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Cristina R.T.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Obistioiu D.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Dumitrescu E.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Nichita I.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | And 3 more authors.
Turkish Journal of Biology | Year: 2015

From our prior studies concerning eugenol’s biologic activity we observed the very good in vitro antifungal efficiency of this natural compound. Those positive results generated the need to supplement the available information with a comparative in vivo animal model. In this context, our current study was proposed to ascertain and compare the effects of eugenol with nystatin with a placebo control group (saline solution) by evaluating the cytohistological alterations in immunosuppressed Wistar female rats genitally infected with Candida albicans. The observed histological alterations were most apparent in the placebo group. Candidiasis affected the liver, ovaries, kidneys, and spleen of the infected female rats. When compared to the groups treated with eugenol and nystatin, these alterations were either less discernible or were not reported at all, except for the liver degenerations, which were attributed to the immunosuppressive activity of drugs used and not to the Candida infection. The efficiency in the in vivo experimental candidiasis exerted by eugenol upon the tissues’ histoarchitecture allows us to seriously consider it as a promising antifungal active substance, useful therapeutically in genital candidiasis and, in our opinion, with comparable biologic activity to that of nystatin. © TÜBİTAK. Source


Dumitrescu E.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Chiurciu V.,Romvac Company | Muselin F.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Popescu R.,Victor Babes University of Medicine and Pharmacy Timisoara | And 2 more authors.
Turkish Journal of Biology | Year: 2015

The aim of the current study was to evaluate lead accumulation in the ovaries, fallopian tubes, and uterus and to take note of any consequent histo-architectural changes. The experiment involved a 12-month chronic exposure of 28 Wistar female rats at sexual maturity (221 ± 0.88 g/individual) to lead acetate in drinking water. The rats were divided into 4 groups based on the level of lead exposure: E1 at 0.050 mg L–1, E2 at 0.100 mg L–1, E3 at 0.150 mg L–1 , and a control group that received tap water. Lead level evaluation was performed by atomic absorption spectrometry at 283.3 nm and the histo-architectonics in target organs were evaluated after hematoxylin and eosin staining and microscopy. The exposure to lead acetate produced significant histological alterations caused by lead accumulation in the sexual organs. These structural changes correlated with the level of exposure in the ovaries, uterus, and fallopian tubes. They were mainly edemas and necrosis, denudation, and/or different stages of follicle evolution. These alterations have been shown to indicate infertility in female rats. © TÜBİTAK. Source


Mosneang C.L.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Dumitrescu E.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Muselin F.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Ciulan V.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | And 2 more authors.
Polish Journal of Environmental Studies | Year: 2015

Our study used mortality and developmental abnormalities present in fertilized zebra fish eggs as a possible useful bio-indicator to assess water pollution from the vicinity of five great swine units using 48-hour in vitro zebra fish eggs acute toxicity test. In assay, 1,300 (Test) and 220 (Control) healthy fertilized zebra fish eggs were used. During the test duration, a number of dead eggs or embryos and/or any other disturbance in the embryonic development that could indicate pollution were ascertained. In parallel, embryonic mortality was assessed for each swine unit possible farm effluent source, using double samples and 10 different water dilutions provided from the environs river. It was found in some situations that fertilized fish egg development was disturbed by the water components at the assay moment. Statistical results, analyzed according to the Kolmogorov-Smirnov normality test (significant P<0.05, or lower), revealed that, from the five observed units at the sample collection moment, the river water was polluted in the surroundings of swine Units 1 and 4, which was also confirmed by the acute toxicity endpoints presence in developed embryos in the water samples provided. This qualitative assay could be a helping tool for small-scale environmental risk assessment due to the opportunity to achieve, in a short amount of time, an accurate analysis. © 2015, Polish Journal of Environmental Studies. All rights reserved. Source


Muselin F.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Cristina R.T.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Igna V.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | Dumitrescu E.,Banats University Of Agriculture And Veterinary Medicine King Michael Romania | And 2 more authors.
Turkish Journal of Medical Sciences | Year: 2016

Background/aim: The effects of aluminium exposure on reproductive biomarkers in male rats were followed in a three-generation study. Materials and methods: Forty Wistar male rats (F0) were divided into the following groups: control (C), receiving only tap water, and three experimental (E) groups, receiving aluminium sulphate (AS) (E1: 200 ppb, E2: 400 ppb, and E3: 1000 ppb) in drinking water for a 6-month exposure period. To obtain F1, three males from each group were mated with previously unexposed females (1:2 sex ratios) that during gestation and lactation were exposed to the same AS levels as males. The F1 generation male offspring were divided as described and exposed to the same AS levels. The protocol to obtain F2 was similar to that described for FResults: Significantly lower testosterone levels in rats exposed to AS and in generations F1 and F2 compared to the parental one, luteinising hormone (LH) fluctuations in F0 and a significant LH decrease in F2 and F3 generations, testis weight decrease, increased immobile and abnormal sperm, and histoarchitecture alterations in the testes were observed. Moreover, interval between deliveries increased. Conclusion: Chronic exposure to AS was significantly deleterious, producing a pronounced decrease in the sperm count and testosterone levels in all experimental groups. © TÜBİTAK. Source

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