Banats University Of Agricultural Science

Roman, Romania

Banats University Of Agricultural Science

Roman, Romania
Time filter
Source Type

Rashed K.N.,National Research Center of Egypt | Butnariu M.,Banats University of Agricultural science
Advanced Pharmaceutical Bulletin | Year: 2014

Purpose: The present study was carried out to evaluate antimicrobial and antioxidant activities from Eriobotrya japonica stems as well investigation of its chemical composition. Methods: Methanol 80% extract of Eriobotrya japonica stems was tested for antimicrobial activity against bacterial and fungal strains and for antioxidant activity using oxygen radical absorbance capacity (ORAC) and the trolox equivalent antioxidant capacity (TEAC) assays and also total content of polyphenols with phytochemical analysis of the extract were determined. Results: The results showed that the extract has a significant antimicrobial activity, it inhibited significantly the growth of Candida albicans suggesting that it can be used in the treatment of fungal infections, and it showed no effect on the other bacterial and fungal strains, the extract has a good antioxidant activity, it has shown high values of oxygen radical absorbance capacity and trolox equivalent antioxidant capacity, while it showed a low value of polyphenol content. Phytochemical analysis of the extract showed the presence of carbohydrates, terpenes, tannins and flavonoids, further phytochemical analysis resulted in the isolation and identification of three triterpenic acids, oleanolic, ursolic and corosolic acids and four flavonoids, naringenin, quercetin, kaempferol 3-O-β-glucoside and quercetin 3-O-α-rhamnoside. Conclusion: These results may help to discover new chemical classes of natural antimicrobial antioxidant substances. © 2014 by Tabriz University of Medical Sciences.

Silviu-Adrian B.,Banats University of Agricultural science | Monica N.,Banats University of Agricultural science | Teodor-Ioan T.,Banats University of Agricultural science
Carpathian Journal of Food Science and Technology | Year: 2017

The purpose of the paper is to present the study regarding the influence of natural extracts (Kombucha extracts) on the nutritional quality of polish sausages comparative with polish sausages with chemical additives. The products (classic polish sausages with chicken meat and polish sausages with chicken meat and kombucha extracts) were obtained at a private company from Timisoara and the quality control of the studied products were performed at the laboratory for physic-chemical and bacteriological analyzes at the same company. The physic chemical analyzes were done according to the following standars: ISO 1442/1997 (water content), SR ISO 1444/2008 (fat content), STAS 9065/5-1973 (sodium chloride content), SR ISO 937:2007 (nitrogen content), SR ISO 17025 (Kreiss reaction), STAS 9065/9-74 (nitrites content). The obtained results show that the natural extracts (Kombucha extract) used in polish sausages with chicken meat have a high influence on the physic-chemical parameters, the obtained product has high nutritional value, without chemical additives.

Morariu S.,Banats University of Agricultural science | Mederle N.,Banats University of Agricultural science | Badea C.,Banats University of Agricultural science | Darabus G.,Banats University of Agricultural science | And 2 more authors.
Veterinary Parasitology | Year: 2016

Forty seven working horses from Romania were post-mortem examined for small strongyles (Cyathostominae) infections. All horses were found infected. The overall cyathostomins intensity ranged from 390 to 13,010 and horses were infected by 8-24 species. The intensity was higher in ventral colon (1531) and dorsal colon (824), the lowest in the caecum (524). Twenty four species were identified. Cyathostomum catinatum, Cylicocyclus insigne, and C. Nassatus had 100% of prevalence. Over 50% of horses were infected by Coronocyclus coronatus, Cylicostephanus calicatus, C. goldi, and C. longibursatus. Other prevalent species (34%-45%) were Cyathostomum tetracanthum, Cylicostephanus minutus and Gyalocephalus capitatus. Coronocyclus labiatus, Parapoteriostomum mettami, Poteriostomum imparidentatum and P. ratzii had the lowest prevalence. Most species showed high organ preference with a niche breadth value between 1 and 1.96 while only 7 species (Coronocyclus labiatus, Cyathostomum tetracanthum, C. brevicapsulatus, Cylicocyclus elongatus, C. insigne, C. leptostomum and C. radiatus) showed a more generalist selection. The niche breadth of 10 species was significantly (p < 0.05) influenced by itself intensity (Coronocyclus labratus, Cyathostomum pateratum, C. tetracanthum, Cylicocyclus elongatus, C. radiatus, C. ultrajectinus, C. leptostomum, Cylicodontophorus euproctus, Poteriostomum imparidentatum, P. ratzii). The niche breadth of Cylicocyclus nassatus was positively (p < 0.05) influenced by the summed intensity of the other species while that of Cylicocyclus elongatus was negatively (p < 0.05) influenced by the intensity of the other species. The cluster analysis of the Cyathostominae community composition showed a major cluster composed by the three dominant species, followed by a cluster composed by Coronocyclus coronatus, while all the other species presented a tree like structure. © 2016 Elsevier B.V.

Bagiu R.V.,Victor Babes University of Medicine and Pharmacy Timisoara | Vlaicu B.,Victor Babes University of Medicine and Pharmacy Timisoara | Butnariu M.,Banats University of Agricultural science
International Journal of Molecular Sciences | Year: 2012

The objective of the study was to summarize the methods for isolating and identifying natural sulfur compounds from Allium ursinum (ramson) and to discuss the active constituents with regard to antifungal action. Using chromatographic techniques, the active constituents were isolated and subsequently identified. Analyses by high-performance liquid chromatography (HPLC) suggested that these compounds were sulfur constituents, with a characteristic absorbance at 250 nm. Gas chromatography-mass spectrometry (GC-MS) analyses allowed the chemical structures of the isolated constituents to be postulated. We adopted the same methods to identify the health-giving profiling of ramsons and the effects are thought to be primarily derived from the presence and breakdown of the alk(en)ylcysteine sulphoxide, alliin and its subsequent breakdown to allicin (sulfur-compounds of ramson) in connection with antifungal action. The aim of the study was the characterization of the chemical composition of ramsons and the testing of the action of the in vitro extracts, on different strains of Candida albicans. The main goal was to highlight the most efficient extracts of Allium ursinum that can provide long-term antifungal activity without remissions. The extracts from Allium ursinum plants, inhibited growth of Candida spp. cells at concentrations ranging from 0.5 to 4.0 mg/mL, while that of adherent cells at concentrations ranging from 1.0 to > 4.0 mg/mL, depending on the yeast and plant species. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Morar D.,Banats University of Agricultural science | Darabus G.,Banats University of Agricultural science | Imre M.,Banats University of Agricultural science | Ilie M.S.,Banats University of Agricultural science | Imre K.,University of Veterinary and Animal Sciences
Veterinary Clinical Pathology | Year: 2015

This case study describes the first genetically confirmed and clinically manifested autochthonous Ehrlichia canis infection in a 9-year-old female mixed-breed dog from Romania. Health screening of the dog included clinical examination, evaluation of stained peripheral blood smear and hematologic variables, as well as serologic testing and molecular analysis. Clinical signs included fever, apathy, dehydration, pale mucous membranes, and weakness. The microscopic examination of the blood smear and immunologic assays for Borrelia burgdorferi, Anaplasma phagocytophilum, and E canis antibodies, and for Dirofilaria immitis antigen yielded negative results. Hematologic abnormalities included moderate nonregenerative anemia, leucopenia with neutropenia, and moderate thrombocytopenia. The biochemical abnormalities identified were hypoalbuminemia, and mildly increased serum enzyme activities of AST and ALT. In addition, increased urea and creatinine levels associated with low urine specific gravity and proteinuria were also present. Nested PCR amplification of the partial E canis 16S rRNA gene demonstrated the presence of this rickettsial pathogen in the dog's blood, which subsequently was confirmed through sequencing based on the 100% homology with GenBank deposited E canis isolates. After specific treatment with doxycycline (10 mg/kg, orally, SID) for one month, the proteinuria, and hematologic and serum biochemical abnormalities with the exception of mild azotemia resolved. This report supports the geographical expansion of canine ehrlichiosis caused by E canis in nonendemic regions of Europe. © 2015 American Society for Veterinary Clinical Pathology.

Butnariu M.,Banats University of Agricultural science | Coradini C.Z.,Banats University of Agricultural science
Chemistry Central Journal | Year: 2012

Background: This study aimed to quantify the active biological compounds in C. officinalis flowers. Based on the active principles and biological properties of marigolds flowers reported in the literature, we sought to obtain and characterize the molecular composition of extracts prepared using different solvents. The antioxidant capacities of extracts were assessed by using spectrophotometry to measure both absorbance of the colorimetric free radical scavenger 2,2-diphenyl-1-picrylhydrazyl (DPPH) as well as the total antioxidant potential, using the ferric reducing power (FRAP) assay. Results: Spectrophotometric assays in the ultraviolet-visible (UV-VIS) region enabled identification and characterization of the full range of phenolic and flavonoids acids, and high-performance liquid chromatography (HPLC) was used to identify and quantify phenolic compounds (depending on the method of extraction). Methanol ensured more efficient extraction of flavonoids than the other solvents tested. Antioxidant activity in methanolic extracts was correlated with the polyphenol content. Conclusions: The UV-VIS spectra of assimilator pigments (e.g. chlorophylls), polyphenols and flavonoids extracted from the C. officinalis flowers consisted in quantitative evaluation of compounds which absorb to wavelengths broader than 360 nm. © 2010 Butnariu et al.

Butnariu M.,Banats University of Agricultural science
Chemistry Central Journal | Year: 2012

Background: This study aimed to quantify the allelopathic potential of Datura stramonium (Jimson weed). Sorghum halepense (Johnsongrass) tolerance was assessed by germinating, seed and growing seedlings, dosing of photo-synthesis pigments, followed by treatment with D. stramonium extract tropane alkaloids.Results: Preliminary chemical analysis of the extracts showed the presence of alkaloids.The presence of alkaloids was confirmed through HPLC-UV system analysis. Various concentrations of analytic purity alkaloids had similar effects on germination and development of S. halepense's root systems with those of extracts from of D. stramonium. Germination was not affected by any of the tested extracts, but growth was inhibited by the presence of tropane alkaloids. Extracts had effects at higher alkaloid concentrations. Seedlings of S. halepense developed toxicity symptoms in the presence of alkaloid extracts, but the occurrence of several chlorotic and necrotic areas was noticed in the flower extract biotest.Conclusions: Results show that the tested species is sensitive to alkaloids in their growth environment. This research justifies the fact that aqueous extracts from D. stramonium are adequate to the situations in which S. halepense becomes damaging. © 2012 Butnariu; licensee Chemistry Central Ltd.

Nica D.V.,University of Veterinary and Animal Sciences | Bura M.,University of Veterinary and Animal Sciences | Gergen I.,Sudan University of Science and Technology | Harmanescu M.,Banats University of Agricultural science | Bordean D.-M.,Sudan University of Science and Technology
Chemistry Central Journal | Year: 2012

Background: Copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) can pose serious threats to environmental health because they tend to bioaccumulate in terrestrial ecosystems. We investigated under field conditions the transfer of these heavy metals in a soil-plant-snail food chain in Banat area, Romania. The main goal of this paper was to assess the Roman snail (Helix pomatia) usefulness in environmental monitoring as bioindicator of heavy metal accumulation. Eight sampling sites, selected by different history of heavy metal (HM) exposure, were chosen to be sampled for soil, nettle leaves, and newly matured snails. This study also aimed to identify the putative effects of HM accumulation in the environment on phenotypic variability in selected shell features, which included shell height (SH), relative shell height (RSH), and whorl number (WN).Results: Significantly higher amounts of HMs were accumulated in snail hepatopancreas and not in foot. Cu, Zn, and Cd have biomagnified in the snail body, particularly in the hepatopancreas. In contrast, Pb decreased when going up into the food chain. Zn, Cd, and Pb correlated highly with each other at all levels of the investigated food chain. Zn and Pb exhibited an effective soil-plant transfer, whereas in the snail body only foot Cu concentration was correlated with that in soil. There were significant differences among sampling sites for WN, SH, and RSH when compared with reference snails. WN was strongly correlated with Cd and Pb concentrations in nettle leaves but not with Cu and Zn. SH was independent of HM concentrations in soil, snail hepatopancreas, and foot. However, SH correlated negatively with nettle leaves concentrations for each HM except Cu. In contrast, RSH correlated significantly only with Pb concentration in hepatopancreas.Conclusions: The snail hepatopancreas accumulates high amounts of HMs, and therefore, this organ can function as a reliable biomarker for tracking HM bioavailability in soil. Long-term exposure to HMs via contaminated food might influence the variability of shell traits in snail populations. Therefore, our results highlight the Roman snail (Helix pomatia) potential to be used in environmental monitoring studies as bioindicator of HM pollution. © 2012 Nica et al.; licensee Chemistry Central Ltd.

Background: The aim of our work was to compare two methods, both based on direct transmethylation with different reagents, BF 3/MeOH (boron trifluoride in methanol) or HCl/MeOH (hydrochloride acid in methanol), in acid catalysis, without prior extraction, to find the fast, non-expensive but enough precise method for 9 principal fatty acids (lauric, myristic, palmitic, stearic, oleic, linoleic, linolenic, arahidic and behenic acids) analysis in vegetal matrix with low fat content (forage from grassland), for nutrition and agrochemical studies.Results: Comparatively, between the average values obtained for all analysed fatty acids by the two methods based on direct transmethylation without prior extraction no significantly difference was identified (p > 0.05). The results of fatty acids for the same forage sample were more closely to their average value, being more homogenous for BF 3/MeOH than HCl/MeOH, because of the better accuracy and repeatability of this method. Method that uses BF 3/MeOH reagent produces small amounts of interfering compounds than the method using HCl/MeOH reagent, results reflected by the better statistical parameters.Conclusion: The fast and non-expensive BF 3/methanol method was applied with good accuracy and sensitivity for the determination of free or combined fatty acids (saturated and unsaturated) in forage matrix with low fat content from grassland. Also, the final extract obtained by this method, poorer in interfering compounds, is safer to protect the injector and column from contamination with heavy or non-volatile compounds formed by transmethylation reactions. © 2011 Harmanescu.

Hadaruga D.I.,Polytechnic University of Timişoara | Unlusayin M.,Akdeniz University | Gruia A.T.,County Clinical Emergency Hospital Timisoara | Birau C.,Banats University of Agricultural science | And 2 more authors.
Beilstein Journal of Organic Chemistry | Year: 2016

The thermal and oxidative stability of Atlantic salmon oil (Salmo salar L.) as well as its β-cyclodextrin (β-CD) complexation ability has been verified for the first time. The main omega-3 fatty acids, EPA and DHA, were significantly degraded, even at 50 °C. Their relative concentrations decrease from 6.1% for EPA and 4.1% for DHA to 1.7% and 1.5% after degradation at 150 °C, respectively. On the other hand, the relative concentrations of monounsaturated and saturated fatty acids remained constant or slightly increased by a few percent after degradation (e.g., from 10.7% to 12.9% for palmitic acid). Co-crystallization of ASO with β-CD at a host-guest ratio of 1:1 and 3:1 from an ethanol-water mixture and kneading methods has been used for the preparation of β-CD/ASO complexes. The analysis of the complexes by thermogravimetry, differential scanning calorimetry (DSC), and Karl Fischer titration (KFT) as well as the decrease of the "strongly-retained" water content confirm the formation of the inclusion compound. Furthermore, the DSC parameters correlate well with the KFT kinetic data for β-CD/ASO complexes. © 2016 Hǎdǎrugǎ et al; licensee Beilstein-Institut. License and terms: see end of document.

Loading Banats University Of Agricultural Science collaborators
Loading Banats University Of Agricultural Science collaborators