Baoding First Central Hospital

Baoding, China

Baoding First Central Hospital

Baoding, China
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Zhang J.-J.,Peking University | Zheng Z.-Z.,Peking University | Li X.,Baoding First Central Hospital
Chinese Journal of Radiology | Year: 2010

Objective: To determine the value of MRI for diagnosing the posterior meniscus root tear. Methods: MR examinations of 30 patients with tear of the posterior meniscus root confirmed by knee arthroscopies were retrospectively reviewed. Of the 30 patients, 17 with posterior medial meniscus root tear (MMRT) and 13 with posterior lateral meniscus root tear (LMRT). The diagnostic sensitivity of MRI for the posterior meniscus root tear was analyzed. Fisher's exact test was used to compare the detection rate of MRI for MMRT with that for LMRT. Results: All 17 cases with MMRT and 9 cases out of 13 with LMRT were correctly diagnosed by MRI and the diagnostic sensitivity of MRI for the posterior meniscus root tear was 86.7% (26/30). The main MR appearance of the posterior meniscus root tear was distortion of the meniscal root, with its low signal replaced by abnormal high signal. The detection rate of MRI for MMRT (17/17) was significantly greater than that for LMRT (9/13) (P = 0.026). The prevalence of MMRT associated with meniscus extrusion (15/17) was significantly greater than that of LMRT (6/13) (P = 0.020), but the prevalence of MMRT associated with anterior cruciate ligament (ACL) injury (5/17) was significantly lower than that of LMRT (11/13) (P = 0.004). Conclusion: MRI is a relatively good method for detection of posterior meniscus root tears and associated injuries. © 2010 by the Chinese Medical Association.


Li B.,Oregon Health And Science University | Li B.,Baoding First Central Hospital | Shi Z.,Oregon Health And Science University | Cassaglia P.A.,Oregon Health And Science University | Brooks V.L.,Oregon Health And Science University
Hypertension | Year: 2013

Although leptin is known to increase sympathetic nerve activity (SNA), we tested the hypothesis that leptin also enhances baroreflex control of SNA and heart rate (HR). Using α-chloralose anesthetized male rats, mean arterial pressure (MAP), HR, lumbar SNA (LSNA), splanchnic SNA (SSNA), and renal SNA (RSNA) were recorded before and for 2 hours after lateral cerebroventricular leptin or artificial cerebrospinal fluid administration. Baroreflex function was assessed using a 4-parameter sigmoidal fit of HR and SNA responses to slow ramp (3-5 minutes) changes in MAP, induced by intravenous infusion of nitroprusside and phenylephrine. Leptin (3 μg) increased (P<0.05) basal LSNA, SSNA, RSNA, HR, and MAP, and the LSNA, SSNA, RSNA, and HR baroreflex maxima. Leptin also increased gain of baroreflex control of LSNA and RSNA, but not of SSNA or HR. The elevations in HR were eliminated by pretreatment with methscopalamine, to block parasympathetic nerve activity; however, after cardiac sympathetic blockade with atenolol, leptin still increased basal HR and MAP and the HR baroreflex maximum and minimum. Leptin (1.5 μg) also increased LSNA and enhanced LSNA baroreflex gain and maximum, but did not alter MAP, HR, or the HR baroreflex. Lateral cerebroventricular artificial cerebrospinal fluid had no effects. Finally, to test whether leptin acts in the brain stem, leptin (3 μg) was infused into the 4th ventricle; however, no significant changes were observed. In conclusion, leptin acts in the forebrain to differentially influence baroreflex control of LSNA, RSNA, SSNA, and HR, with the latter action mediated via suppression of parasympathetic nerve activity. © 2013 American Heart Association, Inc.


Du A.-J.,No 474 Hospital Of Chinese Pla | Du A.-J.,Yuncheng Central Hospital | Ren B.,No 474 Hospital Of Chinese Pla | Gao X.-W.,No 474 Hospital Of Chinese Pla | And 4 more authors.
International Journal of Ophthalmology | Year: 2013

AIM: To explore the protectiv eeffects of am inoguanidine (AG) on retinal apoptosisinmice with oxygen-induced retinopathy (OIR). METHODS: A total of 80 C5 7 BL/6J mice, aged 7 days, were randomly divided into four groups: normal, high oxygen, high oxygen saline and high oxygen treated with AG. In the normal group, mice were housed in norm oxic conditions from postnatal day P7 to P1 7. Mice in the other 3 groups were placed under hy peroxic conditions (7 5 ±2 %O2) in an oxygen-regulated chamber for 5 days and subsequently placed in normoxicconditions for 5 days. Mice in the AG group were treated once daily, from P1 2 to P1 7, with AG hem isulfate (100m g/kg body weight, intraperitoneally) dissolved in physiological saline. An equivalent amount of 0.9% phy siological saline was adm inistered, as above, to mice in the high oxygen saline group. Tenmice were randomly selected from each group on P14 and on P17, euthanized and the retinas exam ined. Apoptotic cells in the retina were detected using the term inal-deoxy nucleoitidy ltransferase mediated nick end labeling (TUNEL) method. The expression of nitric oxide synthase (iNOS) in the retina was detected by immunohistochemistry and changes in rod cells were observed using electronmicroscopy. RESULTS: TUNEL-positive cells and iNOS immunoreactive neurons were present in the inner nuclear and ganglion cell retinal lay ers of mice in the high oxygen group. The number of TUNEL-positive cells was significantly greater in the high oxygen group compared with the normal group (t=-20.81, P14d <0.05; t=-1 5.05, P17d <0.05). However, the number of TUNEL-positive cells in the AG treatment group was significantly lower (t=-13.21, P14d<0.05; t=-6.61, P 17d <0.05) compared with the high oxygen group. The expression of iNOS was significantly higher in the high oxygen group compared with the normal group (t=-21.95, P14d<0.05; t=-17.30, P17d<0.05). How ever, the expression of iNOS in the AG treatment group was significantly lower (t=-1 2.1 7, P14d<0.05; t=-1 0.30, P17d<0.05) compared with the high oxygen group. The outer segm ents of the rods were disorganized and short in the high oxygen group. Rod morphology appeared to be slightly im proved in the AG group. CONCLUSION: AG may protect retinal neurons in OIR by inhibiting apoptosis. Them echanism may be related to iNOS. © International Journal of Ophthalmology Press.


Liu Y.,Chinese Institute of Basic Medical Sciences | Li J.,Chinese Institute of Basic Medical Sciences | Xia W.,Chinese Institute of Basic Medical Sciences | Chen C.,Chinese Institute of Basic Medical Sciences | And 12 more authors.
Life Sciences | Year: 2015

Key findings A strong reduction in miR-200b expression was associated with human peripheral blood monocyte-derivedDC differentiation. The overexpression of miR-200b significantly reduced the numbers of protruding veils in mature DCs (mDCs) that are critical for promoting antigen-specificT-cell activation. Further experiments showed that miR-200b could regulate the function of DCs by targeting WASF3, a protein involved in cell movement and invasion.Significance Our results define an important function of miR-200b in the negative regulation of DC development and provide a potential form of miRNA-mediated cell therapy for diseases that range from auto-immunity to graft-versus-host disease.Aims The aim of the study was to explore the effect of miR-200b on the development of human peripheral blood monocyte-deriveddendritic-cell (DC) and its mechanisms.Main methods Expression levels of miR-200b and its predicted targets were measured by real time-PCR. Protein expression of WASF3 was determined by Western blot and immunohistochemistry. Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque density gradient centrifugation from the buffy coat fraction of anticoagulated blood. Monocytes were purified from PBMCs using anti-CD14 microbeads. The immunophenotypes of DCs were tested by flow cytometry. © 2014 Elsevier Inc. All rights reserved.


PubMed | Baoding First Central Hospital, Beijing Institute of Pharmacology and Toxicology, Wuhan University and Chinese Institute of Basic Medical Sciences
Type: | Journal: Life sciences | Year: 2015

The aim of the study was to explore the effect of miR-200b on the development of human peripheral blood monocyte-deriveddendritic-cell (DC) and its mechanisms.Expression levels of miR-200b and its predicted targets were measured by real time-PCR. Protein expression of WASF3 was determined by Western blot and immunohistochemistry. Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque density gradient centrifugation from the buffy coat fraction of anticoagulated blood. Monocytes were purified from PBMCs using anti-CD14 microbeads. The immunophenotypes of DCs were tested by flow cytometry.A strong reduction in miR-200b expression was associated with human peripheral blood monocyte-derivedDC differentiation. The overexpression of miR-200b significantly reduced the numbers of protruding veils in mature DCs (mDCs) that are critical for promoting antigen-specificT-cell activation. Further experiments showed that miR-200b could regulate the function of DCs by targeting WASF3, a protein involved in cell movement and invasion.Our results define an important function of miR-200b in the negative regulation of DC development and provide a potential form of miRNA-mediated cell therapy for diseases that range from auto-immunity to graft-versus-host disease.


Xu Z.-W.,Hebei University | Li Y.-Q.,Hebei University | Liu L.-X.,Hebei University | Zhou B.-J.,Baoding First Central Hospital
Clinical Interventions in Aging | Year: 2015

Light-chain amyloidosis is a relatively rare multisystem disorder. The disease often is normally difficult to diagnose due to its broad range of characters without specific symptoms. A 62-year-old male patient presented with heart failure after experiencing a long period of unexplained and untreated gastrointestinal symptoms. Clinical examination and laboratory findings indicated a systemic process with cardiac involvement. Echocardiography revealed concentric left ventricular hypertrophy with enhanced echogenicity and preserved ejection fraction. Rectum biopsy confirmed amyloid deposition. The side effect of delayed diagnosis on prognosis and the appropriate diagnostic strategy has been discussed. © 2015 Xu et al.


PubMed | Hebei University and Baoding First Central Hospital
Type: | Journal: Clinical interventions in aging | Year: 2015

Light-chain amyloidosis is a relatively rare multisystem disorder. The disease often is normally difficult to diagnose due to its broad range of characters without specific symptoms. A 62-year-old male patient presented with heart failure after experiencing a long period of unexplained and untreated gastrointestinal symptoms. Clinical examination and laboratory findings indicated a systemic process with cardiac involvement. Echocardiography revealed concentric left ventricular hypertrophy with enhanced echogenicity and preserved ejection fraction. Rectum biopsy confirmed amyloid deposition. The side effect of delayed diagnosis on prognosis and the appropriate diagnostic strategy has been discussed.


Li M.,Baoding First Central Hospital | Qian Z.,Baoding Tumour Hospital | Han X.-Y.,Baoding First Central Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To study the relationship between the expressions of Survivin and VEGF in cervical carcinoma and the clinical feature of cervical carcinoma, and to observe the correlation between Survivin and VEGF in cervical carcinoma. METHODS: Using streptavidin-biotin peroxidase (SP) method, the expressions of Survivin and VEGF were examined in 73 cases of cervical carcinoma, 25 cases of cervical intraepithelial neoplasia (CIN) and 20 cases of chronic cervicitis. RESULTS:Survivin and VEGF positive expression rate in cervical carcinoma group respectively were 75.3% and 78.1%, chronic cervical inflammation group were 5.0% and 10.0%, CIN group were 48. 0% and 52.0%. The expression of Survivin and VEGF present a growing trend from chronic cervicitis to cervical carcinoma. The expression of Survivin and VEGF were related to the clinical stages and lymph node metastasis of cervical carcinoma. A positive correlation existed among Survivin and VEGF (r = 0.738, P<0.01). CONCLUSION: The expressions of Survivin and VEGF in cervical carcinoma reflect the local invasion activity and metastasizing proficiency of cervical carcinoma, which are closely related to metastasis, clinical stages and prognosis of cervical carcinoma, and have synergistic effect in incidence and development of cervical carcinoma.


Jiang L.,Baoding First Central Hospital | Chen H.,Baoding First Central Hospital | Zhao W.,Baoding First Central Hospital | Zhou B.,Baoding First Central Hospital | Zhang J.,Baoding First Central Hospital
Cancer Research and Clinic | Year: 2014

Objective: To detect the protein expression of Cripto-1 and CD34 in basal-like breast carcinoma and study the functions of Cripto-1 in basal-like breast cancer angiogenesis, invasion and metastasis. Methods: Immunohistochemistry (IHC) was used to detect the expression of Cripto-1 and CD34 in 60 cases of basal-like breast cancer, 40 cases of non basal-like breast cancer and 20 cases of normal breast tissues. The clinical relationship between Cripto-1 and basal-like breast cancer were analyzed as well as its connection with CD34 markers MVD. Results: The expression of Cripto-1 protein in basal-like breast cancer (42/60, 70.00%) was significantly higher than that of nonbasal-like breast cancer (19/40, 47.50%) and normal breast tissue (4/20, 20.00%) (both P < 0.05). The expression of Cripto-1 protein was positively correlated with the tumor size, clinical stage, lymph nodes metastasis and MVD value in patients of basal-like breast cancer (all P < 0.05), but there was no correlation with the age of the patients (P > 0.05). Conclusions: The up-regulation of Cripto-1 expression may be associated with angiogenesis and development in basal-like breast cancer. The effect of Cripto-1 promoting angiogenesis may be an important factor in the acceleration of the local invasion and distant metastasis in basal-like breast cancer.


Guo H.-Y.,Baoding First Central Hospital | Guo S.-Q.,Baoding First Central Hospital | Zhu C.-Y.,Baoding First Central Hospital | Ma J.-J.,Baoding First Central Hospital | Zhang Y.-L.,Baoding First Central Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2013

OBJECTIVE: To investigate the expression and significance of SIRT1 and its association with clinicopathologic features in endometrial carcinoma with diabetes mellitus. METHODS: Immunohistochemistry was carried out to examine the expression of SIRT1 in 39 cases of endometrial carcinoma with diabetes mellitus, 12 cases of normal endometrium with diabetes mellitus, and 40 cases of endometrial carcinoma without diabetes mellitus. Relationship between expression of SIRT1 and clinical parameters of endometrial and correlation of p53, c-erbB-2 and Ki-67 expression were analyzed. RESULTS: Expression of SIRT1 in endometrial carcinoma with diabetes mellitus was 64.1%, which was higher than that in group of normal endometrium with diabetes mellitus 8.33% (P=0.001), and lower than that of endometrial carcinoma without diabetes mellitus 85.0%(P=0.033).Expression of SIRT1 was positively correlated with p53 (r=0.360, P=0.024) and c-erbB-2(r=0.775, P<0.01), and had not correlated with Ki-67(r=0.022, P=0.896). The expression of SIRT1 was closely associated with clinical stage(P=0.023) and histologic classification(P=0.008) of endometrial carcinoma. CONCLUSIONS: Expression of SIRT1 has correlation with p53 and c-erbB-2, and SIRT1 participates glucose metabolism, the hyperexpession of SIRT1 in patients with diabetes mellitus means high risk to endometrial carcinoma. Endometrial carcinoma with diabetes mellitus can be evaluated by SIRT1 as a malignant and prognostic indicator.

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