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Sarker S.K.,Bangladesh Livestock Research Institute | Yang C.J.,Sunchon National University
Journal of Medicinal Plant Research | Year: 2011

A study was conducted with 140 "Ross" broilers (1-day old) having four treatments to know the potentiality of Eosungcho, Houttuynia cordata with probiotics (HCP) and suitable level in replacing antibiotic. The groups were control (basal diet), antibiotic (basal diet + 0.05% OTC) and HCP 0.5 and 1.0% with basal diet. The birds were arranged in a completely randomized design having 5 replications with 7 chicks per replication in wire cage. Addition of feed additives in the diet did not show negative effect on growth and feed conversion efficiency in broilers. Significantly, highest protein percentage in broiler meat fed 1.0% HCP was recorded compared to control (p<0.05). Crude fat significantly reduced in 1.0% HCP like antibiotic compared to control group (p<0.05). The thiobarbituric acid (TBA) value of meat in antibiotic and HCP groups showed significantly lower value compared to control (p<0.05). No mortality was observed in 1.0% HCP added diet compared to other groups. Although, no difference in internal organs among the groups were observed except large intestine weight. Significantly, lowest large intestinal weight was found in antibiotic and 1.0% HCP addition diet were found (p<0.05). Considering the findings, addition of 1.0% Eusungcho (Houttuynia cordata) with multi strain probiotics can be replaceable to antibiotic for broiler production. © 2011 Academic Journals.


Sarker M.S.K.,Sunchon National University | Yang C.J.,Bangladesh Livestock Research Institute
Journal of Animal and Veterinary Advances | Year: 2010

A study was conducted with 48 Hanwoo post weaning calves from 91 -120 days to know the effect of natural feed additives on growth, feed intake and blood profiles. The calves were arranged in four groups; control, antibiotic (Neomycine 110 ppm), propolis (0.05%) and illite (2%) having 12 calves (male female ratio 6:6) in each. The claves were supplied pellet feeds ad libitum. Multi nutrient block and timothy grass hay were supplied to the calves. Water was supplied ad libitum through the water tub. Significantly lowest (p<0.05) initial BW was recorded at the beginning of post-weaning age in propolis group compared to others. The ADG at 5 months age were found statistically similar among the groups which indicates the effectiveness of the propolis. Higher feed intakes were recorded in control and propolis group followed by antibiotic and illite group. Illite and propolis group showed better compared to control diet in terms of ADG and feed efficiency. Addition of propolis and illite in the diet of post-weaning calves increased the percentage of platelets count (p<0.05) keeping other blood component statistically unchanged. The serum albumin and globulin values also falls within the range of antibiotic group although lowest IgM was recorded in illite group. In conclusion, the natural resources, illite and propolis showed potential without any detrimental effect for production of Hanwoo post-weaning calves. © Medwell Journals, 2010.


Ahmed S.S.U.,Copenhagen University | Ahmed S.S.U.,Chittagong Veterinary and Animal Sciences University | Themudo G.E.,University of Southern Denmark | Christensen J.P.,Copenhagen University | And 5 more authors.
Vaccine | Year: 2012

Bangladesh has been severely hit by highly pathogenic avian influenza H5N1 (HPAI-H5N1). However, little is known about the genetic diversity and the evolution of the circulating viruses in Bangladesh. In the present study, we analyzed the hemagglutinin gene of 30 Bangladeshi chicken isolates from 2007 through 2010. We analyzed the polybasic amino acid sequence motif of the cleavage site and amino acid substitution pattern. Phylogenetic history was reconstructed using neighbor-joining and Bayesian time-scaled methods. In addition, we used Mantel correlation tests to analyze the relation between genetic relatedness and spatial and temporal distances. Neighbor-joining phylogeography revealed that virus circulating in Bangladesh from 2007 through 2010 belonged to clade 2.2. The results suggest that clade 2.2 viruses are firmly entrenched and have probably become endemic in Bangladesh. We detected several amino acid substitutions, but they are not indicative of adaptation toward human infection. The Mantel correlation test confirmed significant correlation between genetic distances and temporal distances between the viruses. The Bayesian tree shows that isolates from waves 3 and 4 derived from a subgroup of isolates from the previous waves grouping with a high posterior probability (pp = 1.0). This indicates the possibility of formation of local subclades. One surprising finding of spatio-temporal analysis was that genetically identical virus caused independent outbreaks over a distance of more than 200 km and within 14 days of each other. This might indicate long distance dispersal through vectors such as migratory birds and vehicles, and challenges the effectiveness of movement restriction around 10 km radius of an outbreak. The study indicates possible endemicity of the clade 2.2 HPAI-H5N1 virus in Bangladesh. Furthermore, the formation of a subclade capable of transmission to humans cannot be ruled out. The findings of this study might provide valuable information for future surveillance, prevention and control programme. © 2012 Elsevier Ltd.


Choi B.-H.,Gyeongsang National University | Bang J.-I.,Gyeongsang National University | Jin J.-I.,Gyeongsang National University | Kim S.-S.,Gyeongsang National University | And 6 more authors.
Theriogenology | Year: 2013

Oocyte quality is a key factor affecting success of invitro embryo production in cattle. Improving the microenvironment of oocytes during invitro maturation (IVM) can increase developmental rate and embryo quality. Therefore, the objective was to determine whether denuded oocytes (DO) affect embryo development and ultrastructure of the zona pellucida (ZP) in invitro matured bovine oocytes. Intact immature cumulus-oocytes complexes (COC) obtained from a local abattoir or by ovum pick-up (OPU) were cocultured with and without abattoir-obtained DO at a COC:DO ratio of 1:5. After IVM, DO were removed and intact DO were either fertilized or observed by scanning electron microscopy. Blastocyst quality was evaluated using a TUNEL assay. The ZP pore size decreased after IVM in COC + DO coculture, regardless of their origin (OPU, 310.5 ± 92.5 vs. 428.9 ± 148.5 nm; abattoir, 317.5 ± 68.5 vs. 358.9 ± 128.5 nm; P < 0.05; mean values ± standard deviation). Moreover, the number of ZP pores in OPU COC + DO and COC + DO was greater than those in OPU COC and COC (control) groups (56 ± 4 and 55 ± 7 vs. 50 ± 6 and 42 ± 4; P < 0.05). The rate of blastocyst development in COC + DO and OPU COC + DO groups was greater those in control and OPU COC groups (36.6% and 55.5% vs. 28.1% and 40.0%; P < 0.05). Moreover, the total cell numbers of blastocysts in COC + DO group exceeded that of control (132.91 ± 30.90 vs. 115.44 ± 24.95; P < 0.05), with no significant between OPU COC + DO and OPU COC groups (139.31 ± 42.51 vs. 137.00 ± 61.34). In conclusion, invitro embryo development competence and quality improved when oocytes were cocultured with DO. Furthermore, there more, but smaller, ZP pores. © 2013 Elsevier Inc.


Sarkerand M.S.K.,Bangladesh Livestock Research Institute | Yang C.J.,Sunchon National University
Journal of Animal and Veterinary Advances | Year: 2010

A study was conducted with 48 Hanwoo calves from birth to 90 days to know the effect of natural feed additives on growth, feed intake and blood profiles. The calves were arranged in four groups; control, antibiotic (Neomycine 110 ppm), propolis (0.05%) and illite (2%) having 12 calves (male female ratio 6:6) in each. The claves were supplied pellet feeds ad libitum. They were allowed to suck mother's milk and timothy grass hay were supplied after 1 month and multi nutrient block. Water was supplied ad libitum through the water tub. The significantly highest (p<0.05) BW and ADG were found in antibiotic fed calves than other groups. There were almost similar values observed in control and illite fed calves. Significant lowest (p<0.05) weight was observed in propolis fed calves. Highest feed intake was recorded in control group followed by antibiotic, illite and propolis group. Illite group seems to be suitable among the feed additives groups but the daily weight gam was statistically lowest (p<0.05). Blood profiles didn't show any effects after addition of additives except significant reduction of A/G ratio. So considering all the factors both the natural resources, Illite and Propolis didn't show potential for production of Hanwoo calf at pre-weaning. © Medwell Journals, 2010.

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