Ayya Nadar Janaki Ammal College Autonomous

Sivakasi, India

Ayya Nadar Janaki Ammal College Autonomous

Sivakasi, India

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Vijayabaskar P.,Ayya Nadar Janaki Ammal College Autonomous | Vaseela N.,Ayya Nadar Janaki Ammal College Autonomous
Asian Pacific Journal of Tropical Disease | Year: 2012

Objective: In the present study the physico chemical characteristics, total antioxidant capacity (TAC), reducing power and the free radical scavenging potentials (DPPH radical, ABTS, H2O2 radical) of sulfated polysaccharide from marine brown algae Sargassum tenerrimum was investigated. Methods: The Sargassum tenerrimum seaweed, which have wide pharmaceutical application, were collected from the coastal region of Mandapam (Lat 09° 17'N, Long 79° 07'E), Tamil Nadu, India and evaluated for In vitro antioxidant properties. Results: The extract showed higher percentage of carbohydrate (8.20±1.23%) followed by sulphate (6.6±1.42%) and protein (0.86±0.42%). The free radical scavenging potential was found to be higher in ABTS (70.33±2.33%) followed by DPPH (64.66±2.08%) and H2O2 (61.56±2.05%). the TAC was found to be 62.55± 1.40%. The characterization of sulfated polysaccharide by FT-IR spectrum showed the presence of carboxyl, hydroxyl and sulfate groups. The structure of mobility was assed by agarose gel electrophoresis which showed highest mobility at higher pH values especially in buffer carbonate -bicarbonate (pH 10). The molecular weight of the sulfated polysaccharide was determined by gradient polyacrylamide gel electrophoresis which was found to be 40 kDa. Finally, GC-MS analysis of sulfated polysaccharide from S. tenerrimum exhibited peaks corresponding to Benzenamine (31.67%) and Aminocarb (21.45%). The overall results have established that the sulfated polysaccharide from S. tenerrimum could be used as a promising antioxidant agent. Conclusion: Physico-chemical analysis and elemental analysis of crude seaweed polysaccharide from Sargassum tenerrimum a brown algae elicited the antioxidant activity. © 2012 Asian Pacific Tropical Medicine Press.


Kannan N.,Ayya Nadar Janaki Ammal College Autonomous | Karuppasamy S.,Ayya Nadar Janaki Ammal College Autonomous
Indian Journal of Environmental Protection | Year: 2011

Present study deals with the comparison of electro coagulation treatment of color and dye with and without commercial activated carbon (CAC) and determinination of optimum conditions for the removal of gentian violet (GV) from water. this study has its own application in the removal of gention violet (GV) from industrial effuents. Experimental result will be highly useful in desiging the effuent treatment plant for the removal of dyes. Percentage of removal of gentian violet (GV) is found to increase in initial concentration and initial pH. Percentage removal of GV is found to increase in in elecrtolyte concentration, treatment time and voltage. Percentage removal of GV in presence of CAC is more than without CAC in this study. It is concluded from the result of persent study that the electro coagulation in presence of CAC could be used for removal of gentian violet (GV) from wastewater. Electro coagulation in presence of CAC is useful for the treatment of textile effuent for the removal of color and dye. © 2011 - Kalpana Corporation.


Vijayabaskar P.,Ayya Nadar Janaki Ammal College Autonomous | Somasundaram S.T.,Annamalai University
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To investigate the potent and specific anticoagulant activity of molluscan glycosaminoglycans (GAGs) isolated from whole clam tissue Donax cuneatus (D. cuneatus). Methods: Purification of few milligram quantities of GAGs from this tissue sample permitted a thorough examination of its anticoagulant activity characterization, which was partially purified by fractionation by anion exchange chromatography using DEAE cellulose column. The isolated crude and partially purified fractionated sample was showing metachromatic shift while using azure-A. The sample also exhibited prominent of biological and anti-fXa anticoagulant activity assays. Mobility was analyzed by two different buffer systems using agarose gel electrophoresis. Results: The fractionated molluscan GAG was also found to have similar peaks as that of standard heparin when assessed by the FT-IR spectrum. Finally molecular weight was determined by the gradient PAGE for crude and fractionated-II GAG, which were found to be 65 000 Da and 50 000 Da, respectively. Conclusions: The bivalve GAG was subjected to fractionation for further purification and its chemical components were analyzed. The fractionated clam heparin also showed substantial in vitro anticoagulant activity than that of commercial heparins. © 2012 Asian Pacific Tropical Biomedical Magazine.


Vijayabaskar P.,Ayya Nadar Janaki Ammal College Autonomous | Shiyamala V.,Ayya Nadar Janaki Ammal College Autonomous
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To explore the antioxidant activity of seaweed polyphenol from Turbinaria ornate (T. ornate). Methods: The brown seaweed, T. ornata was collected from Mandapam coastal region of Gulf of Mannar. It was cleaned from epiphytes, washed, shade dried and powdered. Algal extraction was carried out using methanol. The total phenol content of T. ornata was estimated by Folin-Ciocalteau method and its antioxidant activity was determined by free radical scavenging assays such as DPPH, Superoxide anion, Hydroxyl and Nitric oxide radical assay. Results: T. ornata exhibited (43.72±1.63) mgGAE/g extract of phenol content and better scavenging activity of DPPH [(84.27 ± 2.17)%], Superoxide anion [(61.86 ± 2.03)%], and Hydroxyl radical [(70.12 ± 2.03)%]. Conclusions: The presence of phenolic compounds were detected by TLC and FT-IR analysis, which revealed that the antioxidant activity might be due to the high polyphenol content of T. ornata with respect to their free radical scavenging properties. © 2012 Asian Pacific Tropical Biomedical Magazine.


Kannan N.,Ayya Nadar Janaki Ammal College Autonomous | Veemaraj T.,Ayya Nadar Janaki Ammal College Autonomous
Global Nest Journal | Year: 2010

Sorption of toxic metal ions (Ni2+, Cu2+ and Cd 2+) from Hevea brasiliensis bark carbon (HBBC) was carried out at 30±1 °C under various experimental conditions. Effect of various process parameters has been investigated by following the batch adsorption technique at 30 ±1°C. The percentage removal increased with decrease in initial concentration and particle size and increase in contact time and dose of adsorbent. As initial pH of the slurry increased, the percentage removal increased, reached a maximum. The adsorption is highly pH sensitive. Adsorption data were modelled with various isotherms and first order kinetic equations proposed by Natarajan-Khalaf, Lagergren and Bhattacharya-Venkobachar and intraparticle diffusion models found to be applicable. Kinetics of adsorption is observed to be first order with Intra- particle diffusion as one of the rate determining steps. The monolayer adsorption capacities of HBBC also studied by Langmuir isotherms. HBBCcould be used as low-cost adsorbents in effluent treatment, especially for the removal of metal ions, particular in Ni 2+, Cu2+ and Cd2+ ions. It is confirmed by FT-IR studies before and after adsorption. © Copyright 2010 Global NEST Printed in Greece. All rights reserved.


Rajagopal T.,Bharathidasan University | Thangamani A.,Ayya Nadar Janaki Ammal College Autonomous | Archunan G.,Bharathidasan University
Journal of Environmental Biology | Year: 2010

Investigations were carried out on the divemity of phytoplankton in relation to physico-chemical parametem with respect to pollution status of two perennialponds of Sattur area, Tamil Nadu. Fifty species were identified belonging to Chlorophyceae, Bacillariophyceae, Cyanophyceae and Euglenophyceae. High value of physico-chemical parameters and low phytoplankton diversity were recorded in the Chinnapperkovil pond, whereas low value of physico-chemical parameters and high phytoplankton diversity were recorded in the Nallanchettipatti pond. Class Chlorophyceae qualitatively and quantitatively dominated in both the habitats when compared to other taxa. Present study revealed that phytoplankton species richness (R1 and R2) was comparatively higher (R1:5.43±0.53; R2:3.00±0.27) in Nallancheettipatii pond. The species diversity was high (H':3.08±0.37; N1:14.05±0.63; N2:12.55±0.94) in Nallanchettipatti pond compared to Chinnapperkovil pond (H':2.25±0.15;N1:12.59±0.80;N2:8.54±0.61). Anabaena aequalis, Nitzschia bilobata, Navicula membranacea, Scenedesmus annatus, Pediastrum leonensis, Frvstulia rhomboides, Microcystis aeruginosa, Oscillatoria angusta, Closterium acerosum species dominated in Chinnapperkovil pond and Spirogyra maxima, Zygnema caeruteum and Fragilaria oceanica dominated in Nallanchettipatti pond. Abundance of such specific taxa (e.g. Closterium acerosum, C. dianae, C. lineatum, Anabaena aequalis, Oscillatoria angusta and Navicula membranacea) in the Chinnapperkovil and Nallanchettipatti (e. g. Merismopedia glauca and Fragilaria oceanica) ponds suggests that these taxa can be considered as pollution indicators. Phytoplankton species diversity and physico-chemical parameter profiles indicate the Chinnapperkovil pond to be meso-eutrophic whereas the Nallanchettipatti pond is oligo-eutrophic. ©Triveni Enterprises, Lucknow (India).


Kannan N.,Center for Research and Post graduate Studies in Chemistry | Veemaraj T.,Ayya Nadar Janaki Ammal College Autonomous
Electronic Journal of Environmental, Agricultural and Food Chemistry | Year: 2010

Batch adsorption dynamics and equilibrium studies for the removal of cadmium (II) ions from aqueous solution using indigenously prepared Eucalyptus globules bark carbon (EGBC), Bambusa glaucescens dust carbon (BGDC) and commercial activated carbon (CAC) have been carried out under various experimental conditions at 30 +1°C. Percentage removal of cadmium (II) ions increased with the decrease in initial concentration and increased with increase in contact time and dose of adsorbent. Amount of cadmium (II) ions adsorbed increases with the decrease in particle size of EGBC. Adsorption is highly pH sensitive. Adsorption data were modeled with isotherms and the first order kinetic equations. Kinetics of adsorption is observed to be first order with intraparticle diffusion as one of the rate determining steps. Removal of cadmium (II) ions by indigenously prepared activated carbons (IPACs) is found to be favourable and hence IPACs could be employed as an alternative adsorbent to commercial activated carbon (CAC) for effluent treatment, especially for the removal of cadmium (II) ions.


Rajagopal T.,Bharathidasan University | Rajagopal T.,Ayya Nadar Janaki Ammal College Autonomous | Archunan G.,Bharathidasan University | Sekar M.,Arignar Anna Zoological Park
Journal of Applied Animal Welfare Science | Year: 2011

This study investigated behavioral activities (resting, moving, aggressive, social, and reproductive behavior) and fecal cortisol levels in 8 individually identified adult male blackbucks during periods of varying levels of zoo visitors (zero, low, high, and extremely high zoo visitor density). This study also elucidated whether zoo visitor density could disturb nonhuman animal welfare. This study analyzed fecal cortisol from the samples of blackbuck by radioimmunoassay and found significant differences (p < .05) for time the animals devoted to moving, resting, aggressive, reproductive, and social behavior on days with high and extremely high levels of zoo visitors. The ANOVA with Duncan's Multiple Range Test test showed that the fecal cortisol concentration was higher (p < .05) during the extremely high (137.30 ± 5.88 ng/g dry feces) and high (113.51 ± 3.70 ng/g dry feces) levels of zoo visitor density. The results of the study suggest that zoo visitor density affected behavior and adrenocortical secretion in Indian Blackbuck, and this may indicate an animal welfare problem. © Taylor & Francis Group, LLC.


Theivasanthi T.,Ayya Nadar Janaki Ammal College Autonomous | Alagar M.,Ayya Nadar Janaki Ammal College Autonomous
International Journal of Physical Sciences | Year: 2011

This work reports a simple, novel, cost effective and eco-friendly electrolytic synthesis of copper nanoparticles using copper sulphate as metal precursor. The synthesis rate is much faster than other methods and this approach is suitable for large scale production. They are characterized by X-ray diffraction (XRD); scanning electron microscope and Fourier transform-infra red (FT-IR) techniques to analyze size, morphology and functional groups. XRD studies reveal a high degree of crystallinity and monophasic nature of copper nanoparticles. Their particle size is found to be 24 nm and specific surface area (SSA) is 28 m2/g. SSA analysis of copper nanoparticles reports that increasing their SSA improves their antibacterial actions. Microbiology essay finds that copper nanoparticles are effective against Escherichia coli and Bacillus megaterium bacteria. SSA of bacteria analysis reveals that it plays a major role while on reactions with antimicrobial agents. ©2011 Academic Journals.


Archunan G.,Bharathidasan University | Rajagopal T.,Bharathidasan University | Rajagopal T.,Ayya Nadar Janaki Ammal College Autonomous
General and Comparative Endocrinology | Year: 2013

The determination of the reproductive status is one of the most important factors for effective wild life conservation and management, and effective use of assisted reproductive techniques like artificial insemination, in vitro fertilization/embryo transfer depends on the knowledge of the basic reproductive physiology. In this context the reproductive status of female blackbucks (Antelope cervicapra L.) was assessed by behaviour and determination, sex steroid hormones in faeces and urinary volatile compounds. The male and female blackbucks exhibited as many as 31 different reproductive/courtship behaviour patterns. Particularly, the males showed a more extensive repertoire: i.e. 23 behavioural patterns by territorial males, 11 by bachelor males and 4 by females. The behaviours such as, mounting, Flehmen, clockwise and anticlockwise movements were significantly higher in male blackbuck when exposed to estrus. By contrast, such courtship behaviours were completely absent in male when exposed to diestrus. It clearly indicates that, the estrus female produces specific chemical cues (pheromone) through urine, which would involve in attracting the conspecifics. In addition, the average faecal oestrogen concentration was significantly higher (p<. 0.05) during the estrus faecal than the proestrus and diestrus periods. In contrast, the faecal progesterone concentration was significantly higher (p<. 0.05) during the diestrus faecal sample than that of proestrus and estrus faecal sample. Twenty-eight volatiles are identified, across the three reproductive phases (i.e. proestrus, estrus and diestrus) of sexually mature and prepubertal females. Amongst, the compounds 2-methyl-3-butyn-2-ol, 3,7-dimethylnonane, 3-phenyl-2-propen-1-ol and 2-hydroxybenzoic acid occurred only during estrus which may be considered as marker for detection of estrus which would ultimately help for artificial insemination in captive condition. The findings of the present study suggest that the non-invasive approaches like reproductive behaviours, faecal steroids and estrus-specific urinary volatiles could serve as good indicators for detection of estrus for blackbuck. © 2012 Elsevier Inc.

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