Baddi, India
Baddi, India

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Pankaj P.K.,Indian Central Research Institute for Dryland Agriculture | Ramana D.B.,Indian Central Research Institute for Dryland Agriculture | Srinivasa Rao C.,Indian Central Research Institute for Dryland Agriculture | Ravikanth K.,Ayurvet Ltd | And 3 more authors.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2015

Objective: A trial was conducted to study the efficacy of herbal formulation Ruchamax (M/S Ayurvet Limited, India) on digestibility and nutrient utilization in Deccani sheep. Methods: 12 growing healthy Deccani sheep (5-6 months) were randomly divided into 2 groups. Control Group I (T0) was not given any treatment. Group II (T1) supplemented with Ruchamax (appetite stimulant and digestive tonic). The test herbal formulation was mixed in the concentrate feed as per the recommendations. Parameters like body weight, plasma cortisol level, blood enzyme estimation, feed analysis, nitrogen balance, methane estimation and carcass quality traits were studied. Results: Statistically (P<0.05) Ruchamax supplemented group had significantly superior average daily gain (g) than that of control. DMI, Dry matter digestibility and FCR of Ruchamax supplemented group were significantly (P<0.05) improved. The maximum nitrogen balance was obtained in Ruchamax supplemented group T1. Significantly (P<0.05) lower level of cortisol in Ruchamax supplemented animals confirmed the increased resilience of animal towards stress. The Ruchamax supplemented group T1 emitted significantly less methane as compared to control group T0. Animals belonging to Ruchamax supplemented group T1 had significantly higher dressing % as compared to control group T0. The enzymatic values in both groups were under normal physiological level. Conclusion: The herbal appetite stimulant and digestive tonic Ruchamax was found to be safe for usage and efficient to improve digestibility, nutrient utility, performance traits in sheep alongwith added benefit of its methane mitigating potential. © 2015, International Journal of Pharmacy and Pharmaceutical Science. All right reserved.


Sonkusale P.,NVC | Bhandarker A.G.,NVC | Kurkare N.V.,NVC | Ravikanth K.,Ayurvet Ltd | And 2 more authors.
International Journal of Poultry Science | Year: 2011

A 6 week long experimental trial was conducted to study whether the supplementation of herbal products Superliv liq. and Repchol (supplied by M/S Ayurvet Limited, Baddi, India) are efficacious in treatment of liver and kidney due to CCL4 A total of 75 Cobb day old broilers were divided into three equal groups (n = 25). Group A served as negative control, group B (positive control) and C (treatment group) were induced with FLKS with CCL4 @1 ml/kg body weight orally, administered every 3rd day during 15-28 days of trial. Treated group C were given Superliv Liq. @5 ml/100 chicks/day for first 2 weeks and @ 10 ml/100 chicks/day for 3-6 weeks along with drinking water in combination with Repchol@500 gm/ton of feed from 0-6 weeks. Fatty liver and kidney syndrome lead to depression in growth rate, lowered feed efficiency and disturbances in immunopathological and biochemical parameters. Final mean body weight of group C was significantly (p<0.01) higher than group A and B. A significant (p<0.01) decrease in feed conversion ratio was observed in group C as compared to group B. A significant (p<0.01) decrease in level of cholesterol, triglycerides, HDL, VLDL, creatinine, liver enzymes (SGOT and SGPT) and increase in serum protein level was recorded in group C as compared to group B on 28th and 42nd day of experiment, which was in confirmation with the values of negative control group A, falling into normal range. Gross and histopathology of liver and kidney revealed that there was mild congestion and negligible degenerative changes in group C in contrast to severe changes of FLKS syndrome in group B. It can be concluded that liver tonic Superliv liquid along with phytoadditive Repchol (supplement for choline and biotin) efficaciously ameliorated the CCL4 induced toxicity, when administered prophylactically in broilers. © Asian Network for Scientific Information, 2011.


Yadav G.B.,P.A. College | Kadam A.S.,P.A. College | Pachpande A.M.,P.A. College | Lambate S.B.,P.A. College | And 3 more authors.
International Journal of Poultry Science | Year: 2010

Earlier the food passes through gastrointestinal tract, better is the stimulus for initiating the gut function development. Morphology of the small intestine of Vencobb broiler chick was determined immediately post hatch by comparing between untreated control group fed with standard basal ration 48 h after hatch and the treatment groups (T2 and T3) offered Chikimune at 2 different doses of 6 and 8 g/chick/day for early 2 days followed by administration of standard basal ration after 2 days. Pattern of development of the intestinal mucosa, mechanisms underlying the structural changes in small intestine were assessed. The length, weight and diameter of different parts of small intestine developed significantly earlier in treatment groups (II and III) as compared to control (T1). Crypt depth and villous height increased with age in the duodenum, jejunum and illeum. There were also significant changes in apparent villous surface area in the three regions, while interactions between age and intestinal region were significant in the case of crypt depth and villous height although, the intestinal mucosa of the strain was structurally developed at hatch, there was much change in structure with age, especially over the first 7 day post hatch. © Asian Network for Scientific Information, 2010.


Shrivastava B.,University of Delhi | Jain K.K.,University of Delhi | Kalra A.,Ayurvet Ltd. | Kuhad R.C.,University of Delhi
Scientific Reports | Year: 2014

Wheat straw was fermented by Crinipellis sp. RCK-1, a lignin degrading fungus, under solid state fermentation conditions. The fungus degraded 18.38% lignin at the expense of 10.37% cellulose within 9 days. However, when wheat straw fermented for different duration was evaluated in vitro, the 5 day fungal fermented wheat straw called here â €œ Biotech Feedâ € was found to possess 36.74% organic matter digestibility (OMD) and 5.38 (MJ/Kg Dry matter) metabolizable energy (ME). The Biotech Feed was also observed to be significantly enriched with essential amino acids and fungal protein by fungal fermentation, eventually increasing its nutritional value. The Biotech Feed upon in vitro analysis showed potential to replace 50% grain from concentrate mixture. Further, the calves fed on Biotech Feed based diets exhibited significantly higher (p<0.05) dry matter intake (DMI: 3.74â €...Kg/d), dry matter digestibility (DMD: 57.82%), total digestible nutrients (TDN: 54.76%) and comparatively gained 50â €...g more daily body weight.


Ravikanth K.,Ayurvet Ltd. | Kanaujia A.,Ayurvet Ltd. | Thakur D.,Ayurvet Ltd. | Singh P.,Ayurvet Ltd. | Gautam B.,Ayurvet Ltd.
Journal of Planar Chromatography - Modern TLC | Year: 2013

Herbal formulations have reached extensive acceptability as therapeutic agents for several diseases. The development of an authentic analytical method which can reliably profile the phytochemical composition, including quantitative analysis of marker/bioactive compounds and other major constituents, is a major challenge to scientists. With the realization of the growing popularity and demand for phytopharmaceuticals, standardization is becoming a mandatory part for the regulation of herbal drug industry. Simple, precise, and rapid high-performance thin-layer chromatographic methods were developed for the estimation of l-3,4- dihydroxyphenylalanine (l-DOPA) (I) and rutin (II), the two bioactive phytoconstituents in Methiorep premix, a phytoadditive formulation containing herbal ingredients that mimics methionine-like activity. Separation of I and II was carried out on silica gel F254 thin-layer chromatography (TLC) with butan-1-ol-acetic acid-water (8:2:2, v/v) and ethyl acetate-butan-1-ol-formic acid-water (5:3:1:1, v/v), respectively. Visualization, scanning, and quantification of analytes were performed at 288 nm and 254 nm, respectively. Linear regression analysis was used to calculate the slope, intercept, and coefficient of determination/regression coefficient (r2) for calibration plot. The response was linear in the ranges investigated. Evaluation was on the basis of peak area. The average content of these markers in different batches of the formulation was 0.28% and 0.02% (w/w), respectively. The methods were validated for linearity, accuracy, and precision in accordance with the statistical method of validation and are useful for establishing the batch-to-batch consistency.


Kumari M.,Jaypee University of Information Technology | Kumar S.,Jaypee University of Information Technology | Chauhan R.S.,Jaypee University of Information Technology | Ravikanth K.,Ayurvet Ltd
Waste Management and Research | Year: 2011

The aim of the present study was to investigate the potential of bioconversion of industrial herbal waste to vermicompost using Eudrilus eugeniae. Vermibeds were made using a mixture of herbal waste and cowdung (1 : 1) in comparison with the use of cowdung alone as substrate, resulting in vermicomposts 1 and 2, respectively. Different parameters were studied and it was observed that the nutrient profile of vermicompost 1 strongly influenced the growth of pea (Pisum sativum) and marigold plant (Tagetus erectus). The dry and fresh weight of shoots and roots, number of flowers, total yield in terms of fruit showed significant increase with vermicompost 1. Furthermore, vermicompost 1 (herbal waste and cow dung as substrate) resulted in a significant reduction in TOC by 58% in comparison with vermicompost 2 (cowdung as substrate). The C : N ratio was less than 20 in vermicompost 1 as well as in vermicompost 2, which indicated an advanced degree of stabilization and mineralization. The ability of earthworms to survive, grow and breed in the vermibed fed with the herbal waste indicates the sustainability and efficiency of a heterogeneous kind of organic waste. The results of the study suggested that bulk industrial herbal waste can be utilized as a substrate for vermicomposting and this can be proposed as an alternative for waste disposal in a clean green manner, promoting the concept of organic farming. © 2011 International Solid Waste Association.


Saxena M.,Ayurvet Ltd | Ravikanth K.,Ayurvet Ltd | Kumar A.,Ayurvet Ltd | Gupta A.,Ayurvet Ltd | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

Azadirachta indica Juss. (family Meliaceae) is a vital plant with multiple agricultural and medicinal utilities. The seed cake after oil extraction can be a good source of nutrition in animal feed. The limitation to its use is the presence of azadirachtin, salannin, and other bitter constituents. To make it palatable for use as a source of animal nutrition it was detoxified using 50 and 80% methanol and was analyzed for contents of azadirachtin, salannin, and nutritional contents such as total carbohydrates, protein, crude fiber, in vitro protein digestibility, and trypsin inhibitor activity (TIA), prior to and after purification. The contents of azadirachtin and salannin were quantified using HPTLC and HPLC. Various validation parameters were also investigated. A highly significant decrease of antinutritional factor (TIA) was recorded after purification of samples, retaining the contents of protein, carbohydrates, crude fiber, and in vitro protein digestibility. The purified seed cake was found to be free of azadirachtin and salannin contents. © 2010 American Chemical Society.


Sawarkar A.R.,Nagpur Veterinary College | Sonkusale P.M.,Nagpur Veterinary College | Kurkure N.V.,Nagpur Veterinary College | Jangade C.R.,Nagpur Veterinary College | And 2 more authors.
International Journal of Poultry Science | Year: 2011

A study was conducted in 75 dayold Vencobb broiler chicks to evaluate toxic effects of aflatoxin B1 and ochratoxin A and efficacy of herbomineral toxin binder product (Toxiroak Gold) in preventing comycotoxicosis. Chicks were randomly divided into three groups of 25 each. Group I served as healthy control (C) and given standard basal ration and no treatment, Group T 0 and T 1 comprised healthy birds fed standard basal diet and mycotoxicated with 100 ppb each of aflatoxin B1 and ochratoxin A from 0-42 days. Group T 0 is not given any treatment and served as positive control; however, mycotoxicated group T 1 was administered herbomineral toxin binder product Toxiroak Gold@1kg/tonne of feed for 6 weeks. Mycotoxin adversely affected body weight gain, feed consumption, feed efficiency, haematobiochemical profile. However, supplementation of herbomineral toxin binder feed supplement has provided amelioration in mixed mycotoxicosis in broilers. © Asian Network for Scientific Information, 2011.


Ravikanth K.,Ayurvet Ltd | Singh B.,Ayurvet Ltd | Gupta A.,Ayurvet Ltd | Singh A.,Ayurvet Ltd | And 2 more authors.
Journal of Planar Chromatography - Modern TLC | Year: 2011

With the realization of the growing popularity and demand for phytopharmaceuticals, standardization is becoming a mandatory part of the regulation of the herbal drug industry. This is applicable both to humans and veterinary formulations. Stresroak premix is a renowned polyherbal formulation for poultry. It is used as an immunomodulator, an antistress treatment, an adaptogenic, and a performance enhancer. It is a blend of Phyllanthus emblica L. (Euphorbiaceae), Withania somnifera L. (Solanacaeae), Mangifera indica L. (Anacardiaceae), Ocimum sanctum L. (Lamiaceae), and Shilajit. An HPTLC method has been established for standardization and quantification of gallic acid, mangiferin, and withanolide A in Stresroak premix. The average content of these markers in different batches of the formulation was 0.654, 0.627, and 0.325% (w/w), respectively. The method was validated for instrumental precision, repeatability, and accuracy. Average recovery of gallic acid, mangiferin, and withanolide Awas 100.41, 99.29, and 98.04% (w/w), respectively. The method is simple, precise, specific, and accurate and has potential for use in the routine quality control of the formulation and its raw materials. © Akadémiai Kiadó, Budapest.


PubMed | Ayurvet Ltd. and University of Delhi
Type: | Journal: Scientific reports | Year: 2014

Wheat straw was fermented by Crinipellis sp. RCK-1, a lignin degrading fungus, under solid state fermentation conditions. The fungus degraded 18.38% lignin at the expense of 10.37% cellulose within 9 days. However, when wheat straw fermented for different duration was evaluated in vitro, the 5 day fungal fermented wheat straw called here Biotech Feed was found to possess 36.74% organic matter digestibility (OMD) and 5.38 (MJ/Kg Dry matter) metabolizable energy (ME). The Biotech Feed was also observed to be significantly enriched with essential amino acids and fungal protein by fungal fermentation, eventually increasing its nutritional value. The Biotech Feed upon in vitro analysis showed potential to replace 50% grain from concentrate mixture. Further, the calves fed on Biotech Feed based diets exhibited significantly higher (p<0.05) dry matter intake (DMI: 3.74 Kg/d), dry matter digestibility (DMD: 57.82%), total digestible nutrients (TDN: 54.76%) and comparatively gained 50 g more daily body weight.

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