Bangladesh Council of Scientific and Industrial Research Laboratories

Rājshāhi, Bangladesh

Bangladesh Council of Scientific and Industrial Research Laboratories

Rājshāhi, Bangladesh
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Ahmed F.R.S.,Bangladesh University | Amin R.,Bangladesh Council of Scientific and Industrial Research Laboratories | Hasan I.,Bangladesh University | Asaduzzaman A.K.M.,Bangladesh University | Kabir S.R.,Bangladesh University
International Journal of Biological Macromolecules | Year: 2017

A lectin was isolated from the tuberous rhizome of Keampferia rotunda by using different chromatographic methods with the molecular weight of 21 ± 1 kDa. The lectin contained highest percentage of leucine and lowest percentage of tryptophan residues as determined by LC–MS. The lectin agglutinated mice and human erythrocytes and the hemagglutination activity was inhibited by Methyl-β-D-galactopyranoside. The lectin did not lose its activity in the presence of urea but the activity abolished completely when treated with EDTA. The lectin exhibited its activity at the pH ranging from 6.0 to 9.0 and in a temperature range of 30–80 °C. Antiproliferative activity was studied against Ehrlich ascites carcinoma (EAC) and U87 cell lines. No inhibitory effect was observed against U87 cell line whereas 43.7% cell growth inhibition was observed in vitro against EAC cells at 160 μg/ml. The lectin was injected (i.p.) in EAC bearing Swiss albino mice at the doses of 3.0 and 6.0 mg/kg/day for five consecutive days and 41 and 59% of EAC cell growth inhibition was observed, respectively. The cell growth inhibition was due to the induction of apoptosis in the EAC cells which was confirmed by cell morphological study, caspase-3 inhibitor and apoptosis-related gene expression. © 2017 Elsevier B.V.


Ali G.,Asian Institute of Technology | Nitivattananon V.,Asian Institute of Technology | Molla N.A.,Bangladesh Council of Scientific and Industrial Research Laboratories | Hussain A.,Asian Institute of Technology
World Academy of Science, Engineering and Technology | Year: 2010

Solid waste management is a vital issue to reduce, manage, segregate, dispose off properly and it involves more management, skills, and techniques when it is being involved in so many types of infected waste materials. The paper in hand depicts the solid waste management in Thammasat hospital located in Pathumthani Municipality, Thailand through neoteric methodology to select alternative technology. Hospital waste generation rate is 1200 kg per day approximately and the cost of waste collection is more than 3 millions almost annually. Waste disposing is the main problem for the hospital and this study attempted to suggest appropriate technology to resolve this problem and suggested best waste management technology. The results showed that two technologies can be selected to dispose the waste i.e. incineration and sanitary landfill and among these two incineration is the best.


Kabir S.R.,University of Rajshahi | Rahman M.M.,University of Rajshahi | Tasnim S.,University of Rajshahi | Karim M.R.,University of Rajshahi | And 10 more authors.
International Journal of Biological Macromolecules | Year: 2016

Chitinases are a group of enzymes that show differences in their molecular structure, substrate specificity, and catalytic mechanism and widely found in organisms like bacteria, yeasts, fungi, arthropods actinomycetes, plants and humans. A novel chitinase enzyme (designated as TDSC) was purified from Trichosanthes dioica seed with a molecular mass of 39±1kDa in the presence and absence of β-mercaptoethanol. The enzyme was a glycoprotein in nature containing 8% neutral sugar. The N-terminal sequence was determined to be EINGGGA which did not match with other proteins. Amino acid analysis performed by LC-MS revealed that the protein was rich in leucine. The enzyme was stable at a wide range of pH (5.0-11.0) and temperature (30-90°C). Chitinase activity was little bit inhibited in the presence of chelating agent EDTA (ethylenediaminetetraaceticacid), urea and Ca2+. A strong fluorescence quenching effect was found when dithiothreitol and sodium dodecyl sulfate were added to the enzyme. TDSC showed antifungal activity against Aspergillus niger and Trichoderma sp. as tested by MTT assay and disc diffusion method. © 2015 Elsevier B.V.


PubMed | University of Rajshahi, Yokohama City University and Bangladesh Council of Scientific and Industrial Research Laboratories
Type: | Journal: International journal of biological macromolecules | Year: 2016

Chitinases are a group of enzymes that show differences in their molecular structure, substrate specificity, and catalytic mechanism and widely found in organisms like bacteria, yeasts, fungi, arthropods actinomycetes, plants and humans. A novel chitinase enzyme (designated as TDSC) was purified from Trichosanthes dioica seed with a molecular mass of 391 kDa in the presence and absence of -mercaptoethanol. The enzyme was a glycoprotein in nature containing 8% neutral sugar. The N-terminal sequence was determined to be EINGGGA which did not match with other proteins. Amino acid analysis performed by LC-MS revealed that the protein was rich in leucine. The enzyme was stable at a wide range of pH (5.0-11.0) and temperature (30-90 C). Chitinase activity was little bit inhibited in the presence of chelating agent EDTA (ethylenediaminetetraaceticacid), urea and Ca(2+). A strong fluorescence quenching effect was found when dithiothreitol and sodium dodecyl sulfate were added to the enzyme. TDSC showed antifungal activity against Aspergillus niger and Trichoderma sp. as tested by MTT assay and disc diffusion method.


Mohamud A.S.M.,Bangladesh Council of Scientific and Industrial Research Laboratories | Chowdhury M.,Shahjalal University of Science and Technology | Karim R.,Bangladesh Institute of Technology | Islam S.,Bangladesh Council of Scientific and Industrial Research Laboratories | And 5 more authors.
Journal of Pure and Applied Microbiology | Year: 2014

A total of five different Lactic acid bacterial species were identified from yoghurt samples collected from Chittagong region of Bangladesh. Among the isolates, the prevalence of Lactobacillus delbrueckii and Lactobacillus plantarum were 111 and 114cfu/ml respectively. After identification and isolation, we have optimized the suitable growth of isolates against pH, temperature and various concentration of NaCl. The results showed that better growth of organisms was observed in the presence of 3-6% NaCl. These species can tolerate up to 3% bile salt but the best tolerance found at 1% bile salt. To evaluate the antimicrobial properties of identified species, growth inhibition test has been done against some selected pathogens. It showed that Lactobacilli inhibited the growth of all pathogenic bacterial species. Inhibition by isolates and further treated with papain indicate that our isolates inhibited the growth of pathogenic microorganisms by producing Bacteriocins like substances. The selected species were subjected to Antibiotic Susceptibility testing to observe their features of carrying Antibiotic resistance genes. Among the 21 Antibiotics 17 antibiotics found resistant to Lactobacillus delbrueckii, on the other hand, Lactobacillus plantarum were found resistant to 4 antibiotics.


Kabir S.R.,University of Rajshahi | Nabi M.M.,University of Rajshahi | Nurujjaman M.,University of Rajshahi | Reza M.A.,University of Rajshahi | And 8 more authors.
Applied Biochemistry and Biotechnology | Year: 2015

In last three decades, several studies were carried out on the d-galactose-specific lectin of Momordica charantia seeds (MCL). In the present study, in vitro growth inhibition (8–23 %) at different concentrations (6–24 μg/ml) of MCL was observed against Ehrlich ascites carcinoma (EAC) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MCL also showed 28, 45, and 75 % growth inhibitions against EAC cells when administered 1.2, 2.0, and 2.8 mg/kg/day (i.p.), respectively for five consequent days in vivo in mice. After lectin treatment, the level of red blood cell and hemoglobin was increased significantly with the decrease of white blood cell and maintained the normal level when compared with EAC-bearing control and normal mice without EAC cells. Although MCL caused cell cycle arrest at G0/G1 phase of EAC cells, any irregular shape or apoptotic morphological alterations in the lectin-treated EAC cells was not observed by an optical and fluorescence microscope. Lectin showed toxicity against brine shrimp nauplii with an LC50 value of 49.7 μg/ml. Four out of seven pathogenic bacteria were agglutinated by MCL in the absence of inhibitory sugar d-lactose/d-galactose. In conclusion, MCL showed strong cytotoxic effect and therefore can be used as a potent anticancer chemotherapeutic agent. © 2014, Springer Science+Business Media New York.


Biswas S.K.,Bangladesh University | Das J.,Bangladesh University | Chowdhury A.,Bangladesh University | Karmakar U.K.,Khulna University | Hossain H.,Bangladesh Council of Scientific and Industrial Research Laboratories
Research Journal of Medicinal Plant | Year: 2012

Bacopa monniera commonly known as Brahmi grows in Bangladesh. The objective of the present study was to investigate the antinociceptive and antioxidant activities of ethanol extract of the whole plant of (B, monniera). The antinociceptive potential of the plant was determined using acetic acid induced writhing method. The results showed 39.79% (p<0.05) inhibition of writhings in the experimental animals when the plant extract was given intraperitoneally (i.p.) at 250 mg kg-1 b.wt. However, the extract produced maximum 56.14% (p<0.05) acetic acid induced writhing inhibition in mice at the dose of 500 mg kg-1 b.wt. This was also found to be comparable to the standard drug, Diclofenac-Na at 25 mg kg-1 b.wt. which inhibited 76.52% (p<0.05) of writhing reflex. The plant extract showed a dose dependent antinociceptive activity in acetic induced writhing model in mice. It was also found that the obtained p-values calculated by student's t-test were statistically significant. In order to determine the antioxidant activity of B. monniera, the extract was also assessed by l,l-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. The plant extract showed significant DPPH free radical scavenging effect compared to the standard antioxidants such as ascorbic acid and Butylated Hydroxy Anisole (BHA). The IC50 values of the extract, ascorbic acid and BHA were 79.84, 9.45 and 14.15 μg mL-1, respectively. Thus, the present study confirmed the antinociceptive and antioxidant activities of the ethanol extract of the whole plant of B. monniera. Finally, it is suggested to do further research for isolation and identification of the chemical structures of the phytochemical compounds responsible for antinociceptive and antioxidant activities of B. monniera. © 2012 Academic Journals Inc.


Kabir S.R.,University of Rajshahi | Rahman M.M.,University of Rajshahi | Amin R.,Bangladesh Council of Scientific and Industrial Research Laboratories | Karim M.R.,Bangladesh Council of Scientific and Industrial Research Laboratories | And 2 more authors.
Tumor Biology | Year: 2016

Recently, a lectin was purified from the potato cultivated in Bangladesh locally known as Sheel. In the present study cytotoxicity of the lectin against Ehrlich ascites carcinoma (EAC) cells was studied by MTT assay in vitro in RPMI-1640 medium and 8.0–36.0 % cell growth inhibition was observed at the range of 2.5–160 μg/ml protein concentration when incubated for 24 h. The lectin-induced apoptosis in EAC cells was confirmed by fluorescence and optical microscope. The apoptotic cell death was also confirmed by using caspase inhibitors. Cells growth inhibition caused by the lectin (36 %) was remarkably decreased to 7.6 and 22.3 % respectively in the presence of caspase-3 and -8 inhibitors. RT-PCR was used to evaluate the expression of apoptosis-related genes Bcl-X, p53, and Bax. An intensive expression of Bcl-X gene was observed in untreated control EAC cells with the disappeared of the gene in Sheel-treated EAC cells. At the same time, Bax gene expression appeared only in Sheel-treated EAC cells and the expression level of the p53 gene was increased remarkable after the treatment of EAC cells with the lectin. The lectin showed strong agglutination activity against EAC cells. Flow cytometry was used to study the cell cycle phases of EAC cells and it was observed that the lectin arrested the G2/M phase. In conclusion, Sheel lectin inhibited EAC cells growth by inducing apoptosis. © 2016 International Society of Oncology and BioMarkers (ISOBM)


PubMed | University of Rajshahi, Bangladesh Council of Scientific and Industrial Research Laboratories and Environmental Microbiology Laboratory
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2016

Recently, a lectin was purified from the potato cultivated in Bangladesh locally known as Sheel. In the present study cytotoxicity of the lectin against Ehrlich ascites carcinoma (EAC) cells was studied by MTT assay in vitro in RPMI-1640 medium and 8.0-36.0% cell growth inhibition was observed at the range of 2.5-160g/ml protein concentration when incubated for 24h. The lectin-induced apoptosis in EAC cells was confirmed by fluorescence and optical microscope. The apoptotic cell death was also confirmed by using caspase inhibitors. Cells growth inhibition caused by the lectin (36%) was remarkably decreased to 7.6 and 22.3% respectively in the presence of caspase-3 and -8 inhibitors. RT-PCR was used to evaluate the expression of apoptosis-related genes Bcl-X, p53, and Bax. An intensive expression of Bcl-X gene was observed in untreated control EAC cells with the disappeared of the gene in Sheel-treated EAC cells. At the same time, Bax gene expression appeared only in Sheel-treated EAC cells and the expression level of the p53 gene was increased remarkable after the treatment of EAC cells with the lectin. The lectin showed strong agglutination activity against EAC cells. Flow cytometry was used to study the cell cycle phases of EAC cells and it was observed that the lectin arrested the G2/M phase. In conclusion, Sheel lectin inhibited EAC cells growth by inducing apoptosis.

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