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Iuliana C.,University of Veterinary and Animal Sciences | Cerasela P.,Banats University of Agricultural science and Veterinary Medicine
Romanian Biotechnological Letters | Year: 2014

Among the non-ionizing radiations, the ultraviolet radiations (UV) are the only ones which can change the genetic system. The investigations related to the soma clonal variability for potato (Solanum tuberosum) were focused on the response to the radiation exposure UV-C (λ = 254nm). The biological material used was represented by three types of potato: Cristian, Roclas and Rustic. For this purpose we used several RAPD markers (Random Amplified Polymorphic DNA). After the irradiation with UV-C there were some changes at molecular level by the occurrence in some cases of new DNA bands or the disappearance of DNA bands in other cases highlighted using three primers (OPF-12, OPW-11 and OPX-01). © 2014 University of Bucharest.

Hadaruga N.G.,Banats University of Agricultural science and Veterinary Medicine | Hadaruga D.I.,Polytechnic University of Mozambique | Isengard H.-D.,University of Hohenheim
Food Chemistry | Year: 2012

The paper presents a comparative study regarding the water determination in natural cyclodextrins and in their essential oil complexes (Apiaceae, Liliaceae, and Cupressaceae families) by using Karl Fischer titration (KFT) and thermal methods. For the natural cyclodextrins, the influence of the solvent hydrophobicity and the preheating temperature on the water extraction process were evaluated. The water contents, estimated by KFT in both methanol and methanol-octanol solvent systems, were 10.6% and 14.4% for α- and β-cyclodextrin, respectively; the water content, estimated by KFT in a more hydrophilic solvent system, methanol-formamide, was 0.4-0.6% higher. Thermogravimetric evaluation of water conducts to lower values. For the essential oil/cyclodextrin complexes, the KFT water content were in the range of 6.4-8.1%, higher values being obtained in the case of Juniperus essential oil/β-cyclodextrin complexes (7.5-8.1%). With some exceptions, thermal analyses of complexes are in good agreement with the KFT results. © 2011 Elsevier Ltd. All rights reserved.

Butu M.,Romanian National Institute for Research and Development for Biological Sciences | Rodino S.,Romanian National Institute for Research and Development for Biological Sciences | Rodino S.,University of Bucharest | Butu A.,Romanian National Institute for Research and Development for Biological Sciences | Butnariu M.,Banats University of Agricultural science and Veterinary Medicine
Digest Journal of Nanomaterials and Biostructures | Year: 2014

In the phyto-chemical research of extracts, there is a rising demand for simple, quick, selective and precise quantitative analysis methods of polyphenolics. The goal of this research was to realise a quantitative meta-analysis of the experimental methods that investigate flavonoids from three types of seeds of Lens culinaris Medikus extracts using spectrophotometry. Polyphenol quantitative determination was done through spectrophotometric methods (based on the chelating reaction with AlCl3/CH3COONa for flavones, and Arnow reaction for poliphenolcarboxylic acids). Determination of the antioxidant activity of the extract was realised with the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method and ORAC (antioxidative capacity of the oxygen radical) method. The antioxidant capacity of the analysed extracts is proportional with their concentration value. In order to use as nutraceuticals, the organic solvents were replaced with alimentary ethanol of fermentative origin, and HCl was replaced with acetic acid of 0.5% concentration.

Hadaruga D.I.,Polytechnic University of Timişoara | Hadaruga N.G.,Sudan University of Science and Technology | Butnaru G.,Banats University of Agricultural science and Veterinary Medicine | Tatu C.,County Hospital Timisoara | Gruia A.,County Hospital Timisoara
Journal of Inclusion Phenomena and Macrocyclic Chemistry | Year: 2010

The paper presents a comparative thermal and oxidative stability study between nicotine/β-cyclodextrin microparticles and commercial nicotine. It is well known that the nicotine is the bioactive compound in formulations used for smoking cessation and no studies among the stability of nicotine in cyclodextrin-containing formulations were reported. The non-enzymatic and enzymatic oxidation of nicotine can lead to cotinine (an alkaloid/metabolite with a lower toxicity), but another way is the obtaining of the cancerigene N-nitroso-nicotine derivatives by nornicotine derivative intermediates (like nornicotine and myosmine). The present study demonstrates the protecting capacity of β-cyclodextrin for commercial nicotine against thermal and oxidative factors: for the non-complexed nicotine the thermal and oxidative degradation led to a decrease of the relative concentration of nicotine from 96 to 92% for an increasing temperature from 30 to 90 °C (in the presence of air at normal pressure), with an increase of the relative concentration of the corresponding oxidized compounds (like cotinine and furthermore myosmine up to 0.7%, and up to 4. 7%, respectively). For the nicotine/β-cyclodextrin complex the interaction selectivity was higher for nicotine and the stability of this bioactive compound against oxidation was also higher in comparison with the non-complexed nicotine (around 98% in all cases). © 2010 Springer Science+Business Media B.V.

Micu L.M.,Banats University of Agricultural science and Veterinary Medicine | Petanec D.I.,Banats University of Agricultural science and Veterinary Medicine | Iosub-Ciur M.D.,Grigore T. Popa University of Medicine and Pharmacy | Andrian S.,Grigore T. Popa University of Medicine and Pharmacy | And 2 more authors.
Revista de Chimie | Year: 2016

In almost all areas of the world where there is a mining practice, this activity has led in time to a formation of tailings/sterile dumps, as a result of copper ore preparation by flotation. Prolonged exposure of plants to high concentrations of heavy metals within the sterile determine their accumulation in quantities that can affect plant metabolism and by trophic transfer, it will be a potential danger to the consumers" health. The purpose of this research was to determine the influence of tailings on bioaccumulation of potentially toxic metals in leaves of products of an eco and health importance (sea-buckthorn and blackberry), and econometric modeling data. For this purpose it was useful to analyze the medium level, the variant, and the homogenization degree of the data. The need for these studies resulted as a fact that sea-buckthorn and blackberry leaves are harvested by locals and used in popular medicine for rheumatism, stomach and skin diseases. The heavy metals (Fe, Mn, Zn, Cu, Ni, Cd, Pb) of the sea buckthorn and of the blackberry leaves were determined by atomic absorption spectrophotometry method. The statistical data taken were performed by using econometric software EViews 7.0. Our research reveals that mineralization of heavy metals in leaves is much higher than in fruit and a slow accumulation and during a period of time of these substances will negatively influence their quality and the consumers" metabolism. In the case of fruit shrubs (sea-buckthorn and blackberry) cropped/cultivated on copper dumps mining, potentially toxic metals bioaccumulate in much larger quantities in the leaves of blackberry, in comparison to those of the seabuckthorn" s, so the recommendation is that these leaves were not used for medical purposes.

Alexa E.,Sudan University of Science and Technology | Poiana M.-A.,Sudan University of Science and Technology | Sumalan R.-M.,Banats University of Agricultural science and Veterinary Medicine
International Journal of Molecular Sciences | Year: 2012

The aim of this study was to evaluate the effect of some natural extracts obtained from grape pomace (GPE) and grape seeds (GSE) as compared to a synthetic food, antioxidant-butylated hydroxytoluene (BHT), in order to control fungal population and ochratoxin A (OTA) production in naturally contaminated wheat. The results showed that the addition of these extracts was efficient with OTA content decreasing. On treatment with these extracts the loss of OTA content after 14 days was in the range 7.8-28.3% relative to the control sample, but increased up to 26.48-37% after 28 days. The highest loss in OTA content was recorded for treatment with GPE at the 500 ppm level. Regarding the fungal development, the obtained results show that the total fungal populations were significantly reduced by using natural extracts. The most efficient extract was GPE. Both BHT and GPE inhibit the growth of Penicillium verrrucosum, for all doses, but less with Aspergillus genera. GPE affects the growth of other types of moulds such as Rhizopus microsporus, Fusarium graminearum, Alternaria infectoria and Cladosporium herbarum. Our data suggested that GPE and GSE are able to provide fungicidal and fungistatic protection and to control the OTA accumulation in wheat, at least in a similar manner to BHT. © 2012 by the authors.

Jianu C.,Sudan University of Science and Technology | Pop G.,Banats University of Agricultural science and Veterinary Medicine | Gruia A.T.,County Hospital Timisoara | Horhat F.G.,Victor Babes University of Medicine and Pharmacy Timisoara
International Journal of Agriculture and Biology | Year: 2013

The purpose of this study was to determine the chemical composition and antimicrobial properties of essential oils (EOs) isolated from lavender (L. angustifolia Miller) and lavandin (Lavandula x intermedia) harvested in 2011 in western Romania. The essential oils, isolated by steam distillation from inflorescences arrived at full flowering stage, were analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The essential oil of L. angustifolia Miller analyzed contained as main components caryophyllene (24.1%), beta-phellandrene (16%) and eucalyptol (15.6%), while the essential oil of Lavandula x intermedia contains camphor (32.7%) and eucalyptol (26.9%). The antimicrobial activity was evaluated by the Kirby-Bauer method. Antimicrobial tests showed antimicrobial activity against Shigella flexneri, Staphylococcus aureus, E. coli and Salmonella typhimurium, while Streptococcus pyogenes is not sensitive to the action of the two essential oils. The study revealed that essential oils isolated and analyzed from lavender (L. angustifolia Miller) and lavandin (Lavandula x intermedia) display significant bactericidal effects against microorganisms such as Shigella flexneri, Staphylococcus aureus and E. coli even in the absence of active principles like linalool and linalyl acetate, considered responsible for the antibacterial and antifungal properties of essential oils obtained from different species of Lavandula. The results suggest once again that the antimicrobial activity of EOs is a resultant of the antibacterial properties of the major and minor components in their chemical composition. © 2013 Friends Science Publishers.

Harmanescu M.,Banats University of Agricultural science and Veterinary Medicine | Alda L.M.,Banats University of Agricultural science and Veterinary Medicine | Bordean D.M.,Banats University of Agricultural science and Veterinary Medicine | Gogoasa I.,Banats University of Agricultural science and Veterinary Medicine | Gergen I.,Banats University of Agricultural science and Veterinary Medicine
Chemistry Central Journal | Year: 2011

Background: The aim of this study is to measure the levels of heavy metals (Fe, Mn, Zn, Cu, Ni, Cd and Pb) found in common vegetables (parsley, carrot, onion, lettuce, cucumber and green beans) grown in contaminated mining areas compared with those grown in reference clear area and to determine their potential detrimental effects via calculation of the daily metal intake (DImetal) and Target Hazard Quotients (THQ) for normal daily consumption of these vegetables, for male and female gender.Results: Compared with the reference in contaminated areas, soil and plant contents of all analyzed metals are higher, usually over normally content for Mn, Zn, Cu, Cd and Pb. Particularly, in soil, higher values than intervention threshold values (ITV) were found for Cu and Pb and higher than maximum allowable limits (MAL) for Zn, Cu, Cd and Pb for parsley roots and leaves, carrot roots, cabbage, lettuce and cucumber. DImetal and THQ values for male and female were calculated for each vegetable and metal and for which oral reference doses exist. The combined THQ values calculated are concerning in that they are usually below the safe level of THQ<1 for all vegetables grown in reference area. In contaminated Moldova Noua (M) area the combined THQ exceeded the safe level only for parsley roots, while in more contaminated Ruschita (R) area combined THQ exceeded the safe level for parsley and carrot roots, lettuce and cabbage. Cd and Pb, most toxic metals to humans, have an increasing prevalence in the combined THQ for leafy (cabbage and lettuce) and fruit vegetables (cucumber). In the root vegetables only Pb has an increasing prevalence in combined THQ values. In all areas female THQ is higher than male THQ.Conclusion: The results of this study regarding metal contents in soils, vegetables, DImetal and THQ suggest that the consumption of some vegetables (especially parsley, carrot and cabbage and less for lettuce, cucumber and green beans) is not free of risks in these areas. The complex THQ parameter use in health risk assessment of heavy metals provides a better image than using only a simple parameter (contents of metals in soils and vegetables). © 2011 Harmanescu et al.

Mihoc M.,Banats University of Agricultural science and Veterinary Medicine | Pop G.,Banats University of Agricultural science and Veterinary Medicine | Alexa E.,Banats University of Agricultural science and Veterinary Medicine | Radulov I.,Banats University of Agricultural science and Veterinary Medicine
Chemistry Central Journal | Year: 2012

Background: The study aims to determine the nutritional value of hemp seed expressed by the oil content and by the concentration of metals (Ca, Mg, K, Fe, Mn, Zn and Cd), for five varieties of monoecious and dioecious hemp seeds approved in Romania, comparative with the concentration of these metals in the soil.Results: The content of oil in hempseed registers a slight decrease in the production records of 2011, losses due to drought and low levels of precipitation during the growth period. The greatest loss is found in Diana monoecious variety (26.54-20.82%) followed by Zenit varieties (27.37-22.97%), Armanca (29.27-25.32%), Silvana (28.89-25.04%) and Denise (26.96-25.30%). Siccative hemp oil has a yellowish green color and an iodine index of 140-156 g I2/100 g oil. Hemp seed are rich in mineral based Ca (144-955 mg/100 g seed), Mg (237-694 mg/100 g seed), K (463-2821 mg/100 g seed), Fe (1133-2400 mg.kg-1), Mn (63-110 mg.kg-1) and Zn (42-94 mg.kg-1). For the soil the following macroelements concentrations were determined: Ca (2100-2520 mg.kg-1), Mg (320-376 mg.kg-1) and K (232-257 mg.kg-1). Mn (156-197 mg.kg-1) and Zn (54-67 mg.kg-1) remain within normal limits for Romania. The soils in the experience area contain large amounts of Fe (19000-20430 mg.kg-1). The presence of K in large quantities determines the accumulation of large quantities of Fe in the soil.Conclusion: Hempseed belonging to the five Romanian varieties are rich source of nutrients (Ca, Mg, K) and unsaturated oil easily digestible by the body, but the presence of Cd concentrations above the upper limit puts a question mark over the use of seeds in various food products. Hemp extracts easily certain metals from the soil. Significant amounts of Fe (1133-2400 mg.kg-1), Mn (63-110 mg.kg-1), Zn (42-94 mg.kg-1) and Cd (1.3-4.0 mg.kg-1) are found in hemp seeds. Hemp (Cannabis sativa L.) is included among plants suitable for phytoremediation of soil contaminated with cadmium, zinc and iron. © 2012 Mihoc et al.; licensee Chemistry Central Ltd.

Hadaruga N.G.,Banats University of Agricultural science and Veterinary Medicine
Chemistry Central Journal | Year: 2012

Background: Obtaining new pharmaceutical materials with enhanced properties by using natural compounds and environment-friendly methods is a continuous goal for scientists. Ficaria verna Huds. is a widespread perennial plant with applications in the treat of haemorrhoids and to cure piles; it has also anti-inflammatory, astringent, and antibiotic properties. The goal of the present study is the obtaining and characterization of new F. verna extract/β-cyclodextrin complexes by using only natural compounds, solvents, and environment-friendly methods in order to increase the quality and acceptability versus toxicity indicator. Thus, the flavonoid content (as quercetin) of Ficaria verna Huds. flowers and leaves from the West side of Romania was determined and correlated with their antioxidant activity. Further, the possibility of obtaining β-cyclodextrin supramolecular systems was studied.Results: F. verna flowers and leaves extracts were obtained by semi-continuous solid-liquid extraction. The raw concentrated extract was spectrophotometrically analyzed in order to quantify the flavonoids from plant parts and to evaluate the antioxidant activity of these extracts. The F. verna extracts were used for obtaining β-cyclodextrin complexes; these were analyzed by scanning electron microscopy and Karl Fischer water titration; spectrophotometry was used in order to quantifying the flavonoids and evaluates the antioxidant activity. A higher concentration of flavonoids of 0.5% was determined in complexes obtained by crystallisation method, while only a half of this value was calculated for kneading method. The antioxidant activity of these complexes was correlated with the flavonoid content and this parameter reveals possible controlled release properties.Conclusions: The flavonoid content of F. verna Huds. from the West side of Romania (Banat county) is approximately the same in flowers and leaves, being situated at a medium value among other studies. β-Cyclodextrin complexes of F. verna extracts are obtained with lower yields by crystallisation than kneading methods, but the flavonoids (as quercetin) are better encapsulated in the first case most probably due to the possibility to attain the host-guest equilibrium in the slower crystallisation process. F. verna extracts and their β-cyclodextrin complexes have antioxidant activity even at very low concentrations and could be used in proper and valuable pharmaceutical formulations with enhanced bioactivity. © 2012 Hǎdǎrugǎ.

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