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Hadaruga D.I.,Polytechnic University of Timişoara | Hadaruga D.I.,Clinical County Hospital of Timisoara | Birau C.L.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara | Gruia A.T.,Clinical County Hospital of Timisoara | And 4 more authors.
Food Chemistry | Year: 2017

The moisture content of β-cyclodextrin/Danube fish oils complexes (common barbel, Pontic shad, European wels catfish, common bleak) was evaluated by thermal methods. Saturated and monounsaturated fatty acids were the most concentrated in fish oils (25.3-30.8% and 36.1-45.0%). ω-3 And ω-6 fatty acids were identified in low concentrations of 2.8-12.1% and 4.1-7.1%. The moisture content was significantly lowered after β-CD complexation, as revealed by thermogravimetric (TG) analysis (13.3% for β-CD, 2.5-6.5% for complexes). These results are consistent with the differential scanning calorimetry (DSC) data for the peaks corresponding to dissociation of water (calorimetric effect of 536Jg-1 for β-cyclodextrin and 304-422.5Jg-1 for complexes). Furthermore, both TG and DSC results support the formation of inclusion complexes. This is the first study on the nanoencapsulation of Danube fish oils in β-cyclodextrin. © 2017.


PubMed | Sudan University of Science and Technology, University of Veterinary and Animal Sciences, Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara, Victor Babes University of Medicine and Pharmacy Timisoara and West University of Timișoara
Type: Journal Article | Journal: PloS one | Year: 2015

Land snails are highly tolerant to cadmium exposure and are able to accumulate soil cadmium independently of food ingestion. However, little information exists on the kinetics of cadmium retention in terrestrial gastropods exposed to an increase in the soil cadmium content, over time. There is also little knowledge about how exposure to cadmium-polluted soils influences shell growth and architecture. In this context, we examined cadmium accumulation in the hepatopancreas and shell of juvenile Cantareus aspersus exposed to elevating high levels of cadmium in soil. Also, the toxicity of cadmium to snails was assessed using a range of conchological endpoints, including shell height, width, volume, allometry and integrity. Test snails, aged three months, were reared under semi-field conditions, fed an uncontaminated diet and exposed first, for a period of 30 days, to a series of soil cadmium concentrations, and then, for a second period of 30 days, to soils with higher cadmium content. Cadmium showed a dose-dependent accumulation in both the hepatopancreas and shell. The kinetics of cadmium retention in the hepatopancreas of snails previously exposed to cadmium-spiked soils was significantly influenced by a new exposure event. The shell was not a relevant bioaccumulator for soil cadmium. Under the present experimental conditions, only high cadmium exposure significantly affected either the shell growth or snail survival. There was no consistent effect on shell allometry, but the shell integrity, especially in rapidly growing parts, appeared to be affected by high cadmium exposure. Our results attest to the value of hepatopancreas for describing cadmium retention in land snails and to the difficulty of using conchological parameters in field surveys for estimating the environmental hazard of soil cadmium.


PubMed | University of Veterinary and Animal Sciences and Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara
Type: Case Reports | Journal: Veterinary clinical pathology | Year: 2015

This case study describes the first genetically confirmed and clinically manifested autochthonous Ehrlichia canis infection in a 9-year-old female mixed-breed dog from Romania. Health screening of the dog included clinical examination, evaluation of stained peripheral blood smear and hematologic variables, as well as serologic testing and molecular analysis. Clinical signs included fever, apathy, dehydration, pale mucous membranes, and weakness. The microscopic examination of the blood smear and immunologic assays for Borrelia burgdorferi, Anaplasma phagocytophilum, and Ecanis antibodies, and for Dirofilaria immitis antigen yielded negative results. Hematologic abnormalities included moderate nonregenerative anemia, leucopenia with neutropenia, and moderate thrombocytopenia. The biochemical abnormalities identified were hypoalbuminemia, and mildly increased serum enzyme activities of AST and ALT. In addition, increased urea and creatinine levels associated with low urine specific gravity and proteinuria were also present. Nested PCR amplification of the partial Ecanis 16S rRNA gene demonstrated the presence of this rickettsial pathogen in the dogs blood, which subsequently was confirmed through sequencing based on the 100% homology with GenBank deposited Ecanis isolates. After specific treatment with doxycycline (10mg/kg, orally, SID) for onemonth, the proteinuria, and hematologic and serum biochemical abnormalities with the exception of mild azotemia resolved. This report supports the geographical expansion of canine ehrlichiosis caused by Ecanis in nonendemic regions of Europe.


PubMed | Polytechnic University of Timişoara and Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara
Type: Journal Article | Journal: International journal of environmental research and public health | Year: 2015

The effects of the sorption of environmental applications by various source materials of natural organic matter, i.e., bone powder, was examined. Sorption capacities and subsequent rare earth element retention characteristics of all metals tested were markedly increased by ionic task-specific. In this study, the abilities of three models isotherms widely were used for the equilibrium sorption data: Langmuir, Freundlich and Redlich-Peterson. For all studied metal ions the maximum adsorption capacity is close to those experimentally determined. The characteristic parameters for each isotherm and related coefficients of determination have been determined. The experimental data achieved excellent fits within the following isotherms in the order: Langmuir > Redlich-Peterson > Freundlich, based on their coefficient of determination values. The bone powder has developed higher adsorption performance in the removal process of Nd(III), Eu(III), La(III) from aqueous solutions than in the case of the removal process of Cs(I), Sr(II) and Tl(I) from aqueous solutions. The described relationships provide direct experimental evidence that the sorption-desorption properties of bone powder are closely related to their degree of the type of the metal. The results suggest a potential for obtaining efficient and cost-effective engineered natural organic sorbents for environmental applications.


PubMed | Sudan University of Science and Technology, Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara and West University of Timișoara
Type: Journal Article | Journal: PloS one | Year: 2015

The coffee oil has a promising potential to be used in food industry, but an efficient use, especially in products that required high-temperature heating, depends on its chemical composition and the changes induced by processing. Since there is little information on this topic, the aim of our study was to investigate the crude green and roasted coffee oil (GCO, RCO) and heated (HGCO, HRCO) for 1 h at 200C, by Fourier Transform Infrared (FTIR) spectroscopy and in terms of antioxidant and antimicrobial properties. The results of FTIR spectroscopy revealed that no statistically significant differences (one-way ANOVA, p>0.05) in the oxidative status of GCO and RCO were found. The coffee oils heating induced significant spectral changes in the regions 3100-3600 cm(-1), 2800-3050 cm(-1) and 1680-1780 cm(-1) proved by the differences in the absorbance ratios A 3009 cm(-1)/A 2922 cm(-1), A 3009 cm(-1)/A 2853 cm(-1), A 3009 cm(-1)/A 1744 cm(-1), A 1744 cm(-1)/A 2922 cm(-1). These alterations were related to the reduction of the unsaturation degree due to primary and secondary oxidation processes of the lipid fraction. The radical scavenging ability of oils investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay revealed that the IC50 value of GCO was significantly lower than of RCO (p<0.05). The IC50 values of crude coffee oils were lower than those of heated samples. The antioxidant activity of oils was attributed to both antioxidant compounds with free-radical scavenging capacity and to lipids oxidation products generated by heating. In the first 6 h of incubation, the inhibitory activity of crude oils against E. coli and E. faecalis was not significantly different to the control (p>0.05). Also, HGCO and HRCO showed significantly different inhibitory potential related to the control (p<0.05). The heating induced statistically significant decreases in the effectiveness of coffee oils against the tested bacteria. GCO proved to be the most effective among investigated coffee oils against the tested bacteria.


PubMed | Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara and University of Milan
Type: | Journal: Veterinary parasitology | Year: 2016

Forty seven working horses from Romania were post-mortem examined for small strongyles (Cyathostominae) infections. All horses were found infected. The overall cyathostomins intensity ranged from 390 to 13,010 and horses were infected by 8-24 species. The intensity was higher in ventral colon (1531) and dorsal colon (824), the lowest in the caecum (524). Twenty four species were identified. Cyathostomum catinatum, Cylicocyclus insigne, and C. Nassatus had 100% of prevalence. Over 50% of horses were infected by Coronocyclus coronatus, Cylicostephanus calicatus, C. goldi, and C. longibursatus. Other prevalent species (34%-45%) were Cyathostomum tetracanthum, Cylicostephanus minutus and Gyalocephalus capitatus. Coronocyclus labiatus, Parapoteriostomum mettami, Poteriostomum imparidentatum and P. ratzii had the lowest prevalence. Most species showed high organ preference with a niche breadth value between 1 and 1.96 while only 7 species (Coronocyclus labiatus, Cyathostomum tetracanthum, C. brevicapsulatus, Cylicocyclus elongatus, C. insigne, C. leptostomum and C. radiatus) showed a more generalist selection. The niche breadth of 10 species was significantly (p<0.05) influenced by itself intensity (Coronocyclus labratus, Cyathostomum pateratum, C. tetracanthum, Cylicocyclus elongatus, C. radiatus, C. ultrajectinus, C. leptostomum, Cylicodontophorus euproctus, Poteriostomum imparidentatum, P. ratzii). The niche breadth of Cylicocyclus nassatus was positively (p<0.05) influenced by the summed intensity of the other species while that of Cylicocyclus elongatus was negatively (p<0.05) influenced by the intensity of the other species. The cluster analysis of the Cyathostominae community composition showed a major cluster composed by the three dominant species, followed by a cluster composed by Coronocyclus coronatus, while all the other species presented a tree like structure.


Iovan M.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara | Radu F.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara | Rotaru L.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara
Revista de Chimie | Year: 2016

The nutritional quality of proteins in blends of amaranth-rice and quinoa-rice flours, has been determined by amino acid assay and by AOAC Official Method protein pattern. 100% whole rice flour was used as control. Afterwards, the whole rice flour was replaced by 10, 20 and 30% amaranth and quinoa flour, respectively, in order to prepare two sorts of gluten free sweet products (cookies and muffins). The effect of cooking (at 180oC for 20 min) on proteins and amino acid compositions of gluten-free cookies and muffins was studied, also. The amino acid composition of the protein in both flour blends of amaranth and quinoa show similar patterns. The first limiting amino acids were the aromatic amino acids tyrosine + phenylalanine, giving a chemical score of 86 for protein in 30% quinoa flour blends and 85 for protein in amaranth blends, respectively. Threonine was the next limiting amino acid followed by lysine. Based on different solubility in water, saline, and buffer, flour blends proteins were fractionated on as albumin-1 (Albu-1), albumin-2 (Albu-2), globulin (Glob), and glutelin (Glu) and were identified by AOAC Official Method protein pattern. Albu-1 was high in lysine (3.4-6.6 g 100 g-1 protein), while Albu-2, had a high leucine content (4.2-6.9 g 100 g - 1 protein). Glu fractions were well-balanced in their essential amino acids with the exception of methionine. The levels of tyrosine, threonine and tryptophan were in several cases negatively affected by the cooking procedure.


Moldovan C.,Banats University of Agricultural Science and Veterinary Medicine King Michael I of Romania from Timisoara | Druga M.,Banats University of Agricultural Science and Veterinary Medicine King Michael I of Romania from Timisoara | Dumbrava D.,Banats University of Agricultural Science and Veterinary Medicine King Michael I of Romania from Timisoara | Raba D.,Banats University of Agricultural Science and Veterinary Medicine King Michael I of Romania from Timisoara | And 4 more authors.
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM | Year: 2014

In this paper we have studied the influence of fruits adding in honey related to ascorbic acid and total polyphenols content. To determine the ascorbic acid it was used a spectrophotometric method with leuco-malachite-green. In order to determine the total polyphenols level there was used the Folin-Ciocalteu assay. The ascorbic acid content was improved in honey samples with fruits, varying between 3.27 and 4.01 mg/100 g while the control sample (honey without any fruit) registered 3.24 mg/100 g. The highest polyphenols level was found in honey fortified with raspberry (0.079 mg gallic acid equivalent /g) while the lowest (0.012 mg gallic acid equivalent /g) level of polyphenols was registered in the control honey samples. © SGEM2014.


PubMed | Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania from Timisoara
Type: Journal Article | Journal: The Journal of parasitology | Year: 2015

Blood samples of 119 red foxes, originating from 44 hunting grounds of 3 western counties (Arad, Hunedoara, and Timi) of Romania, have been examined for the presence of Hepatozoon canis infection using the conventional polymerase chain reaction (PCR) of the fragment of 18S rRNA gene. Overall, 15 (12.6%) samples were found to be PCR-positive. Of the sampled hunting grounds, 29.5% (13/44) were found positive. Positive samples were recorded in all screened counties with the prevalence of 14.8% (9/61) in Arad, 9.8% (5/51) in Timi, and 14.3% (1/7) in Hunedoara, respectively. No correlation was found (P > 0.05) between H. canis positivity and gender or territorial distribution of the infection. To confirm PCR results, 9 randomly selected amplicons were sequenced. The obtained sequences were identical to each other, confirmed the results of the conventional PCR, and showed 98-100% homology to other H. canis sequences. The results of the current survey support the role of red foxes as sylvatic reservoirs of H. canis in Romania.

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