Time filter

Source Type

Cerasela P.,Banats University of Agricultural Sciencies and Veterinary Medicine | Simona L.,Banats University of Agricultural Sciencies and Veterinary Medicine | Giancarla V.,Banats University of Agricultural Sciencies and Veterinary Medicine | Marcel D.,Banats University of Agricultural Sciencies and Veterinary Medicine | And 3 more authors.
Romanian Biotechnological Letters | Year: 2014

The objective of this study was the improvement of in vitro plant regeneration of few German iris varieties (Blue Bird Wine, Tangerine Charm, Glazed Orange, Jane Philips) via somatic embryogenesis using leaf base. For callus and embryogenesis induction MS medium added with dichlorophenoxyacetic acid, α-nafthyl acetic acid, and kinetin was used. The frequency of explants with regeneration ability was up to 40% in all four varieties selected for this study. The number of regenerants per explant varied between 0 and 8. For micropropagation, the most crucial aspect is to maintain the genetic conformity, the regenerants have to be identical to the mother plant. For the assessment of in vitro stability/instability of the regenerated plants, we used RAPD molecular markers. For DNA extraction young leaves from 10 in vitro growing plants were sampled. The 10 RAPD primers were tested for detection of the genetic polymorphism among regenerated and mother plants. Only 5 produced reproducible fragments. RAPD analysis did not reveal any type of polymorphism between randomly selected in vitro plants and the mother plant, indicating the clonal nature of progenies. Our results showed an advantage of this in vitro propagation protocol because the risk of somaclonal variation is reduced.

Loading Banats University of Agricultural Sciencies and Veterinary Medicine collaborators
Loading Banats University of Agricultural Sciencies and Veterinary Medicine collaborators