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Thongprajukaew K.,Kasetsart University | Thongprajukaew K.,Prince of Songkla University | Kovitvadhi U.,Kasetsart University | Kovitvadhi S.,Bansomdejchaopraya Rajabhat University | And 3 more authors.
Aquaculture | Year: 2011

The effects of four modified diets (gamma-irradiated, microwave-irradiated, probiotic-supplemented and carbohydrase-supplemented diets) were studied on digestive enzyme specific activities and growth performance quality of juvenile Siamese fighting fish (Betta splendens Regan, 1910) during 2. weeks of critical and intensive rearing period. The modified procedures did not change biochemical compositions and gross energy of diets, but generally resulted in relatively higher in vitro digestibilities of protein and carbohydrate and fish survival rate, albeit insignificant. Only gamma irradiation significantly increased in vitro protein digestibility of the diet, and microwave irradiation increased starch gelatinization and water solubility (P< 0.05). Fish fed microwave-treated diet showed highest values in all studied growth indicators and digestive enzyme specific activities (except lipase), with significantly higher amylase specific activity and activity ratio of amylase to trypsin (A/T ratio). Correlation analysis indicated significant relationships (P< 0.05) among the levels of total protease, amylase and trypsin, and between SGR and A/T ratio. Muscle and body compositions of juveniles fed on microwave- or gamma-irradiated diets were similar to the control, while the juveniles fed on probiotic- or carbohydrase-supplemented diets showed lower protein depositions (P< 0.05). Similar levels of RNA, RNA/Protein ratio, and Protein/Lipid ratio in body and muscle in all dietary groups fed ad libitum suggested that the improved growth performance in juvenile Siamese fighting fish fed on microwave-irradiated diet may not be only due to improved physicochemical properties of the diet but also improved fish consumption rate. © 2011 Elsevier B.V.

Nantawanit N.,Mahidol University | Chanchaichaovivat A.,Bansomdejchaopraya Rajabhat University | Panijpan B.,Mahidol University | Ruenwongsa P.,Mahidol University
Biological Control | Year: 2010

Pichia guilliermondii strain R13, a yeast isolated from Thai rambutan, has been shown to suppress the fungal pathogen Colletotrichum capsici in harvested chili. Its multiple modes of action include nutrient competition, tight attachment to the fungus, and hydrolytic enzyme secretion. This study investigated the ability of the P. guilliermondii strain R13 to induce resistance against C. capsici in chili fruit. The pretreatment of chili with the yeast antagonist, physically separated from the fungus by known distances, significantly reduced disease incidence and lesion diameter caused by C. capsici. Compared to the controls, the yeast treatment also significantly enhanced the activities of phenylalanine ammonia-lyase, chitinase, and β-1,3-glucanase, and the accumulation of capsidiol phytoalexin in chili tissue. Scanning electron micrographs showed that the morphology of C. capsici spores and hyphae were abnormal and that the pathogen had restricted growth on chili tissue adjacent to the yeast-inoculated sites. The results indicate that the induction of resistance may be another mechanism by which the yeast antagonist suppresses C. capsici. © 2009 Elsevier Inc. All rights reserved.

Congpuong K.,Ministry of Public Health | Congpuong K.,Bansomdejchaopraya Rajabhat University | Saejeng A.,Office of Disease Prevention and Control and 10 | Sug-Aram R.,Ministry of Public Health | And 4 more authors.
Malaria Journal | Year: 2012

Abstract. Background: Asymptomatic carriage of Plasmodium falciparum and Plasmodium vivax is common in both low-and high-transmission settings and represents an important reservoir of infection that needs to be targeted if malaria elimination is to succeed. Methods. Mass blood examinations (475 individuals) were conducted in two villages in Mae Hong Son, an area of endemic but low-transmission malaria in the north-west of Thailand. The microscopist at the local malaria clinic did not detect any infections. Pools of four samples were screened by real-time PCR; individual members of all of the positive pools were then re-examined by expert microscopy and by a second species-specific PCR reaction. Results: Eight subjects were found to be positive by both PCR and expert microscopy and one was found to be positive by PCR alone. The slides contained asexual stage parasites of P. vivax, P. falciparum and Plasmodium malariae, but no gametocytes. The local clinic was notified within two to eight days of the survey. Conclusion: A combination of pooling, real-time PCR and expert microscopy provides a feasible approach to identifying and treating asymptomatic malaria infections in a timely manner. © 2012 Congpuong et al.; licensee BioMed Central Ltd.

Kaewdoungdee N.,Bansomdejchaopraya Rajabhat University | Tanee T.,Mahasarakham University
Genetics and Molecular Research | Year: 2013

The Thailand cultivar pepper 'phrik man bangchang' (Capsicum annuum var. acuminatum, Solanaceae) was originally cultivated in the Bangchang Subdistrict, Amphawa District in Samut Songkhram Province. The cultivated areas are limited; we verified its distribution in Thailand for in situ 'phrik man bangchang' genetic resource conservation. Samples were collected from the original cultivation area of Bangchang Subdistrict (Or) and were randomly explored in Ratchaburi Province (RB), Khon Kaen Province (KK), and Sakon Nakhon Province (SN). A pure line from The Tropical Vegetable Research Center at Kasetsart University was used as the standard indicator. Two more Capsicum species, C. chinensis and C. frutescens, and a species from another genus in the family, Solanum melongena, were included. A dendrogram constructed from random amplified polymorphic DNA fingerprints indicated that the Or, RB, KK, and SN samples were C. annuum var. acuminatum with supportive similarity coefficients of 0.79 to 0.98. Finally, DNA barcodes, from psbA-trnH spacer region, were provided for the 3 wild species, C. annuum var. acuminatum, C. chinensis, and C. frutescens under GenBank accession Nos. JQ087869-JQ087871. The nucleotide variations between species were 0.23 to 0.26. In summary, 'phrik man bangchang' is still being planted in Bangchang Subdistrict, but only in small areas. The distribution of planting areas is expected to be throughout Thailand. © FUNPEC-RP.

Nutdanai S.,Bansomdejchaopraya Rajabhat University | Sanpanich A.,Mahidol University
BMEiCON 2014 - 7th Biomedical Engineering International Conference | Year: 2015

This research purposes to develop a medical equipment registration and spare part reserve module which is an important basic component in the WepMEt (Web application for medical equipment management in hospital) program set. This is a main assistive device for medical equipment management in a general hospital. This program is developed as a Web-base application tool which applying a decision supporting system, industrial engineering technique and experience in a real situation in a program development process. Those concepts are conformed in order obtain the program set that response to context and medical equipment management process in Thai and also concerning a hospital quality assurance. Synthesis process of main information for medical equipment registration system and spare part reserve system is performed by group criticizing of medical equipment management expertise. The program development is based on a rapid prototype technique. This program set is tested by recording of 3,512 items of medical equipment from 75 departments, 50 equipment types. The evaluation shows that our program set compatibly response to Firefox browser and able to attach electronic file into database and also able to create and print a bar-code for supporting a program operation. © 2014 IEEE.

Srisunont C.,Bansomdejchaopraya Rajabhat University | Babel S.,Thammasat University
Marine Pollution Bulletin | Year: 2015

The nutrient uptake and release by the mussels in relation with amount of food consumption are emphasised in this research. Results of the study demonstrate that about 16% of the total mass dry weight food consumed by the mussels was released as faeces. The depositions of particulate carbon, nitrogen, and phosphorus in mussel faeces were found to be 26.3, 5.7, and 0.6mg/day/indv respectively. Soluble inorganic nutrients such as NH4 +-N (2.5mg/day/indv), and PO4 3--P (0.6mg/day/indv) were also released as mussel excretion. The nutrient absorption efficiency for the green mussel body was found to be 65.1% for carbon, 62.1% for nitrogen, and 79.2% for phosphorus. Subsequently, green mussels can remove particulate carbon, nitrogen and phosphorus at 108.1, 13.5, and 4.6mg/day/indv from aquatic systems. Finally, the results can help in estimating the carrying capacity of mussel cultivation without deteriorating the water quality in marine ecosystems. © 2015 Elsevier Ltd.

Kavinseksan B.,Bansomdejchaopraya Rajabhat University | Wongsiri S.,Maejo University
Journal of Asia-Pacific Entomology | Year: 2016

The purpose of this study was to determine grooming behavior efficacy of Apis dorsata, Thai commercial (A. mellifera, Italian honey bee hybrids), and Primorsky honey bees (A. mellifera) in killing the mite species Euvarroa sinhai. Ten A. dorsata, 15 Thai commercial and 15 Primorsky colonies were used to compare efficacy in their grooming behavior related to E. sinhai. Fallen E. sinhai from A. dorsata colonies was collected using a white plastic sheet (1.4 × 2 m), which was smeared by vegetative oil and placed 1 m below each colony. The fallen E. sinhai on the plastic sheet were collected weekly until the colony was absconded. Dead E. sinhai from each colony of Thai commercial and Primorsky honey bees were collected by the use of mite collecting boards with wire screens. The boards were coated with a thin film of vegetable oil and maintained in the hives for periods of 7 days until the end of the experiment (12 months). Dead mites on the traps from A. dorsata, Thai commercial, and Primorsky colonies were retrieved by the use of a fine paint brush and examined for their injuries using a stereomicroscope at 40. × magnification. Injuries obtained by the mites were classified as either injured legs only or injured both legs and body. The data on percent means of injured mites from colonies of the three bee types were analyzed using one-way ANOVA for a completely randomized design, and the means were compared using Duncan's new multiple range test. The results showed that A. dorsata workers had more efficient grooming behavior to kill E. sinhai than the Primorsky and Thai commercial bees, respectively. The percentage of injured mites in the A. dorsata debris (55.4 ± 2.2%) was significantly higher (P = 0.001, df = 37) than that of the Thai commercial (18.6 ± 1.6%) and Primorsky (41.4 ± 2.1%) debris (percent mean ± standard error), and the percentage of injured mites in the Primorsky debris was significantly higher than that of the Thai commercial debris. The percentage of mites that had severely injured both legs and body in the A. dorsata debris (42.0%) was higher than that of the Thai commercial (31.8%) and Primorsky (36.0%) debris. Mites that had injured legs only from the A. dorsata, Thai commercial, and Primorsky debris were 58.0%, 68.2%, and 64.0%, respectively. © 2016 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.

Woods C.J.,University of Bristol | Malaisree M.,University of Bristol | Pattarapongdilok N.,Bansomdejchaopraya Rajabhat University | Sompornpisut P.,Chulalongkorn University | And 2 more authors.
Biochemistry | Year: 2012

Multidrug resistance of the pandemic H1N1-2009 strain of influenza has been reported due to widespread treatment using the neuraminidase (NA) inhibitors, oseltamivir (Tamiflu), and zanamivir (Relenza). From clinical data, the single I223R (IR(1)) mutant of H1N1-2009 NA reduced efficacy of oseltamivir and zanamivir by 45 and 10 times,(1) respectively. More seriously, the efficacy of these two inhibitors against the double mutant I223R/H275Y (IRHY(2)) was significantly reduced by a factor of 12 374 and 21 times, respectively, compared to the wild-type.(2) This has led to the question of why the efficacy of the NA inhibitors is reduced by the occurrence of these mutations and, specifically, why the efficacy of oseltamivir against the double mutant IRHY was significantly reduced, to the point where oseltamivir has become an ineffective treatment. In this study, 1 μs of molecular dynamics (MD) simulations was performed to answer these questions. The simulations, run using graphical processors (GPUs), were used to investigate the effect of conformational change upon binding of the NA inhibitors oseltamivir and zanamivir in the wild-type and the IR and IRHY mutant strains. These long time scale dynamics simulations demonstrated that the mechanism of resistance of IRHY to oseltamivir was due to the loss of key hydrogen bonds between the inhibitor and residues in the 150-loop. This allowed NA to transition from a closed to an open conformation. Oseltamivir binds weakly with the open conformation of NA due to poor electrostatic interactions between the inhibitor and the active site. The results suggest that the efficacy of oseltamivir is reduced significantly because of conformational changes that lead to the open form of the 150-loop. This suggests that drug resistance could be overcome by increasing hydrogen bond interactions between NA inhibitors and residues in the 150-loop, with the aim of maintaining the closed conformation, or by designing inhibitors that can form a hydrogen bond to the mutant R223 residue, thereby preventing competition between R223 and R152. © 2012 American Chemical Society.

Srisunont C.,Bansomdejchaopraya Rajabhat University | Babel S.,Thammasat University
Marine Pollution Bulletin | Year: 2016

This study aims to evaluate the nutrient removal potential and carrying capacity of green mussel cultivation by using the mass balance model. The developed model takes into consideration the green mussel growth rate, density and chlorophyll a concentration. The data employed in this study were based on culture conditions at Sriracha Fisheries Research Station, Thailand. Results show that net nutrient removal by green mussel is 3302, 380, and 124 mg/year/indv for carbon, nitrogen, and phosphorus respectively. The carrying capacity of green mussel cultivation was found to be 300 indv/m2 based on chlorophyll a concentration which will not release phosphorus in the water environment beyond the standard (45 μg-PO4 − 3-P/L). Higher chlorophyll a concentration results in lowered green mussel carrying capacity. This model can assist farm operators with possible management strategies for a sustainable mussel cultivation and protection of the marine environment. © 2016 Elsevier Ltd

Cheirsilp B.,Prince of Songkla University | Kitcha S.,Bansomdejchaopraya Rajabhat University
Industrial Crops and Products | Year: 2015

Lignocellulosic wastes from palm oil mill are one of attractive feedstocks for microbial lipid production by oleaginous microorganisms because of their low cost, renewable nature and abundance. In this study, four filamentous fungi with cellulolytic activity were screened as potential oleaginous microorganisms for direct conversion of these lignocellulosic wastes into lipid. Among them, Aspergillus tubingensis TSIP9 accumulated lipid at the highest amount of 39.5. ±. 2.2. mg per gram dry substrate (gds), and simultaneously produced high activities of cellulase (2.35. ±. 0.22. U/gds) and xylanase (11.83. ±. 0.18. U/gds) through solid state fermentation (SSF) of palm empty fruit bunches (EFB). The use of EFB mixed with palm kernel cake (PK) promoted lipid production by the fungi up to 79.9. ±. 3.5. mg/gds. When the enzymes were extracted from the first batch and reused in the next batch, A. tubingensis TSIP9 produced much higher amount of enzymes and accumulated lipid faster. Fed-batch SSF with intermittent adding of EFB could be applied for lipid production but with a decrease in the enzyme activity. When repeated-batch SSF with 90% replacement with new substrate was applied, both lipid and enzymes were efficiently produced for long period of fermentation. This new strategy for solid state fermentation may contribute greatly to the commercialized enzyme and lipid productions from abundant lignocellulosic biomass. © 2014 Elsevier B.V.

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