ATLANTA, GA, United States

Axion Biosystems, Llc

www.axionbio.com
ATLANTA, GA, United States
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Patent
Axion Biosystems, Llc | Date: 2016-06-06

An electro-optical stimulation and recording system is disclosed, including a substrate and a plurality of wells coupled to the substrate. The system also includes at least one electrode set disposed proximate a respective one of the plurality of wells, wherein the electrode set comprises at least one electrode configured to collect an electric signal associated with at least a portion of the respective well. The system also includes a light-emitting element set corresponding to a respective one of the wells and configured to deliver optical stimulation to at least a portion of the respective well.


Devices, systems, and methods for facilitating placement of cells and materials in culture plates configured for high-throughput applications are provided. A culture system is provided with a culture plate having a lid for guiding placement of cells and materials in each individual culture well of a culture plate. The lid may provide for coupling to an electrophysiology culture plate comprising a biosensor plate and a biologic culture plate, where the biosensor plate underlies and is coupled to the culture well plate such that each biosensor is operatively coupled to one culture well of the plurality of culture wells. A containment device that physically influences the positioning of fluid received in the culture plate is also provided herein.


Patent
Axion Biosystems, Llc and Georgia Institute of Technology | Date: 2015-02-02

A neural interfacing device is disclosed. The neural interfacing device may include at least one microneedle electrode. The microneedle electrode may have one or more microneedles. The one or more microneedles may be shaped and positioned such that when the neural interfacing device is applied to a nerve, the one or more microneedles penetrate a nerve epineurium without any portion of the microneedle electrode penetrating any nerve axon beyond a depth of 500 micrometers.


Patent
Axion Biosystems, Llc | Date: 2016-02-11

An electro-optical stimulation and recording system is disclosed, including a substrate and a plurality of wells coupled to the substrate. The system also includes at least one electrode set disposed proximate a respective one of the plurality of wells, wherein the electrode set comprises at least one electrode configured to collect an electric signal associated with at least a portion of the respective well. The system also includes a light-emitting element set corresponding to a respective one of the wells and configured to deliver optical stimulation to at least a portion of the respective well.


A cell-based biosensor array includes a base plate having a plurality of substantially transparent areas. The cell-based biosensor array also includes a flexible substrate coupled to the base plate and having disposed thereon a plurality of electrode sets, a plurality of terminal contacts, and a plurality of conductive traces. Each electrode set is disposed proximate a respective one of the substantially transparent areas, and each electrode set includes at least one electrode configured to detect an electric signal. Each terminal contact is associated with a respective one of the at least one electrode and disposed proximate a perimeter of the flexible substrate. Each conductive trace is electrically coupling a respective at least one electrode to the corresponding terminal contact. A first portion of flexible substrate including the electrode sets is disposed on a first surface of the base plate. A second portion of the flexible substrate including the terminal contacts is disposed on a second surface of the base plate.


Disclosed herein are systems and methods for assessing electrically active cell cultures. Optionally, the data can be collected using a microelectrode array (MEA). For example, electrically active cells, such as cardiomyocytes, are cultured such that they are in electrical communication with at least a portion of the electrodes of a well of the MEA. The assessments derived from the disclosed methods may be used to reduce the effects of confounding variables in data obtained from an electrically active cell culture. The methods may also be used to determine a quantitative measure of arrhythmia burden. The methods may also be used to decide if a particular culture or set of data is suitable for inclusion in scientific and characterization studies. Also disclosed is a method of finding the global conduction velocity of an electrically active cell culture.


Patent
Axion Biosystems, Llc | Date: 2015-06-12

An electro-optical stimulation and recording system is disclosed, including a substrate and a plurality of wells coupled to the substrate. The system also includes at least one electrode set disposed proximate a respective one of the plurality of wells, wherein the electrode set comprises at least one electrode configured to collect an electric signal associated with at least a portion of the respective well. The system also includes a light-emitting element set corresponding to a respective one of the wells and configured to deliver optical stimulation to at least a portion of the respective well.


Patent
Axion Biosystems, Llc | Date: 2015-06-19

Electrophysiology culture plates are provided and are formed from a transparent micro-electrode array (MEA) plate. The MEA plate comprises a substrate, a first layer and a first insulating layer. The substrate has a plurality of vias extending from an upper to a lower surface, each via being in electrical contact with each of a plurality of contact pads disposed on the lower surface. The first layer is disposed on the upper surface of the substrate and has a plurality of MEA arrays in in electrical communication with at least a first routing layer. Each MEA array comprises a plurality of reference electrodes and a plurality of microelectrodes and the first routing layer is in electrical communication with a select number of the plurality of vias. A first insulating layer is disposed on the first layer. The MEA plate is joined to a biologic culture plate having a plurality of culture wells such that each culture well defines an interior cavity having a bottom surface that is at least partially transparent and in positioned in registration with a select optical port. The MEA plate is coupled to the biologic culture well plate such that each MEA array is operatively coupled to one culture well wherein each microelectrode and each reference electrode are in electrical communication with the interior cavity through the bottom surface of the culture well.


Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase I | Award Amount: 156.50K | Year: 2016

DESCRIPTION provided by applicant This proposed project will develop and validate a microhook based nerve tape device for quick and effective repair of nerve injuries The microhook wrap consists of an array of microscale hooks distributed across a fine flexible meshwork and embedded in a biocompatible backing material This nerve tape will be used during nerve repair surgeries in place of microsutures to wrap around and bind the coapted ends of a severed nerve The short microhooks penetrate only into the outer connective tissue layers of the nerve forming an effective mechanical connection akin to VelcroTM brand products This mechanical connection distributes tension evenly across the wrap and can be applied quickly and reproducibly without the need for a surgical microscope or extensive microsurgical training Versus the current clinical standard microsuture repair microhook nerve tape has the potential to reduce overall surgical costs and enhance recovery after nerve injuries Phase I will optimize the design of microhook nerve tape and validate its safety and efficacy Specifically Aim will test various configurations of microhooks backing materials and closure methods Aim will use biomechanical testing on human cadaver nerves and rabbit implantation models to validate the ability of the nerve wrap to engage epineurial tissue without chronic inflammation or negative effect on regeneration axons Phase II will include extensive animal and clinical testing of functional outcome measures establishment of manufacturing processes and ultimately k approval and licensing distribution agreements The final product will be an FDA approved andquot microhook nerve tapeandquot material that can be stored on the shelf for use during nerve repair procedures Once packaged as a commercial medical device microhook wrap will reduce healthcare costs while improving patient access and quality of recovery PUBLIC HEALTH RELEVANCE This project will develop a microhook based nerve tape material for wrapping around and holding together the two ends of a cut nerve The distributed array of microscopic hooks bind strongly to the outer tissue layers without penetrating to the delicate interior This repair can be performed quickly and effectively and has the potential to improve nerve regeneration Microhook nerve tape will lower the time and cost of nerve repair surgeries and enhance patient recovery from debilitating nerve injuries


Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase II | Award Amount: 2.26M | Year: 2013

DESCRIPTION provided by applicant In response to the program announcement for Phase IIb proposals for complex in vitro brain and behavior tools PA this proposed study automates all aspects of in vitro Microelectrode Array MEA technology to deliver the first ever high throughput MEA based screening system This development will not only enable rapid advancements in the study of basic network level electrophysiology but it will also create new opportunities for CNS disease modeling and phenotypic screening This Phase IIb proposal involves two significant developments for neurological research both building on the commercialized channel multiwell MEA system developed in Phase II Specifically Aim automates compound administration and experiment management to produce a turnkey platform for dosing neural culture as well as capturing com pressing and storing network electrophysiological datasets Among its many advantages this platform will recover signals traditionally obscured by stimulation artifacts This capability combined with the ability to simultaneously manage microelectrodes and automate experimental protocols will capture single cell and network level neural activity at unparalleled rates In addition Aim will automate the analysis of the immense volumes of continuous data traces generated by the Aim developments translating massive raw datasets into meaningful metrics that accurately reflect the interactions between networks of cells Ultimately the final MEA screening platform will provide automated liquid handling user friendly experiment tools and sophisticated neural analysis software For scientists this integration will significantly improve their ability to extact functional information from neural cultures paving the way for new progress in drug discovery safety assessment and basic science PUBLIC HEALTH RELEVANCE This research uses novel software analysis tools and automated liquid handlers to create faster lower cost methods for neural research Ultimately this development will facilitate medical and scientific discoveries that will benefit the treatmentof neural disorders such as Parkinsonandapos s disease and epilepsy

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