Banque Nationale de Genes

Tunis, Tunisia

Banque Nationale de Genes

Tunis, Tunisia
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Debbabi O.S.,Banque Nationale de Genes | Bouhlal R.,Institute National Of Recherches Agronomiques | Abdelaali N.,Institute National Of Recherches Agronomiques | Mnasri S.,Banque Nationale de Genes | Mars M.,University of Sousse
International Journal of Fruit Science | Year: 2013

The aim of this study was to characterize the major orange cultivars from Tunisia and to study the genetic diversity within this fruit tree species. This work was conducted in the framework of the activities of the fruit tree network in the Tunisian National Gene Bank. The pomological diversity of 28 Tunisian sweet orange cultivars was analyzed based on 18 qualitative and quantitative traits. Different regions of Tunisia, where orange cultivation is prominent, were investigated. Principal components analysis conducted on quantitative traits showed an important degree of variability of about 94% for the three first principal components. Factorial analysis of correspondence was conducted for qualitative data and it showed 69% of the total diversity for the three first factors. A dendrogram was constructed based on average distances. Results showed that the geographic origin was not the determinant criteria for cultivar segregation. © 2013 Copyright Taylor and Francis Group, LLC.

Nasri N.,University of Tunis | Nasri N.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Elfalleh W.,Institute des Regions Arides | Tlili N.,University of Tunis | And 4 more authors.
Lipids in Health and Disease | Year: 2012

Background: Oilseed samples from four Acacia species (A. cyclops, A. ligulata, A. salicina and A. cyanophylla) were analyzed in order to evaluate the potential nutritional value of their unexploited seeds. Methods: Samples were collected from different Tunisian geographic locations. Seed oils were extracted and carotenoids, tocopherols and sterols were analyzed using chromatographic methods. Results: The studied Acacia seeds seem to be quite rich in lipids (from 6% to 12%). All Acacia species contain mainly the xanthophylls zeaxanthin and lutein compounds: from ca. 38 of total lipids (A. cyclops) to ca. 113 of total lipids (A. cyanophylla). Total tocopherols varied from ca. 221 of total lipids (A. cyclops) to ca. 808 of total lipids (A. ligulata). Sterols are highly present and their contents ranged between ca. 7 g. kg-1 of total lipids (A. salicina) and 11 g. kg-1 of total lipids (A. cyclops). Conclusion: This study highlights that these unexploited seeds might have a potential nutritional value and encourages researchers to more explore and find developments for these plants for healthy purposes. © 2012 Nasri et al.; licensee BioMed Central Ltd.

Mezghani N.,Banque Nationale de Genes | Zaouali I.,Banque Nationale de Genes | Amri W.B.,Banque Nationale de Genes | Rouz S.,Institute Superieur Agronomique Of Mogran | And 6 more authors.
Genetic Resources and Crop Evolution | Year: 2014

Fruits present major morphological characters used to define genera and species within the Apiaceae. Northern Africa represents a major center of diversity of Daucus, with Tunisia containing at least 12 species and six subspecies. We assessed 14 mature fruit characters from the Daucus L. germplasm collection at the National Gene Bank of Tunisia. Quantification of variability for each character was investigated using the standardized Shannon-Weaver diversity index (H′). Diversity was established by factorial analysis of correspondence and cluster analysis. The computing H′ index ranged from 0.31 for stylopodium shape to a maximum of 0.81 for spine shape. A mean diversity index for all traits recorded across all populations averaged 0.58 indicating existence of an important genetic diversity within the collection. Multivariate analysis of factorial correspondence and cluster analysis on morphological descriptors permitted the subdivision of the Daucus collection into five distinct groups including one single accession group, two groups with six accessions, one group of nine accessions and one large group with 81 accessions corresponding each one to a species among Daucus except the large group corresponding to D. carota and D. capillifolius. The grouping of populations did not reflect bioclimatic and geographic patterns, suggesting adaptation of populations to local environments. Of equal importance, our study shows the effectiveness of fruit characters alone to identify species in this collection of Daucus. © Springer Science+Business Media Dordrecht (outside the USA) 2013.

Sayar R.,National Agriculture Research Institute of Tunisia INRAT | Bchini H.,Physiology Laboratory | Mosbahi M.,Banque Nationale de Genes | Ezzine M.,Physiology Laboratory
African Journal of Agricultural Research | Year: 2010

The present investigation has been performed to evaluate Durum wheat (Triticum durum Desf.) tolerance to osmotic stress at the water potentials of -0.2, -0.4, -0.6 and -0.8 MPa induced by polyethylene glycol PEG-8000 (polyethylene glycol 8000) or NaCI solutions at an early stage of plant growth. Each, on germination, emergence and early seedling growth, were investigated for two Durum wheat (Triticum durum Desf.) varieties differing in drought tolerance (BD290273 and Omrabia). Daily and final germination and emergence percentage, as well as germination and seedling emergence rate, and seedling fresh and dry weight were measured in the study under controlled conditions. Results showed those germination and emergence rates were delayed by both solutions in both varieties, with differences between genotypes among growth stages, given that Omrabia variety showed a higher germination and emergence rate than BD290273 genotype in NaCI but BD290273 was less affected by NaCI and PEG solutions at the emergence stage. Sodium chloride had a lesser effect on varieties in terms of germination and emergence rates, the final germination and the emergence percentage than did PEG-8000. This conclusively proves that the adverse effect of PEG on germination, emergence and early seedling growth was due to the osmotic effect rather than the specific ion. Seedling growth was reduced by both stresses. But NaCI usually caused less damage than PEG to Durum wheat seedlings, suggesting that NaCI and PEG acted through different mechanisms. This difference in cultivar's behaviour according to the growth conditions is discussed. It was concluded that inhibition in germination at equivalent water potentials of NaCI and PEG-8000 was mainly due to an osmotic effect rather than did salt toxicity. ©2010 Academic Journals.

Ghrabi-Gammar Z.,Banque Nationale de Genes | Ghrabi-Gammar Z.,Manouba University | Ben Haj Jilani I.,Manouba University | Ben Haj Jilani I.,Institute National Agronomique Of Tunisie | And 4 more authors.
Acta Horticulturae | Year: 2010

Ghomrassen is a rustic Berber village in south-eastern Tunisia, located 16 km from Tataouine and 40 km from Médenine. It is a municipality about 12000 inhabitants. Allagua is a very ancient Tunisian tradition especially famous in southern Tunisia. The term Allagua in the Ghomrassen region refers to a basket containing several objects (items), especially related to beauty and medicinal usages, that the new husband offers to his future wife during wedding festivities. This tradition dates to the time when the elementary care could be made only by the traditional medicine. The objective of our work is to collect information, so far untranscribed relating to the characteristics and use of plant-derived and other Allagua items, including the used plant organ, the origin, the type and mode of usage. An ethnobotanic survey was conducted in the Ghomrassen region during summer 2007, involving 20 resource persons (5 storekeepers, 5 chemists or plantssellers and 10 elderly women) known as having a deep knowledge of wedding ceremony traditions at Ghomrassen. Survey data were collected and analyzed, covering every inventoried species. More than 36 different articles were inventoried, including 10 of mineral origin and 20 of plant origin. The plant-originating items make up approximately 56% of all Allagua items. They belong to 20 different genuses in 18 families. The most important species are: Prunus mahaleb, Lawsonia inermis, Santalum album, Myrtus communis, Chamaeleon gummifer, Juglans regia, Atractylis gummifera. The majority of inventoried items are not exploited as raw materials. In fact, 15 items are used as ingredients of several special preparations including the Skhab preparation (made up of 9 items), the Henna and Bkhour (each made up also of 9 different items), the Mardouma (4 items) and the Kohl (2 items). Ten items articles belonging to 10 different plants are used for medicinal purposes and are generally present in the Allagua of the future bride. However, 80% of the surveyed persons indicated that the medicinal usage of these items is more and more abandoned, and they are less and less supplied in the Allagua. The tradition of necklace making is still transmitted from one generation to another and research studies on improving paste quality (colour, consistency, smell) are conducted by the National Office of Crafts. Today, several traditional and modern craft creations are marketed. On the other hand, Allagua components of medicinal usage are more and more abandoned in spite of the scientific validation of such usage.

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