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Faiao-Flores F.,Butantan Institute | Faiao-Flores F.,University of Sao Paulo | Suarez J.A.Q.,Bandeirante University of Sao Paulo | Maria-Engler S.S.,University of Sao Paulo | And 3 more authors.
Tumor Biology | Year: 2013

The main difficulty in the successful treatment of metastatic melanoma is that this type of cancer is known to be resistant to chemotherapy. Chemotherapy remains the treatment of choice, and dacarbazine (DTIC) is the best standard treatment. The DM-1 compound is a curcumin analog that possesses several curcumin characteristics, such as antiproliferative, antitumor, and antimetastatic properties. The objective of this study was to evaluate the signaling pathways involved in melanoma cell death after treatment with DM-1 compared to the standard agent for melanoma treatment, DTIC. Cell death was evaluated by flow cytometry for annexin V and iodide propide, cleaved caspase 8, and TNF-R1 expression. Hoechst 33342 staining was evaluated by fluorescent microscopy; lipid peroxidation and cell viability (MTT) were evaluated by colorimetric assays. The antiproliferative effects of the drugs were evaluated by flow cytometry for cyclin D1 and Ki67 expression. Mice bearing B16F10 melanoma were treated with DTIC, DM-1, or both therapies. DM-1 induced significant apoptosis as indicated by the presence of cleaved caspase 8 and an increase in TNF-R1 expression in melanoma cells. Furthermore, DM-1 had antiproliferative effects in this the same cell line. DTIC caused cell death primarily by necrosis, and a smaller melanoma cell population underwent apoptosis. DTIC induced oxidative stress and several physiological changes in normal melanocytes, whereas DM-1 did not significantly affect the normal cells. DM-1 antitumor therapy in vivo showed tumor burden decrease with DM-1 monotherapy or in combination with DTIC, besides survival rate increase. Altogether, these data confirm DM-1 as a chemotherapeutic agent with effective tumor control properties and a lower incidence of side effects in normal cells compared to DTIC. © 2013 International Society of Oncology and BioMarkers (ISOBM). Source


Vila R.,University of Barcelona | Santana A.I.,University of Panama | Perez-Roses R.,University of Barcelona | Valderrama A.,Instituto Conmemorativo Gorgas En Estudio Of Salud | And 5 more authors.
Bioresource Technology | Year: 2010

The essential oil from fresh leaves of Plinia cerrocampanensis Barrie (Myrtaceae), obtained by hydrodistillation, was analysed by GC-FID and GC-MS. Forty components, representing more than 91% of the oil, were identified. Oxygenated sesquiterpenes represented the main fraction with α-bisabolol (42.8%) as the major constituent, making this plant a new and good source of this substance. Biological activity of the essential oil was evaluated against several bacterial and fungal strains as well as larvae from Aedes aegypti. The highest activity was found against Staphylococcus aureus, Pseudomonas aeruginosa, Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum with MIC values from 32 to 125 μg/ml. The essential oil also showed potent inhibitory and bactericidal activities against three H. pylori strains, with MIC and MBC values of 62.5 μg/ml, and caused 100% mortality of A. aegypti larvae at a concentration of 500 μg/ml. © 2009 Elsevier Ltd. All rights reserved. Source


Ereifej N.,University of Jordan | Ereifej N.,University of Manchester | Rodrigues F.P.,Bandeirante University of Sao Paulo | Silikas N.,University of Manchester | And 2 more authors.
Dental Materials | Year: 2011

Objectives: To compare the bond strength of a ceramic veneer material to two different ceramic core materials using shear strength testing and finite element analysis (FEA). Methods: 15 blocks of ceramic cores were made of each of IPS e.max ZirCAD (Ivoclar-Vivadent, Schaan, Liechtenstein) (ZirCAD) and lithium disilicate IPS e.max CAD (LS). These were veneered using IPS e.max Ceram (Ceram) according to the manufacturers' instructions. Samples were then mounted, placed inside a shear testing jig and loaded at their core/veneer interfaces with a universal testing machine. Fractured specimens were then examined fractographically at 45×. An independent t-test and a Mann-Whitney U test were used to detect differences in bond strengths and the patterns of failure between the two groups, respectively (p > 0.05). Two-dimensional plane stress FE-models were constructed and subjected to shear loading simulating the experimental conditions. Results: Mean shear stresses (MPa) were 28.8 (9.5) for ZirCAD/Ceram and 29.1 (8.3) for LS/Ceram. Differences were not statistically significant. Fracture patterns were significantly different between the 2 groups as all ZirCAD/Ceram samples broke adhesively at their interface while LS/Ceram samples broke cohesively in the veneer or the core or had mixed adhesive/cohesive failure. Stress distributions in the FEA models were also different, corresponding to the variable fracture patterns in the 2 groups. Significance: Although shear stresses were similar, fractographic analysis and finite element modeling suggested better bonding between the veneering ceramic and the glass-ceramic than to the zirconia cores. Improved bonding techniques are necessary to prevent clinical delamination of veneered zirconia restorations. © 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. Source


Pashley D.H.,Georgia Regents University | Tay F.R.,Georgia Regents University | Breschi L.,University of Trieste | Breschi L.,CNR Institute of Molecular Genetics | And 5 more authors.
Dental Materials | Year: 2011

Objectives: The aim of this study was to explore the therapeutic opportunities of each step of 3-step etch-and-rinse adhesives. Methods: Etch-and-rinse adhesive systems are the oldest of the multi-generation evolution of resin bonding systems. In the 3-step version, they involve acid-etching, priming and application of a separate adhesive. Each step can accomplish multiple goals. Acid-etching, using 32-37% phosphoric acid (pH 0.1-0.4) not only simultaneously etches enamel and dentin, but the low pH kills many residual bacteria. Results: Some etchants include anti-microbial compounds such as benzalkonium chloride that also inhibits matrix metalloproteinases (MMPs) in dentin. Primers are usually water and HEMA-rich solutions that ensure complete expansion of the collagen fibril meshwork and wet the collagen with hydrophilic monomers. However, water alone can re-expand dried dentin and can also serve as a vehicle for protease inhibitors or protein cross-linking agents that may increase the durability of resin-dentin bonds. In the future, ethanol or other water-free solvents may serve as dehydrating primers that may also contain antibacterial quaternary ammonium methacrylates to inhibit dentin MMPs and increase the durability of resin-dentin bonds. The complete evaporation of solvents is nearly impossible. Significance: Manufacturers may need to optimize solvent concentrations. Solvent-free adhesives can seal resin-dentin interfaces with hydrophobic resins that may also contain fluoride and antimicrobial compounds. Etch-and-rinse adhesives produce higher resin-dentin bonds that are more durable than most 1 and 2-step adhesives. Incorporation of protease inhibitors in etchants and/or cross-linking agents in primers may increase the durability of resin-dentin bonds. The therapeutic potential of etch-and-rinse adhesives has yet to be fully exploited. © 2010 Academy of Dental Materials. Source


Araujo G.S.A.,Piracicaba Dental School | Sfalcin R.A.,Piracicaba Dental School | Araujo T.G.F.,Piracicaba Dental School | Alonso R.C.B.,Bandeirante University of Sao Paulo | Puppin-Rontani R.M.,Piracicaba Dental School
Journal of Dentistry | Year: 2013

Objectives To evaluate the properties of experimental infiltrant blends by comparing them with the commercial infiltrant Icon® and penetration homogeneity into enamel caries lesions. Methods Groups were set up as follows: G1 (TEGDMA 100%); G2 (TEGDMA 80%, Ethanol 20%); G3 (TEGDMA 80%, HEMA 20%); G4 (TEGDMA 75%, BisEMA 25%); G5 (TEGDMA 60%, BisEMA 20%, Ethanol 20%); G6 (TEGDMA 60%, BisEMA 20%, HEMA 20%); G7 (TEGDMA 75%, UDMA 25%); G8 (TEGDMA 60%, UDMA 20%, Ethanol 20%); G9 (TEGDMA 60%, UDMA 20%, HEMA 20%) and Icon ®. Ten specimens were comprised by each group for the following tests (n = 10): degree of conversion (DC), elastic modulus (EM), Knoop hardness (KH), and softening ratio (SR). Infiltrant penetration was evaluated using confocal microscopy (CLSM). Data were subjected to two-way ANOVA and a Tukey's test (5%). Data comparing experimental materials and Icon® were analysed using ANOVA and Dunnett's test (5%). Results The highest DC values were found in G1, G7, G8, and G9. The lowest DC values were found in G2, G4, G5, and G6. EM and KHN were significantly lower in HEMA and with ethanol addition for all blends, except for G9. There was no significant difference among the groups regarding SR, and it was not possible to take KHN readings of G2, G5, and G8 after storage. There was no significant difference among groups for infiltrant penetration into enamel lesions. Conclusions The addition of hydrophobic monomers and solvents into TEGDMA blends affected DC, EM, and KHN. UDMA added to TEGDMA resulted in an increase in DC, EM, and KHN. Overall, solvents added to monomer blends resulted in decreased properties. The addition of hydrophobic monomers and solvents into TEGDMA blends does not improve the penetration depth of the infiltrants. © 2013 Elsevier Ltd. All rights reserved. Source

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