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Kulshrestha V.,Banasthali Vidhyapeeth | Verma S.,Banasthali Vidhyapeeth | Challa C.R.K.,CSE NITTTR
Proceedings of the International Conference on Inventive Computation Technologies, ICICT 2016 | Year: 2017

Cloud computing is a new mechanical and industrial art for hosting the applications and it is flexible, cost-effective, and user-demand platform. It provides the way to share distributed resources and services to all the business or consumer IT sector from desktop and portable PCs over the internet. The advantage of cloud storage is easy access, reduced hardware, low maintenance and installation cost, so each and every organization working with cloud. But it harder to maintain data security, privacy and compliance. Hence it raises serious issues regarding user authentication and data confidentiality. Therefore it is proposed to implement simple data protection framework which performs authentication, verification and encrypt data transfer and thus maintains the data confidentiality. This paper describes about the performance of different security algorithm: AES, RSA and MD5, for ensuring the secure framework.


Dhyani P.,Banasthali Vidhyapeeth | Rishi O.P.,University of Kota
Proceedings - 2015 5th International Conference on Communication Systems and Network Technologies, CSNT 2015 | Year: 2015

Popularity and usability of Social Media in today's scenario is universal. None of us can deny to the fact it is not only the source of communication or connection between friends but also has broaden its horizons to politics, television industry, source to advertisement for companies, education and many more[11][15]. Most of the companies or personalities are maintaining pages on these social media sites to interact with the users of these sites they are even posting messages, videos on these sites so that they can get attached by more and more users. Researches have shown even in latest 2014 elections that BJP used social media as its most liked option for promotion. The same was hit and liked by masses. Seeing this we can actually see the acceptance of 'Like' on the social media. It has become a measure to actually analyze and capture user interest on social media. It enables one to actually calculate and measure the user liking over certain objects. It gives the clear cut idea of user interest in a particular object and thus calculations can be made accordingly for sharing and promoting information or objects on social media sites [12]. We are trying to seek a model to analyze and mine the influence of 'Like' in social media. To calculate the effect on the object 'Like' mining algorithm is proposed along with the network model for social media. Facebook data is being used to show the influence and effect of the approach. © 2015 IEEE.


Gautam R.,Indian Central Arid Zone Research Institute | Singh S.K.,Indian Central Arid Zone Research Institute | Sharma V.,Banasthali Vidhyapeeth
Revista de Biologia Tropical | Year: 2016

The productivity of arid legumes, such as Clusterbean (Cyamopsis tetragonoloba), Cowpea (Vigna unguiculata), Moth bean (Vigna aconitifolia) and Horse gram (Macrotyloma uniflorum), may remain stagnant over decades because of their high susceptibility to root diseases. Besides, there is a limitation on the information about molecular diagnosis and intraspecific genetic variability of root pathogens in arid legumes. To contribute in this field, we assessed a total of 52 isolates from 88 root samples that were found infected with fungal pathogens in Jodhpur, Jaipur and Bikaner Districts of Rajasthan. Diseased roots samples were analyzed following standard microbiological methods for fungus extraction and purification, and for genetic studies. Irrespective of the geographical location from where the diseased samples were collected, all pathogen isolates were clustered in RAPD dendrograms as per their respective genera. Phylogram, based on multiple sequence alignment, revealed that different genera (i.e. Fusarium, Neocosmospora and Syncephalastrum), separated from each other, and species within the same genera, clustered together with their reference sequences with apreciable bootstrap values. Out of 20 representative isolates representing each cluster and all outgroups sequenced, eight were molecularly identified as Neocosmospora vasinfecta, five as Fusarium solani, two as Neocosmospora striata, two as Fusarium acutatum, one as Syncephalastrum monosporum, one as Fusarium oxysporum and one as Fusarium species. The root pathogens of the arid legumes were found neither restricted to a geographical location nor were host specific in nature. Fusarium solani wilt in cowpea and seedling rot in moth bean, F. oxysporum wilt in moth bean, F. acutatum damping off in cowpea and Clusterbean, Fusarium sp. seedling rot in Clusterbean, Neocosmospora striata root rot in cowpea and wilt in Clusterbean and Syncephalastrum monosporum root rot in Clusterbean were molecularly identified as new fungal records as pathogens causing root diseases in arid legumes. © 2016, Universidad de Costa Rica. All rights reserved.


Gautam R.,Indian Central Arid Zone Research Institute | Singh S.K.,Indian Central Arid Zone Research Institute | Sharma V.,Banasthali Vidhyapeeth
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2014

Concurrent heat and moisture stress often favours root diseases caused by Macrophomina phaseolina in arid legumes. Molecular analysis revealed 92 % variation within M. phaseolina populations as compared to 8 % among populations. The first three principle coordinates of PCA analysis accounted for a 69.61 % of total variance and Eigen vectors revealed 22.89 % of total variability. The mean values of all the four populations together for Nei's gene diversity (h) was 0.1990 and Shannon's information index (i) was 0.3113. The result showed that the genetic diversity of M. phaseolina isolates of population 2 (cowpea) was richest among all the four populations. Analysis of molecular variance indicated that main proportion of genetic variation was within the host than among different hosts. Out of 13 representative isolates seven were molecularly identified as Rhizoctonia bataticola and six as M. phaseolina upon sequencing of 5.8S RNA region. Besides length variation in ITS-1, 5.8S rRNA gene, ITS-2 and total length, the authors report insertion/deletions at a number of places in 13 isolates sequenced. This study underlines that M. phaseolina distribution is independent of host and/or geography and validates the utility of ITS rDNA region as a reliable indicator of phylogenetic interrelationships as an additional approach for identification of the genus Macrophomina and assessing its genetic diversity. © 2013 The National Academy of Sciences, India.


Objective: The present study aimed the characterization and purification of acid phosphatases from monocrotophos (MCP) hydrolyzing Aspergillus niger ITCC 7782.10 isolated from local agricultural field. Material and Methods: Intracellular phosphatases from MCP hydrolyzing Aspergillus niger ITCC 7782.10 were purified by four step purification procedure which were further characterized. Results: The study demonstrated the presence of two intracellular phosphatases of varying molecular weights of 33 and 67 kDa which were purified to 126 and 76 fold respectively by four step purification strategy. Both the phosphatases showed similar pattern for pH, temperature and metal ion however, the activity of P33 was found to be higher than that of P67. The phosphatases were found to be most active at acidic pH and were found to be stable till 80°C demonstrating them to be acid phosphatases and thermos table. Km and Vmax values of P33 was found to be 0.28 mM and 1.21 Umg-1protein respectively whereas Km of P67 was found to be 0.72 mM and Vmax value was 1.85 Umg-1protein. This indicated that P33 had more affinity towards para nitro phenol phosphate (pNPP) as a substrate than P67. Conclusion: This is the first report indicating the presence of two different intracellularly thermostable acid phosphatases within the same MCP hydrolyzing fungal strain Aspergillus niger ITCC 7782.10. Therefore, these acid phosphatases could be effectively used for the bioremediation of MCP contaminated sites.


Yadav P.,Jiwaji University | Koul K.K.,Jiwaji University | Srivastava N.,Banasthali Vidhyapeeth | Mendki M.J.,Defense Research & Development Establishment | Bhagyawant S.S.,Jiwaji University
Plant Biosystems | Year: 2016

Twenty chickpea accessions belonging to ten different countries of the world have been subjected to phylogeny and length variations from nuclear ribosomal DNA (nr DNA). ITS1–5.8S–ITS2 regions of Cicer accessions were used for amplification in two sets, each comprising a reverse and forward ITS primers. Lengths of ITS-1 of C. arietinum and C. reticulatum ranged from 340 to 350 bp whereas that of ITS-2 from 400 to 410 bp. In all the 20 accessions investigated, GC content in ITS-1 ranged from 40 to 55% and in ITS-2 from 42 to 55%. Sequencing of polymerase chain reaction product from ITS-1 showed variability in 278 and 290 bp due to adenine and guanine nucleotide base pairs. BLAST search for ITS-1 region revealed highest homology (99%) with four strains of C. arietinum accessions. Whereas, ITS2 showed 100% homology with C. arietinum and 99% homology with that of C. echinospermum. © 2016 Società Botanica Italiana


Jain R.,Banasthali Vidhyapeeth | Garg V.,Banasthali Vidhyapeeth | Yadav D.,Jamia Hamdard University
Biodegradation | Year: 2014

Fungal degradation is emerging as a new powerful tool for the removal of potent neurotoxin pesticide, monocrotophos. Therefore, the present study is aimed at comparative characterization of monocrotophos degrading ability of three different fungal strains. Fungal strains were isolated from local agricultural soil by enrichment culture method, screened by gradient culture and identified as Aspergillus flavus, Fusarium pallidoroseum and Macrophomina sp. Growth kinetics revealed a direct positive influence of monocrotophos on the viability of fungal isolates. Fungal degradation was studied in phosphorus free liquid culture medium supplemented with 150 mg L-1concentration of monocrotophos for a period of 15 days under optimized culture conditions. Degradation of MCP followed first order kinetics with kdegof 0.007, 0.002 and 0.005 day-1and half life (t1/2) of 4.21, 12.64 and 6.32 days for A. flavus, F. pallidoroseum and Macrophomina sp. respectively. To the best of our knowledge, it is the first report signifying the potential of monocrotophos degradation by Fusarium and Macrophomina sp. The results were further confirmed by HPTLC and FTIR which indicates disappearance of monocrotophos by hydrolytic cleavage of vinyl phosphate bond. Degradation of monocrotophos by fungal isolates was accompanied by the release of extracellular alkaline phosphatases, inorganic phosphates and ammonia. The overall comparative analysis followed the order of A. flavus > Macrophomina sp. > F. pallidoroseum. Therefore, it could be concluded from the study that these three different fungal strains could be effectively used as a potential candidate for the removal of monocrotophos from contaminated sites. © 2013 Springer Science+Business Media Dordrecht.


Choudhary N.K.,Banasthali Vidhyapeeth.
Journal of complementary & integrative medicine | Year: 2012

This study was designed to examine the antioxidant defense by chloroform extract of Calotropis gigantea on streptozotocin-(40mg/kg, intraperitonial, single-injection) induced diabetes in wistar albino rats. The extract significantly (P < .05) decreased the pancreatic thiobarbituric acid-reactive substances (TBARS) levels and significantly (P < .05) increased the superoxide dismutase, catalase, and glutathione levels as compared to above levels in pancreatic tissue of pathogenic diabetic rats. The results of test drug were comparable to Glibenclamide (5mg/kg, daily), a standard antihyperglycemic agent. The study concludes that Calotropis gigantea enhances the antioxidant defense against reactive oxygen species produced under hyperglycemic condition and this protects β-cells against loss, and exhibit antidiabetic property.


Saluja B.,Banasthali Vidhyapeeth | Sharma V.,Banasthali Vidhyapeeth
Soil and Sediment Contamination | Year: 2013

Acidophilic and alkalophilic cadmium-resistant bacterial strains were isolated from the metal-contaminated soil of Panki thermal power plant, Kanpur, India, and two strains, namely SB21 (acidophilic) and SB20 (alkalophilic), were selected as representative strains on the basis of their high resistance to cadmium as compared to other strains. The phylogenetic analysis of the strains using 16s rDNA sequence revealed the strains to be Pseudomonas putida and Comamonas species strain, respectively. Furthermore, to determine the mechanism involved in cadmium resistance, a czc gene was amplified from the strains and sequenced. Homology of the sequences of the two strains, when compared with the available database using a BLASTn search, showed that the 650bp amplicons possess a partial czcA gene sequence. Moreover, the mechanism was confirmed by the results of atomic absorption spectroscopy and transmission electron microscopy studies, which supported the efflux mechanism in the alkalophilic strain SB20, but the acidophilic strain SB21 showed intracellular and periplasmic accumulation of metal in the cells in spite of the presence of the czcA gene, indicating the presence of multiple mechanisms of metal resistance in strain SB21. Further, the strains were characterized functionally for their bioremediation potential in cadmium-contaminated soil under acidic and alkaline conditions by performing an in-situ greenhouse experiment using mungbean plants. A marked increase in agronomical parameters was observed under acidic conditions in the presence of strain SB21. Moreover, the concentration of metal decreased in both plants and soil in the presence of these bioinoculants; however, strain SB21 was found to be a better decontaminator than SB20 and thus can further be employed in bioremediation applications. © 2014 Taylor and Francis Group, LLC.


PubMed | Banasthali Vidhyapeeth
Type: Comparative Study | Journal: Biodegradation | Year: 2014

Fungal degradation is emerging as a new powerful tool for the removal of potent neurotoxin pesticide, monocrotophos. Therefore, the present study is aimed at comparative characterization of monocrotophos degrading ability of three different fungal strains. Fungal strains were isolated from local agricultural soil by enrichment culture method, screened by gradient culture and identified as Aspergillus flavus, Fusarium pallidoroseum and Macrophomina sp. Growth kinetics revealed a direct positive influence of monocrotophos on the viability of fungal isolates. Fungal degradation was studied in phosphorus free liquid culture medium supplemented with 150 mg L(-1) concentration of monocrotophos for a period of 15 days under optimized culture conditions. Degradation of MCP followed first order kinetics with kdeg of 0.007, 0.002 and 0.005 day(-1) and half life (t1/2) of 4.21, 12.64 and 6.32 days for A. flavus, F. pallidoroseum and Macrophomina sp. respectively. To the best of our knowledge, it is the first report signifying the potential of monocrotophos degradation by Fusarium and Macrophomina sp. The results were further confirmed by HPTLC and FTIR which indicates disappearance of monocrotophos by hydrolytic cleavage of vinyl phosphate bond. Degradation of monocrotophos by fungal isolates was accompanied by the release of extracellular alkaline phosphatases, inorganic phosphates and ammonia. The overall comparative analysis followed the order of A. flavus > Macrophomina sp. > F. pallidoroseum. Therefore, it could be concluded from the study that these three different fungal strains could be effectively used as a potential candidate for the removal of monocrotophos from contaminated sites.

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