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Gautam R.,Indian Central Arid Zone Research Institute | Singh S.K.,Indian Central Arid Zone Research Institute | Sharma V.,Banasthali Vidhyapeeth.
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2014

Concurrent heat and moisture stress often favours root diseases caused by Macrophomina phaseolina in arid legumes. Molecular analysis revealed 92 % variation within M. phaseolina populations as compared to 8 % among populations. The first three principle coordinates of PCA analysis accounted for a 69.61 % of total variance and Eigen vectors revealed 22.89 % of total variability. The mean values of all the four populations together for Nei's gene diversity (h) was 0.1990 and Shannon's information index (i) was 0.3113. The result showed that the genetic diversity of M. phaseolina isolates of population 2 (cowpea) was richest among all the four populations. Analysis of molecular variance indicated that main proportion of genetic variation was within the host than among different hosts. Out of 13 representative isolates seven were molecularly identified as Rhizoctonia bataticola and six as M. phaseolina upon sequencing of 5.8S RNA region. Besides length variation in ITS-1, 5.8S rRNA gene, ITS-2 and total length, the authors report insertion/deletions at a number of places in 13 isolates sequenced. This study underlines that M. phaseolina distribution is independent of host and/or geography and validates the utility of ITS rDNA region as a reliable indicator of phylogenetic interrelationships as an additional approach for identification of the genus Macrophomina and assessing its genetic diversity. © 2013 The National Academy of Sciences, India.

Yadav P.,Jiwaji University | Koul K.K.,Jiwaji University | Srivastava N.,Banasthali Vidhyapeeth. | Mendki M.J.,Defense Research & Development Establishment | Bhagyawant S.S.,Jiwaji University
Plant Biosystems | Year: 2016

Twenty chickpea accessions belonging to ten different countries of the world have been subjected to phylogeny and length variations from nuclear ribosomal DNA (nr DNA). ITS1–5.8S–ITS2 regions of Cicer accessions were used for amplification in two sets, each comprising a reverse and forward ITS primers. Lengths of ITS-1 of C. arietinum and C. reticulatum ranged from 340 to 350 bp whereas that of ITS-2 from 400 to 410 bp. In all the 20 accessions investigated, GC content in ITS-1 ranged from 40 to 55% and in ITS-2 from 42 to 55%. Sequencing of polymerase chain reaction product from ITS-1 showed variability in 278 and 290 bp due to adenine and guanine nucleotide base pairs. BLAST search for ITS-1 region revealed highest homology (99%) with four strains of C. arietinum accessions. Whereas, ITS2 showed 100% homology with C. arietinum and 99% homology with that of C. echinospermum. © 2016 Società Botanica Italiana

Dhyani P.,Banasthali Vidhyapeeth. | Rishi O.P.,University of Kota
Proceedings - 2015 5th International Conference on Communication Systems and Network Technologies, CSNT 2015 | Year: 2015

Popularity and usability of Social Media in today's scenario is universal. None of us can deny to the fact it is not only the source of communication or connection between friends but also has broaden its horizons to politics, television industry, source to advertisement for companies, education and many more[11][15]. Most of the companies or personalities are maintaining pages on these social media sites to interact with the users of these sites they are even posting messages, videos on these sites so that they can get attached by more and more users. Researches have shown even in latest 2014 elections that BJP used social media as its most liked option for promotion. The same was hit and liked by masses. Seeing this we can actually see the acceptance of 'Like' on the social media. It has become a measure to actually analyze and capture user interest on social media. It enables one to actually calculate and measure the user liking over certain objects. It gives the clear cut idea of user interest in a particular object and thus calculations can be made accordingly for sharing and promoting information or objects on social media sites [12]. We are trying to seek a model to analyze and mine the influence of 'Like' in social media. To calculate the effect on the object 'Like' mining algorithm is proposed along with the network model for social media. Facebook data is being used to show the influence and effect of the approach. © 2015 IEEE.

Jain R.,Banasthali Vidhyapeeth. | Garg V.,Banasthali Vidhyapeeth. | Yadav D.,Jamia Hamdard University
Biodegradation | Year: 2014

Fungal degradation is emerging as a new powerful tool for the removal of potent neurotoxin pesticide, monocrotophos. Therefore, the present study is aimed at comparative characterization of monocrotophos degrading ability of three different fungal strains. Fungal strains were isolated from local agricultural soil by enrichment culture method, screened by gradient culture and identified as Aspergillus flavus, Fusarium pallidoroseum and Macrophomina sp. Growth kinetics revealed a direct positive influence of monocrotophos on the viability of fungal isolates. Fungal degradation was studied in phosphorus free liquid culture medium supplemented with 150 mg L-1concentration of monocrotophos for a period of 15 days under optimized culture conditions. Degradation of MCP followed first order kinetics with kdegof 0.007, 0.002 and 0.005 day-1and half life (t1/2) of 4.21, 12.64 and 6.32 days for A. flavus, F. pallidoroseum and Macrophomina sp. respectively. To the best of our knowledge, it is the first report signifying the potential of monocrotophos degradation by Fusarium and Macrophomina sp. The results were further confirmed by HPTLC and FTIR which indicates disappearance of monocrotophos by hydrolytic cleavage of vinyl phosphate bond. Degradation of monocrotophos by fungal isolates was accompanied by the release of extracellular alkaline phosphatases, inorganic phosphates and ammonia. The overall comparative analysis followed the order of A. flavus > Macrophomina sp. > F. pallidoroseum. Therefore, it could be concluded from the study that these three different fungal strains could be effectively used as a potential candidate for the removal of monocrotophos from contaminated sites. © 2013 Springer Science+Business Media Dordrecht.

Saluja B.,Banasthali Vidhyapeeth. | Sharma V.,Banasthali Vidhyapeeth.
Soil and Sediment Contamination | Year: 2013

Acidophilic and alkalophilic cadmium-resistant bacterial strains were isolated from the metal-contaminated soil of Panki thermal power plant, Kanpur, India, and two strains, namely SB21 (acidophilic) and SB20 (alkalophilic), were selected as representative strains on the basis of their high resistance to cadmium as compared to other strains. The phylogenetic analysis of the strains using 16s rDNA sequence revealed the strains to be Pseudomonas putida and Comamonas species strain, respectively. Furthermore, to determine the mechanism involved in cadmium resistance, a czc gene was amplified from the strains and sequenced. Homology of the sequences of the two strains, when compared with the available database using a BLASTn search, showed that the 650bp amplicons possess a partial czcA gene sequence. Moreover, the mechanism was confirmed by the results of atomic absorption spectroscopy and transmission electron microscopy studies, which supported the efflux mechanism in the alkalophilic strain SB20, but the acidophilic strain SB21 showed intracellular and periplasmic accumulation of metal in the cells in spite of the presence of the czcA gene, indicating the presence of multiple mechanisms of metal resistance in strain SB21. Further, the strains were characterized functionally for their bioremediation potential in cadmium-contaminated soil under acidic and alkaline conditions by performing an in-situ greenhouse experiment using mungbean plants. A marked increase in agronomical parameters was observed under acidic conditions in the presence of strain SB21. Moreover, the concentration of metal decreased in both plants and soil in the presence of these bioinoculants; however, strain SB21 was found to be a better decontaminator than SB20 and thus can further be employed in bioremediation applications. © 2014 Taylor and Francis Group, LLC.

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