Banats University Of Agricultural Science

Roman, Romania

Banats University Of Agricultural Science

Roman, Romania

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Bagiu R.V.,Victor Babes University of Medicine and Pharmacy Timisoara | Vlaicu B.,Victor Babes University of Medicine and Pharmacy Timisoara | Butnariu M.,Banats University of Agricultural science
International Journal of Molecular Sciences | Year: 2012

The objective of the study was to summarize the methods for isolating and identifying natural sulfur compounds from Allium ursinum (ramson) and to discuss the active constituents with regard to antifungal action. Using chromatographic techniques, the active constituents were isolated and subsequently identified. Analyses by high-performance liquid chromatography (HPLC) suggested that these compounds were sulfur constituents, with a characteristic absorbance at 250 nm. Gas chromatography-mass spectrometry (GC-MS) analyses allowed the chemical structures of the isolated constituents to be postulated. We adopted the same methods to identify the health-giving profiling of ramsons and the effects are thought to be primarily derived from the presence and breakdown of the alk(en)ylcysteine sulphoxide, alliin and its subsequent breakdown to allicin (sulfur-compounds of ramson) in connection with antifungal action. The aim of the study was the characterization of the chemical composition of ramsons and the testing of the action of the in vitro extracts, on different strains of Candida albicans. The main goal was to highlight the most efficient extracts of Allium ursinum that can provide long-term antifungal activity without remissions. The extracts from Allium ursinum plants, inhibited growth of Candida spp. cells at concentrations ranging from 0.5 to 4.0 mg/mL, while that of adherent cells at concentrations ranging from 1.0 to > 4.0 mg/mL, depending on the yeast and plant species. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Morar D.,Banats University of Agricultural science | Darabus G.,Banats University of Agricultural science | Imre M.,Banats University of Agricultural science | Ilie M.S.,Banats University of Agricultural science | Imre K.,University of Veterinary and Animal Sciences
Veterinary Clinical Pathology | Year: 2015

This case study describes the first genetically confirmed and clinically manifested autochthonous Ehrlichia canis infection in a 9-year-old female mixed-breed dog from Romania. Health screening of the dog included clinical examination, evaluation of stained peripheral blood smear and hematologic variables, as well as serologic testing and molecular analysis. Clinical signs included fever, apathy, dehydration, pale mucous membranes, and weakness. The microscopic examination of the blood smear and immunologic assays for Borrelia burgdorferi, Anaplasma phagocytophilum, and E canis antibodies, and for Dirofilaria immitis antigen yielded negative results. Hematologic abnormalities included moderate nonregenerative anemia, leucopenia with neutropenia, and moderate thrombocytopenia. The biochemical abnormalities identified were hypoalbuminemia, and mildly increased serum enzyme activities of AST and ALT. In addition, increased urea and creatinine levels associated with low urine specific gravity and proteinuria were also present. Nested PCR amplification of the partial E canis 16S rRNA gene demonstrated the presence of this rickettsial pathogen in the dog's blood, which subsequently was confirmed through sequencing based on the 100% homology with GenBank deposited E canis isolates. After specific treatment with doxycycline (10 mg/kg, orally, SID) for one month, the proteinuria, and hematologic and serum biochemical abnormalities with the exception of mild azotemia resolved. This report supports the geographical expansion of canine ehrlichiosis caused by E canis in nonendemic regions of Europe. © 2015 American Society for Veterinary Clinical Pathology.

Bandas C.,Romanian National Institute for Research and Development in Electrochemistry and Condensed Matter | Bandas C.,National Institute for Research and Development in Microtechnologies | Orha C.,Romanian National Institute for Research and Development in Electrochemistry and Condensed Matter | Misca C.,Banats University of Agricultural science | And 2 more authors.
Environmental Engineering and Management Journal | Year: 2013

A comparative study regarding the shape, dimension and bactericidal activity of the undoped and Ag-doped TiO2 nanocrystals was achieved. In this way, two hydrothermal methods, classical hydrothermal and microwave-assisted hydrothermal, was used for nanoparticles synthesis. The obtained materials were investigated by X-ray diffraction, DRUV-VIS spectroscopy, BET and SEM/EDX analysis. Antibacterial activity of undoped and Ag-doped TiO2 materials was tested on real water from Bega River. The best bactericidal activity was proved by using Ag-TiO2 catalyst synthesized by microwave-assisted hydrothermal method, through damaging of E. coli colonies under visible irradiation.

Posta D.S.,Banats University of Agricultural Science | Camen D.,Banats University of Agricultural Science | Radulov I.,Banats University of Agricultural Science | Berbecea A.,Banats University of Agricultural Science
Revista de Chimie | Year: 2015

Leaf and soil were sampled from three parks where there are lots of visitors and lots of pollution because of the heavy traffic and large numbers of cars and because of atmospheric pollution caused by the large companies. In this study on the heavy metals accumulated in the leaves and soil we chose the species Betula pendula Roth. because it has been planted intensely both in groups and isolated in the three locations and because they fix heavy metals in areas polluted by both soil and air.

Butnariu M.,Banats University of Agricultural science | Coradini C.Z.,Banats University of Agricultural science
Chemistry Central Journal | Year: 2012

Background: This study aimed to quantify the active biological compounds in C. officinalis flowers. Based on the active principles and biological properties of marigolds flowers reported in the literature, we sought to obtain and characterize the molecular composition of extracts prepared using different solvents. The antioxidant capacities of extracts were assessed by using spectrophotometry to measure both absorbance of the colorimetric free radical scavenger 2,2-diphenyl-1-picrylhydrazyl (DPPH) as well as the total antioxidant potential, using the ferric reducing power (FRAP) assay. Results: Spectrophotometric assays in the ultraviolet-visible (UV-VIS) region enabled identification and characterization of the full range of phenolic and flavonoids acids, and high-performance liquid chromatography (HPLC) was used to identify and quantify phenolic compounds (depending on the method of extraction). Methanol ensured more efficient extraction of flavonoids than the other solvents tested. Antioxidant activity in methanolic extracts was correlated with the polyphenol content. Conclusions: The UV-VIS spectra of assimilator pigments (e.g. chlorophylls), polyphenols and flavonoids extracted from the C. officinalis flowers consisted in quantitative evaluation of compounds which absorb to wavelengths broader than 360 nm. © 2010 Butnariu et al.

Butnariu M.,Banats University of Agricultural science
Chemistry Central Journal | Year: 2012

Background: This study aimed to quantify the allelopathic potential of Datura stramonium (Jimson weed). Sorghum halepense (Johnsongrass) tolerance was assessed by germinating, seed and growing seedlings, dosing of photo-synthesis pigments, followed by treatment with D. stramonium extract tropane alkaloids.Results: Preliminary chemical analysis of the extracts showed the presence of alkaloids.The presence of alkaloids was confirmed through HPLC-UV system analysis. Various concentrations of analytic purity alkaloids had similar effects on germination and development of S. halepense's root systems with those of extracts from of D. stramonium. Germination was not affected by any of the tested extracts, but growth was inhibited by the presence of tropane alkaloids. Extracts had effects at higher alkaloid concentrations. Seedlings of S. halepense developed toxicity symptoms in the presence of alkaloid extracts, but the occurrence of several chlorotic and necrotic areas was noticed in the flower extract biotest.Conclusions: Results show that the tested species is sensitive to alkaloids in their growth environment. This research justifies the fact that aqueous extracts from D. stramonium are adequate to the situations in which S. halepense becomes damaging. © 2012 Butnariu; licensee Chemistry Central Ltd.

Nica D.V.,University of Veterinary and Animal Sciences | Bura M.,University of Veterinary and Animal Sciences | Gergen I.,Sudan University of Science and Technology | Harmanescu M.,Banats University of Agricultural science | Bordean D.-M.,Sudan University of Science and Technology
Chemistry Central Journal | Year: 2012

Background: Copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) can pose serious threats to environmental health because they tend to bioaccumulate in terrestrial ecosystems. We investigated under field conditions the transfer of these heavy metals in a soil-plant-snail food chain in Banat area, Romania. The main goal of this paper was to assess the Roman snail (Helix pomatia) usefulness in environmental monitoring as bioindicator of heavy metal accumulation. Eight sampling sites, selected by different history of heavy metal (HM) exposure, were chosen to be sampled for soil, nettle leaves, and newly matured snails. This study also aimed to identify the putative effects of HM accumulation in the environment on phenotypic variability in selected shell features, which included shell height (SH), relative shell height (RSH), and whorl number (WN).Results: Significantly higher amounts of HMs were accumulated in snail hepatopancreas and not in foot. Cu, Zn, and Cd have biomagnified in the snail body, particularly in the hepatopancreas. In contrast, Pb decreased when going up into the food chain. Zn, Cd, and Pb correlated highly with each other at all levels of the investigated food chain. Zn and Pb exhibited an effective soil-plant transfer, whereas in the snail body only foot Cu concentration was correlated with that in soil. There were significant differences among sampling sites for WN, SH, and RSH when compared with reference snails. WN was strongly correlated with Cd and Pb concentrations in nettle leaves but not with Cu and Zn. SH was independent of HM concentrations in soil, snail hepatopancreas, and foot. However, SH correlated negatively with nettle leaves concentrations for each HM except Cu. In contrast, RSH correlated significantly only with Pb concentration in hepatopancreas.Conclusions: The snail hepatopancreas accumulates high amounts of HMs, and therefore, this organ can function as a reliable biomarker for tracking HM bioavailability in soil. Long-term exposure to HMs via contaminated food might influence the variability of shell traits in snail populations. Therefore, our results highlight the Roman snail (Helix pomatia) potential to be used in environmental monitoring studies as bioindicator of HM pollution. © 2012 Nica et al.; licensee Chemistry Central Ltd.

Background: The aim of our work was to compare two methods, both based on direct transmethylation with different reagents, BF 3/MeOH (boron trifluoride in methanol) or HCl/MeOH (hydrochloride acid in methanol), in acid catalysis, without prior extraction, to find the fast, non-expensive but enough precise method for 9 principal fatty acids (lauric, myristic, palmitic, stearic, oleic, linoleic, linolenic, arahidic and behenic acids) analysis in vegetal matrix with low fat content (forage from grassland), for nutrition and agrochemical studies.Results: Comparatively, between the average values obtained for all analysed fatty acids by the two methods based on direct transmethylation without prior extraction no significantly difference was identified (p > 0.05). The results of fatty acids for the same forage sample were more closely to their average value, being more homogenous for BF 3/MeOH than HCl/MeOH, because of the better accuracy and repeatability of this method. Method that uses BF 3/MeOH reagent produces small amounts of interfering compounds than the method using HCl/MeOH reagent, results reflected by the better statistical parameters.Conclusion: The fast and non-expensive BF 3/methanol method was applied with good accuracy and sensitivity for the determination of free or combined fatty acids (saturated and unsaturated) in forage matrix with low fat content from grassland. Also, the final extract obtained by this method, poorer in interfering compounds, is safer to protect the injector and column from contamination with heavy or non-volatile compounds formed by transmethylation reactions. © 2011 Harmanescu.

Alina G.,Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara | Grozea I.,Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara | Virteiu A.M.,Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara | Baghina N.,Banats University of Agricultural science
Journal of Food, Agriculture and Environment | Year: 2013

Metcalfa pruinosa Say (1830) (Insecta:Homoptera:Flatidae), most commonly known as flatid plant hopper, is a relatively new insect pest for Eastern Europe. In Romania, the first recorded of species was in 2009 in Constanta, in an isolated eastern area; the second recorded was in 2010 in Timisoara area at a long distance and opposite from the first point. The main purpose of this paper is to detect new areas infested and determine the range of the host plants. Researches made in the west part of Romania for over 2 years (2011 and 2012), and covered green spaces between buildings, public green parks, orchards and vineyards. Observation points have been established as the western border counties. Their choice has been taking into account of ecological preferences of pest and geographical coordinates of studied zone. Host plants of flatid vary from area to area including herbaceous or woody, ornamental or agricultural plants. The most visible damage were observed in follows plant species: Acer sp., Tilia cordata, Rhus typhina, Carpinus betulus, Catalpa bignonioides, Ficus carica, Hibiscus syriacus, Juglans nigra, Ulmus minor, Rosa sp, Philadelphus sp., Cornus sp., Vitis vinifera, Prunus armeniaca, Prunus persica, Malus domestica. Most plants affected are ornamentals species but agricultural plants from vineyards and orchards highlights a significant increase in both the level of damage and species. The colonies of adults and larvae are located usually on leaves and shoots, fruits and grapes clusters. The damage caused may directly and indirectly affect the growth and development of plants. Rapid expansion in the last two years and the poliphagous nature require attention from specialists for limiting their spread.

Hadaruga D.I.,Polytechnic University of Timişoara | Unlusayin M.,Akdeniz University | Gruia A.T.,County Clinical Emergency Hospital Timisoara | Birau C.,Banats University of Agricultural science | And 2 more authors.
Beilstein Journal of Organic Chemistry | Year: 2016

The thermal and oxidative stability of Atlantic salmon oil (Salmo salar L.) as well as its β-cyclodextrin (β-CD) complexation ability has been verified for the first time. The main omega-3 fatty acids, EPA and DHA, were significantly degraded, even at 50 °C. Their relative concentrations decrease from 6.1% for EPA and 4.1% for DHA to 1.7% and 1.5% after degradation at 150 °C, respectively. On the other hand, the relative concentrations of monounsaturated and saturated fatty acids remained constant or slightly increased by a few percent after degradation (e.g., from 10.7% to 12.9% for palmitic acid). Co-crystallization of ASO with β-CD at a host-guest ratio of 1:1 and 3:1 from an ethanol-water mixture and kneading methods has been used for the preparation of β-CD/ASO complexes. The analysis of the complexes by thermogravimetry, differential scanning calorimetry (DSC), and Karl Fischer titration (KFT) as well as the decrease of the "strongly-retained" water content confirm the formation of the inclusion compound. Furthermore, the DSC parameters correlate well with the KFT kinetic data for β-CD/ASO complexes. © 2016 Hǎdǎrugǎ et al; licensee Beilstein-Institut. License and terms: see end of document.

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