Srivastava P.,Indian Institute of Toxicology Research |
Yadav R.S.,Indian Institute of Toxicology Research |
Yadav R.S.,Dr Hari Singh Gour University |
Chandravanshi L.P.,Indian Institute of Toxicology Research |
And 6 more authors.
Toxicology and Applied Pharmacology | Year: 2014
Earlier, we found that arsenic induced cholinergic deficits in rat brain could be protected by curcumin. In continuation to this, the present study is focused to unravel the molecular mechanisms associated with the protective efficacy of curcumin in arsenic induced cholinergic deficits. Exposure to arsenic (20. mg/kg body weight, p.o) for 28. days in rats resulted to decrease the expression of CHRM2 receptor gene associated with mitochondrial dysfunctions as evident by decrease in the mitochondrial membrane potential, activity of mitochondrial complexes and enhanced apoptosis both in the frontal cortex and hippocampus in comparison to controls. The ultrastructural images of arsenic exposed rats, assessed by transmission electron microscope, exhibited loss of myelin sheath and distorted cristae in the mitochondria both in the frontal cortex and hippocampus as compared to controls. Simultaneous treatment with arsenic (20. mg/kg body weight, p.o) and curcumin (100. mg/kg body weight, p.o) for 28. days in rats was found to protect arsenic induced changes in the mitochondrial membrane potential and activity of mitochondrial complexes both in frontal cortex and hippocampus. Alterations in the expression of pro- and anti-apoptotic proteins and ultrastructural damage in the frontal cortex and hippocampus following arsenic exposure were also protected in rats simultaneously treated with arsenic and curcumin. The data of the present study reveal that curcumin could protect arsenic induced cholinergic deficits by modulating the expression of pro- and anti-apoptotic proteins in the brain. More interestingly, arsenic induced functional and ultrastructural changes in the brain mitochondria were also protected by curcumin. © 2014 Elsevier Inc.
Srivastava S.,Eras Lucknow Medical College |
Singh K.,Babu Banarasi Das University
Current Research in Nutrition and Food Science | Year: 2016
Beetroot, botanically called “Beta Vulgaris” is taproot portion of the beet plant. Beetroot are rich in many valuable active compounds and nutrients. Therefore, beetroot ingestion can be considered a factor in disease prevention. The present study aimed at the formulation of pickle product. The beetroot pickle were prepared by three different standardized methods in three different samples of Beetroot Pickle A (100% beetroot salty pickle prepared by raw beetroot), Beetroot Pickle B (100% beetroot sweet pickle prepared by steamed beetroot) and Beetroot Pickle -C (100% beetroot salty pickle prepared by boiled beetroot). The developed pickles were sensory evaluated by judges where using Nine-Point hedonic scale. The result indicates that Pickle-C were most acceptable than other two preparations.The fresh beetroots and most acceptable beetroot pickles sample were chemically analysed by standard AOAC,2000 methods for Betaine, Vitamin C, Iron, Folate, Choline and Calcium. The results showed that all Nutritional profiles were decreasing from fresh beetroots to beetroot pickles. But it was found that Vitamin C content was increased in Beetroot Pickles than fresh beetroots. © 2016 Enviro Research Publishers. All rights reserved.
Gupta J.,Babu Banarasi Das University |
Kulshreshtha M.,Babu Banarasi Das University
International Journal of Nutrition, Pharmacology, Neurological Diseases | Year: 2017
Dementia or memory loss is the hallmark symptom of Alzheimer's disease (AD). The exploration of AD helps to identify the problems involved in neural structures such as the default network and role of genetic factors. Episodic memory (EM) directly deals with the memory system that allows an individual to consciously retrieve an old experience or an episode of life. EM plays an important role in AD. The World Health Organization predicts that, by 2025, about 75% of the estimated 1.2 billion people aged 60 years and older will reside in developing countries. It is estimated that the number of people living with dementia will almost double every 20 years to 42.3 million in 2020 and 81.1 million in 2040. In India, there are lesser number of good quality scientific studies/researches that deal with the real trend of the disease and determine the mechanism involved, risk factors, investigations on dementia, decline in awareness, and inadequate availability of social benefit. India is a country with varied cultures, and, therefore, conducting a genetic epidemiological study here has greater advantage. The goal of this review is to provide knowledge and increase the awareness of dementia associated with AD, as well as to understand the mechanism involved and its treatment, which can be useful for researchers or scientists in the near future. © 2017 International Journal of Nutrition, Pharmacology, Neurological Diseases.
Meenu J.,Northern India Engineering College |
Sharma S.,Guru Jambheshwar University of Science and Technology |
Kumar M.,Babu Banarasi Das University
International Journal of Green Pharmacy | Year: 2011
Ethnomedicinally the seeds of Vigna mungo are used as cooling astringent, diet during fever, poultice for abscesses, soap alternative. The increased oxidative stress plays an important role in the etiology and pathogenesis of diabetes mellitus and its various complications. This study was designed to examine the effect of hydroalcoholic extract (VME) of V. mungo seeds on STZ-diabetic rats by measuring glycemia, lipid profile, and lipid peroxidation parameters (MDA, PCO, and GSH) and antioxidant enzymes activities (SOD, CAT, and GPx). The levels of glucose, TG, TC, MDA, and PCO were increased significantly whereas the levels of serum insulin, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and Glutathione peroxidise (GPx) were decreased in STZ induced diabetic rats. Administration of VME (200 mg/kg bw and 400 mg/kg bw p.o.) to diabetic rats for 28 days showed a significant decrease in serum glucose, TG, TC, MDA, and PCO. In addition, we also summarize here that the levels of serum insulin, superoxide dismutase, catalase, GPx, and reduced glutathione (GSH) were increased in VME treated diabetic rats. The antioxidant and antihyperglycemic effect of VME was compared with glibenclamide, a well-known antioxidant and antihyperglycemic drug. The findings in this study suggest that the VME possesses a significant favourable effect on antioxidant defense system in addition to its antidiabetic effect. Nonetheless, this study provides evidence that could help explain how the traditional use of V. mungo has been successful in the treatment of various disorders in humans.
Ratnasekhar C.,Academy of Scientific and Innovative Research |
Sonane M.,Indian Institute of Toxicology Research |
Sonane M.,Babu Banarasi Das University |
Satish A.,Indian Institute of Toxicology Research |
Mudiam M.K.R.,Academy of Scientific and Innovative Research
Nanotoxicology | Year: 2015
The increasing use of nanotechnology in our daily life can have many unintended effects and pose adverse impact on human health, environment and ecosystems. Wider application of engineered nanoparticles, especially TiO2 nanoparticles (TiO2 NP) necessitates the understanding of toxicity and mechanism of action. Metabolomics provides a unique opportunity to find out biomarkers of nanoparticles exposure, which leads to the identification of cellular pathways and their biological mechanisms. Gas chromatography mass spectrometry (GC–MS)-based metabolomics approach was used in the present study to understand the toxicity of sub-lethal concentrations (7.7 and 38.5 µg/ml) of TiO2 NP (<25 nm) in well-known, soil nematode Caenorhabditis elegans (C. elegans). Multivariate pattern recognition analysis reflected the perturbations in the metabolism (amino acids, organic acids, sugars) of C. elegans on exposure to TiO2 NP. The biological pathways affected due to the exposure of TiO2 NP were identified, among them mainly affected pathways are tricarboxylic acid (TCA) cycle, arachidonic acid metabolism and glyoxalate dicarobxylate metabolism. The manifestation of differential metabolic profile in organism exposed to TiO2 (NP or bulk particle) was witnessed as an effect on reproduction. The present study demonstrates that metabolomics can be employed as a tool to understand the potential toxicity of nanoparticles in terms of organism–environment interactions as well as in assessing the organism function at the molecular level. © 2015 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted
Kaur K.,Babu Banarasi Das University |
Gupta R.,Babu Banarasi Das University |
Saraf S.A.,Babasaheb Bhimrao Ambedkar University |
Saraf S.K.,Babu Banarasi Das Northern India Institute of Technology
Comprehensive Reviews in Food Science and Food Safety | Year: 2014
The importance of zinc was 1st reported for Aspergillus niger. It took over 75 y to realize that zinc is also an essential trace element for rats, and an additional 30 y went by before it was recognized that this was also true for humans. The adult body contains about 2 to 3 g of zinc. Zinc is found in organs, tissues, bones, fluids, and cells. It is essential for many physiological functions and plays a significant role in a number of enzyme actions in the living systems. Bioinformatics estimates report that 10% of the human proteome contains zinc-binding sites. Based on its role in such a plethora of cellular components, zinc has diverse biological functions from enzymatic catalysis to playing a crucial role in cellular neuronal systems. Thus, based on the various published studies and reports, it is pertinent to state that zinc is one of the most important essential trace metals in human nutrition and lifestyle. Its deficiency may severely affect the homeostasis of a biological system. This review compiles the role of zinc in prophylaxis/therapeutics and provides current information about its effect on living beings. © 2014 Institute of Food Technologists.
Rathaur A.K.,Babu Banarasi Das University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013
Some of the medicinal plants valued in Ayurvedic Rasayana for their therapeutic potential have been scientifically investigated with promising results. A number of plant-based principles have been isolated with potential immunomodulatory activity that can explain and justify their use in traditional medicine in the past and can form the basis for further research in the future as well. The aim of this review is to highlight results of research done on immunomodulators of plant origin. The selection of papers was made using the most relevant databases for the biomedical sciences on the basis of their ethnopharmacological use. Many plants and some phytoconstituents responsible for immunomodulation have been explained. The review also discusses biological screening methods for various plant drugs that focus on revealing the mechanism involved in immunomodulation.
Gupta S.,Babu Banarasi Das University |
Gupta R.,Babu Banarasi Das University
Asian Pacific Journal of Tropical Disease | Year: 2012
Objective: To detect and quantify the concentrations of Quercetin in the root, leaves and flower test sample of Clerodendrum infortunatum L. Method: In the present study High Performance Thin Layer Chromatography has been developed for detection and quantification of Quercetin in Clerodendrum infortunatum L. Increasing serial dilutions of reference standard Quercetin (20 to 100 μg/ml) were scanned at 366 nm to detect and quantify the concentrations of Quercetin in the test sample. Result: The estimated values obtained from the same were 0.05mg/g, 0.111mg/g and 0.199mg/g of Quercetin in powdered root, leaves and flower sample respectively. Conclusion: The method provided a rapid and easy approach for detection and the quantitation of the bio-marker Quercetin. In the present study we established the HPTLC profile for the vegetative and reproductive parts of Clerodendrum infortunatum L. to detect and quantify the Quercetin. © 2012 Asian Pacific Tropical Medicine Press.
Katiyar H.,Babu Banarasi Das University
Wireless Personal Communications | Year: 2013
Performance of cooperative relay schemes employing infrastructure based fixed relays having multiple antennas, has been investigated. Closed form expressions of outage probability, bit error rate and throughput for such system have been derived for Nakagami-m fading channels, with integer values of m. Here, relay and destination may perform either maximum ratio combining or selection combining of the signals. Comparative performance analysis of all four possible combinations has been analyzed for various relay locations, different number of antennas on the relay, various transmission rate and different fading conditions. © 2012 Springer Science+Business Media, LLC.
Dixit S.,Babu Banarasi Das University |
Katiyar H.,Babu Banarasi Das University
Proceedings - 2015 5th International Conference on Communication Systems and Network Technologies, CSNT 2015 | Year: 2015
With the growing demand for higher data rate networks, OFDM has emerged as a key technology for 4G standards like 3GPP LTE, IEEE 802.16m WiMAX and IEEE 802.11n WLAN. With its robustness to frequency selective fading, high spectral efficiency and ease of implementation by means of Discrete Fourier Transform(DFT), OFDM is a cutting edge technology for Broadband mobile wireless. Along with its compatibility with MIMO, OFDM is an attractive air-interface solution to meet the demands of 4G networks. In mobile environment, channel is subject to both time and frequency selective fading. Although OFDM is resistant to ISI resulting from frequency selective fades, but it is quite sensitive to time selective multipath fading. This time selective fading causes ICI thus degrading system performance. For a satisfactory performance of OFDM, it is imperative that the sub-carriers remain orthogonal to each other. The orthogonal behaviour of sub-carriers can be jeopardised due to two effects namely Carrier frequency offset, and Doppler spread. In the case of high Doppler spread owing to high mobility, maintaining orthogonality between sub-carriers is a challenging task. To keep a SIR of 20 dB, OFDM symbol duration must not exceed 8% of the Coherence time of the channel. In the case of Carrier frequency offset, which is caused by the use of Local Oscillators, there is a frequency shift of the received signal spectrum. This frequency shift of the received signal spectrum compromises the orthogonality between the sub-carriers. This paper reviews the methods to mitigate the deleterious effect of time selective fading and compares the simulation result of high Doppler spread with that of small Doppler spread. © 2015 IEEE.