group (P < 0.05). The expression of Fas protein increased in dose- and time-dependent manner after the treatment with As2O3, but that of FasL protein showed no significant difference between control and treated groups. As2O3 did not induce hepatic and renal system injury in the nude mice. As2O3 can inhibit the growth of human gastric cell implanted tumor. We ascribe this to upregulation of Fas, which can induce apoptosis of gastric cells. © 2010 Elsevier Inc. Source

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