Balaji Institute of Pharmaceutical science

Warangal, India

Balaji Institute of Pharmaceutical science

Warangal, India
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Gopal N.,Balaji Institute of Pharmaceutical science | Jayakar B.,Salem College
Global Journal of Pharmacology | Year: 2012

A new, simple and precise HPLC method was developed for the determination of Lafutadine and Domperidone in capsule dosage form. Reversed phase chromatography was performed at Phenomenex, C18, 5μ particle size, 250 × 4.6 mm column as stationary phase and the mobile phase consist of 0.02 M Potassium dihydrogen phosphate: Acetonitrile: Methanol in a ratio of 50:35:15 v/v/v at the flow rate of 1 ml/min. All the analytes were quantified at 285 nm. Statistical validation of the method included determination of linearity, accuracy and precision. The method is rapid and reproducible and could be used for direct determination of Lafutadine and Domperidone in different dosage forms. © IDOSI Publications, 2012.


Meenakshi K.,Balaji Institute of Pharmaceutical science | Gopal N.,Balaji Institute of Pharmaceutical science | Sarangapani M.,Balaji Institute of Pharmaceutical science
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: Synthesis and antimicrobial evaluation of some novel Schiff and Mannich bases of Isatin. Methods: A series of novel Schiff bases of Isatin (V) and (X) were synthesized by refluxing Isatin with p-amino ethyl benzoate (IV) and 4-(4!-amino phenyl)-6-substituted phenyl pyrimidine-2-thiol (IX). Mannich bases of ethyl-4-(2-oxindolin-3-ylidene amino) benzoate (VI) were synthesized by using various aromatic secondary amines. The chemical structures of synthesized compounds were confirmed by IR, 1HNMR, Mass and elemental analysis. These compounds were also screened for their in vitro antibacterial and antifungal activities. Results: The results of antibacterial and antifungal activities showed that some of the synthesized compounds were exhibited promising antimicrobial activities. Conclusion: All the newly synthesized compounds were screened for antimicrobial activities by turbidity method using Ampicillin and Clotrimazole as standard against gram positive, gram negative bacteria and fungi.


Siddartha Kumar P.,Balaji Institute of Pharmaceutical science | Junapudi S.,Anurag Pharmacy College | Gurrala S.,Gland institute of Pharmaceutical science
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2011

The conventional methodology was adopted to synthesize the titled compounds. The synthesis of titled compounds from starting compound i.e 2-amino-3-carboxamido-4,5,6,7 tetramethylene thiophene (SRP-2) was prepared from cyanoacetamide (SRP-1) by condensation with cyclohexanone in the presence elemental sulphur and a basic catalyst diethyl amine in ethanol to form (SRP-2). A set of thirteen azomethine derivatives (Schiff bases) were synthesized by reacting 2-amino-3-carboxamido-4,5,6,7-tetramethylene thiophene (SRP-2) in isopropyl alcohol with various substituted aromatic aldehydes involving glacial acetic acid as a catalyst. The title compounds (SRP-2a-m) were screened for their antibacterial activity against two Gram-positive bacteria i.e. Staphylococcus aureus & Bacillus subtilus and two Gram-negative bacteria i.e. Escherichia coli & Klebsiella pneumoniae using ampicillin as standard, each at a concentration of 50μg/0.1ml, adapting agar diffusion method. The compounds were also screened for their antifungal activity against two pathogenic fungi i.e. Candida albicans and Aspergillus niger using miconazole nitrate as standard at a concentration of 50μg/0.1ml, adapting agar diffusion method.


Ananthakumar R.,Balaji Institute of Pharmacy | Raja N.,Balaji institute of Pharmaceutical science | Mohan P.,Balaji institute of Pharmaceutical science | Ruckmani K.,Anna University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Objective: The main objective of this study is to investigate the effect of formulation variables on drug release and floating properties of the delivery system. Hydroxy propyl methyl cellulose (HPMC) of different viscosity grades and Carbopol 934P (CP934P) were used in formulating the Gastric Floating Drug Delivery System (GFDDS) employing 2× 3 full factorial design. The formulation was optimized on the basis of in vitro buoyancy and in vitro release in simulated fed state gastric fluid (pH 1.2). Effect of various release modifiers was studied to ensure the Floating tablet as Hydro dynamically Balanced System over a prolonged period. Methods: The tablets were prepared by the wet granulation method, using hydrophilic matrix polymers HPMC K4M, HPMC K100LV, CP934P, sodium bicarbonate. Tablets were physically characterized and evaluated for in vitro release characteristics for 12 h in 0.1mol/l HCl at 37°C. The in vitro drug release, buoyancy lag-time, swelling index were evaluated. It was found that in tablets prepared with HPMC, the presence of CP934P had significant impact on the release and floating properties of the delivery system. In tablets prepared with low viscosity polymer (HPMC K4M), incorporation of CP934P was found to compromise the floating capacity of GFDDS and release rate of metformin hydrochloride. Results and conclusion: Tablets are prepared with HPMC K4M and CP934P which gives the best in vitro percentage drug release and used as the optimized formulation. The viscosities of HPMC significantly affect the drug release rate, buoyancy lag-time, swelling characteristics of the tablets. By fitting the data in to zero order, first order, Higuchi and Korsemeyer-Peppa's model it concludes that the release followed zero order release, where as the correlation co-efficient (R2 value) was higher for zero order release. The release mechanism follows Higuchi model, Korsemeyer- Peppa's model and non-fickian diffusion.


Aarelly K.,Balaji Institute of Pharmaceutical science | Bheemanapally K.,Balaji Institute of Pharmaceutical science | Thimmaraju M.K.,Balaji Institute of Pharmaceutical science | Nerella R.,Balaji Institute of Pharmaceutical science
Der Pharmacia Lettre | Year: 2013

New, simple, sensitive, cost effective and reproducible UV-spectrophotometric method was developed and validated for the estimation of naftopidil in bulk and tablet formulations. Naftopidil was estimated at 280 nm using acetonitrile-hydrochloric acid (pH 1.2, 100 mM) (25:75, ν/ν) as solvent system. This method was tested and validated for various parameters as per USP requirements and ICH guidelines.


Aarelly K.,Balaji Institute of Pharmaceutical science | Thimmaraju M.K.,Balaji Institute of Pharmaceutical science | Nerella R.,Balaji Institute of Pharmaceutical science | Allabotharam S.,Balaji Institute of Pharmaceutical science
Journal of Chemical and Pharmaceutical Research | Year: 2012

A simple, sensitive, precise and specific reverse phase high performance liquid chromatographic method was developed and validated for the determination of Asenapine in bulk and tablet dosage forms. It was found that the excipient in the tablet dosage forms does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography on Shimadzu HPLC, 10-At detector with hypersil ODS C18 Column 250 X 4.6 mm (particle size of 5μ) and constant flow pump. Rheodyne injector with 20μl loop with a mobile phase composed pure methanol at flow rate 1.0 ml/min. The detection was monitored at 270nm. The calibration curve for Tamsulosin was linear from 2-10mg/ml. The interday and intraday precision was found to be within limits. The proposed method has adequate sensitivity, reproducibility and specificity for the determination of Asenapine in bulk and its tablet dosage forms. LOD and LOQ for Asenapine were found to be 0.4329 and 0.1311.Accuracy (recoveries: 98.07-101.28%) and reproducibility were found to satisfactory.


Krishnakumar E.,Balaji Institute of Pharmaceutical science
Avicenna Journal of Medical Biotechnology | Year: 2010

Adjuvant induced arthritis is a chronic crippling, skeleton-muscular disorder having nearest approximation to human rheumatoid arthritis for which there is currently no medicine available effecting a permanent cure. Even modern drugs used for the amelioration of the symptoms, offer only temporary relief and also produce severe side effects. In the indigenous system of medicine, wood of Premna serratifolia Linn., is reported to be useful in the treatment of arthritis. It is a large shrub, distributed throughout Asia, used against a wide variety of diseases. However, no systematic study has been reported regarding its anti-arthritic activity. This work was aimed at the scientific validation of the ethno-pharmacological claim about its anti-arthritic property. In the present study, anti-arthritic activity of ethanol extract of Premna serratifolia Linn., wood is done by Freund's adjuvant induced arthritis model. Loss in body weight during arthritis condition was corrected on treatment with ethanol extract and standard drug, indomethacin. Biochemical parameters such as hemoglobin content, total WBC, RBC, erythrocyte and sedimentation rate were also estimated. The ethanol extract at the dose of 300 mg/kg body weight inhibited the rat paw edema by 68.32% which is comparable with standard drug indomethacin 74.87% inhibition of rat paw edema after 21 days. The results of the current investigation concluded, ethanol extract of Premna serratifolia Linn., wood possess a significant anti-arthritic activity against adjuvant induced arthritis and justifying its therapeutic role in arthritic condition. The observed antiarthritic activity may be due to the presence of phytoconstituents such as irridiod glycosides, alkaloids, phenolic compounds and flavonoids. Copyright © 2010, Avicenna Journal of Medical Biotechnology. All rights reserved.


Srikanth L.,Prasad Institute of Pharmaceutical science | Raghunandan N.,Balaji Institute of Pharmaceutical science
Der Pharma Chemica | Year: 2011

Benzimidazoles display a broad spectrum of potential pharmacological activities and are present in a number of pharmacologically active molecules such as Albendazole/ Mebendazole/ Thiabendazole (antihelmentic), Omeprazole (anti-ulcer). These compounds carrying different substituents in the benzimidazole structure are associated with a wide range of biological activities. Changes in their structure have offered a high degree of diversity that has proven useful for the development of new therapeutic agents having improved potency and lesser toxicity. In this context, the recently synthesized 2-Substituted benzimidazole derivatives possessing important pharmacological activities have been highlighted.


Raghunandan N.,Balaji Institute of Pharmaceutical science
Asian Journal of Pharmaceutical and Clinical Research | Year: 2016

Objective: The objective of the study was to evaluate the antihyperglycemic activity and in vivo antioxidant effect of Artocarpus hirsutus seeds in both normal and diabetic rats. Methods: Male Wistar rats weighing about 180-250 g were divided into six groups, of six rats each. Diabetes was induced by giving streptozotocin (30-50 mg/kg) intraperitoneally. Rats, which showed blood glucose levels ≥250 mg/dl, were selected for the study. Metformin (50 mg/kg) was used as a standard oral hypoglycemic agent. Oral glucose tolerance test was performed in all groups of rats. In the estimation of in vivo antioxidant activity, the levels of liver enzymes superoxide dismutase (SOD), lipid peroxidation, and CAT (catalase) were measured using standard methods. Results: The ethyl acetate seed extract of A. hirsutus at different doses was selected and administered orally. The blood glucose levels were estimated by the glucose oxidase method, and insulin levels were measured by chemiluminescence assay method. Antihyperglycemic activity of the test drug in diabetic rats showed a significant reduction in blood glucose levels (p<0.001) at 2, 4, 6, and 8 hrs, respectively, as compared to diabetic groups. The antioxidant enzymes SOD and CAT levels were significantly raised, whereas malondialdehyde-thiobarbituric acid residue substances levels have decreased (p<0.001). Conclusion: The results suggested that A. hirsutus seed extract showed a potential antidiabetic activity and antioxidant effect justifying the use of the drug for the treatment of diabetes mellitus and its associated oxidative damage. © 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.


Lingala S.,Acharya Nagarjuna University | Nerella R.,Balaji Institute of Pharmaceutical science | Sambasiva Rao K.R.S.,Acharya Nagarjuna University
Der Pharma Chemica | Year: 2011

New benzimidazole derivatives containing 4-chloropyridine-2-carbonyl and N-methyl picolinamide moieties in one side chain at 1H position of benzimidazoles (2-((benzylthio)methyl)-1H-benzo[d]imidazol-1-yl)(4-chloropyridin-2-yl)methanones(3) have been synthesized as depicted in scheme-1. The intermediates and final compounds were purified and their chemical structures have been confirmed by IR, 1H NMR, and Mass spectral data. All the derivatives were examined for their anthelmintic activity against Indian adult earthworms (pheretima posthuma) at various concentrations (0.2% and 0.5%), antibacterial activity against B.subtilis, B.cereus, S.epidermidis, S.typhi, P.aeruginosa and K.pneumoniae and antifungal activity against A.flavus, F.oxysporium and P.notatum at a concentration of 2mg/ml. Most of the compounds tested have shown promising activities when compared with the standard drugs.

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