Baoji Central Hospital
Baoji Central Hospital
Liu Y.,Tongchuan Kuangwuju Central Hospital |
Chen X.,Baoji Central Hospital |
Li J.,Sun Yat Sen University
Molecular Medicine Reports | Year: 2017
Resveratrol, a natural phytochemical found in grapes and red wine, has been found to possess protective effects against endothelial cell apoptosis and oxidative damage. Oxidized-low density lipoprotein (ox-LDL) can induce apoptosis of endothelial cells, which is an important initial event in several cardiovascular diseases. However, the effect of resveratrol on ox-LDL-induced apoptosis and oxidative damage, and the possible associated mechanisms remain to be elucidated. In the present study, following exposure to ox-LDL, human umbilical vein endothelial cells (HUVECs) were treated with or without resveratrol. Cell viability was examined using Cell Counting Kit-8 and 5-bromo-2'-deoxyuridine uptake assays, respectively. Cell apoptosis was determined by flow cytometry. Apoptosis-associated markers were detected using western blot analysis. Oxidative stress was analyzed using molecular and biochemical approaches. Resveratrol restored ox-LDL-induced HUVEC injury and apoptosis in a dose-dependent manner. In addition, compared with the control group, ox-LDL treatment decreased the B cell lymphoma-2 (Bcl-2)/Bcl-2-associated X protein ratio, mitochondrial membrane potential and activation of superoxide dismutase, and enhanced the release of mitochondrial cytochrome c into the cytoplasm, the activation of caspase and lipid peroxidation. All these alterations were signifcantly inhibited following treatment with resveratrol. The results demonstrated that resveratrol prevented HUVEC apoptosis through inhibiting mitochondria-derived oxidative damage. These fndings may provide a novel mechanism by which resveratrol prevents against endothelial cell apoptosis.
Li R.,The Peoples Hospital Of Baoji City |
Chen J.-R.,Baoji Central Hospital
Therapeutics and Clinical Risk Management | Year: 2016
Purpose: The purpose of this study is to evaluate the therapeutic efficacy and safety of stem cells for the treatment of patients with ST-segment elevation myocardial infarction (STEMI). Materials and methods: We performed a systematic review and meta-analysis of relevant published clinical studies. A computerized search was conducted for randomized controlled trials of stem cell therapy for STEMI. Results: Twenty-eight randomized controlled trials with a total of 1,938 STEMI patients were included in the present meta-analysis. Stem cell therapy resulted in an improvement in long-term (12 months) left ventricular ejection fraction of 3.15% (95% confidence interval 1.01–5.29, P<0.01). The 3-month to 4-month, 6-month, and 12-month left ventricular end-systolic volume showed favorable results in the stem cell therapy group compared with the control group (P≤ 0.05). Significant decrease was also observed in left ventricular end-diastolic volume after 3-month to 4-month and 12-month follow-up compared with controls (P<0.05). Wall mean score index was reduced significantly in stem cell therapy group when compared with the control group at 6-month and 12-month follow-up (P=0.01). Moreover, our analysis showed a significant change of 12-month infarct size decrease in STEMI patients treated with stem cells compared with controls (P<0.01). In addition, no significant difference was found between treatment group and control in adverse reactions (P>0.05). Conclusion: Overall, stem cell therapy is efficacious in the treatment of patients with STEMI, with low rates of adverse events compared with control group patients. © 2016 Li et al.
Gao Z.,Baoji Central Hospital |
Zhen S.,Chinese Medicine Hospital of Baoji
Cancer Research and Clinic | Year: 2017
Objective: To evaluate the changes of serum total prostate specific antigen (TPSA) with age and its significance in differential diagnosis of prostate cancer (PCa) and prostatic hyperplasia (BPH). Methods: TPSA and free prostate specific antigen (FPSA) in 146 cases of PCa, 108 cases of BPH and 210 cases of healthy subjects were detected by electrochemiluminescence. Results There was a positive correlation between age and serum TPSA level (r = 0.265, P < 0.01). The levels of TPSA in PCa and BPH groups were (85.40 ±56.70) μg/L and (7.90 ±7.00) μg/L, and the difference was statistically significant (f = 8.310, P= 0.001); FPSA levels were (5.16±4.90) μg/L, (1.50±1.36) μg/L, and the difference was statistically significant (i = 3.152, P = 0.030). In patients with TPSA levels ranging from 4.0 to 20.0 μg/L ("diagnostic gray zone"), the levels of TPSA in PCa and BPH groups were (8.82±4.01) μg/L and (8.41±3.95) μg/L, and the difference had no statistical significance (f = 0.198, P = 0.256); The levels of FPSA were (1.18 ±0.91) μg/L and (2.32±1.20) μg/L, the ratio of FPSA/TPSA were 0.12±0.08 and 0.24±0.23, and the differences were statistically significant (f = 23.56, P = 0.020; t = 32.45, P = 0.006). When FPSA/TPSA ratio was 0.16, its sensitivity and specificity for PCa were 84.4 % and 79.8 %, respectively. Conclusions Serum TPSA levels vary with age, and 95 % of all age groups should be used as the medical criterion to improve the specificity and accuracy of PSA in diagnosing with PCa. The ratio of FPSA/TPSA has a significant clinical value in differential diagnosis of PCa and BPH in patients with TPSA in "diagnostic gray zone".
Song N.,Baoji Central Hospital |
Sun C.,Liaocheng Traditional Chinese Hospital |
Wang Y.,Ankang Hospital Traditional Chinese Medicine
International Journal of Clinical and Experimental Pathology | Year: 2017
Wnt/β-catenin pathway regulates cell proliferation and apoptosis via facilitating Survivin transcription. Over-expression of β-catenin is correlated with osteosarcoma onset and drug resistance. MiR-340 is down-regulated in osteosarcoma, and has targeted relationship with 3'-UTR of β-catenin. This study investigated the role of miR-340 in regulating β-catenin expression, and affecting osteosarcoma Saos-2 cells drug resistance to Adriamycin (ADM). MiR-340 and β-catenin expression was compared between Saos-3 and hFOB1.19 cells. Dual luciferase gene reporter assay demonstrated regulatory relationship between miR-340 and β-catenin. ADP-resistant cell line Saos-2/ ADM1.0 and Saos-2/ADM4.0 was generated for quantifying miR-340, β-catenin and Survivin expression. Drug resistant index was calculated and compared. 4.0 mg/L ADM was used to treat Saos-2/ADM4.0 cells, which were then treated with miR-340 mimic and/or si-β-catenin. Cell expression of β-catenin and Survivin, and cell proliferation/ apoptosis were compared. Saos-2 cells had lower miR-340 and higher β-catenin expression compared to hFOB1.19 cells. A targeted regulation relationship existed between miR-340 and β-catenin. MiR-340 expression in Saos-2/ ADM4.0 (RI=44.528) and Saos-2/ADM1.0 (RI=21.937) cells was lower than Saos-2 cells, whilst β-catenin and Survivin expression was higher. Transfection of miR-340 mimic and/or si-β-catenin lowered β-catenin and Survivin expression, weakened cell proliferation potency, and increased ADM-induced apoptosis. MiR-340 can decrease Saos-2 cell proliferation, elevate apoptosis and ADM sensitivity via targeted inhibition on β-catenin and downstream anti-apoptotic protein Survivin expression.
Jia S.,PLA Fourth Military Medical University |
Liu L.,Baoji Central Hospital |
Pan W.,Xi'an Physical Education University |
Meng G.,PLA Fourth Military Medical University |
And 4 more authors.
Journal of Bioscience and Bioengineering | Year: 2012
The structure of a cartilage scaffold is required to mimic native articular cartilage, which has an oriented structure associated with its mechanical function. In this study, an oriented cartilage extracellular matrix (ECM)-derived scaffold was fabricated composed of microtubules arranged in parallel in vertical section. The mechanical property was higher than that of a typical non-oriented scaffold (. p<. 0.05). Oriented and non-oriented scaffolds were seeded with chondrogenic-induced bone mesenchymal stem cells and cell-scaffold constructs were implanted subcutaneously in the dorsa of nude mice. At 4. weeks, all samples stained positive for safranin O, toluidine blue, and collagen type II, but negative for collagen type I. Oriented-structure constructs contained numerous parallel giant bundles of densely packed collagen fibers with chondrocyte-like cells aligned along the fibers. Total DNA, glycosaminoglycans and collagen contents increased with time and these values were similar in the two groups. Compared with the native articular cartilage, the Young's modulus of the tissue-engineered (TE) cartilage reached 42.9%, 23.0% in oriented and non-oriented scaffolds respectively, at 4. weeks. These results indicate that oriented ECM-derived scaffolds enhance the biomechanical property of TE cartilage and thus represent a promising approach to cartilage tissue engineering. © 2011 The Society for Biotechnology, Japan.
Chen L.,George Washington University |
Li Y.,Baoji Central Hospital |
Fu Y.,George Washington University |
Peng J.,George Washington University |
And 9 more authors.
PLoS ONE | Year: 2013
MicroRNAs (miRNAs) contribute to cancer initiation and progression by silencing the expression of their target genes, causing either mRNA molecule degradation or translational inhibition. Intraductal epithelial proliferations of the breast are histologically and clinically classified into normal, atypical ductal hyperplasia (ADH), ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC). To better understand the progression of ductal breast cancer development, we attempt to identify deregulated miRNAs in this process using Formalin-Fixed, Paraffin-Embedded (FFPE) tissues from breast cancer patients. Following tissue microdissection, we obtained 8 normal, 4 ADH, 6 DCIS and 7 IDC samples, which were subject to RNA isolation and miRNA expression profiling analysis. We found that miR-21, miR-200b/c, miR-141, and miR-183 were consistently up-regulated in ADH, DCIS and IDC compared to normal, while miR-557 was uniquely down-regulated in DCIS. Interestingly, the most significant miRNA deregulations occurred during the transition from normal to ADH. However, the data did not reveal a step-wise miRNA alteration among discrete steps along tumor progression, which is in accordance with previous reports of mRNA profiling of different stages of breast cancer. Furthermore, the expression of MSH2 and SMAD7, two important molecules involving TGF-β pathway, was restored following miR-21 knockdown in both MCF-7 and Hs578T breast cancer cells. In this study, we have not only identified a number of potential candidate miRNAs for breast cancer, but also found that deregulation of miRNA expression during breast tumorigenesis might be an early event since it occurred significantly during normal to ADH transition. Consequently, we have demonstrated the feasibility of miRNA expression profiling analysis using archived FFPE tissues, typically with rich clinical information, as a means of miRNA biomarker discovery. © 2013 Chen et al.
Niu X.,Xi'an Jiaotong University |
Cao W.,Xi'an Jiaotong University |
Ma H.,Xi'an Jiaotong University |
Feng J.,Xi'an Jiaotong University |
And 2 more authors.
Journal of Dermatology | Year: 2012
T-helper (Th) cells, including Th1, Th2 and Th17 cells, may play an important role in the pathogenesis of psoriasis vulgaris (PV). Acitretin is an effective treatment for PV; however, its influence on Th cells during the treatment of PV is unclear. This study aimed to investigate the influence of acitretin on Th1, Th2 and Th17 cells in PV patients. PV patients (n = 30) received acitretin p.o. (20 mg/day) for 8 weeks. Sera and skin biopsies were obtained before and after treatment. Double-labeled immunofluorescence was used to analyze T, Th1, Th2 and Th17 cells in skin lesions. Enzyme-linked immunosorbent assay and western blot were used to analyze the expressions of interferon (IFN)-γ, interleukin (IL)-4 and IL-17 in sera and skin lesions. The expressions of IFN-γ mRNA, IL-4 mRNA and IL-17 mRNA in skin lesions were detected by in situ hybridization. Acitretin decreased the quantity of T, Th1 and Th17 cells in PV lesions, but had no significant influence on Th2 cells. Acitretin also decreased the expression of IFN-γ and IL-17 in serum and lesions. The expressions of IFN-γ mRNA and IL-17 mRNA decreased significantly after 8 weeks of therapy. However, acitretin had no significant influence on the expression of IL-4 protein and mRNA. Acitretin can reverse Th1 and Th17 preponderance in PV patients to some degree. This may be due to the mechanism of acitretin on PV; however, Th2 cells were not affected by acitretin treatment. © 2012 Japanese Dermatological Association.
Wu Z.-X.,PLA Fourth Military Medical University |
Gong F.-T.,Xian Hospital of Traditional Chinese Medicine |
Liu L.,Baoji Central Hospital |
Ma Z.-S.,PLA Fourth Military Medical University |
And 5 more authors.
Archives of Orthopaedic and Trauma Surgery | Year: 2012
Introduction The aim of this study is to compare the rate of screw loosening and clinical outcomes of expandable pedicle screws (EPS) with those of conventional pedicle screws (CPS) in patients treated for spinal stenosis (SS) combined with osteoporosis. Methods One hundred and fifty-seven consecutive patients with SS received either EPS fixation (n = 80) or CPS fixation (n = 77) to obtain lumbosacral stabilization. Patients were observed for a minimum of 24 months. Outcome measures included screw loosening, fusion rate, Japanese Orthopaedic Association (JOA) scores and Oswestry disability index (ODI) scoring system, and complications. Results In the EPS group, 20 screws became loose (4.1%) in 6 patients (7.5%), and two screws (0.4%) had broken. In the CPS group, 48 screws became loose (12.9%) in 15 patients (19.5%), but no screws were broken. The fusion rate in the EPS group (92.5%) was significantly higher than that of the CPS group (80.5%). The rate of screw loosening in the EPS group (4.1%) was significantly lower than that of the CPS group (12.9%). Six EPS (1.8%) screws were removed. In the EPS group, two screws had broken but without neural complications. Twelve months after surgeries, JOA and ODI scores in the EPS group were significantly improved. There were four cases of dural tears, which healed after corresponding treatment. Conclusions EPS can decrease the risk of screw loosening and achieve better fixation strength and clinical results in osteoporotic lumbar spine fusion. © 2011 Springer-Verlag.
Liu J.,PLA Fourth Military Medical University |
Yao Y.,Baoji Central Hospital |
Ding H.,Shengli Oilfield Central Hospital |
Chen R.,PLA Fourth Military Medical University
Tumor Biology | Year: 2014
With the objective of identifying promising antitumor agents for human leukemia, we carried out to determine the anticancer ability of oxymatrine on the human leukemia HL-60 cell line. In vitro experiments demonstrated that oxymatrine reduced the proliferation of HL-60 cells in a dose- and time-dependent manner via the induction of apoptosis and cell cycle arrest at G2/M and S phases. The proteins involved in oxymatrine-induced apoptosis in HL-60 cells were also examined using Western blot. The increase in apoptosis upon treatment with oxymatrine was correlated with downregulation of anti-apoptotic Bcl-2 expression and upregulation of pro-apoptotic Bax expression. Furthermore, oxymatrine induced the activation of caspase-3 and caspase-9 and the cleavage of poly(ADP-ribose) polymerase (PARP) in HL-60 cells. In addition, pretreatment with a specific caspase-3 (Z-DEVD-FMK) or caspase-9 (Z-LEHD-FMK) inhibitor significantly neutralized the pro-apoptotic activity of oxymatrine in HL-60 cells, demonstrating the important role of caspase-3 and caspase-9 in this process. Taken together, these results indicated that oxymatrine-induced apoptosis may occur through the activation of the caspase-9/caspase-3-mediated intrinsic pathway. Therefore, oxymatrine may be a potential candidate for the treatment of human leukemia. © 2014 International Society of Oncology and BioMarkers (ISOBM).
Wan J.,General Hospital of Lanzhou |
Xia L.,Baoji Central Hospital |
Liang W.,General Hospital of Lanzhou |
Liu Y.,General Hospital of Lanzhou |
Cai Q.,General Hospital of Lanzhou
Journal of Diabetes Research | Year: 2013
In this paper, we established a delayed wound healing model on diabetic rat to mimic the pathophysiology of clinical patients who suffered from diabetic foot ulcers. We also evaluated if transplantation of allogeneic bone marrow-derived mesenchymal stem cells could promote the delayed wound healing and investigated the possible underlying biological mechanisms and stem cell behavior involved in this process. The results showed that bone marrow-derived mesenchymal stem cells had a positive effect on delayed wound healing in diabetic rats. Intramuscular transplantation demonstrated the best efficacy. This effect is associated with granulation tissue formation, angiogenesis, cellular proliferation, and high vascular endothelial growth factor expression in wound tissues. In addition, bone marrow-derived mesenchymal stem cells have been shown to mobilize and find home for ischemic and wounded tissues to participate in the process of wound healing. Intramuscular transplantation of exogenous isogeneic stem cells may be suitable for clinical application in the treatment of diabetic foot ulcers although the safety of this therapy should be considered. © 2013 Jiangbo Wan et al.