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Hadaruga N.G.,Banats University of Agricultural science and Veterinary Medicine | Hadaruga D.I.,Polytechnic University of Mozambique | Isengard H.-D.,University of Hohenheim
Food Chemistry | Year: 2012

The paper presents a comparative study regarding the water determination in natural cyclodextrins and in their essential oil complexes (Apiaceae, Liliaceae, and Cupressaceae families) by using Karl Fischer titration (KFT) and thermal methods. For the natural cyclodextrins, the influence of the solvent hydrophobicity and the preheating temperature on the water extraction process were evaluated. The water contents, estimated by KFT in both methanol and methanol-octanol solvent systems, were 10.6% and 14.4% for α- and β-cyclodextrin, respectively; the water content, estimated by KFT in a more hydrophilic solvent system, methanol-formamide, was 0.4-0.6% higher. Thermogravimetric evaluation of water conducts to lower values. For the essential oil/cyclodextrin complexes, the KFT water content were in the range of 6.4-8.1%, higher values being obtained in the case of Juniperus essential oil/β-cyclodextrin complexes (7.5-8.1%). With some exceptions, thermal analyses of complexes are in good agreement with the KFT results. © 2011 Elsevier Ltd. All rights reserved. Source


Iuliana C.,University of Veterinary and Animal Sciences | Cerasela P.,Banats University of Agricultural science and Veterinary Medicine
Romanian Biotechnological Letters | Year: 2014

Among the non-ionizing radiations, the ultraviolet radiations (UV) are the only ones which can change the genetic system. The investigations related to the soma clonal variability for potato (Solanum tuberosum) were focused on the response to the radiation exposure UV-C (λ = 254nm). The biological material used was represented by three types of potato: Cristian, Roclas and Rustic. For this purpose we used several RAPD markers (Random Amplified Polymorphic DNA). After the irradiation with UV-C there were some changes at molecular level by the occurrence in some cases of new DNA bands or the disappearance of DNA bands in other cases highlighted using three primers (OPF-12, OPW-11 and OPX-01). © 2014 University of Bucharest. Source


Sorina P.,Banats University of Agricultural science and Veterinary Medicine | Oana-Maria B.,Banats University of Agricultural science and Veterinary Medicine | Dorica B.,Banats University of Agricultural science and Veterinary Medicine
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM | Year: 2014

The deciphering of the metabolic pathway which induce the multileaflet trait in alfalfa it is of great importance due to the economic importance of the high biomass and the increased protein content. For this purpose the knox genes involved in meristem development and leaf morphogenesis were investigated. Thus, the knox genes expression were comparative analyzed for trileaflets plant and multileaflet ones in order to determine their specific involvement in leaf development using the semi quantitative one-step RT-PCR method. Four types of tissues were investigated: shoot tips, young leaves, old leaves and roots to determine the knox genes function in different stages of tissues development. It turned out that the average of the knox genes expression for all of the experimental variants was significantly higher for the mulileaflet plants, compared with the trileaflet ones. © SGEM2014. Source


Alexa E.,Sudan University of Science and Technology | Poiana M.-A.,Sudan University of Science and Technology | Sumalan R.-M.,Banats University of Agricultural science and Veterinary Medicine
International Journal of Molecular Sciences | Year: 2012

The aim of this study was to evaluate the effect of some natural extracts obtained from grape pomace (GPE) and grape seeds (GSE) as compared to a synthetic food, antioxidant-butylated hydroxytoluene (BHT), in order to control fungal population and ochratoxin A (OTA) production in naturally contaminated wheat. The results showed that the addition of these extracts was efficient with OTA content decreasing. On treatment with these extracts the loss of OTA content after 14 days was in the range 7.8-28.3% relative to the control sample, but increased up to 26.48-37% after 28 days. The highest loss in OTA content was recorded for treatment with GPE at the 500 ppm level. Regarding the fungal development, the obtained results show that the total fungal populations were significantly reduced by using natural extracts. The most efficient extract was GPE. Both BHT and GPE inhibit the growth of Penicillium verrrucosum, for all doses, but less with Aspergillus genera. GPE affects the growth of other types of moulds such as Rhizopus microsporus, Fusarium graminearum, Alternaria infectoria and Cladosporium herbarum. Our data suggested that GPE and GSE are able to provide fungicidal and fungistatic protection and to control the OTA accumulation in wheat, at least in a similar manner to BHT. © 2012 by the authors. Source


Dorica B.,Banats University of Agricultural science and Veterinary Medicine | Vanda B.,Banats University of Agricultural science and Veterinary Medicine | Sorina P.,Banats University of Agricultural science and Veterinary Medicine | Oana-Maria B.,Banats University of Agricultural science and Veterinary Medicine
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM | Year: 2014

In vitro cells culture is a good method to induce somatic variability in plants. The selection of plant tissues with a good capacity of cellular biosynthesis is necessary to obtain the important secondary metabolites by in vitro culture system. In our experiments we used biochemical methods to evaluate the variability induced by in vitro callus culture at three blueberry populations native in Romania. Callus obtained from Arieseni, Retezat and Valea Sebeşului populations, on WPM medium was sub-cultured in presence of ANA, BAP and AS. Antioxidant capacity using the FRAP method, glutathion (GSH), total anthocyanin, macroelements, microelements and heavy metals contents from callus lines selected were investigated. The AS concentration positively affects the activity of glutathione, with a significantly positive correlation coefficient (r = 0.54, p <0.05). The use of this hormone facilitates the obtaining of blueberry callus biomass with an increased antioxidant activity. There was also found a strong positive correlation between the total content of anthocyanin and FRAP values (r = 0.60, p <0.05) and a positive correlation with the level of GSH, respectively (r = 0.47, p <0.05). © SGEM2014. Source

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