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Begum M.C.,University of Rajshahi | Islam M.S.,University of Rajshahi | Islam M.,University of Rajshahi | Amin R.,Bangladesh Council of Scientific and Industrial Research BCSIR Laboratories | And 2 more authors.
Plant Physiology and Biochemistry | Year: 2016

The arsenic (As) is a toxic element causing major health concern worldwide. Arsenate stress caused no significant reduction in growth parameters and shoot electrolyte leakage but showed increased root arsenate reductase activity along with relatively lower root As content and shoot translocation rate in As-tolerant BRRI 33 than in As-sensitive BRRI 51. It indicates that As inhibition and tolerance mechanisms are driven by root responses. Interestingly, As stress showed consistent decrease in phosphate content and expression of phosphate transporters (OsPT8, OsPT4, OsPHO1;2) under both high and low phosphate conditions in roots of BRRI 33, suggesting that limiting phosphate transport mainly mediated by OsPHO1;2 directs less As accumulation in BRRI 33. Further, BRRI 33 showed simultaneous increase in OsPCS1 (phytochelatin synthase) expression and phytochelatins (PCs) content in roots under As exposure supporting the hypothesis that root As sequestration acts as 'firewall system' in limiting As translocation in shoots. Furthermore, increased CAT, POD, SOD, GR, along with elevated glutathione, methionine, cysteine and proline suggests that strong antioxidant defense plays integral part to As tolerance in BRRI 33. Again, BRRI 33 self-grafts and plants having BRRI 33 rootstock combined with BRRI 51 scion had no adverse effect on morphological parameters but showed reduced As translocation rate, increased root arsenate reductase activity, shoot PC synthesis and root OsPHO1;2 expression due to As stress. It confirms that signal driving As tolerance mechanisms is generated in the roots. These findings can be implemented for As detoxification and As-free transgenic rice production for health safety. © 2016 Elsevier Masson SAS.

Hossain H.,Bangladesh Council of Scientific and Industrial Research BCSIR Laboratories | Karmakar U.K.,Khulna University | Biswas S.K.,Bangladesh University | Shahid-Ud-Daula A.F.M.,Noakhali Science and Technology University | And 3 more authors.
Pharmaceutical Biology | Year: 2013

Context: Ageratum conyzoides Linn. (Asteraceae) is an annual herbaceous plant with a long history of traditional medicinal and agricultural uses; it is usually grown in the northeast part of Bangladesh. Objective: The ethanol extract of the plant leaves was evaluated for preliminary phytochemical screening with its antinociceptive and antioxidant activities. Materials and methods: The preliminary phytochemical analysis was performed on the basis of standard procedures. The analgesic activity of the extract was investigated using the acetic acid-induced writhing method in mice. Five complementary tests such as DPPH free radical scavenging, nitric oxide (NO) scavenging, reducing power, Fe++ ion chelating ability and total phenolic content were used for determining antioxidant activities. Results: The results of preliminary phytochemical analysis showed the presence of alkaloids, reducing sugars, saponins, gums, steroids, tannins and flavonoids. The extract possessed a significant dose-dependent DPPH free radical scavenging activity with an IC50 value of 18.91 μg/ml compared to ascorbic acid (IC50: 2.937 μg/ml) and butylated hydroxyanisole (IC50: 5.10 μg/ml). The IC50 value of the extract for NO scavenging (41.81 μg/ml) was also found to be significant compared to the IC50 value of ascorbic acid (37.93 μg/ml). Moreover, the extract showed reducing power activity and Fe++ ion chelating ability. The total phenolic amount was also calculated as quite high (378.37 mg/g of gallic acid equivalents) in the crude ethanol extract. Discussion and conclusion: Therefore, the obtained results tend to suggest the antinociceptive and antioxidant activities of the ethanol extract of the plant leaves and justify its use in folkloric remedies. © 2013 Informa Healthcare USA, Inc.

Rahman M.Z.,University of Rajshahi | Rahman M.Z.,Bangladesh Council of Scientific and Industrial Research BCSIR Laboratories | Shahinul Islam S.M.,University of Rajshahi | Chowdhury A.N.,Bangladesh Council of Scientific and Industrial Research BCSIR Laboratories | Subramaniam S.,Universiti Sains Malaysia
Scientia Horticulturae | Year: 2015

A laboratory scale bioreactor system has been developed using nutrient spray technology for in vitro mass production of potato microtubers. Its effectiveness on the production of microtubers was investigated and compared with conventional liquid and semi-solid culture systems through bioreactor. Optimal culture conditions such as spray intervals, varying concentrations of 6-benzylaminopurine (BAP) and explants density were determined for the NSB. In order to determine optimal spray intervals, liquid medium was sprayed inside the NSB at different intervals (1/2, 1-4 h) of which the 1. h interval resulted in the highest number of shoots (3.47) and length (8.99 cm). Number of microtubers produced (5.13) was highest with 1. h intervals and fresh weight of microtubers (0.90 g) was highest for 1/2 h interval. Different concentrations of BAP (0.5, 1.0 and 1.5 mg/L) were used to evaluate its effect on microtuberization. It was observed that number and diameter of microtubers were increased (5.31 and 0.96 cm) when 0.5. mg/L BAP was supplemented in MS medium. We found fresh weight of microtubers (0.97. g) was increased when 1.0. mg/L BAP were added to the medium. In order to determine suitable explants density, single nodes grouped into five categories e.g 30, 45, 60, 75, and 90 and placed in the NSB system. A density of 60 explants resulted in increases in shoot length (17.5 cm) number of internodes (12.5) and with highest amount of chlorophyll (40.2. mg/g) as well as with highest number and fresh weight of microtubers (4.43 and 0.89 g, respectively). Out of the three culture systems, the NSB performed best where 1.5-2.0 fold increases in shoot growth and microtuberization without hyperhydration. The NSB also produced the highest number (4.67), fresh weight (0.86 g) and diameter (0.78 cm) of microtubers. From this study we may conclude that the NSB system has good potential for commercial mass production of potato micro-tuber. © 2015 Elsevier B.V.

Aziz S.,Chemical Research Division | Saha K.,Jahangirnagar University | Sultana N.,Bangladesh Council of Scientific and Industrial Research BCSIR Laboratories | Nur H.P.,Bangladesh Council of Scientific and Industrial Research BCSIR Laboratories | And 3 more authors.
Asian Pacific Journal of Tropical Biomedicine | Year: 2016

Objective: To investigate the elemental composition of the leaves and flowers of Catharanthus roseus (C. roseus) due to the plant's wide application in the indigenous medicinal system and its chemical constituents' importance. Methods: The atomic absorption spectrophotometer was used for quantitative analysis of various elements. Results: Total 13 important elements were analyzed in leaves and flowers of C. roseus. Results indicated the presence of Na, K, Ca, Mg, Cr, Fe, Zn, Al, Cu, Ni, Pb, Cd and Mn in both leaves and flowers. The most important finding of the work was that, leaves of C. roseus showed high concentration of all elements except K and Zn while flowers of C. roseus showed higher concentration of K and Zn. Conclusions: The elemental composition in both leaves and flowers of C. roseus were found to be different. Therefore, different parts of this medicinal plant are enriched in some micro and macro nutrients like Fe, Ca, Na, K, Zn, which are very important for biological metabolic system as well as human health. © 2015 Hainan Medical University.

Abu Bakar M.,Bangladesh Council of Scientific and Industrial Research BCSIR Laboratories | Islam S.,Bangladesh Council of Scientific and Industrial Research BCSIR Laboratories | Abdullah A.M.,Bangladesh Council of Scientific and Industrial Research BCSIR Laboratories
Electronic Journal of Environmental, Agricultural and Food Chemistry | Year: 2011

Potassium, Sodium, Calcium, Magnesium, Iron, Manganese, Copper, Chromium and Zinc contents were measured in fifteen types of vegetables which are available at Chittagong City in Bangladesh. Analyses were conducted at Phytochemistry and Pharmaceutical Chemistry Section in Bangladesh Council of Scientific and Industrial Research (BCSIR) Laboratories, Chittagong, Bangladesh using a sensitive analytical technique-AAS during January 2010 to December 2010. All materials contained high amounts of Mg, Na. Fe and K. The highest levels of Mg, Na, Fe and K were found in Long Bean (Barbati), Green banana (kach kala), Ladies finger (Dherosh), Pointed Gourd (Potol) respectively. Over all mean value of Magnesium, Sodium, Iron, Manganese, Copper, Chromium, Zinc and Potassium content of these items were 2.0 mg/100g, 0.79 mg/100g, 0.77 mg/100g, 0.164 mg/100g, 0.103 mg/100g, 0.0224 mg/100g, 0.27 mg/100g and 2.43mg/100g respectively. Mn, Cu and Cr contents in all vegetables were found to be very low. This work attempts to contribute to knowledge of the nutritional properties of these plants. These results may be useful for the evaluation of dietary information.

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