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Xu Z.-W.,Hebei University | Li Y.-Q.,Hebei University | Liu L.-X.,Hebei University | Zhou B.-J.,Baoding First Central Hospital
Clinical Interventions in Aging | Year: 2015

Light-chain amyloidosis is a relatively rare multisystem disorder. The disease often is normally difficult to diagnose due to its broad range of characters without specific symptoms. A 62-year-old male patient presented with heart failure after experiencing a long period of unexplained and untreated gastrointestinal symptoms. Clinical examination and laboratory findings indicated a systemic process with cardiac involvement. Echocardiography revealed concentric left ventricular hypertrophy with enhanced echogenicity and preserved ejection fraction. Rectum biopsy confirmed amyloid deposition. The side effect of delayed diagnosis on prognosis and the appropriate diagnostic strategy has been discussed. © 2015 Xu et al.

Li M.,Baoding First Central Hospital | Qian Z.,Baoding Tumour Hospital | Han X.-Y.,Baoding First Central Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To study the relationship between the expressions of Survivin and VEGF in cervical carcinoma and the clinical feature of cervical carcinoma, and to observe the correlation between Survivin and VEGF in cervical carcinoma. METHODS: Using streptavidin-biotin peroxidase (SP) method, the expressions of Survivin and VEGF were examined in 73 cases of cervical carcinoma, 25 cases of cervical intraepithelial neoplasia (CIN) and 20 cases of chronic cervicitis. RESULTS:Survivin and VEGF positive expression rate in cervical carcinoma group respectively were 75.3% and 78.1%, chronic cervical inflammation group were 5.0% and 10.0%, CIN group were 48. 0% and 52.0%. The expression of Survivin and VEGF present a growing trend from chronic cervicitis to cervical carcinoma. The expression of Survivin and VEGF were related to the clinical stages and lymph node metastasis of cervical carcinoma. A positive correlation existed among Survivin and VEGF (r = 0.738, P<0.01). CONCLUSION: The expressions of Survivin and VEGF in cervical carcinoma reflect the local invasion activity and metastasizing proficiency of cervical carcinoma, which are closely related to metastasis, clinical stages and prognosis of cervical carcinoma, and have synergistic effect in incidence and development of cervical carcinoma.

Zhang L.-N.,Hebei University | Guan H.,Baoding First Central Hospital | Yan H.,Hebei Medical University | Zhou B.-J.,Baoding First Central Hospital
Tumor | Year: 2010

Objective: To investigate the expression of insulin-like growth factor-1 receptor (IGF-1R) and IGF-2R proteins in endometrial adenocarcinoma. Methods: IGF-1R and IGF-2R expression characteristics of 20 normal endometrium tissues and 30 endometrial adenocarcinoma tissues were detected by immunohistochemistry. Results: IGF-1R overexpressian rate in the endometrial adenocarcinoma tissues was significantly higher than that in the normal endometrium tissues (P=0.006). The overall positive-expression rate and overexpression rate of IGF-2R in the endometrial adenocarcinoma tissues were significantly lower than those in the normal endometrium tissues(P=0.043, P=0.000, respectively). Conclusion: Our data suggest that increased expression of IGF-1R, together with concomitant down-regulation of IGF-2R, may be involved in the progression of endometrial adenocarcinoma.

Liu Y.,Chinese Institute of Basic Medical Sciences | Li J.,Chinese Institute of Basic Medical Sciences | Xia W.,Chinese Institute of Basic Medical Sciences | Chen C.,Chinese Institute of Basic Medical Sciences | And 12 more authors.
Life Sciences | Year: 2015

Key findings A strong reduction in miR-200b expression was associated with human peripheral blood monocyte-derivedDC differentiation. The overexpression of miR-200b significantly reduced the numbers of protruding veils in mature DCs (mDCs) that are critical for promoting antigen-specificT-cell activation. Further experiments showed that miR-200b could regulate the function of DCs by targeting WASF3, a protein involved in cell movement and invasion.Significance Our results define an important function of miR-200b in the negative regulation of DC development and provide a potential form of miRNA-mediated cell therapy for diseases that range from auto-immunity to graft-versus-host disease.Aims The aim of the study was to explore the effect of miR-200b on the development of human peripheral blood monocyte-deriveddendritic-cell (DC) and its mechanisms.Main methods Expression levels of miR-200b and its predicted targets were measured by real time-PCR. Protein expression of WASF3 was determined by Western blot and immunohistochemistry. Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque density gradient centrifugation from the buffy coat fraction of anticoagulated blood. Monocytes were purified from PBMCs using anti-CD14 microbeads. The immunophenotypes of DCs were tested by flow cytometry. © 2014 Elsevier Inc. All rights reserved.

Guo H.-Y.,Baoding First Central Hospital | Guo S.-Q.,Baoding First Central Hospital | Zhu C.-Y.,Baoding First Central Hospital | Ma J.-J.,Baoding First Central Hospital | Zhang Y.-L.,Baoding First Central Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2013

OBJECTIVE: To investigate the expression and significance of SIRT1 and its association with clinicopathologic features in endometrial carcinoma with diabetes mellitus. METHODS: Immunohistochemistry was carried out to examine the expression of SIRT1 in 39 cases of endometrial carcinoma with diabetes mellitus, 12 cases of normal endometrium with diabetes mellitus, and 40 cases of endometrial carcinoma without diabetes mellitus. Relationship between expression of SIRT1 and clinical parameters of endometrial and correlation of p53, c-erbB-2 and Ki-67 expression were analyzed. RESULTS: Expression of SIRT1 in endometrial carcinoma with diabetes mellitus was 64.1%, which was higher than that in group of normal endometrium with diabetes mellitus 8.33% (P=0.001), and lower than that of endometrial carcinoma without diabetes mellitus 85.0%(P=0.033).Expression of SIRT1 was positively correlated with p53 (r=0.360, P=0.024) and c-erbB-2(r=0.775, P<0.01), and had not correlated with Ki-67(r=0.022, P=0.896). The expression of SIRT1 was closely associated with clinical stage(P=0.023) and histologic classification(P=0.008) of endometrial carcinoma. CONCLUSIONS: Expression of SIRT1 has correlation with p53 and c-erbB-2, and SIRT1 participates glucose metabolism, the hyperexpession of SIRT1 in patients with diabetes mellitus means high risk to endometrial carcinoma. Endometrial carcinoma with diabetes mellitus can be evaluated by SIRT1 as a malignant and prognostic indicator.

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