Bangladesh Rice Research Institute BRRI

Gazipur, Bangladesh

Bangladesh Rice Research Institute BRRI

Gazipur, Bangladesh
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Ashkani S.,University Putra Malaysia | Ashkani S.,Islamic Azad University at Tehran | Rafii M.Y.,University Putra Malaysia | Rahim H.A.,Malaysian Nuclear Agency | And 2 more authors.
Molecular Biology Reports | Year: 2013

Rice blast is one of the major fungal diseases that badly reduce rice production in Asia including Malaysia. There is not much information on identification of QTLs as well as linked markers and their association with blast resistance within local rice cultivars. In order to understanding of the genetic control of blast in the F3 families from indica rice cross Pongsu seribu2/Mahsuri, an analysis of quantitative trait loci against one of the highly virulent Malaysian rice blast isolate Magnaporthe oryzae, P5.0 was carried out. Result indicated that partial resistance to this pathotype observed in the present study was controlled by multiple loci or different QTLs. In QTL analysis in F3 progeny fifteen QTLs on chromosomes 1, 2, 3, 5, 6, 11 and 12 for resistance to blast nursery tests was identified. Three of detected QTLs (qRBr-6.1, qRBr-11.4, and qRBr-12.1) had significant threshold (LOD >3) and approved by both IM and CIM methods. Twelve suggestive QTLs, qRBr-1.2, qRBr-2.1, qRBr-4.1, qRBr-5.1, qRBr-6.2, qRBr-6.3, qRBr-8.1, qRBr-10.1, qRBr-10.2, qRBr-11.1, qRBr-11.2 and qRBr-11.3) with Logarithmic of Odds (LOD) <3.0 or LRS <15) were distributed on chromosomes 1, 2, 4, 5, 6, 8, 10, and 11. Most of the QTLs detected using single isolate had the resistant alleles from Pongsu seribu 2 which involved in the resistance in the greenhouse. We found that QTLs detected for deferent traits for the using isolate were frequently located in similar genomic regions. Inheritance study showed among F3 lines resistance segregated in the expected ratio of 15: 1 for resistant to susceptible. The average score for blast resistance measured in the green house was 3.15, 1.98 and 29.95 % for three traits, BLD, BLT and % DLA, respectively. © 2012 Springer Science+Business Media Dordrecht.

Ashkani S.,University Putra Malaysia | Ashkani S.,Islamic Azad University at Tehran | Rafii M.Y.,University Putra Malaysia | Rahim H.A.,Malaysian Nuclear Agency | And 2 more authors.
Biotechnology Letters | Year: 2013

Malaysian rice, Pongsu Seribu 2, has wide-spectrum resistance against blast disease. Chromosomal locations conferring quantitative resistance were detected by linkage mapping with SSRs and quantitative trait locus (QTL) analysis. For the mapping population, 188 F3 families were derived from a cross between the susceptible cultivar, Mahsuri, and a resistant variety, Pongsu Seribu 2. Partial resistance to leaf blast in the mapping population was assessed. A linkage map covering ten chromosomes and consisting of 63 SSR markers was constructed. 13 QTLs, including 6 putative and 7 putative QTLs, were detected on chromosomes 1, 2, 3, 5, 6, 10, 11 and 12. The resulting phenotypic variation due to a single QTL ranged from 2 to 13 %. These QTLs accounted for approx. 80 % of the total phenotypic variation within the F3 population. Therefore, partial resistance to blast in Pongsu Seribu 2 is due to combined effects of multiple loci with major and minor effects. © 2013 Springer Science+Business Media Dordrecht.

Mazid M.S.,University Putra Malaysia | Rafii M.Y.,University Putra Malaysia | Hanafi M.M.,University Putra Malaysia | Rahim H.A.,Malaysian Nuclear Agency | And 3 more authors.
South African Journal of Botany | Year: 2013

Genetic based knowledge of different growth traits including morphological, physiological and developmental plays fundamental role in the improvement of rice. Genetic divergence allows superior recombinants which are essential in any crop development project. Forty-one rice genotypes including bacterial blight (BB) resistant and susceptible checks were assessed for 13 morphological traits. Among the genotypes, almost all the traits exhibited highly significant variation. The higher extent of genotypic (GCV) as well as phenotypic coefficients of variation (PCV) were noticed for number of tillers hill-1, total number of spikelets panicle-1, number of filled grains panicle-1, and yield hill-1. High heritability together with high genetic advance was observed for total number of spikelets panicle-1, number of filled grains panicle-1, and yield hill-1 indicating dominant role of additive gene action in the expression of these traits. Number of filled grains panicle-1 exhibited positive correlation with most of the traits. Yield hill-1 showed a good number of highly significant positive correlations with number of filled grain panicle-1, total number of spikelets panicle-1, 1000 grain weight hill-1, number of panicle hill-1, and panicle length. The UPGMA dendrogram divided all the genotypes in to six major clusters. The PCA showed 13 morphological traits generated about 71% of total variation among all the genotypes under this study. On the basis of 13 morphological traits, genotypes such as IRBB2, IRBB4, IRBB13, IRBB21, and MR263 could be hybridized with genotypes MR84, MR159, MRQ50, MRQ74, PH9 and IR8 in order to develop suitable BB resistant rice genotypes. © 2013 South African Association of Botanists.

Hashemi F.S.G.,University Putra Malaysia | Rafii M.Y.,University Putra Malaysia | Ismail M.R.,University Putra Malaysia | Mahmud T.M.M.,University Putra Malaysia | And 6 more authors.
Critical Reviews in Plant Sciences | Year: 2013

One of the most valuable traits in high-quality rice is aroma or fragrance, which is important for consumer preference and global trade. Aromatic rice is unique and recognized as a badge of honor and an asset in many countries. Among more than 100 volatile components, 2-acetyl-1-pyrroline (2AP) is believed to be the main aromatic compound in rice. The principal gene contributing to 2AP is badh2, which was mapped on chromosome 8 by map-based cloning. A deletion in this gene truncates and makes non-functional the BADH2 protein. Thus, the mutant badh2 transcript leads to 2AP accumulation in aromatic rice. The discovery of the gene has led to the clarification of the biochemistry, molecular genetics and evolution of fragrant rice. The breeding of fragrant rice is now faster because of marker assisted selection (MAS), which is based on recognized genes. For a more extensive elucidation of all effective and fundamental factors contributing to rice fragrance, it is essential to further explore target quantitative trait loci (QTLs) and their inheritance and locations. © 2013 Copyright Taylor and Francis Group, LLC.

Ashkani S.,University Putra Malaysia | Ashkani S.,Islamic Azad University at Tehran | Rafii M.Y.,University Putra Malaysia | Shabanimofrad M.,University Putra Malaysia | And 4 more authors.
Critical Reviews in Biotechnology | Year: 2016

Rice blast disease, which is caused by the fungal pathogen Magnaporthe oryzae, is a recurring problem in all rice-growing regions of the world. The use of resistance (R) genes in rice improvement breeding programmes has been considered to be one of the best options for crop protection and blast management. Alternatively, quantitative resistance conferred by quantitative trait loci (QTLs) is also a valuable resource for the improvement of rice disease resistance. In the past, intensive efforts have been made to identify major R-genes as well as QTLs for blast disease using molecular techniques. A review of bibliographic references shows over 100 blast resistance genes and a larger number of QTLs (∼500) that were mapped to the rice genome. Of the blast resistance genes, identified in different genotypes of rice, ∼22 have been cloned and characterized at the molecular level. In this review, we have summarized the reported rice blast resistance genes and QTLs for utilization in future molecular breeding programmes to introgress high-degree resistance or to pyramid R-genes in commercial cultivars that are susceptible to M. oryzae. The goal of this review is to provide an overview of the significant studies in order to update our understanding of the molecular progress on rice and M. oryzae. This information will assist rice breeders to improve the resistance to rice blast using marker-assisted selection which continues to be a priority for rice-breeding programmes. © 2014 Informa Healthcare USA, Inc.

Dutta A.K.,Bangladesh Rice Research Institute BRRI | Dutta A.K.,University of Dhaka | Gope P.S.,Center for Food and Waterborne Diseases | Banik S.,Bangladesh Rice Research Institute BRRI | And 3 more authors.
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To study total phenolic content and antioxidant properties of 80% methanol extracts of ten high yielding rice varieties, five each from two different seasons namely aman and boro of Bangladesh. Methods: Total phenolic content was measured by Folin-Ciocalteau method while 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, hydroxyl ion scavenging, ferric reducing antioxidant power (Ferric reducing antioxidant power), and total antioxidant capacity (TAC) by ammonium molybdate, were used to analyze their Antioxidant properties. Results: Rice variaty BR5 of aman and BRRI dhan28 of boro season comparatively showed higher TPC and Antioxidant properties than the other rice varieties. BR22 of aman season showed the highest hydroxyl ion scavenging activity although it displayed the lowest TPC. Except for hydroxyl ion scavenging activity, aman rice varieties displayed comparatively higher total phenolic content and antioxidant property than the boro rice varieties. Conclusions: The results of the present study implies that the selected rice varieties possess moderate antioxidant capacity and therefore, can be considered as health supplements and nutraceuticals foods as rice is the staple food of Bangladesh. © 2012 Asian Pacific Tropical Biomedical Magazine.

Bari M.N.,Bangladesh Rice Research Institute BRRI | Jahan M.,Bangladesh Agricultural University | Islam K.S.,Bangladesh Agricultural University
Environmental Entomology | Year: 2015

The influence of different temperatures on biological parameters of native strains of Trichogramma zahiri Polaszek (Hymenoptera: Trichogrammatidae), an egg parasitoid of rice hispa, Dicladispa armigera (Olivier) (Chrysomelidae: Coleoptera), was evaluated in the laboratory on its host. The key biological parameters of the parasitoid T. zahiri in relation to temperature were investigated to find out its candidature as a potential biological control agent of rice hispa. The highest number of eggs parasitized by T. zahiri was 15.7 eggs per female at 26°C, which differed significantly from those at 18, 22, 30, and 34°C (P<0.05). Development duration and longevity of T. zahiri decreased as temperature increased. Fecundity differed significantly at all constant temperatures. Emergence rates decreased at both high (34°C) and low (<26°C) temperatures. Female-biased sex ratio ranged from 54 to 70% at all constant temperatures. The lower temperature threshold for T. zahiri was 6.2°C for males and 6.95°C for females. The upper threshold temperatures were 35.82 and 35.87°C for males and females, respectively. Net reproductive rate (R0) was highest at 26°C compared with other temperatures. Mean cohort generation time (tG) and population doubling time (tD) decreased as temperature increased from 18 to 30°C. The daily intrinsic rate of increase (rm) and finite rate of increase were positively correlated with temperatures ranging from 18 to 30°C and then decreased at 34°C. The relevance of our results is discussed in the context of climatic adaptation and biological control. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved.

Rashid M.M.,Bangladesh Rice Research Institute BRRI | Jahan M.,Bangladesh Agricultural University | Islam K.S.,Bangladesh Agricultural University
Neotropical Entomology | Year: 2016

Nitrogen (N) limitation is well documented for the brown planthopper (BPH) Nilaparvata lugens (Stål), but phosphorus (P) and potassium (K) limitation is poorly studied. We studied the effects of N, P, and K application on chemical composition of rice plants and its consequences on life parameters—adult longevity, fecundity, and egg hatchability of BPH. Life parameters of BPH were regressed as function of plant chemical composition. A completely randomized design with four replicates in a factorial scheme was used considering N, P, and K levels as factors. Nitrogen application increased N and soluble proteins (SP) and decreased silicon (Si) content in the plants resulting in increased adult longevity, fecundity, and egg hatchability of BPH. Phosphorus fertilization increased P content and showed markedly increased fecundity, but not egg hatchability or adult longevity. Significant interaction between N and P was observed for fecundity of BPH. Potassium supplementation increased K content but reduced N, Si, SP, and total free sugars (TFS) content in the plants, but it had no significant effect on life parameters of BPH. The association of BPH life parameters with N, SP, TFS, and P content was significant and positive, but it was negative with the content of Si. Thus, N and P fertilization on rice plants enhanced BPH fitness. In conclusion, judicious nutrient application can be helpful in avoiding generalized infestation of BPH to rice. © 2016, Sociedade Entomológica do Brasil.

Rafii M.Y.,University Putra Malaysia | Shabanimofrad M.,University Putra Malaysia | Puteri Edaroyati M.W.,University Putra Malaysia | Latif M.A.,University Putra Malaysia | Latif M.A.,Bangladesh Rice Research Institute BRRI
Molecular Biology Reports | Year: 2012

A sum of 48 accessions of physic nut, Jatropha curcas L. were analyzed to determine the genetic diversity and association between geographical origin using RAPD-PCR markers. Eight primers generated a total of 92 fragments with an average of 11.5 amplicons per primer. Polymorphism percentages of J. curcas accessions for Selangor, Kelantan, and Terengganu states were 80.4, 50.0, and 58.7%, respectively, with an average of 63.04%. Jac-card's genetic similarity co-efficient indicated the high level of genetic variation among the accessions which ranged between 0.06 and 0.81. According to UPGMA dendrogram, 48 J. curcas accessions were grouped into four major clusters at coefficient level 0.3 and accessions from same and near states or regions were found to be grouped together according to their geographical origin. Coefficient of genetic differentiation (G st) value of J. curcas revealed that it is an outcrossing species. © 2012 Springer Science+Business Media B.V.

Azizur Rahman M.,Kanazawa University | Mamunur Rahman M.,Kanazawa University | Mamunur Rahman M.,Bangladesh Rice Research Institute BRRI | Kadohashi K.,Kanazawa University | And 2 more authors.
Chemosphere | Year: 2011

This study was conducted to investigate the effect of external iron status and arsenic species on chelant-enhanced iron bioavailability and arsenic uptake. Rice seedlings (Oryza sativa L.) were used as model plant, and were grown in artificially contaminated sandy soils irrigated with Murashige and Skoog (MS) culture solution. Arsenate uptake in roots and shoots of rice seedlings were affected significantly (p>0.05) while dimethylarsinic acid (DMAA) was not by the additional iron and chelating ligand treatments. Regardless of iron concentrations in the soil solution, HIDS increased arsenic uptake for roots more than EDTA and EDDS. Chelating ligands and arsenic species also influenced iron uptake in rice roots. Irrespective of arsenic species, HIDS was found to be more effective in the increase of iron bioavailability and uptake in rice roots compared to other chelants. There was a significant positive correlation (r=0.78, p<0.05) between arsenate and iron concentrations in the roots of rice seedlings grown with or without additional iron indicating that arsenate inhibit iron uptake. In contrast, there was no correlation between iron and DMAA uptake in roots. Poor correlation between iron and arsenic in shoots indicated that iron uptake in shoots was neither affected by additional iron nor by arsenic species. Compared to the control, chelating ligands increased iron uptake in shoots of rice seedlings significantly (p<0.05). Regardless of additional iron and arsenic species, iron uptake in rice shoots did not differed among EDTA, EDDS, and HIDS treatments. © 2011 Elsevier Ltd.

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