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Rashed K.,National Research Center of Egypt | Butnariu M.,Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara
Iranian Journal of Pharmaceutical Research

Methanol 70% extract of Bauhinia racemosa aerial parts was tested for antimicrobial activity against different bacterial and fungal strains and for antioxidant activity and also total content of polyphenols with phytochemical analysis of the extract was determined. The results have showed that the extract has a significant antimicrobial activity, it inhibited the growth of Bacillus subtilis and also it was highly active against Candida albicans suggesting that it can be used in the treatment of fungal infections. The extract has a good antioxidant activity, it has shown high values of the trolox equivalent antioxidant capacity and polyphenol content while it has shown a lower value of oxygen radical absorbance capacity. Phytochemical analysis has shown that it has interesting phytochemical bioconstituents, include flavonoids, tannins and others, and the deep phytochemical study results in the isolation of methyl gallate, gallic, kaempferol, quercetin, querection 3-O-α-rhamnoside, kaempferol 3-O-β-glucoside, myricetin-3-O-β- glucoside, querection-3-O-rutinoside (Rutin). All compounds were identified by different spectroscopic analyses (UV, 1H-NMR, 13C-NMR) and Mass Spectrometry (MS). © 2014 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services. Source

Butnariu M.V.,Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara | Giuchici C.V.,Inspectorate for quality of seed and planting materials
Journal of Nanobiotechnology

Background: The use of natural products based on aqueous extract of propolis and lycopene in the skin's protective mechanisms against UVA radiation was evaluated by means of experimental acute inflammation on rat paw edema. The aim of the present study was to evaluate the harmlessness of propolis - lycopene system through evaluation of skin level changes and anti-inflammatory action. The regenerative and protective effect of the aqueous propolis and lycopene extract is based on its richness in biologically active substances such as: tocopherols, flavonoids, amino acids, polyunsaturated fatty acids, the chlorophyll pigment, all substances with strong antioxidant activity, that modify the oxidative stress, mainly by reducing the prooxidant processes and enhancing the antioxidant ones. These substances participate in the synthesis of prostaglandins and phospholipids components of cell membrane thus enhancing skin protection mechanisms.Results: The experimental systems offered a sustained release of the drug, in vitro, for aim eight hours. The prepared formulations aim did not reveal a deteriorating effect on tissues. They proved a better therapeutic efficiency Compared to standard suspension, they provided a better therapeutic efficiency coupled with extended time interval of tested parameters (24 hours). Preliminary examination of tissues showed that the experimental formulations did not irritate. Local application of propolis and lycopene aqueous extract nanoemulsion has a high potential both regarding its efficiency (the analgesic effect) and therapeutic safety.Conclusions: This study demonstrates that propolis and lycopene extract nanoemulsions, preparations contains active substances, can confer better therapeutic effects than those of the conventional formulations, based on local control-release of dozed form, for a longer period of time, which probably improve its efficiency and skin acceptance, meaning a better compliance. The information obtained in the present study suggests that administration of propolis and lycopene aqueous extract nanoemulsion is safe. The preparation can be useful for further preclinical studies lycopene embedded in aqueous propolis extract to be used in pharmaceuticals (targeted medical therapy). © 2011 Butnariu and Giuchici; licensee BioMed Central Ltd. Source

Gergen I.,Sudan University of Science and Technology | Harmanescu M.,Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara
Chemistry Central Journal

Background: The aim of the paper is to assess by the principal components analysis (PCA) the heavy metal contamination of soil and vegetables widely used as food for people who live in areas contaminated by heavy metals (HMs) due to long-lasting mining activities. This chemometric technique allowed us to select the best model for determining the risk of HMs on the food chain as well as on people's health.Results: Many PCA models were computed with different variables: heavy metals contents and some agro-chemical parameters which characterize the soil samples from contaminated and uncontaminated areas, HMs contents of different types of vegetables grown and consumed in these areas, and the complex parameter target hazard quotients (THQ). Results were discussed in terms of principal component analysis.Conclusion: There were two major benefits in processing the data PCA: firstly, it helped in optimizing the number and type of data that are best in rendering the HMs contamination of the soil and vegetables. Secondly, it was valuable for selecting the vegetable species which present the highest/minimum risk of a negative impact on the food chain and human health. © 2012 Gergen and Harmanescu; licensee Chemistry Central Ltd. Source

Hadaruga N.G.,Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara
Chemistry Central Journal

Background: Obtaining new pharmaceutical materials with enhanced properties by using natural compounds and environment-friendly methods is a continuous goal for scientists. Ficaria verna Huds. is a widespread perennial plant with applications in the treat of haemorrhoids and to cure piles; it has also anti-inflammatory, astringent, and antibiotic properties. The goal of the present study is the obtaining and characterization of new F. verna extract/β-cyclodextrin complexes by using only natural compounds, solvents, and environment-friendly methods in order to increase the quality and acceptability versus toxicity indicator. Thus, the flavonoid content (as quercetin) of Ficaria verna Huds. flowers and leaves from the West side of Romania was determined and correlated with their antioxidant activity. Further, the possibility of obtaining β-cyclodextrin supramolecular systems was studied.Results: F. verna flowers and leaves extracts were obtained by semi-continuous solid-liquid extraction. The raw concentrated extract was spectrophotometrically analyzed in order to quantify the flavonoids from plant parts and to evaluate the antioxidant activity of these extracts. The F. verna extracts were used for obtaining β-cyclodextrin complexes; these were analyzed by scanning electron microscopy and Karl Fischer water titration; spectrophotometry was used in order to quantifying the flavonoids and evaluates the antioxidant activity. A higher concentration of flavonoids of 0.5% was determined in complexes obtained by crystallisation method, while only a half of this value was calculated for kneading method. The antioxidant activity of these complexes was correlated with the flavonoid content and this parameter reveals possible controlled release properties.Conclusions: The flavonoid content of F. verna Huds. from the West side of Romania (Banat county) is approximately the same in flowers and leaves, being situated at a medium value among other studies. β-Cyclodextrin complexes of F. verna extracts are obtained with lower yields by crystallisation than kneading methods, but the flavonoids (as quercetin) are better encapsulated in the first case most probably due to the possibility to attain the host-guest equilibrium in the slower crystallisation process. F. verna extracts and their β-cyclodextrin complexes have antioxidant activity even at very low concentrations and could be used in proper and valuable pharmaceutical formulations with enhanced bioactivity. © 2012 Hǎdǎrugǎ. Source

Jianu C.,Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara
Journal of Food, Agriculture and Environment

There is an increasing interest for the wide array of hygiene products within the food product's safety policies. Alkaline and ammonium salts of β- alkyl (C 12H 25/C 18H 37) (7/3) polyethyleneoxy (n = 3-20) propionic acids (nonionic soaps), based on their fundamental colloidal properties of the adjustable hydrophilic/hydrophobic balance, ensure future favorable synergies (foaming/antifoaming/dispersion/micelle solubility, wetting/display, washing, emulsion, sequestering/coordination capacities), diminishing the consequences of water hardness for processing, emollient, antifungal competences etc. The main colloidal competences (surface tension, critical micellar concentration) within the homologue series of hydrocarbon and heterogeneous polyoxyethylene chains (n) were analyzed in this study. Source

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