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Raba D.N.,Sudan University of Science and Technology | Poiana M.-A.,Sudan University of Science and Technology | Borozan A.B.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania | Stef M.,West University of Timisoara | And 2 more authors.
PLoS ONE | Year: 2015

The coffee oil has a promising potential to be used in food industry, but an efficient use, especially in products that required high-temperature heating, depends on its chemical composition and the changes induced by processing. Since there is little information on this topic, the aim of our study was to investigate the crude green and roasted coffee oil (GCO, RCO) and heated (HGCO, HRCO) for 1 h at 200°C, by Fourier Transform Infrared (FTIR) spectroscopy and in terms of antioxidant and antimicrobial properties. The results of FTIR spectroscopy revealed that no statistically significant differences (one-way ANOVA, p>0.05) in the oxidative status of GCO and RCO were found. The coffee oils heating induced significant spectral changes in the regions 3100-3600 cm-1, 2800-3050 cm-1 and 1680-1780 cm-1 proved by the differences in the absorbance ratios A 3009 cm-1/A 2922 cm-1, A 3009 cm-1/A 2853 cm-1, A 3009 cm-1/A 1744 cm-1, A 1744 cm-1/A 2922 cm-1. These alterations were related to the reduction of the unsaturation degree due to primary and secondary oxidation processes of the lipid fraction. The radical scavenging ability of oils investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay revealed that the IC50 value of GCO was significantly lower than of RCO (p<0.05). The IC50 values of crude coffee oils were lower than those of heated samples. The antioxidant activity of oils was attributed to both antioxidant compounds with free-radical scavenging capacity and to lipids oxidation products generated by heating. In the first 6 h of incubation, the inhibitory activity of crude oils against E. coli and E. faecalis was not significantly different to the control (p>0.05). Also, HGCO and HRCO showed significantly different inhibitory potential related to the control (p<0.05). The heating induced statistically significant decreases in the effectiveness of coffee oils against the tested bacteria. GCO proved to be the most effective among investigated coffee oils against the tested bacteria. © 2015 Raba et al. Source


Rujescu C.I.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania | Ciolac M.R.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania | Martin S.C.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania | Butur M.,Victor Babes University of Medicine and Pharmacy Timisoara
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM | Year: 2014

The capitalization of agricultural production often takes place under price instability of final products. This provides the quantitative need to modify the resources allocated to the agricultural production process in order to obtain best economic values. More exactly, in a retroactive way, the economic optimum point will have different values for different times, corresponding to the variable price of capitalization of production. Specifically, this study presents an analysis of variance of necessary amount of nitrogen which must be optimally allocated according to a situation of instability in the price of wheat, for the interval of wheat price between 150 and 300 Euro/tonne. Furthermore, several remarks are made on the size of differences obtained. Different values obtained were characterized by differences of up to 9,7% on mentioned interval; they have significant implications for economic considerations; more important, the differences can also be approached from environmental reasons, regarding the negative impact of allocation of unjustified high amounts of chemicals. © SGEM2014. Source


Imre M.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania | Dudu A.,University of Bucharest | Ilie M.S.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania | Morariu S.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania | And 2 more authors.
Journal of Parasitology | Year: 2015

Blood samples of 119 red foxes, originating from 44 hunting grounds of 3 western counties (Arad, Hunedoara, and Timiş) of Romania, have been examined for the presence of Hepatozoon canis infection using the conventional polymerase chain reaction (PCR) of the fragment of 18S rRNA gene. Overall, 15 (12.6%) samples were found to be PCR-positive. Of the sampled hunting grounds, 29.5% (13/44) were found positive. Positive samples were recorded in all screened counties with the prevalence of 14.8% (9/61) in Arad, 9.8% (5/51) in Timiş, and 14.3% (1/7) in Hunedoara, respectively. No correlation was found (P > 0.05) between H. canis positivity and gender or territorial distribution of the infection. To confirm PCR results, 9 randomly selected amplicons were sequenced. The obtained sequences were identical to each other, confirmed the results of the conventional PCR, and showed 98-100% homology to other H. canis sequences. The results of the current survey support the role of red foxes as sylvatic reservoirs of H. canis in Romania. © 2015 American Society of Parasitologists. Source

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