Baoding First Central Hospital
Baoding First Central Hospital
Li Y.,Institute of Polymer Chemistry |
Liu Y.,Institute of Polymer Chemistry |
Ma R.,Institute of Polymer Chemistry |
Ma R.,Nankai University |
And 6 more authors.
ACS Applied Materials and Interfaces | Year: 2017
Stimuli-sensitive hydrogels are ideal candidates for biomedical and bioengineering purposes, although applications of hydrogels may be limited, due in part to the limited choice of suitable materials for constructing hydrogels, the complexity in the synthesis of the source materials, and the undesired fast-then-slow drug-release behaviors of usual hydrogels. Herein, we describe the fabrication of a new supramolecular guanosine (G)-quadruplex hydrogel by multicomponent self-assembly of endogenous guanosine (G), 2-formylboronic acid (2-FPBA), and tris(2-aminoethyl)amine (TAEA) in the presence of KCl in an easy and convenient way. The features of the G-quadruplex hydrogel include (1) versatility and commercial availability of building blocks with different functions, (2) dynamic iminoboronate bonds with pH and glucose responsiveness, and (3) zero-order drug-release behavior because of the superficial peel-off of the hydrogel in response to stimuli. The structure, morphology, and properties of the G-quadruplex hydrogel were well-characterized, and satisfactory zero-order drug release was successfully achieved. This kind of supramolecular G-quadruplex hydrogels may find applications in biological fields. © 2017 American Chemical Society.
Ma R.,Nankai University |
Zhang C.,Nankai University |
Liu Y.,Nankai University |
Li C.,Nankai University |
And 5 more authors.
RSC Advances | Year: 2017
Amphiphilic block copolymer micelles in aqueous solution have been used extensively in delivery of hydrophobic drugs with the hydrophobic core serving as the reservoir. However, encapsulation of hydrophilic drugs by polymeric micelles with hydrophobic core is relatively difficult because of the poor interaction between them. Herein, we report a novel kind of pH/GSH dual-responsive complex micelles with a hydrophilic drug loaded, via direct subcomponent self-assembly of block copolymer poly(ethyleneglycol)-block-poly(l-lysine) (PEG-b-PLys), 2-formylbenzeneboronic acid (2-FPBA) and hydrophilic 1,2-diol-containing drugs (e.g. capecitabine (CAPE)) under physiological pH 7.4 based on the synergistic formation of an iminoboronate structure. The PEG-b-PLys/2-FPBA micelles without CAPE are well-characterized in terms of micellization mechanism, size, morphology, pH- and GSH-responsiveness. Encapsulation of CAPE by forming PEG-b-PLys/2-FPBA/CAPE complex micelles via synergistic formation of the iminoboronate structure is discussed and pH- and GSH-responsive drug-release studies are carried out. The complex micelles are stable under physiological neutral conditions but could be destroyed in response to the stimuli of physiological acidic condition (pH 5.0) and/or glutathione (GSH) at pH 7.4. pH- and GSH-responsive drug-releases are successfully achieved. The drug-loaded complex micelles could be endocytosed by HepG2 cells and efficiently deliver hydrophilic drugs into them. This type of complex micelles could be used as a promising platform for the delivery of hydrophilic 1,2-diol-containing drugs. © The Royal Society of Chemistry.
Tian C.-F.,Hebei Medical University |
Su B.-Y.,Hebei Medical University |
Li Y.-J.,Hebei Medical University |
Tong Y.-H.,Hebei Medical University |
And 4 more authors.
Pakistan journal of pharmaceutical sciences | Year: 2016
Proper management of antibiotic-associated pseudo membranous colitis is not clear. This article is to investigate proper treatment of antibiotic-associated pseudo membranous colitis. Data of 67 patients (aged 18-69 years, with 31 males and 46 females) with antibiotic-associated pseudo membranous colitis were retrospectively analyzed including the demography, antibiotics to induce and for treatment of the pseudo membranous colitis, and other supportive measures. All 67 patients had a positive cytotoxin test, which confirmed the pseudo membranous colitis. Antibiotics which induced the pseudo membranous colitis included clindamycin, ofloxacin, piperacillin, cefatriaxone, penbritin and ceftazidime. Once the correct diagnosis was made, the culprit antibiotics were discontinued immediately, and narrow-spectrum antibiotics like metronidazole and vancomycin were administered in combination with correction of fluid and electrolyte abnormalities, use of vitamins C and B complex to repair the intestinal mucosa, and avoidance of antispasmodic and antidiarrheal agents. After appropriate treatment for 2-20 days, all patients recovered with no sequela. Sixty-two patients were clinically cured while five (7.5%) had diarrhea recurrence within two months of the end of therapy. Retreatment with tapering and extended period of metronidazole and/or vancomycin led to complete recovery of the patients. Multiple antibiotic agents are associated with pseudo membranous colitis, and correction of fluid and electrolyte abnormalities and use of vitamins to repair the intestinal mucosa should be performed to speed up the cure process.
Mi K.,Sixth Peoples Hospital Of Hebei Province |
Guo Q.,Hebei University |
Sang W.-H.,Sixth Peoples Hospital Of Hebei Province |
Wang H.-B.,Hebei University |
Li L.,Baoding First Central Hospital
Chinese Journal of Tissue Engineering Research | Year: 2016
BACKGROUND: Hyperbaric oxygen as a well-recognized therapy for ischemic and hypoxic diseases has to be combined with other treatments. OBJECTIVE: To study the effects of hyperbaric oxygen combined with neural stem cell transplantation via tail vein in chronic stress depression rats. METHODS: Of 60 Sprague-Dawley rats, 15 rats randomly selected were given no treatment as normal group, and the rest 45 ones were used to establish a rat model of depression and randomly divided into three groups: model group (n=15, without giving any treatment), neural stem cell group (n=15, injection of 1 mL neural stem cell suspension (3×106) via the tail vein) and combined group (n=15, hyperbaric oxygen treatment plus neural stem cell injection). Hyperbaric oxygen treatments were carried out four times per day, for 1 week, and all the treatments were given 24 hours after modeling. Rats in each group were subjected to body mass measurement and sugar water consumption test 1 and 2 weeks after treatment, and open field test within 5 minutes after treatment to observe rat behavior changes. The survival and distribution of CM-Dil labeled neural stem cells were observed by fluorescence microscopy. The changes in the size, morphology and number of hippocampus neurons in rat were detected by the method of nylon staining. TUNEL method was used to measure the apoptosis of nerve cells. RESULTS AND CONCLUSION: Compared with the normal group, in the model group, rat’s body weight, sucrose water preference and open field test scores were significantly lower, reduced number of hippocampal neurons with intact structure was found and TUNEL results showed more apoptosis (P < 0.05). Compared with the model group, the body weight, sucrose preference and open field test score increased significantly (P < 0.05), the number of hippocampal neurons was increased, while the number of apoptotic cells was reduced significantly in the neural stem cell and combined groups (P < 0.05). Compared with the neural stem cell group, these indexes were improved more significantly in the combined group (P < 0.05). More CM-Dil positive cells were found in the combined group than the neural stem cell group (P < 0.05). Additionally, the number of hippocampal neurons was higher in the combined group than the neural stem cell group, but lower than the normal group. To conclude, transplantation of neural stem cells combined with hyperbaric oxygen can improve the depression behavior and apoptosis in the hippocampal neurons in chronic stress depression rats. © 2016, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Li B.,Oregon Health And Science University |
Li B.,Baoding First Central Hospital |
Shi Z.,Oregon Health And Science University |
Cassaglia P.A.,Oregon Health And Science University |
Brooks V.L.,Oregon Health And Science University
Hypertension | Year: 2013
Although leptin is known to increase sympathetic nerve activity (SNA), we tested the hypothesis that leptin also enhances baroreflex control of SNA and heart rate (HR). Using α-chloralose anesthetized male rats, mean arterial pressure (MAP), HR, lumbar SNA (LSNA), splanchnic SNA (SSNA), and renal SNA (RSNA) were recorded before and for 2 hours after lateral cerebroventricular leptin or artificial cerebrospinal fluid administration. Baroreflex function was assessed using a 4-parameter sigmoidal fit of HR and SNA responses to slow ramp (3-5 minutes) changes in MAP, induced by intravenous infusion of nitroprusside and phenylephrine. Leptin (3 μg) increased (P<0.05) basal LSNA, SSNA, RSNA, HR, and MAP, and the LSNA, SSNA, RSNA, and HR baroreflex maxima. Leptin also increased gain of baroreflex control of LSNA and RSNA, but not of SSNA or HR. The elevations in HR were eliminated by pretreatment with methscopalamine, to block parasympathetic nerve activity; however, after cardiac sympathetic blockade with atenolol, leptin still increased basal HR and MAP and the HR baroreflex maximum and minimum. Leptin (1.5 μg) also increased LSNA and enhanced LSNA baroreflex gain and maximum, but did not alter MAP, HR, or the HR baroreflex. Lateral cerebroventricular artificial cerebrospinal fluid had no effects. Finally, to test whether leptin acts in the brain stem, leptin (3 μg) was infused into the 4th ventricle; however, no significant changes were observed. In conclusion, leptin acts in the forebrain to differentially influence baroreflex control of LSNA, RSNA, SSNA, and HR, with the latter action mediated via suppression of parasympathetic nerve activity. © 2013 American Heart Association, Inc.
PubMed | Baoding First Central Hospital, Beijing Institute of Pharmacology and Toxicology, Wuhan University and Chinese Institute of Basic Medical Sciences
Type: | Journal: Life sciences | Year: 2015
The aim of the study was to explore the effect of miR-200b on the development of human peripheral blood monocyte-deriveddendritic-cell (DC) and its mechanisms.Expression levels of miR-200b and its predicted targets were measured by real time-PCR. Protein expression of WASF3 was determined by Western blot and immunohistochemistry. Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque density gradient centrifugation from the buffy coat fraction of anticoagulated blood. Monocytes were purified from PBMCs using anti-CD14 microbeads. The immunophenotypes of DCs were tested by flow cytometry.A strong reduction in miR-200b expression was associated with human peripheral blood monocyte-derivedDC differentiation. The overexpression of miR-200b significantly reduced the numbers of protruding veils in mature DCs (mDCs) that are critical for promoting antigen-specificT-cell activation. Further experiments showed that miR-200b could regulate the function of DCs by targeting WASF3, a protein involved in cell movement and invasion.Our results define an important function of miR-200b in the negative regulation of DC development and provide a potential form of miRNA-mediated cell therapy for diseases that range from auto-immunity to graft-versus-host disease.
Xu Z.-W.,Hebei University |
Li Y.-Q.,Hebei University |
Liu L.-X.,Hebei University |
Zhou B.-J.,Baoding First Central Hospital
Clinical Interventions in Aging | Year: 2015
Light-chain amyloidosis is a relatively rare multisystem disorder. The disease often is normally difficult to diagnose due to its broad range of characters without specific symptoms. A 62-year-old male patient presented with heart failure after experiencing a long period of unexplained and untreated gastrointestinal symptoms. Clinical examination and laboratory findings indicated a systemic process with cardiac involvement. Echocardiography revealed concentric left ventricular hypertrophy with enhanced echogenicity and preserved ejection fraction. Rectum biopsy confirmed amyloid deposition. The side effect of delayed diagnosis on prognosis and the appropriate diagnostic strategy has been discussed. © 2015 Xu et al.
PubMed | Hebei University and Baoding First Central Hospital
Type: | Journal: Clinical interventions in aging | Year: 2015
Light-chain amyloidosis is a relatively rare multisystem disorder. The disease often is normally difficult to diagnose due to its broad range of characters without specific symptoms. A 62-year-old male patient presented with heart failure after experiencing a long period of unexplained and untreated gastrointestinal symptoms. Clinical examination and laboratory findings indicated a systemic process with cardiac involvement. Echocardiography revealed concentric left ventricular hypertrophy with enhanced echogenicity and preserved ejection fraction. Rectum biopsy confirmed amyloid deposition. The side effect of delayed diagnosis on prognosis and the appropriate diagnostic strategy has been discussed.
Li M.,Baoding First Central Hospital |
Qian Z.,Baoding Tumour Hospital |
Han X.-Y.,Baoding First Central Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2011
OBJECTIVE: To study the relationship between the expressions of Survivin and VEGF in cervical carcinoma and the clinical feature of cervical carcinoma, and to observe the correlation between Survivin and VEGF in cervical carcinoma. METHODS: Using streptavidin-biotin peroxidase (SP) method, the expressions of Survivin and VEGF were examined in 73 cases of cervical carcinoma, 25 cases of cervical intraepithelial neoplasia (CIN) and 20 cases of chronic cervicitis. RESULTS:Survivin and VEGF positive expression rate in cervical carcinoma group respectively were 75.3% and 78.1%, chronic cervical inflammation group were 5.0% and 10.0%, CIN group were 48. 0% and 52.0%. The expression of Survivin and VEGF present a growing trend from chronic cervicitis to cervical carcinoma. The expression of Survivin and VEGF were related to the clinical stages and lymph node metastasis of cervical carcinoma. A positive correlation existed among Survivin and VEGF (r = 0.738, P<0.01). CONCLUSION: The expressions of Survivin and VEGF in cervical carcinoma reflect the local invasion activity and metastasizing proficiency of cervical carcinoma, which are closely related to metastasis, clinical stages and prognosis of cervical carcinoma, and have synergistic effect in incidence and development of cervical carcinoma.
Jiang L.,Baoding First Central Hospital |
Chen H.,Baoding First Central Hospital |
Zhao W.,Baoding First Central Hospital |
Zhou B.,Baoding First Central Hospital |
Zhang J.,Baoding First Central Hospital
Cancer Research and Clinic | Year: 2014
Objective: To detect the protein expression of Cripto-1 and CD34 in basal-like breast carcinoma and study the functions of Cripto-1 in basal-like breast cancer angiogenesis, invasion and metastasis. Methods: Immunohistochemistry (IHC) was used to detect the expression of Cripto-1 and CD34 in 60 cases of basal-like breast cancer, 40 cases of non basal-like breast cancer and 20 cases of normal breast tissues. The clinical relationship between Cripto-1 and basal-like breast cancer were analyzed as well as its connection with CD34 markers MVD. Results: The expression of Cripto-1 protein in basal-like breast cancer (42/60, 70.00%) was significantly higher than that of nonbasal-like breast cancer (19/40, 47.50%) and normal breast tissue (4/20, 20.00%) (both P < 0.05). The expression of Cripto-1 protein was positively correlated with the tumor size, clinical stage, lymph nodes metastasis and MVD value in patients of basal-like breast cancer (all P < 0.05), but there was no correlation with the age of the patients (P > 0.05). Conclusions: The up-regulation of Cripto-1 expression may be associated with angiogenesis and development in basal-like breast cancer. The effect of Cripto-1 promoting angiogenesis may be an important factor in the acceleration of the local invasion and distant metastasis in basal-like breast cancer.