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Ozbek E.,Bezm i Alem Valide Sultan Vakif Gureba Research and Education Hospital | Tasci A.I.,Bakirkoy Research and Education Hospital | Ilbey Y.O.,Bezm i Alem Valide Sultan Vakif Gureba Research and Education Hospital | Simsek A.,Bezm i Alem Valide Sultan Vakif Gureba Research and Education Hospital | And 2 more authors.
International Journal of Andrology | Year: 2010

Erectile dysfunction (ED) is a major public health problem that seriously affects the quality of life of patients and their partners and its prevalence increases significantly with ageing. In this study, we tested the hypothesis that age-associated decrease in penile endothelial (eNOS) and neuronal nitric oxide synthase (nNOS) activity in aged rats may be increased by regular exercise. A total of 28 young (4 m) and aged (24 m) male rats were divided into four equal groups: group 1 - young control; group 2 - young trained; group 3 - old control and group 4 - old trained group. Groups 2 and 4 rats were trained to swim for 30 min a day and 5 days a week, which lasted 8 weeks. At the end of 8 weeks, rats were sacrificed and penile tissues evaluated for eNOS and nNOS activities. eNOS and nNOS activities were evaluated by immunohistochemistry in paraffinized penile tissues and results assessed semiquantitatively. Results also were compared with healthy age-matched and adult (4 m) controls. Serum level of testosterone (T) was determined using ELISA kits (Beckman Coulter, Fullerton, CA, USA). In penile tissues of aged control rats, eNOS and nNOS staining were weakly positive; however in trained groups, eNOS and nNOS immunoreactivity were increased. In young control group, eNOS and nNOS activities were more intense than aged control. eNOS and nNOS activities were higher in adult trained group than control. Serum T concentrations were significantly higher in young and aged trained group than in control groups. We can suggest that regular exercise upregulates eNOS and nNOS expressions in the aged and young rat penis. Regular exercise may improve penile erection by increasing penile neurotransmitter in both young and aged rats. © 2010 European Academy of Andrology. Source

Simsek A.,Bezm i Alem Valide Sultan Vakif Gureba Research and Education Hospital | Ozbek E.,Bezm i Alem Valide Sultan Vakif Gureba Research and Education Hospital | Ilbey Y.O.,Bezm i Alem Valide Sultan Vakif Gureba Research and Education Hospital | Cekmen M.,Kocaeli University | And 2 more authors.
Andrologia | Year: 2012

The aim of this study was to investigate p38-mitogene-activated protein kinase (p38-MAPK), nuclear factor-kappa B (p65-NF-kB) and inducible nitric oxide synthase (iNOS) expression in an experimental model of varicocele in the rat testis. Male Wistar albino rats (n=18) were divided into three equal groups: control group, sham operated group and left varicocele-induced group. Malondialdehyde (MDA), nitric oxide (NO) and reduced glutathione (GSH) levels were biochemically assessed, and the p38-MAPK and NF-kB activity, and iNOS expression were immunohistochemically studied in the right and left testicles of rats from each group. The GSH levels were significantly decreased, whereas the level of MDA and NO was significantly increased in the testicular tissues of rats in varicocele group compared with those of the control and sham groups. There was a marked staining for iNOS, p38-MAPK and p65-NF-kB expression in rats of varicocele group compared with the sham group. There was no positive staining in rats of control group. There were significant differences in biochemical, histological and immunohistochemical studies, but no significant differences were noted between other groups. p38-MAPK and p65-NF-kB activation, and iNOS expression have a significant role in varicocele-induced testicular dysfunction. © 2011 Blackwell Verlag GmbH. Source

Ilbey Y.O.,Bezm i Alem Valide Sultan Vakif Gureba Research and Education Hospital | Ilbey Y.O.,Istanbul Vakif Gureba Research and Education Hospital | Ozbek E.,Bezm i Alem Valide Sultan Vakif Gureba Research and Education Hospital | Ozbek E.,Istanbul Vakif Gureba Research and Education Hospital | And 6 more authors.
Archivio Italiano di Urologia e Andrologia | Year: 2010

The aim of this study was to evaluate the role of the inducible nitric oxide synthase (iNOS), selective nuclear factor-kB (NF-kB) and p38-mitogene-activated protein kinase (p38-MAPK) on oxalate-induced crystal deposition in renal tubules. The rats were divided into three groups; group 1; control group, group 2; ethylene glycol (EG) group, group 3; EG + pyrrolidine dithiocarbamate (PDTC) group. Rats were sacrified on 7, 15 and 45th days. The iNOS expression, p65/NF-kB and p38/MAPK activity, and oxidative stress markers were evaluated in the kidney. Crystal depositions were evident on day 7, mild and severe cyrystallization were observed on day 15 and 45 in EG group, respectively. There was limited or no cyrstal formation in the EG + PDTC group. While EG stimulates iNOS, p65/NF-kB and p38/MAPK activity in renal tubules, PDTC inhibited it. PDTC prevents crystal depositions in renal tubules by reducing oxidative stress, iNOS, NF-kB, and p38-MAPK expression. Source

Ozge A.,Mersin University | Selekler M.,Atasehir Memorial Hospital | Ozturk M.,Bakirkoy Research and Education Hospital | Baykan B.,Istanbul University | And 10 more authors.
Geriatrics and Gerontology International | Year: 2015

Aim: Chronic migraine is a growing and disabling subtype of migraine with different risk factors and clinical features, even in older adults. We sought to define and differentiate clinical features of chronic migraine in older adults. We also aimed to compare major clinical features of chronic migraine in older adults with those in younger people of both sexes. Methods: We used electronic dataset (Turkish Headache Database) from 13 tertiary headache centers in Turkey. Electronic dataset included detailed headache-defining features according to ICHD-II criteria based on face-to-face interviews and examination by a headache specialist. Using statistical methods, clinical variables of chronic migraine in older adults were compared with those of younger adults. We included 915 patients with chronic migraine (mean age 43.80±13.95 years); 83.3% were females. In total, 301 patients (32.9%) with chronic migraine aged >50 years were compared with 614 patients aged <50 years. Results: There was no significant change in men with increasing age. However, duration of headache history, severity of attacks, previous histories of motion sickness and positive family history of headaches were significantly different in women with increasing age. Further sex-related differences have been shown in parameters such as attack duration, quality and associated nausea. Conclusion: Chronic migraine is an infrequent type of migraine and shows age-related changes in some phenotypic characteristics, such as severity of attacks, especially in women aged older than 50 years. Furthermore, positive family history of headaches and history of motion sickness increase the likelihood of developing chronic migraine in older women, indicating involvement of some gender-related, but as-yet unknown, genetic factors. Geriatr © 2014 Japan Geriatrics Society. Source

Tugcu B.,Bakirkoy Research and Education Hospital | Helvacioglu F.,Maltepe University | Yuzbasioglu E.,Istanbul Science University | Gurez C.,Canakkale Government Hospital | Yigit U.,Bakirkoy Research and Education Hospital
Japanese Journal of Ophthalmology | Year: 2013

Purpose: To reduce postoperative scar formation and to improve duction using amniotic membrane (AM) in strabismus reoperations. Methods: A prospective study of interventional case series comprised of 14 patients with restrictive strabismus. Objective clinical findings (visual acuity, angle of deviations and degree of duction deficits) were recorded in both the pre- and post-operative periods. Strabismus surgery included the excision of adhesions and scar tissue, repositioning of extraocular muscles (according to the degree of deviations) and placement of two sheets, one between muscle and tenon and the other between muscle and sclera. Conjunctival recession with covering of the bare sclera using AM was also performed. Results: The mean preoperative deviation of the patients was 34.3 prism diopters (PD) and mean post-operative deviation was 4.6 PD. The mean pre-operative duction deficit of the patients was 1.7; mean post-operative duction deficit was 0.2. Postoperatively all patients improved in relation to deviation and duction. Less than 8 PD deviations with no duction deficits were achieved in 12 (86 %) of the patients. Conclusions: Amniotic membrane placement around the extraocular muscle improves the duction and decreases the residual angle of deviations by inhibiting postoperative scar formation. © 2012 Japanese Ophthalmological Society. Source

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